Oxidative stress played an important role in the development of diabetes and its complications. Nuclear factor erythroid 2-related factor 2 (NRF2) pathway is one of the most vital endogenous antioxidant pathways. Accumulated evidences indicated that the relationship between diabetes and NRF2 pathway attracted more and more attention in recent years. Our group has devoted ourselves to the researches concerning the chronic complications of diabetes and NRF2 pathways. This review highlighted our recent progresses in the underlying mechanism of the protective role of NRF2 in diabetic nephropathy, diabetic ulcers and diabetic amyotrophy. Finally, the possibility of NRF2 agonists applied to clinical therapy for diabetic chronic complications was explored.

Aim To study the effects of preconditioning with desflurane, sevoflurane and isoflurane on adenosine triphosphate (ATP) in anoxia-reoxygenation myocardial cells. Methods Rat ventricular myocytes, cultured for 4～5 days, were randomly allocated to five groups: Control group, anoxia-reoxygenation group and groups preconditioned with 1.5 MAC desflurane, sevoflurane or isoflurane following anoxia-reoxygenation. The content of intracellular ATP ,the activities of lactic dehydrogenase(LDH) and creatine kinase(CK), and the cell viability were measured at the end of experiment.Results Preconditioning with 1.5 MAC desfllurane, sevoflurane or isoflurane significantly attenuated the great reduction in ATP and cell viability and the increase of LDH and CK caused by anoxia-reoxygenation. There was a positive correlationship between ATP and cell viability,and a negatiue correlationship between LDH and CK (r was 0.83, -0.87 and -0.82 respectively, P

ObjectiveTo investigate the effect of mild hypothermia on neuron specific enolase (NSE) and superoxide dismutase (SOD) in serum of patients with large-area cerebral infarction.Methods160 cases with large-area cerebral infarction were divided into the treatment group and control group with 80 cases in each group. Patients of the control group were treated with routine therapy. Those of the treatment group were added with mild hypothermia therapy (MHT). The scores of NIHSS were assessed and NSE and SOD in serum were examined before treatment and 7, 14 and 30 days after treatment in two groups.ResultsThe NIHSS score of the treatment group was significantly lower than that of the control group ( P<0.05), and NSE level decreased, SOD vitality increased in the treatment group. Other indexes such as respiratory, pulse, serum kalium and etc of two groups were not different ( P>0.05).ConclusionMHT can improve he nerve function of patients with large-area cerebral infarction recovering and improve prognosis.

Humans ; Inflammation ; drug therapy ; metabolism ; MicroRNAs ; metabolism ; Stilbenes ; pharmacology ; therapeutic use

Angioplasty, Balloon, Coronary ; instrumentation ; Female ; Humans ; Middle Aged ; Prosthesis Failure ; Stents

Objective To explore the correlation between serum level of cystatin C and coronary slow flow (CSF).Methods Thirty-four patients with CSF were enrolled in CSF group and thirty-five patients with normal coronary flow and angiographically normal coronary arteries were enrolled in control group.Coronary flow patterns was assessed by corrected thrombolysis in myocardial infarction (TIMI) frame count.The change of serum high sensitivity C-reactive protein,uric acid,cystatin C were measured.Results There was no significant difference between two groups with respect to gender,age,history of smoking,prevalence of hypertension and diabetes mellitus,family history of coronary heart disease,low density lipoprotein,α-lipoprotein (P ＞0.05).Compared with control group,the level of serum high sensitivity C-reactive protein,cystatin C,uric acid in CSF group were obviously higher [(4.85 ± 6.39) mg/L vs.(2.55 ± 2.18) mg/L,(0.87 ± 0.22) mg/L vs.(0.75 ± 0.16) mg/L,(329.68 ± 85.46) μ mol/L vs.(278.97 ± 76.74) μ mol/L] (P ＜ 0.05 or ＜ 0.01).Logistic regression analysis showed that cystatin C increased as independent risk factors for CSF (P =0.002,OR =0.009).Conclusion High level of cystatin C may play an important role in the occurrence and development of CSF.

BACKGROUND:There is a close connection between the occurrence and development of tumor stem cels and ovarian cancer. CD90+ is an important tumor stem cel marker.
OBJECTIVE: To explore the biological characteristics of CD90+ tumor stem cels in ovarian cancer cels.
METHODS:The CD133 and CD90 positive rate of SKOV3 and primary ovarian cancer cels were detected by flow cytometry. The CD90+ and CD90- relative expression in stem cels and epithelium was detected by RT-PCR. Transwel invasion assay was employed to observe the cel invasion ability, clone formation test was done to observe cel proliferation and differentiation capacity, suspension bal test was adopted to observe pluripotent stem cels. The tumor formation time and tumor formation rate were observed by limited tumor dilution in immunodeficient mice.
RESULTS AND CONCLUSION:The positive rates of CD133 and CD90 in SKOV3 were significantly lower than those in primary ovarian cancer cels. The expression of CD133 and OCT4 in CD90+cels of SKOVS was significantly higher than that in CD90-cels of SKOVS. The expression of CD44, CD133, acetaldehyde dehydrogenase-1 and OCT4 in CD90+stem cels of primary ovarian cancer cels was significantly higher than that in CD90-stem cels of primary ovarian cancer cels. There were significant differences in the epithelial-mesenchymal related gene expressions between CD90-and CD90+stem cels of SKOV3 and primary ovarian cancer cels. With the increase of inoculated cels, the tumor formation rate of CD90-and CD90+ cels was increased continuously, but the tumor formation time was decreased. The tumor rate of CD90-cels was lower than that of CD90+cels. The number of transmembrane cels, cel clones and suspended cel bals was significantly higher in the CD90+ stem cels than the CD90-stem cels. These findings indicate that in ovarian cancer cels, CD90+stem cels can highly express stem cel-related genes and epithelial-mesenchymal related genes, which have a higher invasion, proliferation and differentiation ability, as wel as tumorigenic and pluripotent ability.

Objective To investigate the correlation between plasma N-terminal pro-brain natriuretic peptide (NT-proBNP) and haemodynamics evaluated by right-heart catheterization in patients with chronic thromboembolic pulmonary hypertension (CTEPH).Methods Fifty-three patients with CTEPH were retrospectively reviewed.All patients underwent right-heart catheterization to detect haemodynamics.The level of plasma NT-proBNP was measured by electrochemical luminescence.The indexes were analyzed.Results Pulmonary artery systolic pressure was (84.9 ± 21.6) mmHg (1 mmHg =0.133 kPa).Pulmonary artery diastolic pressure was (31.1 ± 9.7) mmHg.Pulmonary artery pressure was (49.3 ± 13.4) mmHg.Pulmonary vascular resistance was (1 047.4 ± 86.1) dyn ·s/cm5.Pulmonary capillary wedge pressure was (8.16 ± 3.02) mmHg.Right cardiac output was (3.36 ± 1.42) ml.Right cardiac work was (1.97 ± 0.95) kg·m.The level of plasma NT-proBNP in CTEPH was (2 301.5 ± 1 787.3) ng/L.The level of plasma NT-proBNP in CTEPH had positive correlation with pulmonary vascular resistance (r =0.429,P =0.02),and had negative correlation with right cardiac output (r =-0.583,P＜ 0.01) and right cardiac work (r =-0.521,P ＜ 0.01).The level of plasma NT-proBNP in CTEPH had no correlation with pulmonary artery systolic pressure,pulmonary artery diastolic pressure,pulmonary artery pressure,pulmonary capillary wedge pressure (P ＞ 0.05).Conclusion The level of plasma NT-proBNP can be used as a better predictor for evaluating pulmonary vascular resistance and right cardiac function in CTEPH.

Objective To investigate the role of autonomic nerve system(ANS)in the pathogenesis of acute mountain sickness(AMS).Methods A hypobaric chamber was used to simulate 4500m altitude for 1 day.The function of ANS of 43 healthy young males was measured individually before the experiment,and then at a “height” of 4500m for 5min.GJB1098-91 clinical questionnaires were conducted to evaluate the AMS symptomatic scores.HRV was measured respectively before the test and 2h,10h,20h at 4500m;cold pressure test(CPT)and plasma concentration of NA were determined before the test and at 4500m,AMS scores were evaluated at the end of exposure.25 subjects with AMS scores of 10 or less were identified as mild AMS group(group A),and another 18 subjects with scores of over 10 were gathered as moderately severe AMS group(group B).Results Compared with group A,the systolic pressure(SP)during CPT increased markedly in group B(P

Brugada Syndrome ; etiology ; physiopathology ; Coronary Artery Disease ; physiopathology ; Coronary Vasospasm ; physiopathology ; Electrocardiography ; Humans ; Male ; Middle Aged