Objective]The article summarizes in diagnosis and treatment of dysmenorrhea academic thoughts and characteristics of drug use. [Methods] Summary by readingtext and access to a large number of relevant literature,the history of famous experts,combined with the actual clinical, summarized his main syndrome differentiation and treatment of dysmenorrhea thought and medication. [Results] Fu Qingzhu according to the onset of time divided into 5 types of dysmenorrhea, menstruation comes or breaks suddenly when pain exists or disappears, the menstruation upcoming accompanies abdomen ache, abdominal pain after menstruation,the menstruation upcoming lower abdominal pain and hematemesis and the umbilicus pain when the menstruation upcoming.Fu Qingzhu thought according to the pathogenesis of dysmenorrhea mainly with the liver, spleen and kidney three organs close relationship, the syndrome is stagnation of the liver qi and blood deficiency, liver depression of the fire, kidney deficiency and liver qi stagnation and reversal of liver qi, deficiency of spleen Yang, treatment with soothing liver and reinforcing kidney mainly.[Conclusion] Fu Qingzhu ’s idea in the dysmenorrhea was analyzed in detail, and pointed out the mistakes in the syndrome differentiation and put forward the original opinion and treatment of medicine, in order to inherit traditional Chinese medicine academic, improve the curative effect of dysmenorrhea.

Chlorella pyrenoidosa No.2 was screened from five species of microalga Chlorella sp. for its higher lipid yield. Effects of medium components and culture conditions on cell growth as well as lipid ac-cumulation of C. pyrenoidosa No.2 were investigated and the results showed that the optimum medium rec-ipe was 20.0 g/L glucose,0.08 g/L glycine,1.0 g/L K2HPO4?3H2O,0.4 g/L MgSO4?7H2O and 0.004 g/L FeSO4?7H2O. The optimum culture temperature,initial pH,shaking rate and light intensity were 28℃,6.0,130 r/min and 650 Lux,respectively. Biomass and lipid content increased from 3.73 g/L and 40.15% to 6.56 g/L and 59.90% when Chlorella pyrenoidosa No.2 was cultivated under the above optimal conditions for 7 days,with lipid yield raised by 162%. Chlorella pyrenoidosa No.2 could produce lipid with xylose as carbon source,and so is potential for lipid production from renewable materials such as lignocellulose. GC analysis demonstrated that the fatty acid composition of the lipid was similar to that of vegetable oil and its unsaturated fatty acid content reached around 71%,thus it is a promising material for biodiesel production.

Objective: To apply the NRS2002 to screen the nutritional status of preoperative patients and investigate the nutrition support in the perioperation and clinical outcomes. Methods: 127 selective operational cases(including general surgery,thoracic surgery,gynecology and orthopedic) were recruited to adopte the NRS2002 which issued by CESPN in 2006,and the nutrition support,energy and nutriment in the perioperation,complications,length of stay and drug costs were investigated. Result: 30.7% patients needed nutrition support,with general surgery(28.3%) being higher than thoracic surgery(2.4 %),gynecology(0%) and orthopedic(0%).The nutritional risk in elderly,carcinoma,abdominal operation patients were 18.1%,19.7% and 18.1% seperately,which was higher than others(P

Aim To study the effect of ZD7288 on synaptic transmission in the pathway from perforant pathway (PP) fibers to CA3 region in rat hippocampus. Methods The extracellular recording technique in vivo was used to record the CA3 region field potentials. High-performance liquid chromatography (HPLC) with fluorescence detection was applied to measure the content of amino acids in hippocampal tissues. The effect of ZD7288 and CsCl on the amplitudes of population spike (PS) in CA3 region evoked by stimulation (0.5 Hz) of the perforant pathway (PP) fibers, and the content of amino acids in hippocampal tissue were observed. Results Microinjection of ZD7288 (20, 100 and 200 nmol)and CsCl (1,5 and 10 μmol) into CA3 region decreased the population spike (PS) amplitudes in a dosedependent manner. The inhibitory effects appeared at 5 min after microinjection and lasted at least 90 min.In those rats treated with ZD7288 (100 nmol), the contents of glutamate, aspartate, glycine and GABA decreased significantly as compared to those of saline control ( all P＜0.01, except P＜0.05 for that of glycine). A similar decrease in the contents of amino acids was observed when the rats were microinjected with CsCl (5 μmol). Conclusion ZD7288 could obviously inhibit synaptic transmission in the pathway from PP fibers to CA3 region in rat hippocampus, and this action of ZD7288 may be associated with altered contents of amino acids.

Adenocarcinoma ; metabolism ; pathology ; Adenocarcinoma, Clear Cell ; metabolism ; pathology ; Biomarkers ; metabolism ; Carcinoma ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Female ; Humans ; Keratins ; metabolism ; Membrane Proteins ; metabolism ; Middle Aged ; Mucin-1 ; metabolism ; Myoepithelioma ; metabolism ; pathology ; surgery ; Neoplasm Recurrence, Local ; Parotid Gland ; surgery ; Parotid Neoplasms ; metabolism ; pathology ; surgery ; S100 Proteins ; metabolism

This research is to investigate study the flavonoids from stems of Nelumbo nucifera and the cytotoxic activities of iso- lated compounds. The constituents were separated by column chromatography,and their structures were elucidated by spectroscopic data analyses. The isolated compounds were evaluated for cytoxic activities by MTT method. Twelve compounds were isolated and identified as rhamnazin-3-O-beta-D-glucopyranoside (1), luteolin-3', 4'-dimethylether-7-O-beta-D-glucoside (2), kaempferol-3-O-beta-D-xylopyranosyl-(1-->2)-O-beta-D-glucopyranoside (3), quercetin-3,3'-di-O-beta-D-glucopyranoside (4), 1, 8-dihydroxy-3,7-dimethoxyxanthone (5), isorhamnetin-3-O-beta-D-glucopyranoside(6) , kaempferol(7), isorhamnetin (8), quercetin(9), astragalin(10), hyperoside (11) and 1-hy- droxy-3,7,8-trimethoxyxanthone(12). All compounds were isolated from stems of this plant for the first time, and compounds 1-5 were firstly isolated from the family nelumbonaceae. Compounds 24 and 6 showed significant cytotoxic activities against BEL-7402 carcinoma cell lines at a concentration of 1 x 10(-5) mol x L(-1) with the inhibitory rate of 67.36%, 53.25%, 57.78%, 60.13% and 52.11%, respectively.
Cell Line, Tumor ; Flavonoids ; chemistry ; pharmacology ; Humans ; Nelumbo ; chemistry ; Plant Extracts ; chemistry ; pharmacology ; Plant Stems ; chemistry

ObjectiveTo evaluate the usefulness of an ultrasonography-based method to examine quadriceps femoris contracting. MethodsThe thickness of bilateral rectus femoris (RF) and vastus intermedius (VI) and the cross-sectional area (CSA) of RF was measured in 10 healthy subjects （n=20） in a relaxed position and 3 isometric contraction states using ultrasonography by two examiners. The date was compared with paired t test. Half of these subjects （n=10） were also measured with MRI at rest. The date was also compared with paired t test. ResultsThere was no significant difference between MRI and static compound ultrasonography, nor between two examiners. There was a significant difference (P<0.05) in thickness of RF and VI between relaxed and isometric contraction conditions. There was no significant difference among isometric contraction conditions. There was no significant difference among measurements of RF-CSA. Conclusionultrasonography shows good validity and reliability in measuring quadriceps shape.

Objective To evaluate the teaching effects of micro-lecture based on the wechat platform in new nurses'training in operating rooms.Methods Fifteen new nurses in the operating room in July 2014 were selected as observation group,and thirteen new nurses in July 2013 were selected as control group.The multimedia-aided teaching mode was applied in the two groups,while micro-lecture based on the we-chat platform was implemented in the observation group.The theory and operating skills between two groups were evaluated by examination, and a random inquiry about satisfaction with new nurses was directed on surgeon and seniors nurses.Resulsts 100% of the nurses in obser-vation group accepted and preferred wechat in continuing education.The new nurses’performance in theory and skill test in the observation group was significantly higher than that of the control group (t =2.901,P =0.011;t =2.225,P =0.029).In addition,the surgeon’s and seniors nurses’satisfaction with new nurses in the observation group was significantly higher than that of the control group (Z =6.425,P =0.011).Conclusion Micro-lecture based on the wechat platform can motivate nurses’learning enthusiasm and positivity,and effectively improve their theoretical knowledge and professional skills.

Enzymatic hydrolysis of nitrile was performed under mild conditions and afford high efficiency and selectivity,thus being highly potential for the synthesis of optically active carboxylic acids and their derivatives.The type of nitrile-hydrolyzing enzymes and hydrolysis reactions,reaction characteristics,factors influencing the hydrolysis and the prospect for its application to industrial production were reviewed in this paper.

Objectives:To observe the relationship between 2 different ways of nasal tube nutrition supplement patterns and clinical patients'endurance and complication. Methods:Self contrast study was used in 8 cases of old patients supplied with nasal tube nutrition for 4 months(perfusion and dripping pattern 2 months each). Biochemical indices and enteric complications before and after nasal tube nutrition were observed. Results:Slowly dripping supplement pattern greatly decreased the incidence of inhalation pneumonia and other complications related with gastro enteric nutrition supplement,such as diarrhea,bloating,vomits and etc.The nutritional status was obviously improved when the continuous dripping supplement pattern was used. Conclusions:Continuous dripping supplement pattern is better than perfusion supplement pattern.