Objective:To evaluate the influence of rhCD40L on mononuclear macrophage,U937 cells,secreting matrix metalloproteinases(MMPs) and its possible mechanism.Methods: U937 cells were treated with different concentrations of rhCD40L and NS-398(specific antagonist of COX-2) for 24 h and the supernatants were harvested.Cells treated with 0.4 ?g/ml rhCD40L were further treated with 10~(-4) mol/L NS-398 or aspirin separately for 24 h and the supernatants were harvested.Zymography was used to determine the activities of MMPs in the above supernatants.Results: rhCD40L increase the activity of MMP-2 and MMP-9 in a dose-dependent manner(P

Ethics, Medical ; Humans ; Medicine

It is very difficult to measure the human eye properties directly, such as the accommodation mechanism, intraocular pressure distribution, the dynamics of aqueous humor flow and the bio-heat transfer in human eyes. Modeling and simulation may, therefore, play an increasingly important role in the ophthalmologic investigation. The major computer modeling methods, including geometric modeling, physical modeling and mathematical modeling, are introduced in this paper. Modeling and simulation anatomy properties and physiological properties of eye tissues, such as the cornea, aqueous humor and crystalline lens, vitreous, optic nerve head, sclera, are analyzed in the order from global to local, from front to back, from outside to inside. Finally, the problems of computer modeling in ophthalmologic investigation are discussed, and the development trends of the future are pointed out.
Aqueous Humor ; physiology ; Computer Simulation ; Cornea ; physiology ; Eye ; anatomy & histology ; Humans ; Lens, Crystalline ; physiology ; Ophthalmology ; trends ; Sclera ; physiology

Gene expression profile technology is characterized by high automation, high accuracy and large information volume, which provides a new way for the development of individualized therapies and novel therapeutic targets. Some researches have identified sets of discriminating genes by using gene expression profile technology that can be used for the predietion of response to radiotherapy in human cancers. Some studies also have confirmed the therapeutic tagerts of radiation sensitizer.

Microglia, the main innate immune cells in the central nervous system, takes part in lots of physiological and pathological processes in the brain.It not only maintains brain homeostasis but also participates in the process of brain injury and repair under pathological conditions.In developmental brain, microglial synaptic pruning may eliminate weaker synapses and retain stronger synapses.Synaptic pruning also plays a vital role in mediating the formation of neural circuit under physiological condition, contributes to cell and myelin debris clearance, promotes maturation of oligodendrocytes, which surround the bare axon to form myelin sheath, and helps the regeneration of neurons and synapses.Recently, increasing number of studies on microglial synaptic pruning has advanced our understanding of the underlying mechanism for synaptic pruning and its relevant physiological functions.Here, we reviewed microglial synaptic pruning function and its potential regulatory mechanisms in brain under physiological and pathological conditions.

Non-small cell lung cancer(NSCLC) is one of the most life-threatening human malignancies due to its high morbidity and mortality rates.Molecular targeted therapy is promising in treating some patients with NSCLC.However,it is still challenging to select proper candidates.The EML4-ALK fusion oncogene represents a novel molecular target which appears mainly in lung adenocarcinoma,and appears to be a potential biomarker associated with resistance to EGFR tyrosine kinase inhibitors.This review was intended to outline current status of preclinical and clinical research of this molecule.

Objective: To evaluate the influence of rhCD40L on mononuclear macrophage, U937 cells, secreting matrix metalloproteinases (MMPs) and its possible mechanism. Methods: U937 cells were treated with different concentrations of rhCD40L and NS-398 (specific antagonist of COX-2) for 24 h and the supernatants were harvested. Cells treated with 0.4 μg/ml rhCD40L were further treated with 10-4 mol/L NS-398 or aspirin separately for 24 h and the supernatants were harvested. Zymography was used to determine the activities of MMPs in the above supernatants. Results: rhCD40L increase the activity of MMP-2 and MMP-9 in a dose-dependent manner (P<0.01); the increased peaked when rhCD40L was at 0.4 μg/ml. NS-398 inhibited the activity of MMP-2 and MMP-9 in a dose-dependent manner (P<0.05). NS-398 and aspirin both significantly inhibited the activity of MMP-2 and MMP-9 induced by rhCD40L (P<0.05). Conclusion: rhCD40L can induce U937 cells to secrete MMPs in a dose dependent manner, which might be related to COX, more possibly through COX-2 than COX-1 pathway.

Small cell lung cancer (SCLC) is an aggressive malignancy with fairly poor prognosis. Innovative treatment based on improved understanding of the genetic alterations of SCLC is awaited. Recently, a number of potential targets or important oncogenic pathways have been identified by the next generation sequencing or other systematic genomic analysis in SCLC. In this review, we summarised the new ifndings of genetic characteristics in SCLC.

Objectives: To investigate the occurrence and risk factors of cerebral microbleeds on the cognitive function in patients with ischemic cerebrovascular disease and to investigate the effect of cerebral microbleeds on the cognitive function in patients with ischemic cerebrovascular disease. Methods: A total of 160 patients with ischemic cerebrovascular disease were divided into cerebral microbleed group (n =30) and control group (n = 130) according to MR T2 *-weighted gradient echo sequences. The demographic data of the patients, conventional risk factors, sites of microbleed, types of cerebral infarction, severity of white matter changes, and numbers of lacunar infarction were recorded in detail. The Montreal Cognitive Assessment was conducted in both groups. All the data were processed using SPSS 16.0 software. Results: Circled digit oneThe mean age of the microbleed group was older than that in the non-microbleed group (75 ±9 vs. 66 ±6, P = 0.000) ; the degree of white matter changes was higher (6.7 ±2.4 vs. 3.5 ±1. 8, P =0.003) ; and the numbers of lacunar infarction were larger (5.0 ±2. 3 vs. 2. 1 ±1.3, P= 0.000). Circled digit twoThe logistic regression analysis showed that the high blood cholesterol, the degree of white matter change ( WMC ) , and the numbers of lacunar infarction were the independent risk factors for CMB (OR =3. 880, 95% CI:0. 086 -0. 994; OR = 11.735, 95% CI; 1.340 -2.930; OR = 10. 160, 95% CI: 1.241 -2.475). Circled digit threeThere was no significant difference in the cognitive function scores between the two groups ( P > 0. 05 ). Circled digit fourThere was significant difference in the executive ability between the two groups (P =0.010). Conclusions: Circled digit oneCerebral microbleeds are closely associated with leukoaraiosis and lacunar infarction; Circled digit twothe patients with ischemic cerebrovascular disease accompanied by microbleeds has obvious executive dysfunction ; Circled digit threecontrolling the risk factors for microbleeds has a certain role for the control of the occurrence and development of cognitive impairment in patients with ischemic cerebrovascular disease.