1.Applied study on magnetic nanometer beads in preparation of genechip samples.
Hui, CHEN ; Huafang, GAO ; Xin, XIE ; Xuemei, MA ; Yuzhen, YANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2004;24(1):25-7
A protocol for enrichment and adsorption of karyocyte from whole blood by using magnetic nanometer beads as solid-phase absorbents was presented. The PCR amplification could be accomplished by using the nanobeads with karyocyte as template directly and the PCR products were applied on an oligonucleotide array to do gene typing. The HLA-A PCR amplification system and a small HLA-A oligonucleotide microarray were applied as the platform and an experiment protocol of separating karyocyte from whole blood using the magnetic nanometer beads (Fe2O3) were set up. The experimental conditions were also discussed. It showed that pH level of PBS eluent, Taq enzyme quantity and fragment length of products could influent the amplification results, and the magnetic nano-beads could succeed in sample preparation in microarray to provide a promising way in automatic detection and lab-on-a-chip.
Adsorption
;
Cell Separation/instrumentation
;
Cell Separation/methods
;
Genotype
;
Leukocytes/*cytology
;
*Magnetics
;
Microchemistry/instrumentation
;
Microchemistry/*methods
;
Nanotechnology
;
Oligonucleotide Array Sequence Analysis/*methods
;
Polymerase Chain Reaction
;
Templates, Genetic
2.Advances in piezoelectric quartz crystal biosensor and its applications.
Journal of Biomedical Engineering 2004;21(2):316-320
Piezoelectric quartz crystal biosensor is a new sensor developed with the comprehensive utilization of the high sensitivity to mass and the surface characteristics of quartz crystal such as density, viscosity, dielectric constant, conductance, and the high specificity of biologic identification molecules. It has the features of high sensitivity, high specificity, simple operation, high analysis speed, low cost, small size, on-line detection, etc. It can be of wide application in the areas of medical laboratory diagnosis, environment monitor, foods sanitary control and industrial production. In this paper, the fundamental principle, structure and its applications are reviewed.
Biosensing Techniques
;
instrumentation
;
methods
;
Crystallization
;
DNA, Viral
;
analysis
;
Elasticity
;
Electrodes
;
Equipment Design
;
methods
;
HIV
;
isolation & purification
;
Microchemistry
;
Quartz
3.Applied study on magnetic nanometer beads in preparation of genechip samples.
Hui CHEN ; Huafang GAO ; Xin XIE ; Xuemei MA ; Yuzhen YANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2004;24(1):25-27
A protocol for enrichment and adsorption of karyocyte from whole blood by using magnetic nanometer beads as solid-phase absorbents was presented. The PCR amplification could be accomplished by using the nanobeads with karyocyte as template directly and the PCR products were applied on an oligonucleotide array to do gene typing. The HLA-A PCR amplification system and a small HLA-A oligonucleotide microarray were applied as the platform and an experiment protocol of separating karyocyte from whole blood using the magnetic nanometer beads (Fe2O3) were set up. The experimental conditions were also discussed. It showed that pH level of PBS eluent, Taq enzyme quantity and fragment length of products could influent the amplification results, and the magnetic nano-beads could succeed in sample preparation in microarray to provide a promising way in automatic detection and lab-on-a-chip.
Adsorption
;
Cell Separation
;
instrumentation
;
methods
;
Genotype
;
Leukocytes
;
cytology
;
Magnetics
;
Microchemistry
;
instrumentation
;
methods
;
Nanotechnology
;
Oligonucleotide Array Sequence Analysis
;
methods
;
Polymerase Chain Reaction
;
Templates, Genetic