1.Expression and significance of thrombospondin-1 in myocardium of type 2 diabetic cardiomyopathy rats
Ming ZHONG ; Wei ZHANG ; Ya MIAO ; Peili BU ; Hui SUN ; Xiao MA ; Huiping GONG ; Mengxiong TANG ; Yun ZHANG
Chinese Journal of Pathophysiology 1999;0(09):-
AIM:To investigate the expression and significance of thrombospondin-1(TSP-1)in left ventricular myocardium of type 2 diabetic cardiomyopathy(DCM).METHODS:The rat model of DCM was established by eating a high-fat diet together with injection of low dose streptozocin(30 mg/kg)intrapertoneally.After 12 weeks,the content of collagen was quantified by Masson staining.The mRNA level of TSP-1 was determined by quantification real-time RT-PCR,while the protein level of TSP-1 was analyzed by Western blotting and immunohistochemistry.RESULTS:Compared with the control group,the content of collagen in the DCM group was increased greatly(11.01?3.05 vs 16.92?3.18,P
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