Exosomes are vesicles of endocytic origin released from different cell types under both normal and pathological conditions.Proteomics is an emerging discipline for studying composition and mechanics and interac-tion of proteins with regard to disease occurrence、cellular metabolism,etc.Progress of research on exosomes from tumor cell at proteomics technology platforms in aspect of clarifying mechanisms of shaping and sustaining micro-enviroment of tumor growth and survival and metastasis, induce tumor proliferation, Immunosuppressive effects or immune evasion, and discovering biomarker, makes a promising prospect of exsomes in clinical diagnosis and treatment.

To explore the clinical efficacies of narrow-band ultraviolet B (NB-UVB) for atopic dermatitis (AD).Thirty AD patients (observation group) and 30 homochromous healthy volunteers (control group) were compared.The post-treatment scores of Scoring AD (SCORAD) and visual analogue scale (VAS) [(29.4 ± 9.5) & (3.2 ± 1.1) points] were lower significantly those pre-treatment [(60.7 ± 11.4) & 6.3 ± 1.6) points] (P ＜ 0.01).The pre and post-treatment serum levels of 25 (OH) D3 and 1,25 (OH)2D3 were detected with enzyme-linked immunosorbent assay (ELISA).The post-treatment serum levels of 25 (OH) D3 and 1,25 (OH) 2 D3 [(75.36 ± 23.28) mmol/L & (149.28 ± 45.56) nmol/L] were significantly higher than those pre-treatment in observation group [(36.24 ± 18.48) mmol/L & (97.68 ± 32.88)nmol/L] (P ＜0.01).There were no significant differences from those in group control[(69.84 ± 20.64) mmol/L & (147.36 ± 43.68) nmol/L] (P ＞ 0.05).And the pre-treatment serum levels of 25 (OH) D3 and 1,25 (OH)2D3 were all significantly lower in observation group than those in control group (P ＜ 0.01).Thus NB-UVB therapy is both safe and effective for AD and it can boost significantly the serum level of vitamin D3.

Objective To investigate the characteristics of cognitive function impairment in patients with epilepsy. Methods Forty-five epilepsy patients and twenty age-,gender-,and education-matched healthy controls were submitted to a wide neuropsychological battery,including Raven's standard progressive matrices,auditory verbal memory test,logical memory test,visual memory test,Stroop color words test,trail making test,verbal fluency test,Boston naming test,math and complex figure test,etc. Results The epilepsy patients performed significantly worse than the controls in the verbal delayed memory,verbal short memory,cued recall,immediated recall of logical memory test,visual spatial memory,attention,information processing speed test,word finding difficulty and logical reasoning.Conclusion There exists impairment in various cognitive domains in patients with epilepsy,and the main impaired areas are memory,executive functions and logical reasoning.

Health reconstruction is an important part of post-earthquake reconstruction task. To do a better job in health reconstruction after a disaster, we should, based on previous experience and the characteristics of different periods, properly utilize the health resources for the disaster relief and exercise long-term management. And in a long run, we should, taking into consideration of the future health demands, plan the health reconstruction in the disaster areas. Emphasis should be made on the personnel training for epidemic prevention. Efforts should be made to fully recover the health system in the disaster area and the recovered health system should be better than that before disaster.

Objective:To observe the effect of Schisandra Chinensis Baill distilled by ethanol on energy metabolism in brain of repeated cerebral ischemia-reperfusion model mice.Method:The mice were randomly divided into six groups,respectively irrigating the groups of Nimodipine suspension,ginkgo leaf suspension,and large and small doses of Schisandra Chinensis Baill distilled by ethanol,sham group(blank) and the model group for the same volume of saline,once a day,15 days for delivery.Model mice of cerebral ischemia-reperfusion injury was established by bilateral common carotid artery blocking.48 hours after operation,the rats were decapitated,weight half of the brain and put 9 times saline into it,then the level of LD,vitality of LDH,AchE and ATP were measured.Results:Compared with model group,small doses of Schisandra Chinensis Baill distilled by ethanol could markedly reduce the content of LD of mice of repeated cerebral ischemia-reperfusion,large doses of Schisandra Chinensis Baill distilled by ethanol could notably improve the vitality of LDH;large and small doses of Schisandra Chinensis Baill distilled by ethanol could both notably improve the vitality of AchE,large doses of Schisandra Chinensis Baill distilled by ethanol could notably improve the vitality of Na+-K+-ATP、Ca2+-ATP.Conclusion:Schisandra Chinensis Baill distilled by ethanol could markedly improve the energy metabolism of the model of repeated cerebral ischemia-reperfusion of mice.

The degradation kinetics of chlorogenic acid (5-CQA), cryptochlorogenic acid (4-CQA), and neochlorogenic acid (3-CQA) in aqueous solution at 37 degrees C and different pH values (7.05, 7.96, 9.25) were investigated in the present work. The results indicated that 3-, 4- and 5-CQA tended to remain stable in acidic pH circumstance, and unstable in neutral and alkaline pH circumstance. With the increase of the alkalinity, the degradation of 3-, 4- and 5-CQA was increased leading to a less amount of total CQA and was satisfactorily described by the Weibull equation. Meanwhile, caffeic acid was not detected after the degradation of CQA. Moreover, the degradation of 3-CQA and 5-CQA tended to be converted to 4-CQA, and the degradation of 4-CQA tended to be converted to 3-CQA rather than 5-CQA. The comparison of the degradation kinetics parameters of 3-, 4- and 5-CQA at neutral and alkaline pH values showed that the orders of the rate constant (k) values were 4-CQA > 3-CQA > 5-CQA, while the orders of the degradation half life (t½) values were 4-CQA < 3-CQA < 5-CQA, indicating the orders of the stabilities of 3-, 4- and 5-CQA at 37 degrees C and neutral and alkaline pH values were 4-CQA < 3-CQA < 5-CQA.
Caffeic Acids ; Chlorogenic Acid ; analogs & derivatives ; chemistry ; Hydrogen-Ion Concentration ; Kinetics

OBJECTIVE:To systematically evaluate the efficacy and safety of cetuximab in the adjuvant chemotherapy treat-ment of advanced non-small cell lung cancer(NSCLC),and to provide evidence-based reference for clinical treatment. METH-ODS:Retrieved from Cochrane Library,PubMed,CJFD and Wanfang database,randomized controlled trials (RCT) about cetux-imab adjuvant chemotherapy (test group) versus single chemotherapy (control group) in the treatment of advanced NSCLC were collected,and Meta-analysis was performed by using Rev Man 5.2 statistical software after extracting data and evaluating quality. RESULTS:A total of 8 RCT were included,involving 2 367 patients. Results of Meta-analysis showed survival rate in 1 year [OR=1.33,95%CI(1.08,1.64),P=0.006],partial remission rate [OR=1.48,95%CI(1.23,1.78),P<0.001],total effective rate [OR=1.34,95%CI(1.19,1.51),P<0.001],incidence of leucopenia [OR=1.50,95%CI(1.23,1.83),P<0.001],incidence of rash [OR=53.26,95%CI(13.09,216.65),P<0.001] and incidence of infusion reactions [OR=3.72,95%CI(1.86,7.42),P<0.001] in test group were significantly higher than those of control group,there were significant differences in 2 groups. However,there were no significant differences in the complete remission rate [OR=1.57,95%CI(0.91,2.70),P=0.11] and incidences of other ADR(P>0.05). CONCLUSIONS:Cetuximab has good efficacy in the adjuvant chemotherapy treatment of advanced NSCLC,however,the incidence of ADR should be prevented in clinic. Duo to the methodology limit of included studies,large-scale and high quality RCT are required for further validation of the conclusions.

Objective: To investigate the relationship between score of Uchida-Kraepelin test, achievement and stability of emotion Method: 353 cadets received Uchida-Kraepelin test and EPQ, their academic achievement and flying performance were also recorded Results: According to Uchida-Kraepelin test, those with high ability and low disorder had better flying performance than those with low ability and high disorder (t=2 23, P

Objective To investigate the changes of dopaminergic(DA) neurons in substantia nigra compact of the aged rats.Methods Using RT-PCR,Western blot,immunohistochemistry and electron microscope to detect the levels of TH mRNA and TH protein,the number and the ultrastructure of TH-positive neurons in the substantia nigra compact of the aged rats(≥24 months),and comparing those with adult rats(4～5 months).Results The level of TH mRNA(0.66?0.12) in substantia nigra compact of the aged rats was much lower than that in the adult rats(1.09?0.08)(P0.05).However,the number of TH-positive neurons in the caudal of substantia nigra compact of the aged rats was much lower than that of the adult rats(P

Objective To explore the elimination effect of radix Pulsatillae extracts on R plasmid of pseudomonas aeruginosa.Methods R plasmids of pseudomonas aeruginosa were isolated from the sputum of elderly patients suffering from respiratory infection,and the elimination test of radix Pulsatillae extracts on these plasmids was performed in vitro and in vivo.Results Being treated with the extracts for 24,48 and 72 h in vitro,the elimination rate of R plasmids was 0,1.8 %and 3.4 %respectively,while in SDS(sodium dode canesulphonate)control group the elimination rate was 0,0.8 %and 1.6 %respectively.Being treated with the extracts for 24,48 and 72 h in vivo,the elimination rate was 0,2 %and 12.8 %respectively,and 0 in the control group.Conclusions Both in vitro and in vivo,radix Pulsatillae extracts have an elimination effect on R plasmid of pseudomonas aeruginosa,and the effect in vivo is stronger than that in vitro.