Objective:To explore therapeutic effect of coronary artery stent implantation (CASI) on acute myocardial infarction (AMI ) . Methods :According to voluntary choice , a total of 89 AMI patients were divided into drug thrombolysis group (n=44 ,received intravenous drug thrombolytic therapy) and CASI group (n=45 ,received CA‐SI treatment) .Therapeutic effect ,symptom relieving time after treatment ,hospitalization duration and left ventric‐ular ejection fraction (LVEF) were compared between two groups ;all patients were followed up for 1~3 years ,in‐cidence of death and cardiac events ,including re -occlusion ,severe heart failure and severe arrhythmias were ob‐served and compared between two groups .Results:Success rate of reperfusion of CASI group was significantly high‐er than that of drug thrombolysis group (91.11% vs .68.18% ) , P<0.05. Compared with drug thrombolysis group , there were significant rise in LVEF [(42.58 ± 13.10)% vs .(57.32 ± 18.61)% ] ,and significant reductions in symp‐tom relieving time [ (8.09 ± 8.31) h vs .(3.75 ± 3.58) h] and hospitalization duration [ (19.84 ± 8.15) d vs . (13.01 ± 5.40) d] in CASI group , P<0.01 all .During follow -up ,compared with drug thrombolysis group ,there were significant reductions in incidence rates of severe heart failure (18.18% vs .4.44% ) ,severe arrhythmias (22.73% vs .6.67% ) and death (6.82% vs .2.22% ) in CASI group , P<0.05 or <0.01.Conclusion:Coronary ar‐tery stent implantation possesses significant therapeutic effect on acute myocardial infarction ,it can significantly shorten hospitalization duration ,and its incidence rate of adverse cardiac events is significantly lower than that of drug thrombolysis ,which is worth extending .

Objective To investigate the chemical constituents in the whole plant of Clerodendrum canescens. Methods The compounds were isolated by column chromatography over silica gel or Sephadex LH-20 gel and their structures were elucidated by chemical and spectroscopic methods. Results Seven triterpenoids were isolated and identified as: lupeol (Ⅰ), ?-amyrin 3-undecanotate (Ⅱ), lupeol acetate (Ⅲ), lupeol 3-palmitate (Ⅳ), melastomic acid (Ⅴ), ?-amyrin acetate (Ⅵ), betulinic acid (Ⅶ), respectively. Conclusion Among those compounds, compound Ⅱ is a new one, while compounds Ⅳ-Ⅵ are isolated from the plants of Clerodendrum Linn. for the first time.

OBJECTIVE:To investigate the utilization of antibiotics in urinary surgery patients in our hospital.METHODS:The problems lie in the perioperative use of antibiotics in a total of 600 patients with ureter stones,kidney stones,bladder stones and(or)benign prostatic hyperplasia(BPH)undergoing urinary surgery from Jan.to June in 2006 and from Jan.to June in 2007 were analyzed in accordance with the Guiding Principles of Clinical Application of Antibacterials and the related regulations of antibiotics in our hospital.RESULTS:The rate of rational use of antibiotics rose from 12.5% before intervention to 88.2% after intervention.The problems of improper selection of antibiotics,irrational timing and too long duration of drug use were improved to a great extent.CONCLUSION:The intervention on the rational perioperative use of antibiotics is effective and it greatly enhanced the rationality in the perioperative use of antibiotics in urinary surgery patients.

The Wnt signaling pathway,including the canonical and the non-canonical,is a conservative pathway which participates in organ development and tissue metabolism.The canonical Wnt pathway plays an important role in osteogenesis,bone formation and mineralization through the osteogenesis associated transcription factors.The canonical Wnt pathway also regulates osteogenic differentiation of the stem cells in the periodontal tissue and the regulative effect is influenced by the microenvironment.Understanding of the canonical Wnt pathway will contribute to the treatment of some related bone diseases.

ObjectiveTo investigate the application and effect of problem-based learning ( Problem-based learning,PBL ) pedagogy in theoretical teaching of pediatric nursing.MethodsThe two nursing classes in the same grade were selected respectively as a control group of 62 people and research group of 62 people.Traditional pedagogy was taken in control group to give lessons for theory pediatric nursing,while PBL pedagogy was taken in research group to give lessons ftr theory pediatric nursing.At the end of the semester,students' scores in theory exam,practice exam and case care were analyzed by using t tests.ResultsThe results of the two groups of students were compared,with t value 4.96 of the theoretical results,t value 5.312 of the experimental results,t value 8.06 of the case study,and P ＜0.05 in three items,suggesting statistically significant difference.ConclusionsPBL pedagogy can raise nursing students' learning interest in the theory of pediatric nursing,and improve teaching effect on theory of pediatric nursing.

Objective To investigate the effects of macmphage migration inhibitory factor (MIF) on glucocorticoid (GC) re]ease and glucocorticoid recer (GR) in mts.Methods Test Ⅰ Thirty-two male SD rats weighing 250-300 g were randomly divided into 4 groups(n=8 each):control group(C),low dose recombinant MIF (rMIF) group (rMIF-L),middle dose rMIF group (rMIF-M) and high dose rMIF group (rMIF-H).The animals received l ml normal saline via the right femoral vein in group C.The animals received rMIF50.100 and 200 ng in l ml normal saline though right femoral vein in group rMIF-L,rMIF-M or rMIF-H respectively.Blood samples were taken from left femoral artery immediately before inection(T0,baseline),and at 5 min,3 h,6 h.12 h and 24 h after injection of rMIF(T1-5) for determination of serum concentration of corticosterone.Test Ⅱ Primary cultured neonate rat(2-3 days)myocardial ceils were randomly divided into 3 groups(n=24 each):group C,group rMIF-L and group rMIF-M.The ceUs in group C,rMIF-L and rMIF-M wefe incubated with DMEM.rMIF 50 ng+DMEM and rMIF 100 ng+DMEM for 3 h respectively.The expression of GR and HsPg0 wag determined by Western blot.ResuBs Test Ⅰ The serum concentration of corticosterone was signifieemily higher in the other 3 groups than in group C at T1-5(P<0.05).The sertlm concentration of corticostemne was significantly increased at T1-5 in group rMIF-L,rMIF-M and rMIF-H compared with the baseline values(P<0.05).Test Ⅱ HSP90 expresion was significantly lower in the other two groups than in group C(P<0.05).Them was rio signifieanf difference in HSP90 expression between group rMIF-L and group rMIF-M(P>0.05).There was no significant difference in GR expression among the 3 groups ( P > 0.05). Conclusion MIF druing sepsis can weaken GR function through down-regulating HSP9O expression, resulting in CC resistance.

This paper reports the diagnosis and treatment of one rare case of thyroid dysfunction caused by Strongyloides ster-coralis infection.

Mesenchymal stem cell (MSC) resides in a relatively hypoxia niche (volume fraction 1％～7％)compared to normoxia (20％).MSC cultured in hypoxia condition shows different biological features from those in normoxia condition.Hypoxia inducible factor-1 α (HIF-1 α),as one of main transcription factors in hypoxia,participates in the regulation of cell proliferation,differentiation,apoptosis,glycolysis and angiogenesis.Recent researches show that HIF-lα also plays an important role in regulating the survival,differentiation and migration of MSC.It turns out that hypoxia can improve survival and migration of MSC,enhance chondrogenesis but inhibit adipogenesis through HIF-lα and its downstream genes.However,whether hypoxia promotes or impedes osteogenesis remains controversial.Such differences can be caused by variances of tissue origination,oxygen concentration and culture condition.The comparison of different researches should be based on the same experiment condition.This review focuses on the influence of hypoxia on proliferation,apoptosis,differentiation and migration of MSC.

Objective To explore the clinical characteristics of thyroid disease in patients with primary Sj(o)gren's syndrome (pSS).Methods A total of 81 pSS patients were retrospectively investigated for thyroid dysfunction and the presence of anti-thyroid peroxidase antibody (TPOAb),antithyroglobulin antibody (TGAb).Sex,age,erythrocyte sedimentation rate (ESR),C-reactive protein (CRP),white blood cell count,and serum immunoglobulins were also analyzed.Results Thirty-one patients were identified as having thyroid dysfunction,including 3 with hyperthyroidism,18 with hypothyroidism,and 10 with subclinical hypothyroidism.There were no significant differences in the incidences of some clinical manifestations between patients with or without thyroid dysfunction,such as dry mouth,dry eyes,joint pain,Raynaud's phenomenon,palpable purpura,peripheral neuropathy,interstitial lung disease,liver involvement,and renal tubular acidosis.Differences were not statistically significant for ESR and CRP between two groups.Also,the titers of anti-nuclear antibody,anti-SSA antibody,antiSSB antibodies,and rheumatoid factor between two groups showed no significant differences.However,white blood cell count [(5.65 ± 0.49 vs 7.22 ± 0.48) × 109/L,P＜0.05] and hematoglobin [(110.50 ± 3.64 vs 122.80 ± 3.02) g/L,P＜0.05],as well as the titers of TGAb [(587.30 ± 204.30 vs 58.76 ± 13.78) U/ml,P＜0.05] and TPOAb [(213.70 ± 39.64 vs 29.57 ± 8.49) IU/ml,P ＜ 0.05] were significantly different between the two groups.Conclusions Thyroid diseases are frequently associated with pSS.Therefore,extended screening for existing thyroid diseases during the routine assessment of pSS patients is recommended.

Objective To investigate the elimination effect of propofol on the reactive oxygen species(ROS) in patients with acute craniocerebral injury (ACI). Methods Forty patients with ACI were randomly divided into propofol group receiving propofol anesthesia and ?-OH group as control . Electron spin resonance(ESR) spectroscopy was used to determine the plasma contents of oxygen free radical (OFR), and the plasma contents of lipid peroxides(LPO) and nitrogen oxide (NO) were determined with chemical method. Blood samples were collected before anesthesia and 2, 4 hours after starting operation. Results The plasma contents of NO, OFR and LPO significantly increased before operation in patients with ACI compared with healthy subjects(all P