Objective:To study the effects of nimesulide on proliferation and apoptosis of human gastric carcinoma cells SGC-7901 in vitro,and provide an experimental basis for clinical application.Methods:Proliferation of human gastric carcinoma cells SGC-7901 was assessed by MTT,and apoptosis was detected by transmission electron microscope and flow cytometry.Results:Proliferation of SGC-7901 was inhibited significantly by nimesulide(range from 12.5 to 800?mol/L) in a dosage-dependant manner ( P

The medical care reform is at the stage of rapid development of economic,science and technology in China. Facing the problems and the reform trend of focusing on the hospitals at basic level for medical services,medical schools should play the role in training medical personnel for community and township. The pathways for cultivating the applicable medical talents include the scale enlargement and structure adjustment,quality control,curriculum reform,postgraduate education and training program on post.

Problem-based Learning (PBL) has become a curriculum model in medical education. The paper expounds its emergence and development, concept, characteristics of learning, fundamental teaching process, problem designing, advantages and disadvantages, and features of curriculum contents.

Pharmacognosy (Chinese Medicine) discipline has a long history and International cooperation is more and more frequent, but the communication of the Chinese traditional medicine cul-ture is very difficult. In order to solve this problem, it is urgent to improve the professional English level of the students. Thus, combined with the education survey and interview results, as well as the reports in previous studies and our own experience, we think about how to set pharmaceutical profes-sional English course and put forward some suggestions such as the innovation of curriculum organiza-tion strategy, convergence of basic English course, teaching content and form being closely linked to academic activities, and paying attention to highlighting the subject characteristics of pharmacognosy (Pharmacy).

Objective To establish a RP-HPLC method to determine the content of geniposide in Xiaoyan Tuire Patch. Method The column of Agilent ZORBAX SB-C18 (4.6 mm?250 mm 5 ?m) was used. The mobile phase was composed of water-acetonitrile-phosphoric acid (86∶14∶0.01). The flow rate was 1 mL/min, with UV detection at 238 nm, and the temperature was 30 ℃. Results The linear range was 0.07～0.70 ?g (r =0.99997). The average recovery was 98.60% and the RSD was 1.96%. Conclusion The method is simple, sensitive and accurate, and can be used for the determination of Xiaoyan Tuire Patch.

Objective To establish a RP-HPLC method to determine the content of ephedrine?HCl in Xiaoyan Zhike Plaster. Method The sample was prepared according to 2005 edition of Chinese Pharmacopocia, the column of Inertsil-3 ODS (4.6 mm?250 mm, 5 ?m) was used. The mobile phase was composed of methanol-0.1% of phosphoric acid (5∶95). The flow rate was 1 mL/min, with UV detection at 207 nm. Results The linear range was 0.041～0.41 ?g (r=0.999 8). The lowest limit of detection was 0.025 6 ?g. The average recovery and the precision was 99.55% and 2.58%. Conclusion The method is simple, sensitive and accurate, and can be used for the quality control of Xiaoyan Zhike Plaster.

AIM:To investigate the feasibility and specificity of gastric carcinoma gene therapy by utilizing RNA interference (RNAi) to inhibit survivin expression in vitro and in vivo. METHODS:Small interference RNA (siRNA) homologous to survivin was designed. pTZU6+1-siRNA-survivin vector was constructed and transfected into BGC-823 cells. The transplanted BGC-823 tumor in nude mice was established to induce RNAi. The changes of survivin gene expression,tumor cell cycle and cell apoptosis were detected by flow cytometry,RT-PCR,Western blotting,immunochemistry and TUNEL. RESULTS:The expression of survivin was obviously inhibited by RNAi in vitro. The phase of cell cycle indicated the reduction of S phase,while G1/G0 phase increased. Cell apoptosis was obvious. Both the mRNA level and the protein expression of survivin decreased obviously. The tumor size reduced after treated with pTZU6+1-siRNA-survivin vector in vivo. The expression of survivin decreased in siRNA treatment group. In contrast,little change in control group in vitro and in vivo was observed. CONCLUSION:RNA interference down-regulates survivin gene expression,inhibits BGC-823 cell proliferation and induces cell apoptosis with good specificity,which may be a possible new approach for neoplasm gene therapy.

Objective To investigate whether insulin resistance is a cause of pregnancy induced hypertension (PIH). Methods All patients who had abnormal 50g glucose screening test accepted 75g oral glucose tolerance test (OGTT) and insulin sensitive index (ISI) was calculated. These patients were followed up and divided into two groups (PIH group and normal pregnancy group) at third trimester of pregnancy. The difference of ISI between the PIH group and normal pregnancy group was compared. Results Fast blood glucose, fast blood insulin and ISI were (4.2?0.7)mmol/L vs. (3.8?0.7)mmol/L, (107.8?48.8)pmol/L vs. (50.4?40.5)pmol/L, and -3.25?0.27 vs. -2.58?0.66 in PIH group and normal group, respectively ( P 0.05). Conclusions Insulin resistance during second trimester of pregnancy may be one of the causes of pregnancy induced hypertension.

Traditional Chinese medicine (TCM) contains a big array of chemical constituents. These constituents un-dergo various metabolic reactions to produce complex in v iv o metabolites. Identification and distribution of these metabolites are important to reveal the effective constituents from a complex formulation. Recently, developments had been made to elucidate the complex metabolic process. In this review, we summarized the methods and strategies developed for TCM metabolism. Application of these metabolic results has also been discussed.

We took such measures in internal medicine practice teaching in five-year program as actualizing special teachers’supervision system,adopting multiple teaching methods (enlightening,case-based,question-based,multimedia),taking interview of teachers and students,and enhancing doctor-patient communication.