Objective To investigate the expression of tight junction protein 1(Claudin‐1) and vascular endothelial growth factor C(VEGF‐C) in colorectal carcinoma tissues and the relationship between them .Methods Totally 50 cases of colorectal carci‐noma ,paracancerous tissues and 50 cases of normal colorectal tissues from Department of Pathology were detected by immunohisto‐chemistry SP method ,according to the patient′s age ,gender ,lymph node metastasis ,depth of invasion ,clinical stage for comprehen‐sive comparison ,and analyze the Claudin 1 and VEGF‐C protein expression in colorectal cancer tissues .Results The positive rates of Claudin‐1 ,VEGF‐C in colorectal cancer tissues was significantly higher than that in paracancerous tissue and normal colorectal tissues (χ2 =32 .270 ,P=0 .000;χ2 =41 .209 ,P=0 .000) ,the positive rate of Claudin‐1 ,VEGF‐C in paracancerous cancer tissues was significantly higher than that in normal colorectal tissues (χ2 =7 .294 ,P=0 .007 ;χ2 = 5 .741 ,P=0 .017) .The expression of colorectal carcinoma Claudin‐1 had close relationship with differentiation ,invasion degree ,TNM stage ,lymph node metastasis(P<0 .05) ,the expression of VEGF‐C had colse relatons with infiltration degress ,TNM stage ,lymph node metastasis ,tumor size(P<0 .05) .The expression of colorectal cancer Claudin‐1 ,VEGF‐C had significantly positive correlation (χ2 =10 .953 ,P= 0 .001 ,r=0 .468) .Conclusion The expression of colorectal cancer Claudin‐1 ,VEGF‐C was significantly higher than that in paracancerous tis‐sue and normal colorectal tissue ,the expression of Claudin‐1 ,VEGF‐C had a certain relationship with clinical staging .

Lung cancer is one of the malignant tumors with highest incidence recently in our country. Tumor hypoxia phenomenon was ifrst discovered in lung cancer. It plays important roles in tumor cell drug-resistance, apoptosis, inva-sion and metastasis, angiogenesis. Chemotherapy-resistant is one of the core reasons of treatment failure and disease progress, and many works on the study of chemotherapy-resistant have been done. hTis article reviewed the research progress of those mechanisms by which lung cancer hypoxia microenvironment could induce chemotherapy-resistance.

Elaphurus davidianus( Milu),a rare animal unique to China,has been used as medicine for more than a thousand years,but the extinction of Milu in modern times resulted in the unavailability of related medical products. Today,the reintroduction of Milu population makes it possible to restore its medicinal usage. The resource reserves of Cervi Cornu,the natural shedding product from Milu,are increasing with the expansion of the population,allowing it to be fully utilized in the medical field. Mijiao Pills,first recorded in Important Prescriptions Worth a Thousand Gold for Emergency( Bei Ji Qian Jin Yao Fang) by Sun Simiao in the Tang Dynasty,is the first Chinese medicinal prescription with Cervi Cornu as the sovereign medicinal,which is effective in tonifying. Its composition,preparation,efficacy and indications,and administration are described in detail in the Important Prescriptions Worth a Thousand Gold for Emergency,which however,have changed significantly over the thousands of years,seriously affecting the clinical application of this classical prescription and related product development. Therefore,the key information of this prescription should be systematically collated and summarized. According to the principles of textual research on key information in ancient classical prescriptions promulgated by relevant authorities,this paper reviewed ancient Chinese medical books of the past dynasties,modern literature,as well as the Pharmacopoeia of the People's Republic of China( 2020 Version) to figure out such key information as the source,historical evolution,original plants and animals and their processing,dosage,preparation,and usage of Mijiao Pills. This paper aimed to provide a basis for the clinical application of Mijiao Pills and subsequent product development,thus facilitating the development and utilization of this precious medicinal animal resource.
Books ; China ; Cornus ; Humans ; Medicine, Chinese Traditional ; Prescriptions

In order to reveal the regional characteristics of nucleosides and amino acids in Elaphuri Davidiani Cornu,39 samples of Elaphuri Davidiani Cornu collected from 4 different regions were analyzed by UPLC-QTRAP ~®/MS2 method followed by orthogonal partial least-squares discrimination analysis( OPLS-DA) and cluster analysis( CA). The results showed all the samples contained abundant nucleosides and amino acids,with the total content of 45. 09 μg·g~(-1) and 634. 80 μg·g-1,respectively. The samples presented significant regional differences in the contents of individual components,and the main differential components included Ura,Hpro,Thr,Glu,G5 P,2'-dG,Adeno,Met,Ade,Gln,Orni,Phe,2'-dA,Hit,Lys,and Ile. Among them,Ura,Met,Glu,and Ile had the highest content in the samples from Dafeng in Jiangsu,Qinhu in Jiangsu,Beijing,and Shishou in Hubei,respectively. OPLS-DA and CA demonstrated that all the samples of Elaphuri Davidiani Cornu could be divided into three categories,reflecting the regional characteristics. The results indicated that the accumulation of nucleosides and amino acids in Elaphuri Davidiani Cornu was closely related to its habitat,providing a useful reference for the research on the quality formation,quality evaluation and control,as well as the comprehensive utilization of Elaphuri Davidiani Cornu. The findings suggested that the content factors of Ura,Met,Glu,and Ile could be included into the quality standard system of Elaphuri Davidiani Cornu as the characteristics of medicinal materials from different regions.
Amino Acids ; Beijing ; Cornus ; Nucleosides