Adult ; Biomarkers ; blood ; Blood Proteins ; metabolism ; Humans ; Male ; Middle Aged ; Silicosis ; blood

To examine the relationship between genetic variants in the interleukin-6 receptor gene and obesity. The result showed that the carriers of Asp358 homozygotes had higher risk in developing obesity when compared with Ala358 homozygotes (OR = 1.32,95% CI 1.07-1.68, P = 0. 041). Interleukin-6 receptor gene pulymorphism was significantly associated with obesity.

OBJECTIVE:To establish a method for the content determination of sinalbin in Sinapis alba decoction piece. METHODS:HPLC was performed on the column of Sepax HP-C18 with mobile phase of 50% acetonitrile-water solution(contain-ing 5 mmol/L tetraoctyl ammonium bromide)at a flow rate of 1.0 ml/min,detection wavelength was 227 nm,column temperature was 30 ℃,and the injection volume was 10 μl. RESULTS:The linear range of sinalbin was 0.010 1-0.202 4 mg/ml(r=0.999 7);RSDs of precision,stability and reproducibility tests were lower than 2%,recovery was 98.51%-104.62%(RSD=1.90%,n=9). CONCLUSIONS:The method is simple,accurate and reliable,and can be used for determination of sinalbin in Sinapis alba decoc-tion piece.

Objective To know the attitude about palliative care and its influence factors in nurses.Methods Interviewed 8 nurses deeply by qualitative research to know their attitude about pallia-tive care.Results 4 theme,including 9 subtheme were found: nurses' negative emotion caused by job; imperfect of special organization and criterion; characteristics of nursing work.Conclusions Hospital managers should enhance training of nurses' coping capacity with bad feelings, death education and culti-vation of empathy; improve medicare system;develop palliative care routine ; establish palliative care orga-nizations, and relieve nurses' job burden and increase wages.

Objective struclured clinical examination (OSCE) is a kind of examination which could objectively evaluate the students′clinical skills.Nowadays,OSCE are wildly applied in medical educational field throughout the world.Timely discussion and analysis on the problems existed in the implementation process of OSCE is necessary.Measures should be taken to improve the OSCE examination and to meet the requirements of higher clinical practice training level such as increasing clinical skill simulation training hardware investment,optimizing settings and conlents of the examination and the test stations as well as introducing standardized patients (SP) and other measures.

Animals ; Disease Models, Animal ; Facial Nerve Injuries ; etiology ; Female ; Male ; Rabbits

Mian-Zao-Er was collected from the bulbs of Scilla scilloides (Lindl. ) Druce, belonging to the Hyacinthaceae family. 17 compounds were obtained using various column chromatographies on macroporus resin (HPD100), silica gel, Sephadex LH-20 and ODS, as well as semi-preparative HPLC. Their structures were elucidated on the basis of physicochemical properties and spectral data as 2-hydroxy-7-methoxyscillascillin (1), scillascillin (2), 5,7-dihydroxy-3',4'-dimethoxyspiro 2H-1-benzopyran-7'-bicyclo[4.2.0 ] octa [1,3,5 ] -trien } -4-one (3), socialinone (4), 4-methylresveratrol (5), (E)-resveratrol (6), scillavoneA (7), 3,9-di- hydroeucomnalin (8), 3-(3-hydroxy-4-methoxybenzyl) -5,7-dihydroxychroman-4-one (9), (3R)-5,7,3'-trihydroxy-4'-methoxyspiro (2H-1-benzopyran-7'-bicyclo[4, 2, 0] octa [1, 3, 5]-trien} -4-one (10), scillabene A (11), 2-hydroxyscillascillin (12), 3-(4-hydroxybenzyl) -5,7-dihydroxychroman-4-one (13), 3-( 4-hydroxybenzylidene) -5, 7-dihydroxychroman-4-one (14), 3-( 4-hydroxybenzyl) -5-hydroxy-7,8-dimethoxychroman-4-one (15), 3-(4-hydroxybenzyl) -5-hydroxy-6, 7-dimethoxychroman-4-one (16), and 3-(4-hydroxybenzyl)-5,8-hydroxy-7-methoxychroman-4-one (17). Among them, compounds 3, 4, 6, 9, 13 and 15-17 were isolated from this plant for the first time.
Drugs, Chinese Herbal ; chemistry ; Flowers ; chemistry ; Isoflavones ; chemistry ; Mass Spectrometry ; Molecular Structure ; Scilla ; chemistry ; Stilbenes ; chemistry

The genus Scilla consists of 90 species widely distributed in Europe, Asia and Africa, one and its variant of which can be found in China Some species of the genus have been used in traditional medicine to treat various diseases related to inflammation and pain. Phytochemical studies have demonstrated the presence of triterpene and tritepenoid saponins derived from eucosterol, bufadienolides, alkaloids, stilbenoids and lignan in the plants of this genus. Various bioactivities such as antimicrobial, anti-inflammatory, antioxidant, anti-tumor and glycosidase inhibitory activities, have been reported. In this review, the advance of chemical constituents and pharmacological activities of the Scilla species are summarized for further development and utilization of the resource.
Animals ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans ; Scilla ; chemistry

Cerebellum ; surgery ; Cystadenoma, Papillary ; complications ; pathology ; surgery ; Epididymis ; pathology ; surgery ; Follow-Up Studies ; Genital Neoplasms, Male ; complications ; pathology ; surgery ; Humans ; Keratin-7 ; metabolism ; Kidney ; surgery ; Male ; Middle Aged ; Mucin-1 ; metabolism ; Vimentin ; metabolism ; von Hippel-Lindau Disease ; complications ; metabolism ; pathology ; surgery

Objective To investigate the effect of 5-fluorouracil (5-FU) combined with radiotherapy (RT) on the apoptosis of human cervical cancer HeLa cells.Methods The HeLa cells were divided into four groups treated with chemotherapy(ChT),RT,RT+ChT, and control groups.The non-cytotoxic concentration (48 h IC_(50)) of HeLa cells treated with 5-FU was determined by methyl thiazolyl tetrazolium (MTT) and the apoptosis rates of HeLa cells detected by flow cytometry with annexin V-FTTC and PI double labeling.The morphologic changes of the apoptosis cells were observed under fluorescence microsope.Results ChT,RT and RT+ChT treatment could induce the apoptosis of HeLa cells with the strong induction of the combined treatment (P＜0.05).Conclusion 5-FU combined with RT can obviously enhance the apoptosis of HeLa cells, which provides a reliable experimental evidence for clinical use of 5-FU combined with RT in the intervention of human cervical cancer.