Because of the factors such as degeneration or congenital dysplasia which leads to medial tibiofemoral compartment tightness, the arthroscopic surgery of this part might have limited vision and narrow operating space, which causes missed diagnosis and iatrogenic injury.Medial collateral ligament ( MCL) release technique is a simple, effective and safe procedure.By releasing part of the ligament fiber, this operation can effectively expand the visual field and operation space.avoiding the risk of iatrogenic injury of cartilage and missed diagnosis.MCL release technique is an effective method to solve the arthroscopic examination and endoscopic operation for medial compartment tightness of the knee joint.This article reviewed the technique of MCL release.

Objective To observe the effects of termination of pregnancy with Guizlfi Fuling Capsule plus Mifepristone.Methods 75 early pregnant women were randomly recruited into a treatment group and a control group.Women in the control group were applied with Mifepristone plus Misoprostoi.Form the 3rd day,women in the treatmcnt group were applied wilh Guizhi Fuling Capsule(3 times daily,and 3 granules for each time)for successive 7 days oil the basis of medicines used in the control group.Compared and analyzed the therapeutic effects of both groups.Results The average time for ovum evacuation in the control group and the tretment group was(6±1.8)h and(3±2.6)h separately.Besides both bleeding volume and bleeding time in the Izeatment group were lower than the eonlxol group.Conelusion Guizhi Fuling Capsule plus Mifeoristolle therapy will shorten ovum evacuation time and decrease bleeding volume.

Aged ; Humans ; Lung Diseases, Fungal ; complications ; microbiology ; Male ; Pneumoconiosis ; complications

Stroke is one of the most important causes of disability throughout the world.Epide- miological and clinical studies have demonstrated that statins significantly decrease the incidence of ischemic stroke.In addition to lipid-lowering effect,statins may also have a number of other acting mechanisms that are not dependent on reducing cholesterol levels.Moreover,statins inhibit cerebral vasospasm caused by subarachnoid hemorrhage.For most of the individuals,the safety and tolerability of statins are well.

Objective To demonstrate in vivo that there is different degree myocardium apoptosis during acute ischemia and reperfusion and to explore prelimarily the relations between myocardium apoptosis and the expression of Bcl 2/Bax protein in rats. Methods The morphologic features of apoptotic cardiocytes were pursuited by transmission electron microscopy.DNA extracted from cardiocytes were used to identify the apoptotic DNA ladder by agarose gel eletrophoresis. DNA fragmentation was identified histochemically by TUNEL staining. Expression of Bcl 2/Bax protein in apoptotic myocardium was observed by means of immunohistochemistry and analysed by image analysis system. Results In both ischemia and reperfusion groups morphologic features of apoptotic myocardium were observed by transmission electron microscopic and the apoptotic cardiocytes were detected by the TdT mediated dUTP nick end labeling (TUNEL) staining. However, the apoptotic DNA ladder was observed only in the 2 hour reperfusion group. The reperfusion group showed a decrease in OD value of the Bcl 2 protein( P

Acupressure ; Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Aged, 80 and over ; Combined Modality Therapy ; Female ; Hiccup ; etiology ; therapy ; Humans ; Male ; Middle Aged ; Neoplasms ; complications

Objective To learn the effect of individualized nutritional support in the treatment of severe acute pancreatitis and provide an effective nutritional support therapy for the disease.Methods 97 patients of severe acute pancreatitis were randomly divided into 51 cases of observation group and 46 cases of control group.The control group was given standard parenteral nutrition.The observation group was given the same heat and support stage of individual nutrition nitrogen.Hemoglobin (Hb),albumin (ALB),pre albumin (PA)and transferrin (TFN)of the two groups were detected before and after treatment.The recovery time of serum amylase,resumed oral intake time, hospitalization time and hospitalization expenses of the two groups were observed.The occurrence of gastrointestinal complications was observed in the two groups during the treatment period.Results Compared with before treatment, after treatment Hb,ALB,PA,TFN of the two groups were significantly increased (t =4.114,7.540,5.556,8.307 and 1.994,3.970,2.906,5.099,all P <0.05).After treatment,ALB,PA,TFN of the observation group were higher than the control group (t =2.249,2.256,3.630,all P <0.05).After treatment,recovery time of serum amylase,resumed oral intake time,hospitalization time and hospitalization expenses of the observation group were lower than the control group (t =3.191,5.245,4.846,8.762,all P <0.05).The incidence rate of gastrointestinal complications of the observation group was 11.8%(6 /51),and that of the control group was 15.2%(7 /46),the difference was not statis-tically significant (χ2 =0.248,P >0.05).Conclusion In the treatment of severe acute pancreatitis,individualized nutritional support has better curative effect,low incidence of complications,with good clinical application value.

35 % and the intraoperative blood loss was expected to be 800-1 600 ml. The operations were performed under combined general-epidural anesthesia. General anesthesia was induced with fentanyl 2-4 ?g?kg-1 , thiopentone 5 mg?kg-1 and succinylcholine 1.5mg? kg-1 and maintained with isoflurane inhalation and intermittent iv boluses of vecuronium. Epidural anesthesia was performed at T5-6 (thoracic surgery), T10-11 (upper abdominal surgery) or L3-4 (lower abdominal surgery) . A mixture of 0.1% lidocaine +0.2% dicaine was used for epidural block during operation. The patients were randomly divided into 2 groups: (1) ANIH group ( n = 18) and (2) AHH group ( n = 20) . Blood volume (BV) was calculated according to following formula: BV (ml) (male) = Height (cm)? 28.5 + Body weight (kg) ? 31.6 - 2 820,BV(ml) (female) = Height(cm)? 16.25 + Body weight (kg) ? 38.46 - 1 369. 1 000-1 200 ml of 6% HES (200/0.5) and 500 ml of lactated Ringer's solution (about 25%-30% of BV) were infused at a rate of 50ml?min-1 when induction of anesthesia was started in both groups. In group I (ANIH) 400-600 ml of blood (about 10%-15% of BV) was removed through radial artery before induction of anesthesia. The removed blood was reinfused at the end of surgery. Vital signs (BP,HR,CVP and ST-T changes) , Hct, oncotic pressure and arterial blood lactate concentration were monitored during operation. Results The vital signs were fairly stable during perioperative period in both groups. Transient hypotension developed in 16.7% (3/8 in group Ⅰ ) and 15.0% (3/20 in group Ⅱ ) of patients during induction of anesthesia. CVP was significantly elevated in both groups but the increase in CVP was significantly higher in group Ⅱthan that in group Ⅰ . In group Ⅰ (AN1H) moderate hemodilution was achieved (Hct = 29.9% 2.9%) while in group Ⅱ (AHH) only mild hemodilution was achieved (Hct = 32.9% ?2.9%) .Hct was significantly higher in group Ⅰ(31.5% ?5.1%) than that in group Ⅱ (27.7% ?3.6%) at the end of surgery. Blood loss was comparable between the two groups, and oncotic pressure and blood lactate level were within normal range in both groups. Conclusion ANIH is more effective and safer than AHH without obvious adverse effects, and can avoid exceedingly high CVP commonly seen in AHH. ANIH is a hemodilution technique of choice in the elderly patients.

Objective:To identify the expression of plasmid pLNCX-EGFP-C1-hri targeting the gene of Human ribonuclease inhibitor (hri) in PA317 cells which is capable of expression in mammalian cells.Methods: The vector of pLNCX-EGFP-C1-hri was transfected into PA317 cells by Lipofectamine 2000 and then the expression of recombinated plasmid was verified in living cells by observing the transcription level of egfp-hri fusion gene mRNA with RT-PCR method and the expression level of egfp-fusion hRI protein with western blotting method respectively.Results: Both RT-PCR and western blotting showed the egfp-hri fusion gene was obviously expression in PA317 cells.Conclusion: The plasmid of pLNCX-EGFP-C1-hri targeting hRI is successfully constructed and the protein of hRI can be expressed in PA317 cells correctly.

The study of glomerular mesangial cells of normal rats showed that angiotensin Ⅱ (ATⅡ) down-regulated the expression of MMP-2 mRNA, did not have significant effect on TIMP-2 mRNA. And in consequence, ATⅡ down-regulated the ratio of MMP-2 mRNA to TIMP-2 mRNA.