AIM: To establish a HPLC method for determing notoginsenoside R_1,ginsenoside Rg_1,ginsenoside Re,ginsenoside Rb_1 in Yunnan Baiyao Powder. METHODS: A RP-HPLC method was applied with a LiChrospher C_(18) column(4.6 mm?250 mm,5 ?m) by gradient elution of acetonitrile-0.4% phosphoric acid solution as the mobile phase.The flow rate was 1 mL/min and the detective wavelength was set at 203 nm.The column temperature was at 30 ℃. RESULTS: All of the four compounds showed good linearities and the recoveries were in the range of 99.6%-101.2%. CONCLUSION: The method is accurate and suitable for the quality control of the product.

Objective:To investigate the effects of all-trans retinoic acid (ATRA) on prolifetation and differentiation in ovarian cancer cell line COC1 in vitro.Methods:Human ovarian cancer cell line COC1 was cultured in medium containing ATRA in different concentrations in vitro.Cell proliferation inhibition was tested by MTT assay.The cell cycle was analyzed by flow cytometry(FCM).Morphological changes of cell differentiation were observed by light and transmission electron microscopy,and the expression of Ki-67 in cancer cells were measured with SP immunohistochemistry.In addition,CA 125 expression in the culture medium was measured by ELISA.Results:ATRA of 10 -7 ～10 -5 mol/L inhibited COC1 cell proliferation significantly in a dose-dependent and time-dependent manner.With the increase of ATRA concentrations,the number of cells in G 0/G 1 phase were elevated,the number of cells in S phase were obviously decreased.COC1 cells after treated by ATRA showed striking morphological characteristics of differentiation.The expression of Ki-67 was decreased in COC1 after ATRA treatment.ATRA could also suppress the expression of CA125 tumor marker in COC1 cells.Conclusion:ATRA can both inhibit proliferation and induce differentiation of human ovarian cancer cell line COC1.These results may provide an effective and novel approach for clinical treatment of human ovarian cancer.

Objective To observe the clinical efficacy of acupuncture in treating functional dyspepsia (FD).Method Eighty FD patients were randomized into a treatment group and a control group, 40 in each group. The treatment group was intervened by needling the specific points of the Stomach Meridian, while the control group was by Itopride hydrochloride tablets. The symptoms score and therapeutic efficacy were evaluated in the two groups.Result There was no significant difference in comparing the symptom score between the two groups before intervention (P>0.05); the symptoms score dropped significantly in both groups after intervention (P＜0.01); the difference in comparing the symptoms score between the two groups was statistically significant after intervention (P＜0.05). The total effective rate was 97.5% in the treatment group versus 67.5% in the control group, and the difference was statistically significant (P＜0.05), indicating that the total effective rate of acupuncture is higher than that of medication in treating FD.Conclusion Acupuncture at the specific points of the Stomach Meridian can obviously reduce FD symptom score, improve symptoms and enhance the total effective rate.

Bronchopulmonary dysplasia is a chronic lung disease of newborn.In recent vears.its incidence is increasing.Bone marrow derived mesenchymal stem cells(BMSC)have been shown to differentiate into a variety of mesenchymal cell types.It has been shown that these cells can be separated.purificated.amplificated and administered to animals and patients in clinical trials.This article focuses on the pathology.pathogenesis of the bmnchopulmonary dysplasia,and biological characteristics of BMSC,and indicates that the cells in the circulation Can engraft in the lung and offer effective protection against lung injuries.

Kawasaki disease is a systemic inflammation of small and middle vessels,especially coronary artery,which is the main cause of acquired heart disease in children in developing countries.Although the rate of coronary artery damage has been reduced significantly after standard treatment,there are 10％ ～20％ cases with no responsiveness to the first dose of intravenous immunoglobulin.This article reviews novel risk factors and treatment advances of intravenous immunoglobulin unresponsiveness in children with Kawasaki disease.

Objective To investigate the significance of plasma B‐type Natriuretic Peptide(BNP) and D‐dimer test in thrombo‐lytic therapy for patients with acute myocardial infraction(AMI) .Methods Determinated the plasma levels of D‐dimer and BNP in patients diagnosed with AMI in the hospital by using full automatic immune fluorescence analyzer on admission (before treatment) , the 7th ,14the and 21th day during treatment ,respectively .Patients were divided into large ,middle and small infarction group ac‐cording to the infarct size determined by color Doppler .The correlation between BNP ,D‐dimer and infarct size were analysed .Re‐sults BNP levels in small ,medium and large infarction group were (165 .0 ± 26 .3) ,(237 .0 ± 39 .4) ,(413 .0 ± 61 .2)pg/mL ,respec‐tively ,and the differences were statistically significant(P<0 .05) .The D‐dimer plasma concentrations in the three groups were all higher than the normal reference level(P<0 .05) .In the course of thrombolytic therapy displayed a dynamic change process ,the level of D‐dimer increased first ,then maintained at a relatively high level ,and finally decreased and returned to the normal level . Conclusion The area of myocardial infarction has a certain correlation with the level of BNP ,and the dynamic monitoring of D‐di‐mer can be used to evaluate the effect of thrombolytic therapy .

Objective To investigate the effect of amplification culture of micro-carrier Vero cells from 30 L bioreactor to 300 L bioreactor after extra-tank trypsinization on the virus-producing ability of rabies virus(RABV)CTN-1Ⅴ strain.Methods The 140-passage of Vero cells were cultured at 37 ℃ for 72-120 h,then amplified by passaging at a cell density ratio of 1∶4 into the 10 × cell factory. After incubation at 37 ℃ for 72-120 h,the monolayer cells were detached and inoculated into the 30 L bioreactor with micro-carriers 7-10 g/L,culture temperature 37 ℃,pH 7. 0-7. 4,dissolved oxygen 30%-80%,stirring speed 10-50 r/min,and continuous perfusion culture 72-120 h. Total three batches of microcarrier Vero cells were cultured,which were amplified to the 300 L bioreactor after extra-tank trypsinization,with microcarrier5-8 g/L,culture temperature 37 ℃,pH 7. 0-7. 4,dissolved oxygen 30%-80%,stirring speed 30-80 r/min,and perfusion culture 72-120 h. RABV CTN-1Ⅴ was inoculated at the MOI of 0. 05,and the virus solution was harvested every 24 h and detected for the virus titer and antigen content. Results The cell density was about 1 × 10~7 cells/mL after culture for 96 h in the 30 L bioreactor,and was about 7. 4 × 10~6 cells/mL after culture for 96 h in the 300 L bioreactor. At 96 h after virus inoculation,the virus harvest solution reached the peak potency,with the average virus titer of 6. 8 lgLD_(50)/mL and the average antigen content of 2. 58 IU/mL. Conclusion The scale-up culture process of micro-carrier Vero cells after extra-tank trypsinization from 30 L bioreactor to 300 L bioreactor is stable and feasible,with no significant effect on the virus-producing ability of RABV CTN-1Ⅴ strain,which provides a reference for the large-scale production of inactivated RABV vaccine

Objective To know the efficacy of oral nursing by visible electric toothbrushes among patients with peroral endotracheal intuabtion. Methods Divided 108 trauma patients with peroral endo-tracheal intubation and clear consciousness into the research group and the control group randomly, there were 54 cases in each group. Routine oral nursing cares were used in the control group, while the visible electric toothbrushes was used in the research group. Compared the efficacy of oral nursing between the two groups. Results The incidence rate of mouth odor, plaque index and oral infection in the research group were better than those of in the control group. Conclusions Using visible electric toothbrushes for trau-ma patients with oral endotracheal tube is a kind of effective and feasibility method, which should be devel-oped in the clinical field.

The development of international academic exchanges has brought a growing de-mand for college English teaching. English for academic purposes (EAP) is playing an increasingly important role in students' study and research. In this essay, a conclusion of college English curricu-lum in the Third Military Medical University was introduced with the theoretic and practical back-ground of oral English for academic purposes (OEAP) teaching. The training strategies of OEAP abilities were discussed from multi-level teaching, personalized teaching and academic forums. The experience of OEAP training was summarized and reflected through teaching practice.

MRI has achieved many technical advances in the spatial resolution, temporal resolution, contrast resolution, signal-to-noise ratio, and postprocessing technique. At one session of examination within a tolerable time, MRI can provide integrated information on coronary artery stenosis, systolic dysfunction, myocardial perfusion, and myocardial viability. Delayed enhancement study after contrast administration is highly reproducible and offers unique vision for myocardial viability in the patients with myocardial infarction. Cardiac MRI is very cost-effective and may be one-stop solution for the evaluation of ischemic heart disease.
Coronary Stenosis ; Humans ; Magnetic Resonance Imaging* ; Myocardial Infarction ; Myocardial Ischemia* ; Perfusion ; Signal-To-Noise Ratio