Objective: To study the effect of calcium on osteocalcin(OC) and parathyroid hormone (PTH) in rat exposed to lead. Method: Wistar rats were randomly divided into control group, lead group, low-calcium+lead group and high-calcium+lead group. The content of calcium and lead in bone, OC and PTH of rat with different treatments were detected. At the same time, RT-PCR analysis was used to determine the expression of OC and PTHr1. Results: Lead decreased the content of calcium in bone and OC. The expression of OC was inhibited, and the level of PTH and the expression of PTHr1 increased, which could be enhanced by low calcium treatment, but high calcium treatment could antagonize these effects of lead. Conclusion: Supplementation of calcium in diet could extenuate the toxicity of lead on bone in rat.

Objective To study the effect of lead on the calcium absorption and bone development in weanling rats. Methods Totally 80 weanling Wistar rats were equally divided into normal control (given with pure water and standard feed, including 1.15% calcium), lead group (given 1.0 g/L lead acetate water and standard feed), lead+low calcium group (1.0 g/L lead acetate water and feed including 0.69% calcium), and lead+high calcium group (1.0 g/L lead acetate water and feed including 1.72 % calcium). The development of rats was observed. Serum contents of osteocalcin and parathormone,and bone levels of lead and calcium in the femur were determined. The femur was examined with histological method. Another 5 Wistar rats received gastric irrigation of 10% lead acetate for 5 d, 5 more rats served as control, and then their absorption of calcium was detected with 45CaCl2. Results Lead and low calcium inhibited the development of rat remarkably, with the content of osteocalcin, the length and diameter of the femur decreased. High calcium antagonized these effects of lead. The absorption of calcium in rats was repressed by lead. Conclusion Bone depression by lead may be due to that lead inhibits the absorption of calcium in rats, and the supplement of calcium is helpful to minimize the repression.

cases of Warthin tumor in parotid gland were collected, the selection of operating style and personal experience in treatment were introduced. The results showed that most of the patients were senior man, the site of the tumors was all in parotid gland, the majority of the tumor was single nodule with exception of some patient who had a multiple nature. Tumor recurrence was found in patients with improper method. The results suggested that for patients who are suspected of Warthin tumor,careful physical examination and B Ultrosonic scan are necessary for both sides of parotid gland. Different operating style should be considered according to the differences of tumor site and number.

BACKGROUND: Palatal implant could be an effective type of anchorage that can afford long-term stress, and bone thickness and bone density of the implant placement will affect the success of the implant stability. Presently, actinoscopy commonly used in stomatological clinic cannot provide the precise information of palatal bone thickness and bone mineral density such as toroidal slide plate photograph and skull lateral projection. OBJECTIVE: To measure the thickness and density of median palatine suture, and to provide the consultation for implant anchorage using spiral CT. DESIGN, TIME AND SETTING: A measurement study was performed at the Medical Imaging Center, First Affiliated Hospital of Xinjiang Medical University from July to December in 2008 PARTICIPANTS: The sample consisted of 194 cases undergoing 64 slice CT at the Medical Imaging Center of First Affiliated Hospital of Xinjiang Medical University, including the scope of the hard palate and maxillary dentition. They were aged from 18 to 50, and divided into three groups by age, 18-30-year-old group, 31-40-year-old group, and 41-50-year-old group. METHODS: Measurement of palatal raphe area first three-dimensional reconstruction of images at up to nasion plane centerline, whichever was determined as a reference surface plane, selection one point on incisor cervix in this line, to measurement of the available palatal bone height; sagittal views were selected at 6 mm dorsally from the incisive canal. The available bone height was defined as the distance from the palatal baseline (lower cortical border of the palate) to the nasal cavity (upper cortical border of the nasal cavity). Bone mineral density can also be measured at the same site, take the average of the cortical and cancellous bone. MAIN OUTCOME MEASURES: The palatal bone thickness and bone thickness density were measured at 6 mm dorsally from the incisive canal. RESULTS: Male in the palatal bone thickness and bone mineral density were significantly higher than female (P < 0.05); there were no significant difference between Han and Uygur (P>0.05). In different age's group of men and women, the palatal bone thickness and bone mineral density showed no statistical difference (P > 0.05). Age, palatal bone thickness and palatal bone mineral density had no significant correlation. CONCLUSION: Spiral CT can be used in different parts of mandible thickness and density measurement and analysis. The palate raphe can be implant as orthodontic anchorage if it measured exactly.

Objective To investigate the chromosome karyotype of acute lymphocytic leukemia (ALL) and its correlation with the clinical feature and efficacy.Methods The chromosomes of bone marrow/peripheral blood from 110 cases of patients with ALL were prepared after 24 hours culture,and G-banding were used to analyze karyotypes.Results Among 110 patients with ALL,71 cases (64.5 ％) had clonal chromsomal normalities,39 cases (35.5 ％) had clonal chromsomal abnormalities,24 cases (21.8 ％) had chromosome structural abnormalities,11 cases (10.0 ％) had chromosome number abnormalities,3 cases (2.7 ％) had chromosome number and structure abnormalities,one case had chromosomal abnormalities complex karyotype.Efficacy in patients with ALL with t(9;22) (q34;q11) was worse than the other patients (Fisher s exact text,P =0.045).There was no significant difference on efficacy between in adult ALL associated with t(9;22) (q34;q11) and in children with ALL (Fisher's exact text,P =0.506).Conclusion Chromosome karyotype of ALL patients is random,chromosomal translocations such as t(9;22)(q34;q1 1) and t(4;11) (q21;q23) have poorer treatment outcomes.

This study is to determine the preventive effect and mechanism of targeting IL-17A on pulmonary inflammation and fibrosis after acute lung injury. Mice were treated with anti-IL-17A antibody on the day 7 and sacrificed on the day 14 after bleomycin lung injury. The pulmonary inflammatory status and the deposition of collagen were measured by HE and Sirius stains staining. The contents of hydroxyproline and collagen were measured by using commercial kits. The survival rate of mice was calculated by Kaplan-Meier methods. The inflammatory cytokines in bronchoalveolar lavage fluid were measured by ELISA and the expressions of inflammation-related molecules were detected by Western blotting assay. Targeting of IL-17A could prevent the development of lung inflammation, decrease collagen deposition and the contents of hydroxyproline, and protect against the development of pulmonary fibrosis, which together led to an increase in the animal survival. Moreover, blocking IL-17A decreased the expression ofpro-fibrotic cytokines such as IL-17A, TGF-beta1 and IL-13; increased the expression of anti-fibrotic or anti-inflammatory factors such as IFN-gamma, COX-2, 5-LOX, 15-LOX. Indeed, IL-17A antagonism suppressed the activation of pro-inflammatory p65NF-kappaB but enhanced the activation of pro-resolving p50NF-kappaB. In conclusion, that blockade of IL-17A prevents the development of pulmonary fibrosis from acute lung injury, is because blocking IL-17A may prevent acute inflammation converting to chronic inflammation.

Objective SD rat models of stomach-heat syndrome were established to further investigate the physiopathological mechanism of stomach-heat syndrome.Methods Under certain laboratory condition,criteria of stomach-heat syndrome for the animal models were set up firstly.Then decoction of Rhizoma Zingiberis was given to the rats for modeling.Two weeks after modeling,the symptoms of the rats were observed and pathological and biochemical examination was carried out.Weireqing capsules were used to confirm the success of the establishment of stomach-heat syndrome in rats.Results Two weeks after modeling,the model rats had the symptoms of thirst with preference for drinking and reddened tongue.Congestion in gastric mucosa occurred and the levels of 6-keto-PGF1?and TXB2 in the model group were higher than those in the control group.However,in model rats pretreated with Weireqing,the symptoms and signs of stomach-heat syndrome and histological changes were relieved.Conclusion Under the controlled laboratory condition,feeding rats with decoction of Radix Zingiberis can be successfully used to establish animal model of human stomach-heat syndrome .

OBJECTIVE To investigate the disinfectant-resistant gene qacE△1-sul1 in Pseudomonas aeruginosa isolated from burned patients. METHODS GNS-448 and K-B tests were performed to detect the susceptibility of 19 kinds of antimicrobial agents against these strains. Genotype was analyzed by polymerase chain reaction(PCR) and verified by DNA sequencing. RESULTS The 32 strains isolated were all resistant to ampicillin,cefuroxime,cefoxitin,SMZ/TPM. The sensitive rates to amikacin and gentamicin were 68.0%,and 46.9%,respectively,the resistant rates to imipenem and meropenem were 68.8% and 59.4%,respectively. The positive rate of gene qacE△1-sul1 was 50.0%. CONCLUSIONS The resistance of P. aeruginosa isolated from burned patients is a serious issue.There is high positive percentage of qacE△1-sul1 gene in P. aeruginosa isolated from burned patients.

OBJECTIVE To analyze the cause of Acinetobacter baumannii resistance to ?-lactam and the aminoglycoside-modifying antibacterials. METHODS Three-dimensional test was used to analyze and classify the ?-lactamases. Proper primers was used to do PCR and determined by sequencing. RESULTS A. baumannii clinical isolate harbored blaOXA2-23,blaTEM and blaADC genes and aac(3)-Ⅰ,aac(6')-Ⅰb and ant(3″)-Ⅰ aminoglycoside-modifying enzyme genes. CONCLUSIONS An A. baumannii strain which carries TEM,OXA-23,ADC ?-lactams and aac(3)-Ⅰ,aac(6')-Ⅰb,ant(3″)-Ⅰ aminoglycoside-modifying enzyme genes is detected.

OBJECTIVE To investigate the 16S rRNA methylase gene and aminoglycoside-modifying enzyme genes in Pseudomonas aeruginosa isolated from burned patients. METHODS GNS-448 and K-B tests were performed to detect the susceptibility to 19 kinds of antimicrobial agents against these strains. 16S rRNA methylase gene and aminoglycoside-modifying enzyme genes were amplified by polymerase chain reaction (PCR) and verified by DNA sequencing. RESULTS The 32 isolated strains were all resistant to ampicillin,cefuroxime,cefoxitin,SMZ-TMP,The sensitive rates to amikacin and gentamicin were 68% and 46.9%,respectively. The resistant rates to imipenem and meropenem were 68.8% and 59.4%,respectively. The 16S rRNA methylase gene and aminoglycoside-modifying enzyme genes including aac(6')-Ⅰb,aac(6')-Ⅱ,ant(3″)-Ⅰ,ant(2″)-Ⅰ and rmtB were found and positive rates were 9.4%,3.1%,28.1%,25.0% and 3.1%,respectively. A novel subtype of aac(6')-Ⅰb was reported firstly. CONCLUSIONS There are high positive percentage of 16S rRNA methylase gene and aminoglycoside-modifying enzyme genes in P. aeruginosa isolated from burned patients. P. aeruginosa resistance to aminoglycoside relates to the existence of 16S rRNA methylase gene and aminoglycoside-modifying enzyme genes.