According to the national residency training regulations in the teaching of clinical and scientific research, the department of thoracic surgery of First Affiliated Hospital of Chongqing Medical University has used seminar teaching , and has collected random sampling survey questionnaire of 110 students participating in seminar from September 2014 to March 2016. The investigation shows that through seminar teaching training, doctors will cultivate their clinical and research ability, and enhance their self-confidence. Therefore, the application of seminar teaching in department of thoracic surgery is helpful to improving the quality of clinical teaching and is worthy of further improvement and promotion.

As an advanced quantitative technology for cells, flow cytometry, which can make multi-paramter quantitative analysis and sorting of individual cells or biological particles, has been widely used in immunology, oncology, hematology and other fields of medical research and clinical diagnosis in recent years.It can quantitatively detect the expression levels of cytokines and the distribution characteristics of cell subsets in the intraocular fluid.Compared with traditional enzyme-linked immunosorbent assay, cell morphology and immunohistochemistry, it has the advantages of simple operation, smaller sample size, higher sensitivity and higher throughput.Flow cytometry has been widely used in the detection and subgroup analysis of cytokines related to eye diseases, such as intraocular angiogenesis, intraocular lymphoma, retinopathy, cataract, uveitis, sarcoidotic uveitis, infectious intraocular inflammation, etc.Flow cytometry plays an increasingly important role in the pathogenesis research and targeted therapy of eye diseases.In this article, the application of flow cytometry in the examination of cytokines and cytology in ocular fluid for ocular diseases were reviewed.

The 109th Annual Meeting of the American Urological Association was held in Orlando, Florida, USA in May 2014, which received more than 1,000 abstracts on prostate cancer (PCa), covering new epidemiological data about PCa, new theories of early screening, novel molecular markers, new surgical methods, new ideas of diagnosis and treatment of castration-resistant PCa, and progress in basic researches. This paper focuses on the new theories of early screening and novel molecular markers of PCa, including the risk factors of PCa, a revolutionary understanding of the relationship between testosterone and PCa, and new application of PSA, new imaging techniques and molecular markers in the early diagnosis of PCa.
Biomarkers, Tumor ; blood ; Early Detection of Cancer ; Florida ; Humans ; Male ; Prostatic Neoplasms ; blood ; diagnosis ; therapy ; Risk Factors ; Societies, Medical ; Testosterone ; blood ; United States ; Urology

Objective To investigate and analyze structure of risk perceived by people using HIV voluntary counseling and testing services and related factors.Method Two hundred and sixty eight VCT users were selected from 2 CDCs and 2 hospitals in Beijing and their perceived risks in the process of VCT were assessed by using self-administered question- naires.Factor analysis was performed to understand the relationship between different perceived risks,and risks perceived by different users were evaluated.Results Six factors were obtained from the factor analysis which included:fear of priva- cy disclosure,embarrassment,concern over the reliability of HIV tests,cost & time consumption,the tragic outcomes of HIV positive tests,and potential medical expenditures in the future.And the risks perceived by low and high income users were much higher than those perceived by middle income users.Conclusion There are clear structure of risks perceived by people using HIV voluntary counseling and testing,and people with different incomes have different levels of perceived risks.

Humans ; Functional Laterality ; Forensic Medicine ; Autopsy ; Asphyxia/etiology*

Objective To assess the impact of recombinant pigment epithelium-derived factor (rPEDF) on the growth and invasion of the glioma cells.Methods The level of vascular enthothelial growth factor (VEGF) and thrombospondin-1 (TSP-1) were evaluated by enzyme-linked immunoadsorbent assay (ELISA); cell proliferation assays were performed by MTT assay; the cell migratory assay was used for evaluating its inhibitory migration rate.Results The VEGF expression (0.29?0.03) of A172_ PEDF (added the PEDF to the A_ 172 cells) decreased 4.4 times compared with the A172_ con (not added the PEDF to the A_ 172 cells, 1.31?0.02, P

Objective To evaluate the effectiveness and safety of a new designed balloon catheter, the Minirail balloon catheter in coronary intervention Methods Twenty two consecutive patients with coronary artery disease (male 16, female 6, mean age 58 77?8 69 years) were enrolled the study Selective angioplasty was performed for 24 target lesions (17 in LAD, 2 in LCX, and 5 in RCA) Among these target lesions were 5 type C, 14 type B 2, and 5 type B 1 lesions The average extent of narrowing before procedure was 91 18%?5 95% Target lesions were dilated with Minirail balloon catheters Results All of the target lesions were successfully dilated with Minirail balloon catheters The average residual stenosis was 33 18%?25 14% after balloon angioplasty Significant dissection was seen in 3 of the cases, and slow reflow in another one Twenty four stents were implanted in 19 cases The final residual stenosis was 1 82%?5 01% at the end of procedure No death or Q wave myocardial infarction happened No emergency bypass surgery needed Conclusion Minirail balloon catheter is safe, effective, and easy to be used in coronary intervention

Objective:to study cognitive impairment and social function of patients with Alzheimer disease (AD). Method:22 patients with AD and 12 with depression in elderly were followed for 3 months, both at the very beginning and the end of 3 months, they were tested with CASI (cognitive abilities screening instrument) and FAQ (functional activities questionnaire).Result:scores of short memory, attention, mathematics, abstract and association of CASI were significantly lower in AD group than that in depression group. AD patients had also poorer outcome in FAQ, such as in that of shopping, learn new things, and recall of engagement.Conclusion:Cognitive impairment and social function of patients with AD were poorer than elderly patients with depression.

Objective To evaluate the efficiency and safety of Percusurge guardwire distal protection system in coronary intervention in patients with acute myocardial infarction. Methods Three male patients with acute myocardial infarction were enrolled in the study. Primary angioplasty was performed for 2 of them, 2 and 7 hours after onset of the infarction symptom, respectively. Selective angioplasty was performed 17 days after infarction for the third case. Percusurge guardwire system was used during the procedure. Results Procedure success was achieved for all of the 3 cases. Lot of thrombus was sucked out. The infarct-related arteries had TIMI 3 flow at the end of the procedures. The patients′ symptoms were resolved soon after the procedure. Minor dissection was seen in one case which did not obstruct the distal flow and was left alone. Conclusion Percusurge guardwire system can be used safely and effectively in coronary intervention in the thrombus-laden arteries such as acute myocardial infarction.

Objective:To investigate the clinical efficacy and compliance with a new antidepressant mirtazapine to treat depressive patients.Methods:A open-labeled study was conducted and followed up six months for 60 depressive patients treated with mirtazapine, 62 cases with depression, as a control group, treated with Clomipramine and Fluoxetine, which were assessed by Hamilton Depression Rating Scale(HAMD), Asberg Rating Scale for Side Effects(SERS), Clinical Global Impression(CGI) and Attitudes Questionaire made by ourselves. Results:The group treated with mirtazapine was superior to a control group in aspect of responsive time, early variety of factors in scales, side effects, prescription compliance , treatment attitudes and falling off cases(P