Objective:To study the effect of various extract of Sini Decoction on hypothalamus-pituitary-sexual glands axle of hypothyroidism rats with syndrome of yang deficiency, and discuss the mechanism of Sini Decoction in reinforcing yang. Methods: The model of deficiency of both the spleen-yang and the kidney-yang were induced by PTU solution. After the model were confirmed, the rats in Sini Decoction group were administered with Sini Decoction . At the end of the experiment, all the rats were sacrificed and the blood were sampled to check the level of Sex hormones , the reproductive organs were weighed, the testicle were sliced to check their pathological change. Results:Various extract of Sini Decoction can reduce the increase of weight of orchis and prostate of model rats, reduce their pathologic damage, and increase the level of testosterone. The alcohol extract of Sini Decoction has obvious therapetic effects. Conclusion: Sini Decoction can improve the sexual hormone level and weight index of reproductive organs of hypothyroidism rats with syndrome of yang defi ciency.

Objective:To establish the quality standard for Sonehus braehyotus DC. Methods:The pharmacognosy of Sonehus braehyotus DC. were studied,and the qualitative identification by TLC and the quantitative analysis by HPLC were established. Re-sults:The microscopic characteristics of Sonehus braehyotus DC. were obvious. The TLC spots were clear with high resolution,and the quantitative research of chlorogenic acid in 10 batches of Sonehus braehyotus DC. was performed by HPLC. Conclusion:The quality control standard for Sonehus braehyotus DC. is established through the experimental studies.

OBJECTIVETo determine the change pattern of alkaline and acid components of Dahuang and Fuzi in different combinations.

METHODThe contents of anthraquinones and aconite alkaloids were determined by HPLC in samples of Dahuang extracts, Fuzi extracts, Dahuang and Fuzi pair medicines extracts, and extracts of whole recipe.

RESULTAs for Dahuang, the contents of anthraquinones were decreased with changes of combination: higher contents were in decoctions of Dahuang alone, lower contents were in decoctions of pair medicines, and the lowest contents were in decoctions of whole recipe. As for Fuzi, monoester aconite alkaloids could be detected in water extracts, and both biester and monoester aconite alkaloids could be detected in ethanol extracts. The contents of aconite alkaloids were decreased with changes of combinations: from Fuzi alone to pair medicines, and to whole recipe. The change pattern was more apparent when extracted in water decoction.

CONCLUSIONThe contents of alkaline and acid components are changed with different extract methods and different combinations.

Acids ; analysis ; Aconitum ; chemistry ; Alkalies ; analysis ; Alkaloids ; analysis ; Anthraquinones ; analysis ; Drugs, Chinese Herbal ; chemistry ; Rheum ; chemistry

OBJECTIVETo investigate the change regularity of ephedrine and glyrihhzine acid in Ephedra sinila and Glycyrrhiza uralencis pair medicines and in Maxing Shigan decoction.

METHODThe contents of ephedrine and glycyrrhizic acid were determined by HPLC in samples of E. sinica extracts, G. uralencis extracts, pair medicines extracts of Maxing Shigao decoction sinica and G. uralencis, and extracts of E. sinica.

RESULTThere were no significant difference in ephedrine contents amoung different samples; the contents of glycyrrhizic acid were lower in decoctions of Maxing Shigan decoction than in G. uralencis decoction and pair medicines extracts.

CONCLUSIONMacromolecular complex was NOT formed by ephedrine and Glycyrrhizic acid in decoctions containing pair medicines of E. sinica and G. uralencis.

Chemistry, Pharmaceutical ; Drugs, Chinese Herbal ; chemistry ; Ephedra sinica ; chemistry ; Ephedrine ; chemistry ; Glycyrrhiza ; chemistry ; Glycyrrhizic Acid ; chemistry

OBJECTIVETo determine the change pattern of alkaline and acid components of Dahuang and Huangbai amoung different combinations in Dahuang Xiaoshi decotion.

METHODThe contents of anthraquinones and berberine were determined by HPLC in samples of Dahuang extracts, Huangbai extracts, Dahuang and Huangbai pair medicine extracts, and extracts of whole recipe.

RESULTThe contents of anthraquinones and berberine were decreased with the changes of combinations: from component medicinal material alone to pair medicines, and to whole recipe. the change pattern was more apparent in water decoctions than in ethanol extracts.

CONCLUSIONThe contents of alkaline and acid components are changed with different extract methods and different combinations.

Acids ; analysis ; Alkalies ; analysis ; Drugs, Chinese Herbal ; chemistry ; Phellodendron ; chemistry ; Rheum ; chemistry

OBJECTIVETo determine the change pattern of alkaline and acid components of Fuzi and gancao amoung different combinations in Sini Decotion.

METHODThe contents of aconite alkaloids and glycyrrhizic acid were determined by HPLC in samples of Fuzi extracts, gancao extracts, Fuzi and gancao pair medicines extracts, and extracts of whole recipe.

RESULTAs for Fuzi, monoester aconite alkaloids could be detected in water extracts, and both biester and monoester aconite alkaloids could be detected in ethanol extracts. The contents of aconite alkaloids were decreased with changes of combinations: from Fuzi alone to pair medicines, and to whole recipe. The change pattern was more apparent when extracted in water decoction. As for Gancao, the glycyrrhizic acid was lowered from single medicine to pair medicines, and to whole recipe.

CONCLUSIONThe contents of alkaline and acid components were changed with different extract methods and different combinations of Fuzi and Gancao.

Acids ; analysis ; Aconitum ; chemistry ; Alkalies ; analysis ; Alkaloids ; analysis ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; chemistry ; Glycyrrhizic Acid ; analysis

OBJECTIVETo study the chemical structures of macromolecular complexes formed by aconitine and glycyrrhizic acid, aconitine and rhein, berberine and rhein, ephedrine and glycyrrhizic acid in decoctions.

METHODThe binding energy of reactants and products was determined by X-ray spectrography, the ground level charge distribution of products was caculated by density function theory of quantum chemistry.

RESULTThe binding energy of aconitine and glycyrrhizic acid, aconitine and rhein, berberine and rhein were changed after reaction. The characteristic atoms in the molecular structures of acid components have higher positive electric charge, while the ones in alkaline components have higher negative charge.

CONCLUSIONAconitine and glycyrrhizic acid, aconitine and rhein, berberine and rhein can form macromolecular complexes, ephedrine and glycyrrhizic acid can not.

Acids ; chemistry ; Alkalies ; chemistry ; Drugs, Chinese Herbal ; chemistry

Compatibility chemistry of acid-alkaline pair medicines in formulas of traditional Chinese medicine (TCM) is an important research field which should merit to pay attention. The ideas and methods in prescription compatibility research on formulas containing alkaline-acid pair medicines were summarized from the aspect of chemical groups of alkaline and acid ingredients; the research results were introduced and analyzed; the research meaning was elaborated; and the expectation of the field was viewed.
Animals ; Chemistry, Pharmaceutical ; methods ; Drug Incompatibility ; Drugs, Chinese Herbal ; chemistry ; Humans ; Medicine, Chinese Traditional ; Research

OBJECTIVE To establish the high -performance liquid chromatography （HPLC）fingerprint of Compound huiqin granules and analyze it with chemical pattern recognition ，and determine the contents of 8 active components in the granules . METHODS Using puerarin as reference peak ，the fingerprints of 10 batches of Compound huiqin granules were drawn by HPLC method. Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine （2012 edition）was used to evaluate the similarity and identify common peaks ；SPSS 22.0 and SIMCA 14.0 software were used for cluster analysis and principal component analysis . HPLC method was used to determine the contents of 8 active components ，such as chlorogenic acid ， puerarin，3′-methoxypuerarin，daidzin，luteolin-7-O-β-D-glucuronide，isochlorogenic acid A ，and apigenin -7-O-β-D-glucuronide and isochlorogenic acid C . RESULTS There were 26 common peaks in the fingerprints of 10 batches of Compound huiqin granules，the similarity was greater than 0.900，and 11 peaks were identified ，which were gallic acid ，neochlorogenic acid ， chlorogenic acid ，puerarin，3′-methoxypuerarin，daidzin，luteolin-7-O-β-D glucuronide ，isochlorogenic acid A ，apigenin-7-O-β-D- glucuronide，isochlorogenic acid C and daidzein . The results of cluster analysis and principle component analysis showed that 10 batches of Compound huiqin granules could be clustered into 4 categories，in which S 2，S3 and S 6 belonged to one category ，S4， S8，S10 belonged to one category ，S7 belonged to one category ，and S 1，S5，S9 belonged to one category . Average contents of 8 active components were 1.30，17.42，3.51，3.57，0.75，0.41，0.31，0.32 mg/g，respectively. CONCLUSIONS In this study ，the fingerprints and multi -component content determination method of Compound huiqin granules are successfully established ，which can provide a basis for the quality control of the granules .