Objective To investigate and analyse the status and influential factors of nurses' palliative care knowledge in Shanghai city.Methods Using cluster sampling method,a questionnaire survey using Palliative Care Quiz for Nursing (PCQN) was conducted on 973 nurses in 9 hospitals of Shanghai city.Results 939 nurses completed the survey whose scores were 8.88±2.51.The status quo of palliative care knowledge of Shanghai nurses were not very desirable.The scores of palliative care philosophy and principles and psychological,social and spiritual support were 0.376±0.009 and 0.310±0.008.The scores of knowledge of the pain and symptomcontrol was 0.496±0.005,which was better than the other two aspects.There were differences between dimension 1 and dimension 2,3 (t=-1 1.78,5.465,P＜0.01),and also between dimension 2 and 3 (t=19.673,P＜0.01).Conclusions Lacking of education and training,insufficient recognition of related institutions and the limit of traditional ideas caused the defect in the knowledge structure of palliative nursing.

Objective To investigate the effect of early rehabilitation treatment in the patients with small cerebral hemorrhage and its possible mechanisms .Methods 133 patients with cerebral hemorrhage were randomly divided into the routine treatment , non-early rehabilitation and early rehabilitation groups and given the routine drug treatment .The early rehabilitation group and the non-early rehabilitation group were additionally given the rehabilitative training after 2 d and 14 d respectively .The scores of Fugl-Meyer reassessment and the Barthel index were assessed before training and after 4-week training ,and the serum levels of circulat-ing endotheial progenitor cells(EPCs) and vascular endotheial cell growth factors(VEGF) were detected .Results Compared with the routine treatment group and the non-early rehabilitation group ,the scores of Fugl-Meyer reassessment and the Barthel index af-ter treatment in the early rehabilitation group were significantly increased (P< 0 .01) .The serum levels of circulating EPCs and VEGF in the early rehabilitation group were also significantly increased compared with the routine treatment group control (P<0 .01) .Conclusion Early rehabilitation treatment can obviously improve the limb movement function and the daily living ability in the patients with small cerebral hemorrhage ,its mechanisms may be involved with the increase of circulating EPCs and VEGF .

Objective: To examine and analyze the expression of two isoforms of livin (livin α/livin β), a novel inhibitor of apoptosis protein (IAP) family member, in tumor cell lines and malignant tumor tissues and analyze the relationship between livin expression and clinical pathological features of different tumors. Methods: Livin mBNA expression was detected by reverse transcription polymerase chain reaction (BT-PCR) in 12 malignant tumor cell lines, 50 different malignant tumor tissues, and 20 cases of normal tissues. Results: Livin mBNA was highly expressed in seven cell lines (Hela, SPEA-1, SBE-2, PC14, MKN45, LOVO, and HHCC) and poor expressed in A549 cells, It had negative expression in other four kinds of cell lines ( HL-7702, Hep-2, 7721 and K562). Livin mRNA was positively expressed in 32 of 50 malignant tumor cases (64.0%). The expression of livin mRNA was at low level in paracancerous tumors tissues (2%). Livin expression had no significant association with differentiation degree, lymph node metastasis and TNM staging of tumor patients. Conclusion: Livin expression in tumor cell lines and malignant tumor tissues provides stronger evidence for further investigating the relationship between livin and tumor initiation and development. It may serve as a new target for malignant tumor diagnosis and therapy.

AIM: To construct the eukaryotic expression vector CD80-IgG by fusing the cDNA encoding extracellular portion of murine CD80 to the 5'-terminus of cDNA encoding Fc fragment of murine immunoglobulin G1 and to express the fusion protein in Chinese hamster ovary (CHO) cells. METHODS: The two cDNAs was amplified by PCR respectively from plasmid pcDNA/B7 containing the full-length cDNA of murine CD80 from murine spleen cells, and cloned to the eukaryotic expression vector pcDNA3.0 by directional cloning. The resultant recombinant plasmid pcDNA/CD80-IgG was transfected into CHO cells with liposome transfection reagent. The stably expressing cells were obtained by G418 screening. Western blot, Dot ELISA, and flow cytometry were used to detect the expression of the fusion protein and its immunological activity. RESULTS: DNA sequencing verified the correction of the construction of recombinant plasmid pcDNA/CD80-IgG. The expressed fusion protein was detected in the supernatant of transfected CHO cells and the molecular weight of the protein was similar to what we expected. Its immunological activity was also established. CONCLUSION: The recombinant plasmid pcDNA/CD80-IgG was successfully constructed and it expressed the fusion protein CD80-IgG.

Humans ; Tuberculosis, Laryngeal

Objective In order to explore the relationship between the “Wits" appraisal and the ANB angle, and to see how accurately one can predict the “Wits”. Methods Given the ANB, lateral cephalometric radiographs were taken on 50 boys and 50 girls between the ages of 11～13. Results Each subject had no treatment either a Class Ⅰ or Class Ⅱ malocclusion, a good growth pattern. Group Ⅰ , 13 subjects of 100 had ANB≤1.5°, and negative “Wits” appraisal; group Ⅱ , 61 subjects had 5.8°＞ANB＞1.5°, and “Wits” appraisal either positive or negative; group Ⅲ , 26 subjects had ANB angle≥5.8°, and positive “Wits” appraisal. Statistically analysis showed that there was no sig nificant difference in sex (P＞0.05); there was significant correlation between the ANB and “Wits” values in the group Ⅱ and group Ⅲ (P ＜0. 05); there was statistical significance in regression analysis in the group Ⅰ and group Ⅱ (P ＜0. 05). We could predict the “Wits” with 21％ accuracy in group Ⅱ. Conclusion The results indicate that these figures are statistically significant but clinically irrelevant.

Objective To compare the effect of etomidate and propofol on cardiac and renal function in perioperative elderly patients. Methods 120 perioperative elderly patients were selected and divided into two groups.60 cases in etomidate group (T group) were anesthesia induced by etomidate, and 60 cases in propofol group ( B group) were anesthesia induced by propofol.Hemodynamics, renal function and perioperative indexes were compared after operation.Results Compared with before induction, SBP, DBP and HR were lower after induction(P<0.05), and compared with T group at the same time, SBP, DBP and HR of B group were lower( P<0.05).Compared with before induction, SI, CI and EV were lower after induction (P<0.05), and compared with T group at the same time, SI, CI and EV of the B group were lower(P<0.05).Compared with B group, Cr, BUN and β2-MG of T group were lower ( P<0.05 ) .Conclusion The effects of etomidate on inhibition of cardiovascular system and damage of renal function is slighter, which has important significance for elderly cancer patients.

Cells, Cultured ; Humans ; Neovascularization, Physiologic ; immunology ; Pericytes ; metabolism ; physiology

OBJECTIVE:To observe clinical efficacy and safety of triptorelin acetate in the treatment of endometriosis after lap-aroscopy. METHODS:196 cases of endometriosis were randomly divided into experimental group and control group with 98 cases in each group according to admission sequence. Both groups received laparoscopic surgery. After surgery,experimental group was given triptorelin acetate by intramuscular injection,3.75 mg/time,every 4 weeks;control group was given gestrinone orally,2.5 mg/time,twice a week,for 5 months. The ovulation,menstrual recovery and the occurrence of ADR were observed in 2 groups. The endometriosis relapse of 2 groups were followed up for 1 year and observed. RESULTS:After treatment,the ovulation recov-ery time and menstrual recovery time of experimental group were all shorter than those of control group,with statistical signifi-cance(P<0.05). The incidence of ADR in experimental group(17.34%)was significantly lower than in control group(35.71%), with statistical significance(P<0.05). During follow-up period,the relapse rate of experimental group(7.14%)was significantly lower than that of control group(18.37%),with statistical significance(P<0.05). CONCLUSIONS:After laparoscopic surgery, triptorelin acetate can significantly improve therapeutic efficacy of endometriosis,reduce the occurrence of ADR.