1.Encapsulation of Metarhizium anisopliae spores with zeolite nanoparticles and magnesium silicate nanoparticles against mortality and lethal times of Crocidolomia pavonana larvae
Nawir Ilhami ; Wawan Hermawan ; Mia Miranti ; Melanie ; Hikmat Kasmara ; I Made Joni ; Camellia Panatarani
Malaysian Journal of Microbiology 2020;16(5):407-413
Aims:
Cruciferae crops failure such as cabbage generally due to heavy attacks by of Crocidolomia pavonana larvae.
Metarhizium anisopliae is a species of fungus that can infect more than 200 insect pests including C. pavonana larvae.
However, the direct application of M. anisopliae spore in the field is susceptible to UV rays from the sun, which can
decrease the efficiency of M. anisopliae. Thus, this study aims to investigate the encapsulation performance of M.
anisopliae spore with zeolite and magnesium silicate nanoparticles in terms of mortality and lethal time against 3
rd instar
C. pavonana larvae.
Methodology and results:
Zeolite and magnesium silicate nanoparticles were chosen because they are non-toxic,
environmentally friendly and able to maintain moisture, so that they are best used as protective materials for spores. The
experiment was designed by Randomized Block Design (RBD) with a single factor. The obtained results showed that
encapsulation of M. anisopliae spores with zeolite nanoparticles coating agent increased the mortality rate to 92.5% and
accelerated the lethal time up to 1.075 days compared to only spore correspondingly 27.5% and 2.235 days. The M.
anisopliae spores encapsulated with magnesium silicate nanoparticles also increased the mortality to 85.0% and
accelerated the larval lethal time up to 1.150 days.
Conclusion, significance and impact of study
Metarhizium anisopliae spores that encapsulated with zeolite
nanoparticles coating received higher mortality and faster lethal time to C. pavonana compared to those encapsulated
with magnesium silicate nanoparticles. The encapsulation formulation of these two coatings can be used as
bioinsecticide in controlling C. pavonana larvae.
Metarhizium
2.Effects of nutrient additives and incubation period on sporulation and viability of the entomopathogenic fungus, Metarhizium anisopliae (Hypocreales: Clavicipitaceae)
Najihah Abdul Halim ; Johari Jalinas ; Azlina Zakaria ; Samsudin Amit ; Zazali Chik ; Idris Abdul Ghani ; Wahizatul Afzan Azmi
Malaysian Journal of Microbiology 2021;17(1):97-102
Aims:
Metarhizium anisopliae is an entomopathogenic fungus (EPF) that exists naturally in the environment and
potentially be used as a biological control agent against many insect pests. This study aims to evaluate the effect of
nutrient additives on the yield and viability of M. anisopliae spore and to determine the optimum incubation period for
maximum spore production.
Methodology and results:
In this study, M. anisopliae was cultivated by solid-state fermentation using rice as a growth
medium. Three different nutrient additives were examined which aimed to maximize the production of M. anisopliae
spores. Among the three nutrient additives evaluated, yeast (1.84 ± 0.04 g) supported better growth and spore
production than molasses (0.58 ± 0.04 g) and palm oil (0.47 ± 0.09 g). The incubation period between 2-6 weeks
produced higher spore yield (0.97 ± 0.02 g spores) at week 4 with a better spore viability (86.30 ± 0.45%) at week 2.
Conclusion, significance and impact of study
Hence, it is suggested that the optimum incubation period is between
2 and 6 weeks after inoculation, and M. anisopliae could be mass produced in large quantities on rice substrate with the
addition of yeast as the nutrient additives.
Biological Control Agents
;
Microbial Viability
;
Metarhizium
3.Agrobacterium-Mediated Co-transformation of Multiple Genes in Metarhizium robertsii.
Israel Enrique PADILLA-GUERRERO ; Michael J BIDOCHKA
Mycobiology 2017;45(2):84-89
Fungi of the Metarhizium genus are a very versatile model for understanding pathogenicity in insects and their symbiotic relationship with plants. To establish a co-transformation system for the transformation of multiple M. robertsii genes using Agrobacterium tumefaciens, we evaluated whether the antibiotic nourseothricin has the same marker selection efficiency as phosphinothricin using separate vectors. Subsequently, in the two vectors containing the nourseothricin and phosphinothricin resistance cassettes were inserted eGFP and mCherry expression cassettes, respectively. These new vectors were then introduced independently into A. tumefaciens and used to transform M. robertsii either in independent events or in one single co-transformation event using an equimolar mixture of A. tumefaciens cultures. The number of transformants obtained by co-transformation was similar to that obtained by the individual transformation events. This method provides an additional strategy for the simultaneous insertion of multiple genes into M. robertsii.
Agrobacterium
;
Agrobacterium tumefaciens
;
Fungi
;
Insects
;
Metarhizium*
;
Methods
;
Streptothricins
;
Virulence
4.Confirmation of Two Undescribed Fungal Species from Dokdo of Korea Based on Current Classification System Using Multi Loci.
Hye Won LEE ; Thi Thuong Thuong NGUYEN ; Hye Yeon MUN ; Haengsub LEE ; Changmu KIM ; Hyang Burm LEE
Mycobiology 2015;43(4):392-401
Using dilution plating method, 47 fungal isolates were obtained from a soil sample collected from Dokdo in the East Sea of Korea in 2013. In this study, two fungal isolates, EML-MFS30-1 and EML-DDSF4, were confirmed as undescribed species, Metarhizium guizhouense and Mortierella oligospora in Korea based on current classification system using multi loci including rDNA internal transcribed spacer, large subunit, small subunit, and beta-tubulin (BTUB) genes. Herein, detailed morphological descriptions on characters of the undescribed fungal species as well as their molecular phylogenetic status are provided with comparisons to related species.
Classification*
;
DNA, Ribosomal
;
Korea*
;
Metarhizium
;
Mortierella
;
Soil
;
Tubulin
5.Isolation and identification of Metarhizium from Citrus grandis 'tomentosa' GAP base.
Weisi MA ; Jiang XU ; Haili QIAO ; Jun CHEN ; Xiangming LI ; Rongmin QIN ; Huizhen CHENG
China Journal of Chinese Materia Medica 2012;37(7):887-891
A type of entomopathogenic fungus of soil in Citrus grandis 'tomentosa' production base was isolated and identified with morphological and molecular biological methods, including pathogenesis, spore characteristic and ITS sequence analysis were conducted. The results showed that eighteen entomopathogenic fungi strains were isolated from the Tenebrio molitor infected in the soil samples, which were identified as Metarhizium anisopliae var. anisopliae. Based on results above, we concluded that there was quantity of Metarhizium resources in this area. These provided the useful information for controlling some pests of C. grandis by using these strains of fungus.
Animals
;
Citrus
;
parasitology
;
Metarhizium
;
isolation & purification
;
physiology
;
Pest Control, Biological
;
methods
;
Soil Microbiology
6.Evaluation of Strains of Metarhizium anisopliae and Beauveria bassiana against Spodoptera litura on the Basis of Their Virulence, Germination Rate, Conidia Production, Radial Growth and Enzyme Activity.
Wanida PETLAMUL ; Poonsuk PRASERTSAN
Mycobiology 2012;40(2):111-116
Ten strains of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana were evaluated to find the most effective strain for optimization studies. The first criterion tested for strain selection was the mortality (> 50%) of Spodoptera litura larvae after inoculation of the fungus for 4 days. Results on several bioassays revealed that B. bassiana BNBCRC showed the most virulence on mortality S. litura larvae (80% mortality). B. bassiana BNBCRC also showed the highest germination rate (72.22%). However, its conidia yield (7.2 x 10(8) conidia/mL) was lower than those of B. bassiana B 14841 (8.3 x 10(8) conidia/mL) and M. anisopliae M6 (8.2 x 10(8) conidia/mL). The highest accumulative radial growth was obtained from the strain B14841 (37.10 mm/day) while the strain BNBCRC showed moderate radial growth (24.40 mm/day). M. anisopliae M6 possessed the highest protease activity (145.00 mU/mL) while M. anisopliae M8 possessed the highest chitinase activity (20.00 mU/mL) during 96~144 hr cultivation. Amongst these criteria, selection based on virulence and germination rate lead to the selection of B. bassiana BNBCRC. B. bassiana B14841 would be selected if based on growth rate while M. anisopliae M6 and M8 possessed the highest enzyme activities.
Beauveria
;
Biological Assay
;
Chitinase
;
Fungi
;
Germination
;
Larva
;
Metarhizium
;
Patient Selection
;
Spodoptera
;
Spores, Fungal
;
Sprains and Strains
7.Phylogenetic Analysis of Caterpillar Fungi by Comparing ITS 1-5.8S-ITS 2 Ribosomal DNA Sequences.
Joung Eon PARK ; Gi Young KIM ; Hyung Sik PARK ; Byung Hyouk NAM ; Won Gun AN ; Jae Ho CHA ; Tae Ho LEE ; Jae Dong LEE
Mycobiology 2001;29(3):121-131
This study was carried out to identify the phylogenetic relationships among several caterpillar fungi by comparing the sequences of internal transcribed spacer regions (ITS1 and ITS2) and 5.8S ribosomal DNA (rDNA) repeat unit. The sequences of ITS1, ITS2, and the 5.8S rDNA from 10 strains of Cordyceps species, 12 strains of Paecilomyces, 3 strains of Beauveria, 2 strains of Metarhizium and 1 strains of Hirsutella were amplified, determined and compared with the previously known Cordyceps species. The sequences of 5.8S rDNA were more conserved in length and variation than those of ITS regions. Although the variable ITS sequences were often ambiguously aligned, the conserved sites could be found. In the phylogenetic tree, the species generally divided into three clusters, supported by their morphology and/or host ranges. The 5.8S rDNA and ITS1 sequences among 10 species of Cordyceps militaris were identical and only one base pair in ITS2 sequence was different. Cordyceps sinensis and Cordyceps ophioglossoides were also clearly different, although they belonged to the same cluster. The GenBank database search of species revealed sister taxa of an entomogenous fungus. Metarhizium was used as an outgroup in all taxa.
Base Pairing
;
Beauveria
;
Cordyceps
;
Databases, Nucleic Acid
;
DNA, Ribosomal*
;
Fungi*
;
Host Specificity
;
Humans
;
Metarhizium
;
Paecilomyces
;
Phylogeny
;
Siblings
8.Entomopathogenic Fungi as Dual Control Agents against Both the Pest Myzus persicae and Phytopathogen Botrytis cinerea.
Hwi Geon YUN ; Dong Jun KIM ; Won Seok GWAK ; Tae Young SHIN ; Soo Dong WOO
Mycobiology 2017;45(3):192-198
The green peach aphid (Myzus persicae), a plant pest, and gray mold disease, caused by Botrytis cinerea, affect vegetables and fruit crops all over the world. To control this aphid and mold, farmers typically rely on the use of chemical insecticides or fungicides. However, intensive use of these chemicals over many years has led to the development of resistance. To overcome this problem, there is a need to develop alternative control methods to suppress populations of this plant pest and pathogen. Recently, potential roles have been demonstrated for entomopathogenic fungi in endophytism, phytopathogen antagonism, plant growth promotion, and rhizosphere colonization. Here, the antifungal activities of selected fungi with high virulence against green peach aphids were tested to explore their potential for the dual control of B. cinerea and M. persicae. Antifungal activities against B. cinerea were evaluated by dual culture assays using both aerial conidia and cultural filtrates of entomopathogenic fungi. Two fungal isolates, Beauveria bassiana SD15 and Metarhizium anisopliae SD3, were identified as having both virulence against aphids and antifungal activity. The virulence of these isolates against aphids was further tested using cultural filtrates, blastospores, and aerial conidia. The most virulence was observed in the simultaneous treatment with blastospores and cultural filtrate. These results suggest that the two fungal isolates selected in this study could be used effectively for the dual control of green peach aphids and gray mold for crop protection.
Aphids*
;
Beauveria
;
Botrytis*
;
Colon
;
Crop Protection
;
Farmers
;
Fruit
;
Fungi*
;
Insecticides
;
Metarhizium
;
Plants
;
Prunus persica
;
Rhizosphere
;
Spores, Fungal
;
Vegetables
;
Virulence
9.Virulence of Entomopathogenic Fungi Metarhizium anisopliae and Paecilomyces fumosoroseus for the Microbial Control of Spodoptera exigua.
Ji Hee HAN ; Byung Rae JIN ; Jeong Jun KIM ; Sang Yeob LEE
Mycobiology 2014;42(4):385-390
The beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae) is difficult to control using chemical insecticides because of the development of insecticide resistance. Several pest control agents are used to control the beet armyworm. Entomopathogenic fungi are one of the candidates for eco-friendly pest control instead of chemical control agents. In this study, among various entomopathogenic fungal strains isolated from soil two isolates were selected as high virulence pathogens against larva of beet armyworm. Control efficacy of fungal conidia was influenced by conidia concentration, temperature, and relative humidity (RH). The isolates Metarhizium anisopliae FT83 showed 100% cumulative mortality against second instar larvae of S. exigua 3 days after treatment at 1 x 10(7) conidia/mL and Paecilomyces fumosoroseus FG340 caused 100% mortality 6 days after treatment at 1 x 10(4) conidia/mL. Both M. anisopliae FT83 and P. fumosoroseus FG340 effectively controlled the moth at 20~30degrees C. M. anisopliae FT83 was significantly affected mortality by RH: mortality was 86.7% at 85% RH and 13.4% at 45% RH. P. fumosoroseus FG340 showed high mortality as 90% at 45% RH and 100% at 75% RH 6 days after conidia treatments. These results suggest that P. fumosoroseus FG340 and M. anisopliae FT83 have high potential to develop as a biocontrol agent against the beet armyworm.
Beta vulgaris
;
Fungi*
;
Humidity
;
Insecticide Resistance
;
Insecticides
;
Larva
;
Metarhizium*
;
Mortality
;
Moths
;
Paecilomyces*
;
Pest Control
;
Soil
;
Spodoptera*
;
Spores, Fungal
;
Virulence*
10.Roles of phosphatases in pathogen infection: a review.
Pei ZHU ; Xinqiang LI ; Zhenlun LI
Chinese Journal of Biotechnology 2012;28(2):154-163
Phosphatases play a key role not only in cell physiological functions of an organism, but also in host-pathogen interactions. Many studies demonstrated that some Gram-negative pathogenic bacteria could evade host immunity and promote pathogenicity by injecting phosphatases into host cells through type III secretion system. However, there were few reports about pathogenic fungi evading the immunity of hosts. Our researches indicated that the entomogenic fungus Metarhizium anisopliae could dephosphorylate the signal transduction substance of locust humoral immunity specifically in vitro by secreting extracellular protein tyrosine phosphatase, which implied that the fungus might interfere with the immune defense of locust. To provide reference for further studies of the functions of phosphatases, we reviewed the types of phosphatases and their roles in pathogen infection.
Animals
;
Fungal Proteins
;
metabolism
;
Fungi
;
enzymology
;
physiology
;
Gram-Negative Bacteria
;
enzymology
;
physiology
;
Grasshoppers
;
immunology
;
microbiology
;
Host-Pathogen Interactions
;
Metarhizium
;
enzymology
;
Phosphoric Monoester Hydrolases
;
classification
;
physiology
;
secretion