With studying different kinds of papers domestic and abroad about masking of tinnitus and measurement of tinnitus sound,we made sure that the masking device can cure tinnitus to certain extent.After considering various factors affecting the result of the masking.We selected the narrow-band noise to be the main kind of masking sound of tinnitus and determined the practical values of quality factor (Q) and ratable range of center frequency(f_0) of the narrow—band fiter.The last paragraph of paper gives the brief description to the prototype masking device.

AIM: To develop a GC/MS method for analyzing volatile compounds in Liushen Pill and Japanese(Jiuxin) Pill. METHODS: The gas chromatograph was fitted a fused-silica capillary column with a VF-5 ms stationary phase and dimensions:30 m?0.25 mm i.d.,0.25 ?m film thickness.The carrier gas was helium at a flow-rate of 1 mL/min.Injector temperature was at 280 ℃ and operated in the splitless mode.The temperature of the GC-MS transfer line was at 250 ℃.Full scan electron impact ionization data were acquired under the following condition:solvent delay 6 min.70 eV electron impact energy,scan time 0.87 sencond/scan,emission current 80 mA,manifold and trap temperatures 45 ℃ and 170 ℃. RESULTS: The quantitative method was accepted with good specificity,wide linear range and low LOD.At the same time,the content of isoborneol was determined by a semi-quantitative approach. CONCLUSION: The research is propitious to offer an effective and reliable pattern for analyzing volatile compounds in complex composite formulae.

Attention deficit hyperactivity disorder(ADHD) usually occurs before the age of 7.But it still remains controversy in the diagnosis,intervention and treatment of ADHD among preschool children with symptoms,who are younger than age 7.This paper systematically describes the diagnosis and intervention on the basis of the recent researches of ADHD home and abroad.

Objective To evaluate the value of renal adipose capsule injection of methyprednisolone by ultrasound guided in treatment of nephritic syndrome.Methods 31 patients with nephritic syndrome were treated with the renal adipose capsule injection of methyprednisolone by ultrasound guided.40mg methylprednisolone was injected into renal adipose capsule of the patients twice a week and each one of them was totally given ten times of the treatment.The urinary protein,plasma albumin,serum creatinine and blood pressure before and after the treatment were measured.Results 29 cases were succeed in this group,and the percentage was 94%,clinical observations of proteinuria were negative.2 cases were repeated after stop interventional treatment.and the percentage was 6%.Conclusion By ultrasound guided interventional treatment and capsula adipose treated for nephrotie syndrome was simple operation,and had precise positioning and significant effect,and it provided a new treatment for clinic.

Radix et Rhizoma Clematidis as a key traditional Chinese medicine with function of wind-dampness-dispelling and stasis-resolving has been widely used in clinical of Chinese medicine.For the further study and development of Radix et Rhizoma Clematidis, we reviewed the identification, processing, chemical constitutes and biological activities in this article.

Aim To explore the protective effect of SMT on acute cerebral infarction.Methods Focal cerebral infarction was induced by photochemistry.The expression of iNOS was detected in microglia by double fluorescent immunohistochemistry and laser scanning confocal microscopy. NO content at post-ischemic time points was determined by electron spin resonance(ESR) technique and standard addition method.TTC stain was used to determine the infarct volume.Results At ischemic early period,compared with control group,NO content was not remarkably different after 2 h,6 h of ischemia in the treatment groups,infarct volumes also were not obviously changed.At ischemia mid and late period 12 h,24 h,48 h,the expression of iNOS and NO content was suppressed by SMT treatment in microglia.In TTC stain results,infarct volumes were increased obviously after 12 h,and achieved the peak after 24 h,48 h in control groups,infarct volumes in treatment groups were significantly decreased after 12 h,24 h,48 h.Conclusion SMT may play a protective role in acute cerebral infarction by reducing the expression of iNOS and NO content in microglia.

AIM:To explore the regulating mechanism of microglia transplantation in treating cerebral infarction.METHODS:Focal cerebral infarction model was established by photochemistry. The histological and immunohistochemical methods were used to observe the effects of microglia transplantation on cerebral infarction. The expressions of nerve growth factor(NGF) and interleukin-10(IL-10) were detected by double fluorescent immunohistochemistry and Western blotting.RESULTS:Microglia marked with green fluorescence was observed in ischemic penumbra,indicating that microglia can be transplanted across the blood-brain barrier. Infarct volumes and death number of cells were significantly reduced compared to non-transplantation animals. The expressions of NGF and IL-10 were markedly increased in microglia in transplantation group.CONCLUSION:Microglia can be transplanted across the blood-brain barrier. The cells have protective effects in ischemic penumbra by secreting NGF and IL-10,which might serve as therapeutical method for treating cerebral infarction.

OBJECTIVE To explore the antagonistic effect of calpain inhibitor Ⅲ MDL 28170 on acute methylmercury (MeHg) neurotoxicity. METHODS The rats were randomly divided into MDL 28170 (50 mg·kg~(-1),ip) control group, MeHg (10 mg·kg~(-1),ig), and MeHg (10 mg·kg~(-1),ig)+MDL 28170 (50 mg·kg~(-1), ip) group. The escape latencies were observed by Morris water maze test. The neurons of positive μ-calpain were determined by immunohistochemical method, the expression of μ-calpain was analyzed by Western blotting, apoptotic neurons were observed by TUNEL method, and microtubule-associated protein 2 (MAP2) expression was detected in neuron by immunohistochemical method. RESULTS MeHg was ig given for 3-7 d, the initial signs of behavioral changes developed in MeHg group, the escape latencies were lengthened in Morris water maze test (P<0.01). The expression and activity of μ-calpain elevated obviously in parietal cerebral neurons(P<0.01), neuronal apoptosis was markedly increased(P<0.01), and the decomposition of MAP2 was reduced in cortical neurons obviously. And the above changes were suppressed obviously by treatment of MDL 28170. CONCLUSION μ-Calpain may correlate with neuronal apoptosis induced by MeHg, MDL 28170 markedly reduces neuronal apoptosis, which might act as therapeutical method for MeHg poison.

Objective To observe the clinical efficacy in treatment on diabetic peripheral neuropathy (DPN) by Chinese medicine lavipeditum of blood-activating and Qufengchushi with mecobalamin tablets. Methods 65 diabetic patients were randomly divided into 2 groups. The two groups were both undertaking the diabetes diet control, exercise, diabetes education and maintaining previous hypoglycemic program. The patients with hypertension could take nifedipine GITS. Patients in control group only received mecobalamin tablet. Patients in treatment group received the same medication and also lavipeditum with Blood-activating and Qufengchushi traditional Chinese medicine for 12 weeks. Clinical symptom score, Toronto clinical score, motor nerve conduction velocity (MNCV) and sensory conduction velocity (SNCV) of median nerve and peroneal nerve were performed before and after the study. Results After treatment, the mean values for clinical symptom score, Toronto clinical score, MNCV and SNCV of median nerve and peroneal nerve of the tweo groups were all significantly decreased (P<0.05, P<0.01). The values for TCM clinical syndromes in treatment group was 81.81% (27/33), and that in the control group was 65.62% (21/32), with significant difference (P<0.05). The values for western medcine clinical syndromes in treatment group was 84.84% (28/33), and that in the control group was 62.50%(20/32), with significant difference (P <0.05). Conclusion Chinese medicine lavipeditum of Blood-activating and Qufengchushi with mecobalamin tablets is safet and effective in treating DPN.

Apoptosis ; drug effects ; Asbestos ; toxicity ; Humans ; Mineral Fibers ; toxicity