1.Changes of methemoglobin percentage in patients with anemia due to the bone-marrow depression
Journal of Practical Medicine 2000;383(6):91-92
30 patients with anemia due to bone marrow depression and 60 healthy people (control) ages of 17-50 were monitored the rate of MetHb, number of erythrocyte and amount of Hb. The results have showns that percentage of MetHb in the control was the same as other studies. The average number of erythrocyte in patients with anemia was 1,85 +/- 0,5 million/lit, significant lower than this of control. The percentage of MetHb in patients with anemia due to the bone marrow depression much higher than normal level. The disease occurred mainly in young men and its progress also was more servere.
Anemia
;
Bone marrow
;
Methemoglobin
2.The Effect of Bupivacaine on the Methemoglobin Formation .
Korean Journal of Anesthesiology 1982;15(1):74-80
The changes of methemoglobin concentration in the blood after the use of lidocatine or Marcaine were investigated in the human and the rabbits. In the human the drugs were give by the epidural route and intramuscularly in rabbits. Lidocaine did not increase the methemoglobin concentration in humans but marcaine increased the methemoglobin concentration in the blood.
Bupivacaine*
;
Humans
;
Lidocaine
;
Methemoglobin*
;
Rabbits
3.Methemoglobin and treatment of hypermethemolobinemia
Journal of Medical Research 2002;18(2):61-65
The hyper-methemoglobinemia causes the oxygen transfer disorder. The clinical symptoms depends on the level of methemoylobinemia. Hypermethemoglobinemia can be congenital or acquired hypermethemoglobinemia. The congenital hypermethemoglobinemia caused by partial or total methemoglobine reeducates deficiency in patients with rare genetic heterozygous enzyme deficiency due to the abnormal hemoglobin. The acquired hypermethemoglobinemia occurred after exposure to colour compound, food and drinking water with nitrate or drugs. The determination of causative agent of hypermethemoglobin help treating more effectively
Methemoglobin
;
Hemoglobins, Abnormal
;
Therapeutics
;
Methemoglobinemia
4.Effect of N-acetylcysteine on Dapsone-Induced Methemoglobinemia.
Wen Joen CHANG ; Seung Ho KIM ; Hahn Shick LEE
Journal of the Korean Society of Emergency Medicine 1997;8(2):150-154
BACKGROUND: To determine whether N-acetylcysteine(NAC) reduces methemoglobin. METHOD: We carried out an in vivo experiment in rats, weighed about 300g. Each rat was ingested 200mg of dapsone(4,4'diaminodiphenyl sulphone) to induce methemo- globinemia. After 1 hour, 30 rats were received NAC 160mg(2ml) and another 30 rats, served as control, were received 2ml of normal saline orally,4 times hourly. Serum methemoglobin concentrations were checked 1,6, and 24 hours after dapsone ingestion. RESULT: The methemoglobin concentrations on each time were 27.1+/-5.8%,23.5+/-8.8%, 17.1+/-6.4%(mean+/-SD) in control group and 25.5+/-6.3%, 25.5+/-8.8%, 65.5+/-31.2%(mean+/-SD) in NAC group. There were no differences on methemoglobin concentrations at 1 and 6 hours between two groups(P>.05). At 24 hours, the methemoglobin concentrations of NAC group was significantly higher than those of control group(P<.01). CONCLUSION: NAC had no therapeutic effects on dapsone induced methemoglobinemia in this experimental setting.
Acetylcysteine*
;
Animals
;
Dapsone
;
Eating
;
Methemoglobin
;
Methemoglobinemia*
;
Rats
5.Evaluation of the Blood Gas Analyzer GEM Premier 4000.
Hee Young CHUNG ; Hee Jung CHUNG ; Sail CHUN ; Woochang LEE ; Won Ki MIN
Journal of Laboratory Medicine and Quality Assurance 2009;31(1):207-214
BACKGROUND: We evaluated the performance of the GEM Premier 4000 (Instrumentation Laboratory, USA), a new blood gas/electrolytes/co-oximetry analyzer, according to the Clinical and Laboratory Standard Institute (CLSI) guidelines. METHODS: Within-run precision, total-run precision, linearity and sample-related carryover were analyzed using quality control materials at three different concentration levels for each analytes. Correlation was compared with the routinely used NOVA CCX2 (Nova Biomedical, USA) with patients' whole blood samples. RESULTS: The within-run and the total-run precisions of the GEM Premier 4000 showed very low CV of 0.04~4.40% and 0.06~4.11%, respectively, in all parameters except the lactate, which had CV of 5.58% in Level 1 QC material. The system showed a good linearity (r2=0.997~1.000, systemic error=0.00~0.20%) for all items. Sample-related carryover was -4.35%~0.15%. In comparison with the NOVA CCX2 instrument, correlation was high in all parameters with the r value ranging from 0.983-0.999 except for carboxyhemoglobin (r=0.804) and methemoglobin (r=0.010) whose concentrations were in the lower level. CONCLUSIONS: GEM Premier 4000 showed good analytical performance required for blood gas analyzer in its precision, linearity, sample-related carryover, and close correlation with NOVA CCX2. It fulfills most of the requirements for both point-of-care and laboratory use.
Carboxyhemoglobin
;
Lactic Acid
;
Methemoglobin
;
Quality Control
6.A Patient with "Rebound Methemoglobinemia" during Treatment of Methemoglobinemia Caused by Propanil Intoxication.
Kyeong Hoon SEON ; Yong Jin PARK ; Soo Hyung CHO
Journal of the Korean Society of Emergency Medicine 2013;24(6):775-779
Methemoglobinemia results from the oxidation of ferrous iron (Fe++) to ferric iron (Fe+++) within hemoglobin. It is caused by various etiologies, including the herbicide Propanil. Patients with low levels of methemoglobin (metHb) are asymptomatic but symptomatic patients and patients with high levels of metHb require treatment. Methylene blue is the first choice for the treatment of methemoblobinemia, but has some complications such as hemolytic anemia and rebound methemoglobinemia. We report the case of a 91-year-old woman who died of aniline herbicides poisoning. The level of metHb in her blood was initially 20.7% and her mental status was drowsy. She was intravenously treated with methylene blue within a therapeutic range for methemoglobinemia. After treatment with methylene blue, the level of metHb decreased but later increased above 20%. Methylene blue treatment was repeatedly attempted, but the patient suffered from hemolytic anemia and rebound methemoglobinemia. The patient finally died from renal failure and cardiopulmonary collapse. We must be careful because methemoglobinemia can occur even when treated pesticides such as propranil.
Anemia, Hemolytic
;
Female
;
Herbicides
;
Humans
;
Iron
;
Methemoglobin
;
Methemoglobinemia*
;
Methylene Blue
;
Pesticides
;
Poisoning
;
Propanil*
;
Renal Insufficiency
7.Effects of freeze-drying process on polymerized human placenta hemoglobin.
Lanzhen ZHAO ; Shen LI ; Fengjuan LI ; Jinfeng WANG ; Chengmin YANG
Journal of Biomedical Engineering 2013;30(5):1052-1057
The present study was aimed to investigate the influence of freeze-drying on the quality of polymerized human placenta hemoglobin (PolyPHb). The PolyPHb solution was freeze-drying under suitable conditions. Hemoglobin concentration, methemoglobin (MetHb) content, UV spectrum, Fe3 content, oxygen-carrying capacity, pH, the average molecular weight and its distribution, circular dichroism, oxygen equilibrium curve and other indicators were measured before and after freeze-drying. The appearance, residual water content, rehydration time of the lyophilized product were also evaluated. The results showed that there was no significant difference on all the indicators measured above, which indicated that freeze-drying process had no effect on the physical and chemical properties of PolyPHb, as well as on its biological activity. Therefore, the properties of PolyPHb were stable during this freeze-drying process and could be preserved after such freeze-drying process.
Blood Substitutes
;
Female
;
Freeze Drying
;
methods
;
Hemoglobins
;
chemistry
;
Humans
;
Methemoglobin
;
analysis
;
Placenta
;
chemistry
;
Polymerization
;
Pregnancy
8.Comparison between hemolysis percentage measurement and hemiglobincyanide measurement for standardizing the evaluation of hemolytic properties of biomaterials.
Lingli ZHANG ; Weijing ZHU ; Yanfei TAN ; Shuxin QU ; Xingdong ZHANG
Journal of Biomedical Engineering 2004;21(1):111-137
In this study, two methods--the supernatant hemoglobin spectrophotometry, i.e. hemolysis percentage measurement(according to ISO/TR 7405) and the hemiglobincyanide measurement(according to ISO 10993-4)--were used to assay the hemolytic properties of hydroxyapatite(bioceramics) and collagen (polymer). The results showed that the conclusions drawn from using the two methods were basically consistent, and the latter was more sensitive, stable and comparable. However, some of the procedures in the hemiglobincyanide method were not defined in details. So based on our experiments we have offered some suggestions and improvements, which do not deviate from ISO and ASTM standards, for hiher practicability of usig it in standardizing the evaluation of the hemolytic properties of biomaterials. Hemiglobincyanide measurement is worthy of wider application.
Animals
;
Collagen
;
Durapatite
;
Hemoglobins
;
Hemolysis
;
Humans
;
Materials Testing
;
methods
;
Methemoglobin
;
analogs & derivatives
;
Rabbits
9.Fatal Propanil Pesticide Poisoning Presenting with Methemoglobinemia.
Seon Hee WOO ; Byung Hak SO ; Kyoung Ho CHOI ; Kyu Nam PARK ; Won Jae LEE
Journal of the Korean Society of Emergency Medicine 2006;17(3):268-272
Acute propanil pesticide poisoning is rare and propanil is known to have a low toxicity. Propanil metabolite induces the conversion of Fe2+ in hemoglobin to Fe3+, forming methemoglobin. Patients with low metHb levels are asymptomatic, but severe methemoglobinemia produces headache, dizziness, decreased consciousness, convulsion, and death. Thus, delayed diagnosis in patients with severe methemoglobinemia can be lethal. We describe the case of a 60-year-old man who died of acute propanil pesticide poisoning. He presented with severe methemoglobinemia. Early methylene blue therapy is recommended for propanil pesticide poisoning patients with symptoms and signs of methemoglobinemia.
Consciousness
;
Delayed Diagnosis
;
Dizziness
;
Headache
;
Humans
;
Methemoglobin
;
Methemoglobinemia*
;
Methylene Blue
;
Middle Aged
;
Poisoning*
;
Propanil*
;
Seizures
10.Analysis of Contaminated Ground Water Inducing Methemoglobinemia and Epidemiologic Investigation of Contaminated Ground Water.
Bu Heon LEE ; Ji Young SUH ; Nam Su KIM ; Hang LEE ; Young Yeul KIM ; Bo Youl CHOI ; Hung Bae PARK ; Min Young KIM ; In Hak YEO
Journal of the Korean Pediatric Society 1995;38(4):507-512
PURPOSE: Drining of Nitrate-contaminated water has been the most common cause of acquired methemoglobinemia. We had cyanotic infant diagnosed as methemoglobinemia caused by feeding with powdered formula mixed with nitrate-contaminated ground water. METHODS: We had done epidemiologic investigation to identify the cause of an infant's methemoglobinemia. Analysis of ground water and blood test of involved family members and neighbors were performed RESULTS: Analysis of the 8 household ground water revealed high level of nitrate(29837 ppm), indicating contamination of water as the cause of methemoglobinemia. We had done blood tests to evaluate the methemoglobin level of 65 people in the neighborhood ; among them, there was no infant and we could not find another cyanotic case of methemoglobinemia. They had normal blood level of methemoglobinemic concentration. CONCLUSIONS: If ground water will be used for drinking water, especially for formula water, epidemiologic investigation and analysis of ground waters should be recommended.
Drinking Water
;
Family Characteristics
;
Groundwater*
;
Hematologic Tests
;
Humans
;
Infant
;
Methemoglobin
;
Methemoglobinemia*
;
Residence Characteristics