Objective To analyse correlated risk factors of evolving cerebral infarction(ECI).Methods The follow data was recorded in the acute cerebral infarction (ACI)cases when they were admitted :sex,age,systolic blood pressure(SBP),diastolic blood pressure(DBP),history of disease (diabetes mellitus,hypertension,hyperlipidaemia,cerebral infarction,hyperuricemia or smoking).The following examinations or determinations were done,including:white blood cell count (WBC),platelet count (PLT),blood gluconate(GLU),PT-INR,fibrinogen(FG),TT-INR,APTT-INR,C-reactive protein(CRP),D-dimer(D-D),products of fibrin degradation(FDP),anti-thrombin Ⅲ(ATⅢ),apolipoprotein A(apoA),apolipoprotein B(apoB),blood urea nitrogen(BUN),creatinine(CR),triglyceride (TG),high density lipoprotein (HDL),low density lipoprotein (LDL),very low density lipoprotein (VLDL),GOT,lactate dehydrogenase(LDH),creatine phosphokinase (CPK),MB isoforms of creatine phosphokinase (CPK-MB),? hydroxybutyrate dehydrogenase (?-HBDH),troponin I(TnI),cerebral CT or MRI and carotid ultrasonography.The patients were divided into two groups,ECI and completed cerebral infarction (CCI),according whether neurons function scale deteriorated.When cases of both groups exceeded 50,we took statistic test by SPSS10.0 statistic software.Results 8 of the above 46 markers had significant defference between the two groups,including CRP,WBC,apoB,GLU,LDH,CPK-MB,?-HBDH and DBP.ECI was more common in patients who had lower DBP or higher CRP,WBC,apoB,GLU,LDH,CPK-MB,?-HBDH when they were admitted.Conclusion The increase of CRP,WBC,apoB,GLU,LDH,CPK-MB,?-HBDH or decrease of DBP when patients are admitted can be predictive markers of ECI.

To investigate the short term anti HBV efficacy of foscarnet sodium, sixty seven patients with various types of chronic hepatitis B were randomly divided into two groups. The experimental group (47 cases) was assigned to receive foscarnet sodium 3 0g by intravenous infusion twice daily in addition to general liver protective medicine for 15 days. The control group (20 cases) was treated with regular liver protective medicine only. The quantity of HBV DNA was measured with equivalent competitive PCR combining with DNA hybridization quantitative detection technique before and after treatment (once a week). The HBV markers and liver functions were also tested before and after treatment. In antiviral therapy group, the patients with different types of hepatitis B had their liver functions improved. HBV DNA in 13 patients became negative by PCR. Two of HBeAg positive patients became sero converted. Foscarnet sodium can inhibit HBV efficiently and quickly. The replication of HBV DNA can be greatly suppressed in the first week but without significant change in the second week in some cases. Foscarnet can be one of the drugs of choice in a combined therapy or as the initial drug in a sequential therapeutic regime.

AIM: To explore the effects of advanced gly cosyla tion end products (AGEs) on connective tissue growth factor (CTGF) mRNA expressi on and extracellular matrix (ECM) synthesis in cultured rat renal mesangial cel ls. METHODS: Rat renal mesangial cells were cultured in vitro un der standard conditions. The content of fibronectin (FN) and type Ⅳ collagen (ColⅣ ) were determined with ELISA after stimulation by AGE-BSA and BSA, respectively. CTGF mRNA expression was assessed by RT-PCR. RESULTS: CTGF mRNA was significantly increased by AGE-BSA in cul tured mesangial cells in a time-and dose-dependent manner compared with BSA cont rol (P

Objective To study the effect of the complexus of radix salviae miltiorrhizae and lignstrazin (DCs) on the learning and memory impairment in Alzheimer disease rats. Methods Rats were randomly divided in- to 5 groups(10 rats/group):normal eontrol group, Alzheimer disease model group toxified with AlCl3 and D-gal,3 groups toxified with AICl3 and D-gal,whieh were treated with the different drugs including piracetam and DCs1,2. After 80 days,step-through and morris water maze experiments were used to detect the learning and memory ability. Results Compared with Alzheimer disease model group,DCs obviously improved the learing and memory ability of Alzheimer disease rats,it is increased significantly that active reaction, and the number of active avoidance, improve achievement, evasion failed to reduce the number of passive evade significantly shorten the delay time in Des-treated group. Conclusion DCs markedly improved the learing and memory ability of Alzheimer disease rats.

Objective To investigate the correlation between Framingham stroke risk profile(FSRP) and vascular cognitive impairment in stroke-free patients with cerebrovascular risk factors.Methods One hundred and eighty-four stroke-free subjects,selected from Zhejiang hospital,were divided into low risk group (56 subjects),moderate risk group (70 subjects) and high risk group (58 subjects) according to their FSRP score,and their cognitive function including memory ability,attention,executive function and language ability were assessed by Montreal cognitive assessment (MoCA),auditory verbal learning test (AVLT),digit symbol test,trail making test(TMT),digit span and verbal fluency test.Results The total MoCA scores which were (7.2±4.6),(13.8±3.9),(29.6± 12.7) respectively,AVLT-delay recall scores which were(8.2± 1.6),(6.7± 1.4),(5.9± 1.5) respectively,and digit symbol test score which were(34.7±9.3),(32.6± 16.4),(29.7± 13.6) respectively in low,intermediate and high risk groups,decreased with the increasing risk of stroke(P＜0.05).The elapsed time in TMT-B which were (115.2 ±36.9) s,(147.6±44.8) s,(173.9±58.5) s respectively in low,intermediate and high risk groups,prolonged with the increasing risk of stroke (P＜0.05).FSRP was associated with cognitive function,but inversely related to MoCA,AVLT-delay recall,digit symbol test,TMT-B and digit span fall back (P＜0.05),but positively related to consuming time in TMT-B (P＜ 0.05).Multivariate regression analysis showed that advanced age,hypertension,diabetes and smoking were the risk factors for vascular cognitive impairment(P＜0.05).Conclusion Advanced age,smoking,hypertension and diabetes are the most important in vascular risk factors for cognitive impairment.Vascular risk factors can damage cognitive function with the increased risk of stroke,among which delayed recall and executive function are the main affected cognitive area.

Objective To investigate the effects of advanced glycation end products(AGEs) on expression of peroxisome proliferator-activated receptor-?(PPAR?) mRNA in rat renal cortex.Methods Normal rats were given tail vein injection with either AGE-modified rat serum protein(AGEs)or AGE-RSP followed by intraperitoneal injection of AG(AGEs+AG)or native rat serum protein(native RSP).Normal rats without any treatment were as controls(Control).PPAR-? mRNA expression was analysed by reverse transcriptase-polymerase chain reaction(RT-PCR).Results PPAR-? mRNA expressed in all rat kidney cortex.There was a decrease for PPAR-? in mRNA levels in the renal cortex of AGEs-treated rats(P

Objective To explore the effect of boiled fenugreek seeds on renal matrix metalloproteinase-2(MMP-2) activity in diabetic rats.Methods The model of diabetes was built with STZ in rats.The model rats were randomly divided into diabetes control groups (DM ) (n=10) and fenugreek seeds groups(FN) (n=10,and while normal control group (N) (n=10)rats was used.Diabetic rats were treated with fenugreek seeds for 12 weeks,the renal morphology and MMP-2 activity were observed in three groups .Results After diabetic rats were treated with fenugreek seeds for 12 weeks,optical microscopic examination indicated that the glomerular structure in N group was normal,the glomerular lesions in rats of DM groups were seriously and the pathologic changes of glomerular in rats of FN groups were alleviated significantly.Immunohistochemical results showed that the Col Ⅳ expression in glomerular ECM was increased in DM group compared with N group,and was decreased in FN group.The activity of MMP-2 was increased in FN group (1.41?0.18) compared with DM group (1.05?0.19) (P

Objective To review the epidemiologic features of norovirus infection outbreaks in Zhejiang province during 2004-2014.Methods Epidemiological data of norovirus infection outbreaks in Zhejiang province from January 2004 to February 2014 were collected from the Emergency Public Reporting System in Zhejiang Provincial CDC.The distribution of time,area,population,route of transmission and genotype of norovirus were analyzed.Results There were 16 outbreaks of norovirus infections with 2 037 cases during 2004-2014 in Zhejiang province.Eleven outbreaks occurred during February and April,and 13 outbreaks occurred in schools.The outbreaks in schools mainly involved students aged 15-20 years,while other outbreaks took place mainly in the young and middle-aged population.The sex ratio of male to female was 1.05 ∶ 1.Among 16 outbreaks,10 were induced by norovirus G Ⅱ infections,3 were induced by norovirus G Ⅰ infections and 3 were induced by norovirus G Ⅰ and G Ⅱ infections.Fourteen outbreaks were caused by water pollution.Conclusion Outbreak of norovirus infection usually occurs in schools during winter and spring in Zhejiang province,and the epidemic of disease is mainly associated with polluted water.

Objective To in ve stigate the effects of advanced glycation end products (AGEs) on matrix metallop roteinase-2 (MMP-2) activity and its mRNA expression in renal cortex of rats. Methods Diabetic model of rats was induced by s treptozotocin. AGEs were prepared by incubation of rat serum protein with 0.5 mo l/L glucose. AGEs was administered intravenously to normal rats (AGEs group), an d native rat serum protein was given as negative control (negative group) and no rmal rats without treatment were as control (control group). AGEs content in ren al cortex and serum was quantified by ELISA, MMP-2 and TIMP-2 mRNA expressions were examined by RT-PCR and MMP-2 activity was measured by zymography. Results AGEs content increased significantly, MMP-2 mRNA expression descended and TIMP-2 mRNA expression ascended in renal cortex o f diabetic rats (all P

Objective: To observe the effect of oxymatrine (OM) on the expression of HBV DNA in HepG2.2.15 cells and to investigate the anti-HBV mechanism of OM. Methods: The level of HBV DNA in HepG2.2.15 cells incubated with different concentrations of OM was quantified by equivalent competitive PCR combining with DNA hybridization quantitative detection technique (PCR-ELISA). The in vitro anti-viral effect of OM was evaluated by calculating the inhibiting rate. Results: OM inhibited the expression of HBV in HepG2.2.15 cells. The inhibiting rate increased with the drug concentration. The stable concentration of OM in medium was important in keeping the inhibiting rate. Conclusion: OM can inhibit the synthesis of HBV directly at the level of HBV DNA replication.