Objective To explore the inhibitory effects of RNAi targeting survivin to suppress the expression of survivin and to induce the apoptosis of human renal cancer cell line 786-O.Methods The target gene fragments were cloned into pcDNATM 6.2-GW/EmGFPmiR vectors,and then the recombinant eukaryotic expression vectors were transfected into 786-O cells with liposomes.The expression changes in survivin mRNA and protein were detected by RT-PCR and immunohistochemical assay,and the proliferation inhibitory rate (IR) of 786-O cells was detected by M'TT assay.Results The mRNA and protein expressions of survivin in the siRNA group were significantly inhibited by the eukaryotic expression vectors,and it also inhibited significantly the growth and proliferation of 786-O cells.Conclusion Silencing survivin gene by the RNAi technique can not only effectively decrease the expressions of survivin gene and protein,but also inhibit the cell proliferation in 786-O cells.This may provide the experimental evidence for the survivin gene as a target of gene therapy in renal cancer.

[Objective] To observe the clinical efficacy of ZICAOYOUSHA in treating diabetic foot ulcers.[Method] A multi-center ,randomized,double-blind,placebo-control ed study was conducted. A total of 232 patients with diabetic foot ulcers were randomly assigned the treatment group and control group,in foundation treatment at the same time,the therapy group which was treated by External Application ZICAOYOUSHA had 174 patients,the contrast group which was treated by External Application Gentamicin Emery cloth had 58 patients. Observe the aspect improvement situation in two groups separately in accordance with Wagner grading,carry out statistics processing.[Results] Two groups of curative effect indices had significant differ-ence. [Conclusion] ZICAOYOUSHA is an effective drug for external use in treating diabetic foot ulcers.

Female ; Fertilization in Vitro ; Humans ; Integrative Medicine ; Medicine, Chinese Traditional ; Ovary ; drug effects ; Ovulation Induction ; methods ; Pregnancy ; Pregnancy Rate

Angiogenesis Inhibitors ; adverse effects ; therapeutic use ; Angiotensin-Converting Enzyme Inhibitors ; therapeutic use ; Antibodies, Monoclonal ; adverse effects ; therapeutic use ; Antibodies, Monoclonal, Humanized ; Aspirin ; administration & dosage ; therapeutic use ; Bevacizumab ; Hemorrhage ; chemically induced ; Humans ; Hypertension ; chemically induced ; drug therapy ; Intestinal Perforation ; chemically induced ; surgery ; Neoplasms ; drug therapy ; Proteinuria ; chemically induced ; Thromboembolism ; chemically induced ; drug therapy

The voice is a complex multidimensional phenomenon. It is a drive through the lung airflow to vibrate the vocal cords, and then be perceived by the listeners. A multidimensional set of minimal basic measurements suitable for the voice diseases are proposed by the Committee on Phoniatrics of the European Laryngological Society in 2000. It includes five different aspects: the subjective physician perception (grade, roughness, breathiness), and subjective rating by the patient, videostroboscopy (closure, regularity, mucosal wave and symmetry), acoustics analysis and aerodynamics measurements. The first three are the subjective assessment of voice, these are not to be discussed in tis article. Only objective assessments of the voice and the latest research progress are summarized below.
Acoustics ; Humans ; Vocal Cords ; Voice Quality

Objective To investigate the application status of aspirin for secondary prevention among elderly patients with ischemic stroke in some community hospitals in Shanghai,and explore the main causes for inappropriate use of aspirin and the effect of aspirin on the prevention of ischemic stroke recurrence. Methods The cross-sectional investigation was employed,and 223 patients from 10 community hospitals of Shanghai were divided into regularly-received aspirin group(n=98) and irregularly-received aspirin group(n=125).The application status of aspirin was investigated and the relationship between aspirin application and ischemic stroke recurrence was explored. Results Fifty-one cases in irregularly-received aspirin group(40.80%) stopped aspirin use or reduced the dose due to possible adverse effects,which accounted for 22.87% of the total population investigated,and 42 cases in irregularly-received aspirin group(33.60%)were never suggested to use aspirin or only used traditional Chinese medicine.The rate of ischemic stroke recurrence was 30.61%(30 cases) in regularly-received aspirin group,and 49.60%(62 cases) in irregularly-received aspirin group,which existed significant differences between these two group(P

Abscess ; diagnosis ; microbiology ; surgery ; Bacteria, Aerobic ; isolation & purification ; Child, Preschool ; Female ; Fever ; Humans ; Prognosis ; Spleen ; pathology ; surgery ; Splenic Diseases ; diagnosis ; surgery ; Treatment Outcome

Objective To investigate the apoptosis induction of Bullatacin on A549 cell line of pulmonary adenocarcinoma. Methods The MTT assay was used to detect the growth inhibition rates of A549 cells cultured with Bullatacin in different concentrations (6.25, 12.5, 25, 50, 100μg/mL). 25μg/mL Bullatacin was used to culture A549 cells for 0, 12, 24, 48 h. The cell cycle distribution and apoptosis were measured by flow cytemetry. The protein expressions of ERK, JNK, and p38 were studied by Western blot. Results Dosage dependence was obviously showed after the different concentrations of Bullatacin were used to A549, and 25 μg/mL;Bullatacin blocked A549 cell in G0/G1 periods and induced its apoptosis. Compared with the blank group, protein expressions of P-ERK, P-JNK, and P-p38 were all increased by different degrees. Conclusion Bullatacin significantly inhibits the proliferation and induces the apoptosis of A549 cell. Its mechanism is related to activity of MAPK pathway thought the phosphorylation of the three protein kinases by Bullatacin.