Objective To discuss the experience of self -perceived burden(SPB)in AIDS patients and to provide evidence for promoting physical and mental health.Methods Interviews were conducted among 10 AIDS patients,and the data were analyzed with the Colaizzi 's method.Results 3 themes from the experience of self -perceived burden in AIDS patients were identified:self -accusation and remorse,fear and insecurity,loss of dignity. Conclusion The research results showed feelings,difficulties and needs of patients in the process of disease.Nurses should think about the influence of SPB during the nursing intervention and evaluation.Nurses should alleviate the problems faced by patients in order to increase the health level,reduce the psychological pressure and increase the medication compliance of the patients in the long -term treatment.It can be used in clinical nursing.

Objective To explore the clinical outcomes of vestibular rehabilitation therapy(VRT) in the treat‐ment of patients with balance disorders .Methods Seventy -six patients were diagnosed with vertigo based on the principle of single and double side targeted rehabilitation ,and were divided into four groups randomly .Group A of 29 cases received unilateral simple drug treatment only ,group B of 29 cases received unilateral drug treatmeat com‐bined with vestibular rehabilitation therapy ;bilateral simple drug treatment for the 9 cases in group C ,group D of 9 cases accepted bilateral drug treatment combined with vestibular rehabilitation therapy .Group A and C were the control groups while B and D were experimental groups .All cases were retrospectively analyzed to compare vestibu‐lar rehabilitation therapy combined with drugs treatment with the simple drug treatment group .Results The effects in all patients were better after treatment .The VSI scores four weeks after treatment in four groups were signifi‐cantly lower than that of before treatment .The BBS score after treatment of experimental groups were significantly higher than those of in the control groups .The Fukuda step experiment effects were better than the control group . All the above comparisons had a statistical significance(P< 0 .01) .Conclusion The clinical efficacy of normative vestibular rehabilitation exercise is satisfactory .

Objective To study the effects of acute hypervolemi c hemodilution (AHH) on the coagulation and cardiac function in elderly surgical p atients. Method Sixty surgical patients, aged 60 to70, ASA Ⅰ~ Ⅱ, were randomly divided into three groups (n=20 for each group). 6% HES 50 0~1000ml was infused in a rate of 20ml/min during vasodilation with isoflurane i nhalation (group 1), nitroglycerin (group 2), or nicardipine (group3) with the a id of an intravenous injection pump. The blood coagulation and cardiac functions were observed during hemodilution. Results No significant diff erence was found among the three groups on cardiac function and coagulation func tion during 500ml 6% HES infusion. When the volume of infusion was over 1000ml, the parameters of cardiac function increased and those of coagulation decrease d significantly (P

Objective To examine the effects of different n-6/n-3 polyunsaturated fatty acid(PUFA) ratio in gap junctional intercellular communication (GJIC) in breast cancer cell lines. Methods After MCF-7(ER + )and MDA-MB-231(ER - )cells were treated respectively with n-6/n-3 polyunsaturated fatty acid ratio (1-10), Ki-67 expression in nuclear by immunocytochemistry, connexin 26 and 43 expression in cytoplasm and membrane by Western blotting, and GJIC by scrape-loading dye transfer method were performed. Results Immunocytochemistry showed that pure n-6 and 10∶1 n-6/n-3 increased Ki-67 expression (P

Objective To study the effects of different n-6/n-3 polyunsaturated fatty acid ratio on the lipid metabolic gene expression and MAPK activity in breast cancer cell lines. Methods MCF-7 and MDA-MB-231 cells were treated with n-6 polyunsaturated fatty acid, or n-3 polyunsaturated fatty acid, or the mixture of n-6/n-3 polyunsaturated fatty acid at ratio of 1∶1, 5∶1, 10∶1. The cell proliferation capacity was detected by MTT, the expression of lipid metabolic gene (COX-2, 5-LOX, PPAR? and PPAR?) were assayed by RT-PCR, MAPK phosphorylation levels were detected by Western blotting. Results COX-2 mRNA expression was not found in MCF-7 cells. As compared to normal control group, pure n-6 and 10∶1 n-6/n-3 promoted cell proliferation, up-regulated the expression of COX-2, 5-LOX, PPAR? and PPAR? mRNA , increased MAPK phosphorylation levels in MCF-7 and MDA-MB-231 cells (P

Objective To observe the effects of soybean isoflavone in attenuating trans fatty acids-induced athero-sclerosis in rats .Methods The rats were randomly divided into normal diet group、trans fatty acids group、soybean isoflavone group and trans fatty acids +soybean isoflavone group .Research kit was used to test the amounts of TG , HDL,LDL and IL-6,TNF-αin rats'blood.Bovine aortic endothelial cells were cultured and divided into control group,trans fatty acids group and trans fatty acids group +soybean isoflavone group .Western blot was performed to test the NF-κB phosphorylation ,as well as to determine ICAM-1 and VCAM-1 protein contents in bovine aortic endo-thelial cells.Results Compared with normal diet group ,the amounts of TG,LDL,IL-6,TNF-αincreased evidently (P<0.05), whereas the amounts of HDL decreased evidently (P<0.05) in the rats'blood of trans fatty acids group . After the soybean isoflavone intervened ,the amounts of TG,LDL,IL-6,TNF-αall decreased (P<0.01), meanwhile the amounts of HDL increased significantly (P<0.01).Soybean isoflavone inhibited NF-κB phosphorylation induced by trans fatty acids ( P<0.01) .The high expression of ICAM-1 and VCAM-1 induced by trans fatty acids was de-creased by soybean isoflavone when comparing with those in the control group ( P<0.01 ) .Conclusions Soybean isoflavone has a role in attenuating trans fatty acids trans fatty acids-induced atherosclerosis in rats .

ObjectiveTo compare the effect of patient-controlled epidural analgesia (PCEA) and patient-controlled intravenous analgesia (PCIA) on pulmonary function in post-thoracotomy patients.Methods33 ASA Ⅰ～Ⅱ patients undergoing selective esophagectomy were randomly divided into the PCEA group (n=16, treated with morphine plus bupivacaine) and PCIA group (n=17, treated with morphine plus droperidol) for 3 days postoperatively. Pulmonary function indices including respiratory rate (RR), tidal volume (Vt), vital capacity (Vc) and pulse oximetry (SpO2) were recorded before operation and on the first 2 days after operation. Pain scores with visual analogue scale (VAS) at rest, deep breathing and with cough, and adverse effects were also recorded.ResultsRR increased, Vt , Vc and SpO2 decreased markedly in both groups postoperatively compared with the base line (P<0.01), but there were no significant differences between two groups. VAS scores were much lower in PCEA group, especially, when the patient was at deep breathing or during coughing (P<0.001).ConclusionPCEA is superior to PCIA in pain relief, but contributes no more than PCIA in improving pulmonary function in post-thoracotomy patients.

ObjectiveTo compare the effects of patient-controlled epidural analgesia (PCEA) and patient-controlled intravenous analgesia (PCIA) on postoperative nausea and vomit (PONV) in gynecologic and obstetric patients. Methods56 gynecologic or obstetric patients (ASA Ⅰ～Ⅱ) scheduled for lower abdominal surgeries were randomly allocated to receive either 1 mg/ml morphine plus 0.1 mg/ml droperidol intravenously (group PCIA) or 0.1 mg/ml morphine plus 0.125% bupivacaine (group PCEA-Ⅰ) or 0.1 mg/ml morphine plus 0.1 mg/ml droperidol plus 0.125% bupivacaine (group PCEA-Ⅱ) epidurally. 4, 24, and 48 h after operation, pain scores with visual analogus scale (VAS), sedation scores with Ramesay and the incidences of nausea, vomiting, pruritus, respiratory depression were assessed. ResultsVAS scores in the two PCEA groups were much lower than that of PCIA (P<0.01). The incidences of nausea and vomiting in PCEA-Ⅱ group were significantly lower than those in PCIA group (P<0.05), incidences of other side-effects such as pruritus, respiratory depression etc. were similar between the three groups (P>0.05). ConclusionThe regimen morphine/droperidol/bupivacaine by PCEA shows superiorities in relieving pain and reducing postoperative nausea and vomiting in gynecologic and obstetric patients.

Objective　To explore the metabolism of micronutri ents related to dark adaptation of radar operators through nutritional intervent ion. Methods　A total of 34 male radar operators aged between 18 ～29 years old were randomly divided into control and experimental groups. The c ontrol group were on normal diet, and the experimental group received the supple ment of VA, Zn and Se in additional to normal diet. The experiment lasted 4 week s. The levels of serum VA, Zn and Se were measured before and after the experime nt. Results　The levels of serum VA, Zn and Se in the experime ntal group were significantly higher than that in the control group after the experiment (P<0.01). Conclusion　The supplement of VA, Zn an d Se for 4 weeks may elevate, the levels of serum VA, Zn and Se significantly (reached or surpassed normal levels) and suggests that VA, Zn and Se su pplementation may effectively enhance the dark adaptation of radar operators.