Biological Therapy ; Carcinoma, Neuroendocrine ; Thyroid Neoplasms ; therapy

Hepatic Encephalopathy ; classification ; diagnosis ; therapy ; Humans ; Terminology as Topic

Based on the reality of Anhui Medical University,the paper analyzes the adverse factors of carrying out combination with teaching for the literature retrieval course from the perspectives of emphasis,professional knowledge structure and teachers of professional courses,and puts forward corresponding countermeasures from implementation of teaching and teaching effect,in order to provide reference for medical colleges to carry out literature retrieval course effectively.

BACKGROUND: Transplantation of the human umbilical cord blood mononuclear cells (HUCBCs) have received increasing attention, as a promising candidate for the cellular transplantation, but the majority of the existing studies are basic research.OBJECTIVE: To report a patient of extensive anterior-wall acute myocardial infarction with cardiogenic shock and severe heart failure, after treatment of HUCBCs transplantation. METHODS: A 73-year-old female patient with cardiogenic shock and severe heart failure after extensive anterior-wall acute myocardial infarction was treated with percutaneous coronary intervention (three scaffolds implantation) and medications, and she still appeared the symptoms of congestive heart failure, such as severe recurrent dyspnea. 2.4 × 108 HUCBCs (50 mL cell suspension) was injected into the infarcted myocardium through the left anterior descending artery by using coronary micro-guide catheter.RESULTS AND CONCLUSION: The patient reported profound clinical benefit including improvement of heart-failure-associated symptoms after the transplantation. Notably the patient did not experience the cell transplant-related side effects during 4 months of follow-up. The ejection fraction increased from 22% before the transplantation to 53% at 21 days after the transplantation. The B-type natriuretic peptide decreased from 1 730 ng/L before the transplantation, 854 ng/L after the transplantation to 264 ng/L at 21 days after the transplantation. The patient did not appear the symptoms of congestive heart failure, including dyspnea, chest distress and hypodynamia, she returned to daily activity at 4 months of follow-ups. Experimental findings indicate that the HUCBCs transplantation is an effective and safe means for patients cardiogenic shock and severe heart failure after acute myocardial infarction.

By means of the observation of acridine orange-stained lysosomes with fluorescence microscope, we studied the intracellular killing and digestive process after alveolar macrophage (AM) ingested living-yeast. Based on the morphological changes of the cells, we divided the process into three stages: the pre-phagosome-lysosome fusion stage, the phagosome-lysosome fusion and killing stage, and the digestive stage. The results revealed that all the values of phagocytic index, phagocytic rate, fusion index and fusion rate of the isolated AM of rabbits infected with living-BCG were higher than those of normal rabbits (P

Alveolar macrophages (AM) were studied by means of phase contrast microscope, differential interference contrast microscope, SEM and TEM. The content of AcP, LDH, SDH of the AM was measured by MPV3-TSA microspectrophotometerimage analytical instrument. Under phase contrast microscope, two different types of AM can be distinguished, i. e, the light cells and the dark cells. The two types of cells are spherical and flat in shape under differential interference contrast microscope. The observation with SEM and TEM showed that the spherical cells possess more filopodia and more lysosomes in cytoplasm, but the flat cells possess more lamellar podia. Quantitative cytochemistry demonstrated that the content of AcP, LDH, SDH in spherical cells are much more than that of flat cells. The results suggest that there are two types of alveolar macrophages exist in normal rat lung.

The phagocytic ability and fashion of two types of alveolar macrophages (AM) on coal dust were studied by differential interference contrast microscope, scanning electron microscope and image analyser. This study revealed the phagocytic ability of spherical AM on dust was higher than that of the flat AM. Both types of AM could extend long tenuous filopodia to the coal dusts around the cells. The coal dusts were attached to the cell surface and then were sunk into the macrophage. The amount of the phagocyted coal dusts per unit time were measured by image analytical method which is more exact than other methods. This paper provided not only a new approach for study phagocytosis of macrophage on inorganic dusts, but also some essential morphologic evidences for study of pneumoconiosis.

Objective To observe the effect of ulinastatin on NT -proB -NP and Picco in patients with severe sepsis.Methods 50 patients with severe sepsis were randomly divided into the control group and observation group,25 patients in each group.The control group received the routine treatment,and the observation group received ulinastatin additionally.The venous blood was collected to determine cTn -I,NT -proBNP,TNF -αand Lac before treatment and 7 days after treatment.The APACHE Ⅱ and CFI were observed before treatment and 7 days after treat-ment.Results The differences of CFI,cTnI,NT -proBNP in the two groups before treatment were not significant (t =0.419,0.321,0.335,all P >0.05),after treatment,cTnI and NT -proBNP decreased more significantly(t =2.976,2.910,all P <0.05),while lifting more apparent CFI[(3.98 ±0.95)vs.(2.39 ±0.85),t =6.237,P <0.05]in the observation group than in the control group.The differences of TNF -αand Lac in the two groups before treatment were not significant (t =0.602,0.848,all P >0.05 ),after treatment,TNF -αand Lac decreased significantly faster in the observation group than in the control group(t =8.478,3.104,all P <0.05).After treat-ment,APACHE Ⅱ score decreased significantly in observation group than in the control group(t =2.321,P <0.05). Conclusion Ulinastatin had protective effects on heart function in patients with severe sepsis.

Objective:To investigate the effect of GRP94 gene expression down-regulation on the proliferation in human lung carcinoma A549 cells and possible mechanism.Methods:Expression of GRP94 was examined in 4 lung carcinoma cells and two lung bronchial epithelial cells by qRT-PCR and Western blot.Chemically synthesized siRNA targeting GRP94 gene and transfected into A549 cells used by Lipofectamine2000.The mRNA and protein expression levels of GRP94,c-myc and cyclinD1 were detected by qRT-PCR and Western blot.CCK8 assay and Colony formation assay were used to detect the effect of specific GRP94 siRNA on cell proliferation.Results:The levels of GRP94 expression in the four lung carcinoma cells were significantly higher than that in two lung bronchial epithelial cells (P<0.05).GRP94 siRNA significantly downregulated the levels of GRP94 mRNA and protein in A549 cells, and the downregulation evidently suppressed cell proliferation.However, The c-myc and cyclinD1 expression levels in A549 cells in siRNA-GRP94 group was significantly lower than that in siRNA-NC group (P<0.05).Conclusion:The levels of GRP94 expression in lung carcinoma cells were significantly higher than that in normal lung epithelial cells.Knockdown GRP94 expression significantly inhibit the level of lung carcinoma A549 cells proliferation,which may act via influencing the expression of c-myc,cyclinD1.

Exosome is a sort of vesicle structure attached to cell membrane and released by cells, which contains numerous bioactive molecules, such as proteins, lipids, mRNA, microRNA and DNA fragments. These bioactive molecules are involved in the regulation of many biological processes, not only the participation in the occurrence and development of diverse diseases, but also the close relationship with the invasion and metastasis of tumors. The recent study has indicated that lots of exosomes released from cancer cells participate in a variety of pathological processes as a messenger of cell functions. This article will elaborate the function and application of exosomes in some malignant cancers.