Objective: To study the chemical constituents in the roots of Goniothalamus cheliensis. Methods: Silica gel, ODS, and Sephadex LH-20 column chromatographic techniques were used to isolate and purify the chemical constituents and their structures were elucidated by spectral analyses. Results: Seven compounds were isolated and identified as acetylgoniofupyrone A (1), protocatechuic acid (2), pinocembrin (3), pinoresinol (4), goniodiol (5), 8-epi-goniotriol (6), and cardiobutanolide (7). Conclusion: Compound 1 is a new goniofupyrone-type of styryllactone, named acetylgoniofupyrone A.

OBJECTIVE:To observe analgesic efficacy of oxycodone acetaminophen after posterior lumbar fusion,and to opti-mize spinal postoperative analgesia plan. METHODS:In retrospective study,120 patients underwent posterior lumbar fusion were selected from the Affiliated People's Hospital of Hubei Medical College during Jun. 2013-Jun. 2014,and then divided into observa-tion group and control group according to therapy plan,with 60 cases in each group. Observation group was given oxycodone acet-aminophen 330 mg orally 6 h after surgery,and then 330 mg orally on 1st,2nd day after surgery,tid. Control group was given celecoxib 200 mg 6 h after surgery,and then 200 mg on 1st,2nd day after surgery,bid. Postoperative analgesic efficacy (VAS score) at resting state and active state,postoperative gastrointestinal motility recovery (the time to flatus) and the occurrence of ADR were observed in 2 groups after surgery. RESULTS:Whether at the resting state or the active state 24,48,72,120 h after surgery,VAS score of observation group was significantly lower than that of control group,with statistical significance(P<0.05). There was no statistical significance in the time to flatus and the incidence of ADR between 2 groups(P>0.05). CONCLUSIONS:Oral administration of oxycodone acetaminophen possesses good effect to relieve spinal postoperative pain,showing fast and safe analgesic efficacy.

OBJECTIVE: To investigate the clinical features of primary biliary cirrhosis (PBC) and its treatment by integrated traditional Chinese and western medicine. METHODS: 16 PBC patients were observed. Ursodeoxycholic acid (UDCA) was used in the dose of 13 to 15 mg.kg(-1).d(-1), with some traditional Chinese herb prescription. Results (1) The proportion of women to men was 15:1, the mean age was 52.5 years. AMA-M2 was positive in 14 subjects (87.5%). Biliary tract enzymes and ESR were elevated in all subjects. The ratio of hypercholesterolemia (CHOL) and abnormality in IgM was high (62.5%). Fatigue, pruritus, arthralgia, jaundice, splenomegaly were noted in more than half cases. Every patient had one to four complications. (2) ALP, GGT and Glb declined evidently after 3 months' treatment by western medicine associated with traditional Chinese medicine, and declined markedly after 12 months' treatment (P<0.05 respectively). TBIL and CHOL declined gradually during the treatment course. Symptoms and signs were lightened. CONCLUSION: PBC has complicated and especial clinical features. UDCA therapy is effective in PBC, while traditional Chinese medicine has extraordinary effect in treating the symptoms and signs.

The property theory of Chinese herbal medicine (CHM) is regarded as the core and basic of Chinese medical theory, however, the underlying mechanism of the properties in CHMs remains unclear, which impedes a barrier for the modernization of Chinese herbal medicine. The properties of CHM are often categorized into cold and heat according to the theory of Chinese medicine, which are essential to guide the clinical application of CHMs. There is an urgent demand to build a cold/heat property classification model to facilitate the property theory of Chinese herbal medicine, as well as to clarify the controversial properties of some herbs. Based on previous studies on the cold/heat properties of CHM, in this paper, we described a novel strategy on building a cold/heat property classification model based on herbal bio-effect. The interdisciplinary cooperation of systems biology, pharmacological network, and pattern recognition technique might lighten the study on cold/heat property theory, provide a scientific model for determination the cold/heat property of herbal medicines, and a new strategy for expanding the Chinese herbal medicine resources as well.
Drugs, Chinese Herbal ; chemistry ; therapeutic use ; Herbal Medicine ; Humans ; Phytotherapy ; Plants, Medicinal ; chemistry

Objective To explore the relationship between transforming growth factor-β1 (TGF-β1) and obesity-related glomerulopathy (ORG),and to analyze the possible mechanism for ORG and the new approach to its treatment.Methods Based on their body weight,30 SD rats were randomly divided into 2 groups : the normal control group (15 rats) fed with common food and the ORG model group (15 rats) fed with fat-enriched diets.The rats were sacrificed at the end of the 8th week,and their kidneys were taken out.Immunohistochemistry was used to detect TGF-β1 protein expression.Real time (RT)-polymerase chain reaction (PCR) was used to extract and detect the expression of TGF-β1 mRNA,and Western blot was applied to examine the expression of TGF-β1 protein.The findings were analyzed by using SPSS 13.0 software.Results Compared with the control group, qualitative TGF-β1 expression in ORG model group were significantly increased detected by immunohistochemistry mainly in renal tubules and interstitium.The average absorbance value of the control group and the model ORG group was 0.040-0.013,0.171 ± 0.084, respectively.The difference was statistically significant(P ＜ 0.05).The expression of TGF-β1 mRNA detected by RT-PCR was also increased compared with that of the control group(4.4 vs 0.6).The difference was statistically significant(P ＜ 0.05).The protein expression of TGF-β 1 examined by Western blot showed that it was more than that in the control group(4.3 vs 0.4).The difference between the control group and ORG model group was statistically significant(P =0.002).Conclusions The expression of TGF-β 1 in kidneys of ORG model rats increased, which not only indicates it can participate in ORG's occurrence and development, but also provide the basis to find out the mechanism and the approach to treatment.

Objective To comprehensively evaluate the effectiveness of health education interventions on endemic fluorosis for Chinese students and housewives,and to provide a basis for prevention and control of the disease.Methods A computerized literature search was carried out in CBM(Chinese Biomedical Database),CNKI (Chinese National Knowledge Infrastructure),Wan-fang,VIP,Pub-Med,Web of Knowledge and EBSCO to collect articles published at home and abroad between 1994-2012 concerning the effectiveness of health education interventions on endemic fluorosis for Chinese students and housewives.The study was a self-controlled intervention study.The quality of literature was evaluated using the standards of Cochrane and EPOC.Meta-analysis was performed to assess the knowledge about fluorosis prevention using Rev-Man 5.0.Homogeneous data(P ＞ 0.05) was analyzed using a fixed effects model,and heterogeneous data (P≤ 0.05) was analyzed using a random effects model.Results A total of 146 Chinese research papers and 192 English research papers were retrieved.By screening,35 Chinese research papers meet the standards.After reading the full text,6 papers were selected.English literature was not found.According to EPOC criteria all the six literatures included were grade B.Because heterogeneity of the literatures was higher,knowledge point was stratify analyzed according to the study subjects.The intervention effect of students was higher than that of the housewives.After the intervention,the fluorosis disease prevention knowledge awareness of schoolchildren and housewives were increased by 28％ and 26％.Conclusions Health education has a good effect in improving the knowledge of fluorosis for Chinese students and housewives.

Objective To support a reasonable and standard TCM treatment principle for advanced non-small-cell lung cancer (NSCLC) by exploring TCM syndrome features in NSCLC patients with factor analysis. Methods 105 cases of advanced NSCLC patients,who were admitted to the hospital after 1st January,1998 from 18 to 75 years old,were studied retrospectively with factor analysis of the data. Results According to analysis and synthesis of tongue,pulse and symptoms,the deficiency of qi and yin (37.14%) and the stagnation of phlegm and blood stasis (39.05%) were the major syndromes when advanced NSCLC was diagnosed. Conclusion One of TCM intervention treatment principles for NSCLC,which includes invigorating qi and yin,promoting blood circulation and removing blood stasis,regulating the flow of qi and resolving phlegm,is proposed to instruct the application of herbs,Chinese traditional patent medicines or injections in integration treatment and stage treatment for improving quality of life and elongating survival period.

Objective:To investigate the effects of Astragalus on the the apoptosis of cultured human peritoneal mesothelium induced by peritoneal dialysis solution(PDS). Methods:Using RPM1640 culture medium as control,PDS group(commercial PDS containing 1.5% glucose without Astragalus),Astragalus group 1(commercial PDS containing 1.5% glucose and 1% Astragalus) and Astragalus group 2(commercial PDS containing 1.5% glucose and 2% Astragalus) were tested to explore the apoptosis of cultured human peritoneal mesothelial cell in each group.The activity of caspase-3,the terminal deoxynucleotidyl transferase(TdT) mediated dUTP nick end labeling(TUNEL) and flow cytometry analysis were used in apoptosis analysis in this study. Results: The activity of caspase-3 in the control group was defined as 1,the relative activity of caspase-3 in PDS group、 Astragalus group 1 and Astragalus group 2 were 3.26?0.91,(1.87?)0.43 and 1.67?0.32 respectively.The activity of caspase-3 in PDS group was significantly higher than those in the Astragalus group 1 and Astragalus group 2(P

To explore the effects of dietary conjugated linoleic acid(CLA) on body fat accumulation in male Wistar rats. The obese rat model was established by feeding high lipid forage. The rats were fed a semi-purified diet containing 0.5,1.0,3.0g?kg -1 CLA for 36 days. Low dose group, middle dose group and high dose group all could remarkably reduce the gain of body weight. Low dose group could reduce the body adipose, middle and high dose group could remarkably decrease the body adipose. There were significant differences in the fat and protein contents in the rats thigh between the control group and the group fed high dose CLA. These results suggested that CLA can reduce the body fat in obese Wistar rats.

Objective: To investigate the effect of Astragalus on peritoneal macrophages function in stable continuous ambulatory peritoneal dialysis(CAPD) patients. Methods: 28 stable CAPD patients were included in this study.All the patients were treated with peritoneal dialysis fluid containing Astragalus(20 ml/2 L) in vivo for one week.Peritoneal macrophages function such as phagocytosis ratio、bactericidal ratio、excretion of cytokines(TNF-? and NO) and mitochondrial dehydrogenases activity(MTT assay) were examined ex vivo just before and after the treatment. In vitro,peritoneal macrophages isolated from effluent were incubated with RPMI 1640、CDF or CDF containing various concentrations of Astragalus. Then peritoneal macrophages function mentioned above were examined again and compared. Results:No significant difference was found in TNF-? level in effluent between before and after the treatment (100?63)ng/L vs (116?60)ng/L, (P=0.192). While comparing with pre-treatment, peritoneal macrophages function was improved significantly after the addition of Astragalus into dialysate (phagocytosis ratio: (34.8?12.7)% vs (43.4?9.3)%,P0.05).The results in these two groups were significantly lower than those in Astragalus groups(P0.05). While significant decreasing in NO level and mitochondrial dehydrogenases activity were observed in 8% Astragalus groups as compared with those in 2% Astragalus group(P