Objective To investigate the effect of subretinal fluid (SRF) with different grades of proliferative vitreoretinopathy (PVR) on bcl-2 oncoprotein expression in retinal pigment epithelium (RPE) cells and fibroblast (FB). Methods Using immunohistological staining technique and Western-blotting method to detect the expression of bcl-2 protein in RPE cells and FB under the stimulation of SRF. Results The expression levels of bcl-2 increased in both types of cells to a certain extent compared with those of the control group 4 hours after the cells subjected to SRF; 36 hours later, the expression levels of bcl-2 of experimental groups decreased more quickly than those of the control group,and the control group showed relatively higher bcl-2 protein levels at the end of observation. Conclusions SRF may stimulate the expression of bcl-2 in RPE cells and FB under culture at early stage, but accelarate the declining of bcl-2 protein levels a certain time after subjected to SRF.

Objective To investigate the effect of subretinalfluid (SRF) with different grades of proliferative vitreoretinopathy (PVR) onｂcl-2 oncoprotein expression in retinal pigment epithelium (RPE) cells and fib roblast (FB)．Methods Using immunohistologicalstain ingtechniｑue and Westernblotting method to detect the expression of ｂcl-2 protein in RPE cells and FB under the stimulation of SRF．Res u ltsThe expression levels of ｂcl-2 increased in both types oｆcells to a certain extent compared with those of the control group 4 hours afterthe cells subjected to SRF； 36 hours later, the expression levels of ｂcl-2 o f experimental groups decreased more quickly thanthose of the controlgroup， and the controlgroup showed relatively higher ｂcl-2 protein levels at the end of observation． Conclusions SRF may stimulate the e xpression of ｂcl-2 in RPE cells and FB under culture at early stage, but accel arate the declining of ｂcl-2 protein levels a certain time after subjected toSRF．

ObjectiveTo detect the effect of subretinal fluid （SRF） from different grades of proliferative vitreoretinopathy（PVR） on cultured retinal pigmental epithelial cells（RPE） and fibloblasts（FB）．MethodsThe effect of SRF from different grades of PVR on RPE and FB was determined using Brdu labeling technique and MTT assaying method，respectively．Results All samples showed a stimulating effect on the two kinds of cellular elements in a certain degree．At 1∶10 concentration，the cellular proliferation stimulating activity of SRF with PVR＜C1，PVR C1 and PVR＞C1 on RPE and FB was 128.5%，139.8%，156.8%and 126.3%，143.1%，172.3%of the control groups, respectively after 24 h stimulation.ConclusionsSRF’s proliferation-stimulating activity is correlated with the grades of PVR．

Objective To analyze the training effect for junior nurses from internal medical departments of our hospital.Methods Data gathering tool,based on the Kirkpatrick Four Levels,was formulated by means of theoretical research and expert consultation.Before training,investigation of needs was conducted and then training contents were organized.Objective structured clinical examination (OSCE) was implemented before and after the training to obtain information about nurses' comprehensive abilities and to compare with their self-assessed data.Twelve months after the training,the training effect was kept track on from those subjects' head nurses.Results After the training,80 subjects' comprehensive abilities obtained from OSCE scored higher than before the training,(71.51±0.49) vs.(61.92±0.55),t=4.961,P ＜ 0.01.Their selfassessed comprehensive abilities also scored higher than that before,(83.49±0.75) vs.(72.73 ±0.43),t=7.020,P ＜ 0.01,the differences were both statistically significant.By questionnaire training,26 head nurses (26/32,88.46％) in general medical department showed positive assessment over training effect.Nurse's behaviors had improved significantly.In 80 training nurses,60 (75.00％) had become the examples of their colleagues.In 80 patients and their family members,73 cases showed their satisfaction and the satisfaction degree was higher than 90％.Conclusions The Kirkpatrick Four Levels can be applied to assess the training effect for junior nurses and the training mode can effectively improve comprehensive abilities of junior nurses.

Objective To explore job satisfaction degrees and the influencing factors among community nurses. Method General information questionnaire and the revised job satisfaction questionnaire were adopted in the investigation among 124 nurses from 28 community health service centers in Nanchong City between February to October 2016. Results The total score was (119.62 ± 15.37), at the moderate to high level. Of the eight dimensions, relationship with colleagues was scored the highest, and personal growth was the lowest. The influencing factors of the nurse's job satisfaction were found to be employment type, work positions, service duration in the community and whether they have been trained on community nursing position (all P<0.05). Conclusions The job satisfaction degree of community nurses is generally at middle level. Therefore, measures should be taken to improve the job satisfaction for the development of community nursing services.

Objective To explore the influence of continuity care model on how to deal with medical waste of insulin injections among domestic patients Methods Consecutive diabetes patients using the home insulin injections were recruited as the the members of our Diabetes Education Classfrom September 2014 to March 2015. The home medical waste disposal was brought into the content of diabetes education;problems were intervened through the continuity care model. Using the questionnaire survey to investigate the cognitive and disposal of patients on medical waste, to compare the difference of results of questionnaire before and after the intervention. Results The cognitive rate of medical waste after intervention was 97.70%(212/217),while the cognitive rate before intervention was 3.69%(8/217), the difference was significant (χ2=383.63,P<0.01). Disposal of diabetes patients using the home insulin injections had improved after the continuity care, the rate of dealing with medical waste according to specification after the intervention was 91.24% (198/217),while the rate before the intervention was 3.23%(7/217), the difference was significant (χ2=337.26,P<0.01). Conclusions The continuity care model standardized the behavior of patients for disposal of medical waste, reduced the environment pollution of medical waste and decreased the spread of disease.