Acquired Immunodeficiency Syndrome ; diagnosis ; drug therapy ; Adolescent ; Antiretroviral Therapy, Highly Active ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male

OBJECTIVE:To establish a determination method for trace elements of Plumbago zeylanica. METHODS:Induct- ively coupled plasma emission spectrum (ICP-AES) was used to determine seven trace elements of P. zeylanica, i.e. Cu, Zn,Fe,Ca,Mg,Mn and Sr simultaneously. Se was determined by atomic fluorescence (AFS) method. RESULTS: The RSD ranged from 0.78% to 3.01%(n=5),while the recovery was within 91.9%～105.8%. The result obtained was satisfactory. Se took a large proportion in P. zeylanica, followed by Cu,Zn,Fe,Ca,Mg,Mn,Sr.CONCLUSION: The method is brief, rapid and accurate for the determination of P. zeylanica. The study provide reference for future pharmacological study of P. zeylanica.

Excimer laser was found in 1975, and has been used in corneal refractive surgery, which made a great progress and become rapidly developed. ArF laser of 193 nm has high accuracy because it can gasify corneal tissue molecule. Moreover, it does not produce heat effect on tissues surrounding irradiated sites, so not produce adverse effect on corneal structure. At present, excimer laser has been successfully used in corneal radial incision, excimer laser corneal refractive surgery, laser in situ keratomileusis, laser epithelial keratomileusis and laser subepithelial keratomileusis. With increasing understanding of excimer laser and accumulating clinical experience, novel, accurate and predictive excimer laser corneal refractive surgery will be developed.

Delivery of Health Care ; legislation & jurisprudence ; methods ; Female ; Forecasting ; Health Services for the Aged ; legislation & jurisprudence ; supply & distribution ; Humans ; Male ; Medical Informatics ; Prostatic Intraepithelial Neoplasia ; pathology ; Prostatic Neoplasms ; pathology ; Time Factors

China ; Humans ; Immunohistochemistry ; standards ; Pathology, Clinical ; methods ; Quality Control

Objective To sieve the bioactive constituents of Plumbago zeylanica, serum pharmacochemistry research was performed. Method Based on the establishment of HPLC fingerprints of Plumbago zeylanica, the constituents absorbed into blood were determined by comparing the HPLC fingerprints of the methanol extracts, medicated serum samples and blank serum sample. Results One compound absorbed into blood was detected, the other might be metabolites of the original constituent. Conclusion The one onstituent absorbed into blood was possible bioactive component of Plumbago zeylanica.

Objective To analyze and evaluate the role of IABP in the treatment of patients with acute myocardial infarction.Methods The clinical data of all patients with acute myocardial infarction treated with IABP admitted into the Peking University First Hospital from January 1st 2000 to December 31st 2006 were collected.The treatment effects of IABP were analyzed with statistical methods.Results A total number of 48 patients with acute myocardial infarction were treated with IABP in the past 6 years and 26 of them were presented with cardiogenic shock at the time of admission.The short-term condition was stabilized in 81.3%(39/48) of the patients with IABP treatment which was demonstrated by restoration of stable heart rate and blood pressure,increase in urine volume,symptoms relief and decreasing need of vasoactive drug.The in-hospital mortality was lower in patients received revascularization therapy in additional to IABP compared with patients who had IABP support alone(37.5% vs.93.8%,P

Objective To determine the efficacy of stent based rapamycin (Rapa) and methotrexate (MTX) alone or in combination of them to reduce in stent neointimal hyperplasia Rapamycin is a potent immunosuppressive agent that inhibits smooth muscle cell (SMC) proliferation by blocking cell cycle progression Methods Stents were coated with PLGA (poly/lactic co glycolic acid) polymer containing 68-96 ?g Rapa or 250-300 ?g MTX or 58-81 ?g Rapa and 120-170 ?g MTX respectively Twenty five stents (metal, n =8; MTX, n =5; Rapa, n =7; Rapa and MTX, n =5) were implanted in the coronary arteries of 25 pigs Results After 28 days, the mean neointimal thickness was (2 18?1 03) mm 2 in the bare metal stent group; (0 94?0 88) mm 2 in the Rapa group; (0 47?0 24) mm 2 in the combination Rapa and MTX group, (3 93?1 48) mm 2 in the MTX group Compared with metal group the mean neointimal thickness was significantly decresed in Rapa groups and combined group The in stent restenosis was 25% (2/8) in metal group and 80% (4/5) in MTX group after 28 days, and there was no restenosis in the other two groups Conclusion Stent based delivery of Rapa via PLGA polymer can feasibly and effectively reduce in stent neointimal hyperplasia by inhibiting cellular proliferation However there are no effects to reduce in stent neointimal hyperplasia by MTX eluting stents in this study

Objective To investigate the anti-proliferation effect of triptolide-eluting stent, as well as its effectiveress, dose-effect relationship and safety. Methods Experimental stents were divided into 4 groups with 10 stents each, namely bare stent group, sirolimus-coated stent group, 5.6 ?g triptolide-coated stent group and 56 ?g triptolide-coated stent group. By morphometric and histopathologic analysis,we compared 5.6 ?g and 56 ?g triptolide-eluting stents with bare metal stents and sirolimus-eluting stents 28 days after stenting in rabbit iliac models of restenosis. Results The neointimal area of 5.6 ?g triptolide-eluting stents was similar to that of bare metal stents, but larger than that of sirolimus-eluting stents. The neointimal area of 56 ?g triptolide-eluting stents was smaller than that of bare metal stents and 5.6 ?g triptolide-eluting stents, but was similar to that of sirolimus-eluting stents. In all the groups, proliferation on both edges of the stents was insignificant. No toxic effect had been found in the experimental animals related to triptolide-eluting stent. Conclusion Triptolide-eluting stents may inhibit neointimal proliferation. The effect of inhibiting neointimal proliferation is dose dependent and with no adverse effect. These results suggest that triptolide-eluting stent can prevent restenosis within four weeks.

Objective To evaluate the effectiveness and safety of a new designed balloon catheter, the Minirail balloon catheter in coronary intervention Methods Twenty two consecutive patients with coronary artery disease (male 16, female 6, mean age 58 77?8 69 years) were enrolled the study Selective angioplasty was performed for 24 target lesions (17 in LAD, 2 in LCX, and 5 in RCA) Among these target lesions were 5 type C, 14 type B 2, and 5 type B 1 lesions The average extent of narrowing before procedure was 91 18%?5 95% Target lesions were dilated with Minirail balloon catheters Results All of the target lesions were successfully dilated with Minirail balloon catheters The average residual stenosis was 33 18%?25 14% after balloon angioplasty Significant dissection was seen in 3 of the cases, and slow reflow in another one Twenty four stents were implanted in 19 cases The final residual stenosis was 1 82%?5 01% at the end of procedure No death or Q wave myocardial infarction happened No emergency bypass surgery needed Conclusion Minirail balloon catheter is safe, effective, and easy to be used in coronary intervention