Methylated DNA is considered as a new generation of tumor biomarker.It shows great value for evaluation of cancer risk,early detection and predicting prognosis.MS-HRM is a novel approach for quantitative assessment of methylation.It is based on the different base compositions after PCR amplification of bisulfite-modified DNA template,which give rise to different thermal properties of the PCR products originating from methylated or unmethylated target template.This article describs the advantages of this method and its role in the detection of cancer and inherit disease.The prospective application in the molecular diagnosis is also suggested.

Absence of clinical laboratory technician/technologist certification and registry,which leads to a variety of problems in the reliability of test results and staff management and administration,remains the main obstacle in the development of laboratory medicine in China.Here the official institution of educational background requirement,certification and license,continuing education and job vacancy of clinical laboratories in the United States were described briefly in order to appeal for the implementation of practice access in China.

Molecular diagnostics is now playing an increasingly important role in the diagnosis , prognosis and therapeutic monitoring of diseases .However, the molecular diagnostic practices in China are still lagging far behind those in the western countries , such as levels of testing platform , test diversity , quality control , result interpretation and administration of laboratory developed tests .Here we compared the current situation of molecular diagnostics in China with the daily work flow in the advanced countries , appealing for the collaboration of governmen administrators , professional associations and clinical laboratories to establish the appropriate supervising policies , technical guides and quality control standards of molecular diagnostic tests .On the premise of an ensured test result , the opening up of clinical test administration will effectively accelerate the development of molecular diagnostics .

College of American Pathologists (CAP) and International Standard Organization (ISO)15189 accreditation provides requirements to the verification and validation tests for clinical molecular diagnostic assays.The article will introduce the requirements in these aspects including the differences between the two accreditation systems,the differences between the verification and validation tests,the essential factors and standardized framework of the verification and validation tests for clinical molecular diagnostic assays.

High-resolution melting technique shows great potential as a clinical diagnostic tool because of its cost-effectiveness , convenience and closed-tube format which can prevent the risk of cross contamination.HRM analysis has been widely used in molecular diagnostics in the management of cancer by mutation detection , pre-sequence screening , methylation and copy number variations , which involves cancer screening, diagnosis, individual treatment and prognosis .Take advantage of the high detection sensitivity of HRM, the accuracy of mutation screening method could be improved and the test cost could be reduced by combing HRM analysis with sequencing , which helps to develop the molecular diagnostic technique .

[Objective] To explore the relationship between Chinese traditional medicated food science and modern nutriology, identify the developmental direction of modern medicated food culture, and propose suggestions for the scientific development of medicated food culture. [Method] Based on the previous studies, the comparative research was conducted to analyze the difference between and similarity of medicated food science and modern nutriology in terms of the theory system and research expertise. [Result] The scientific and objective recognition of the effects of medicated food could promote the development of medicated food culture. For the academic research, the advantages of traditional medicated food culture and weakness of modern nutriology need to be understood. For the practice, the experience of modern nutriology can be learned to help the innovation of medicated food science's research methods and regulation of usage. [Conclusion] The development of medicated food culture not only needs to inherit the tradition, but also needs to learn from the modern nutriology and pharmacology, and assimilates the achievements from modern western science civilization.

Nowadays,Traditional Chinese Medicine schools meet the embarrassment during their development,such as:the deposal of Traditional Chinese Medicine;herbalist doctors' cultivation;human resource supply;management of Traditional Chinese Medicine Schools.The paper suggests that Traditional Chinese Medicine Schools should follow such development strategy as giving prominence to its characteristics by setting cross-subjects,perfecting positioning,strenthening internationalization and developing lifelong education.

Animals ; Humans ; Spectrometry, Mass, Electrospray Ionization ; Spectrophotometry, Atomic ; Thallium ; analysis ; metabolism

The 109th Annual Meeting of the American Urological Association was held in Orlando, Florida, USA in May 2014, which received more than 1,000 abstracts on prostate cancer (PCa), covering new epidemiological data about PCa, new theories of early screening, novel molecular markers, new surgical methods, new ideas of diagnosis and treatment of castration-resistant PCa, and progress in basic researches. This paper focuses on the new theories of early screening and novel molecular markers of PCa, including the risk factors of PCa, a revolutionary understanding of the relationship between testosterone and PCa, and new application of PSA, new imaging techniques and molecular markers in the early diagnosis of PCa.
Biomarkers, Tumor ; blood ; Early Detection of Cancer ; Florida ; Humans ; Male ; Prostatic Neoplasms ; blood ; diagnosis ; therapy ; Risk Factors ; Societies, Medical ; Testosterone ; blood ; United States ; Urology

Adult ; Glottis ; pathology ; Humans ; Laryngeal Neoplasms ; pathology ; Lymphoma, Non-Hodgkin ; pathology ; Male ; Tracheal Neoplasms ; pathology