Objective:Through the closed loop management mode,it realized the whole process of material information could be traced back,especially in the medical business level.Methods:Based on the requirement of management accounting,the integrated accounting and business information were in accordance with management accounting.Results and Conclusion:It set up different management nodes in the closed loop of the materials,such as medicine,sanitary materials and test reagents,so as to get the relevant information.The problems of the extensive management of materials could be solved to improve the efficiency of the management of hospital materials.

Through collecting the economic operation information of the society-run blood purification institutionrs,the cost is divided into fixed cost,variable cost and mixed cost according to the relationship between the cost and the number of dialysis.It formulated the standard cost model of blood purification institutions,according to the county and city classification,the basic situation of the size and capacity of the organization were assumed.It mainly included standard labor costs,standard drug costs,standard health materials fees and standard professional equipment depreciation and other information,which was benefitial for industry decision-making and internal fine management.

Starting from the reform result and influence of A County of public hospital reform,the reform experience and forming mechanism are analyzed to provide references for county level public hospital reform.Through taking controlling medical fees as the core,replacing policy break with mechanism break has a realistic meaning and applicable value.But the administrative type of passive controlling fees and squeezing the income of medical workers will produce a negative influence for the long-term development of health industry and for the future interest of local people.

Methods:To describe the connotation and construction mechanism of integrated medical service system.Methods:Based on the data of hierarchy diagnosis situation in China Statistical Yearbook from 2005 to 2015,the traditional medical service system and integrated medical service system were compared from the objective,method and core,while the construction mechanism of integrated medical service system was analyzed from the lead,control,supervision and adjustment.Results:Under the background of Health China,integrate medical system based on the purpose of health community,the method of division and cooperation and the core of cost management was different from the traditional medical service system which based on the aim of medical service,the method of competition and split and the core of ‘business of water'.Hierarchy diagnosis was the appearance/mehod of regional integrated diagnosis and the result from medial resource optimized allocation under the integrated medical service system.Constructing the integrated medical service system was the basic demand of medicine and health system reform.Conclusion:Constructing the integrated medical service system should be led by the performance appraisal of medical institutions,controlled by the cost accounting and adjusted by medical service prices,so that to make the benefits for the demander,the provider and the third party of medical service system to construct and share the health community.

Objective To observe the benefits and harms of nilestriol in elderly women with hypertension.Methods Forty-one elderly women with hypertension who had received antihypertensive therapy were randomly divided into two groups. Women in group A (n=21) received oral nilestriol, wherase those in group B(n=20) received oral placebo for six months. Blood sugar(Bs), blood lipids, soluble intercellular adhesion molecule 1(sICAM-1),E-selectin and plasma fibrinogen(Fbg),tissue-type plasminogen activator antigen(tPA),plasminogen activator inhibitor 1(PAI-1),von Willebrand factor(vWF) were measured before starting treatment and 6th month after treatment.Results Levels of total cholesterol(TC),Fbg,tPA,PAI-1,vWF,sICAM-1,E-selectin in group A were significantly lower than those in group B.Conclusions In elderly women with hypertension, nilestriol could improve the metabolism of lipids,endotheliocytes function,inflammation factors expression,thrombosis and hemostasis.

Background and Aim:Sulfotransferase(SULT)-mediated sulfation and steroid sulfatase(STS)-mediated desulfation represent two critical mechanisms that regulate the chemical and functional homeostasis of endogenous and exogenous molecules.STS catalyzes the hydrolysis of steroid sulfates to form hydrox-ysteroids.Oxygenated cholesterol derivative oxysterols are known to be endogenous ligands of the liver X receptor(LXR),a nuclear receptor with anti-cholestasis activity,whereas the sulfated oxysterols antagonize LXR signaling.The conversion of sulfated oxysterols to their non-sulfated counterparts is catalyzed by STS.The aim of this study is to determine whether STS can alleviate cholestasis by increasing the activity of LXR. Methods:Liver-specific STS transgenic mice were created and subject to the lithocholic acid(LCA)-induced model of cholestasis. Results:Transgenic overexpression of STS in the liver promoted bile acid elimination and alleviated LCA-induced cholestasis.The protective effect of the STS transgene was associated with the activation of LXR and induction of LXR target genes,likely because of the increased conversion of the antagonistic oxy-sterol sulfates to the agonistic oxysterols. Conclusions:STS has a novel function in controlling the homeostasis of bile acids by regulating endog-enous LXR ligands.

Objective:To investigate the action targets and mechanism of celastrol in alleviating liver inflammatory injuries using network pharmacology; To verify the key targets through liver ischemia-reperfusion injury inducing inflammation mouse model.Methods:The targets of celastrol were integrated by searching SymMap, BATMAN-TCM, TCMSP, HIT 2.0, LigTMap, SEA, SwissTarget, Super-PRED, STITCH databases. The molecular targets of hepatic inflammatory injuries were investigated by GeneCards and DisGeNET databases. The intersection of drug targets and disease targets was obtained by Venn diagram to obtain the possible targets of celastrol in alleviating hepatic inflammatory diseases. Cytoscape 3.9.1 software was used to analyze the key targets of protein interaction (PPI) networks based on STRING database, and enrichment analysis was conducted through DAVID database. Based on the key targets, the ceRNA network was mapped by retrieving the starBase database. Molecular docking was used to evaluated the binding of celastrol with the key target proteins. The mice were divided into sham-operation solvent group, sham- operation medication group, model group, and Tripterygium wilfordii extract low- (0.1 mg/kg), medium- (0.3 mg/kg), and high- (1 mg/kg) dosage groups according to body weight, with 3-4 mice in each group. After 7 days of corresponding drug intervention, except for the sham-operation solvent group and sham-operation medication group, other groups were prepared with ischemia-reperfusion induced liver inflammation mouse models. The serum transaminase levels in mice were detected; the pathological morphology of mouse liver tissue was observed using HE staining; the expressions of IL-6 and TNF-α were detected in liver tissue using immunohistochemistry staining.Results:The key targets of celastrol in alleviating liver inflammation were inflammatory cytokines such as IL6 and TNF. The analysis of functional enrichment results showed that the key signaling pathways of Tripterygium wilfordii extract in reducing liver inflammatory injury included inflammatory response, cell apoptosis and proliferation, HIF1, and other pathways. Triptolide pretreatment could reduce serum aminotransferase level ( P<0.01) and liver inflammatory factors expression such as IL-6 and TNF-α ( P<0.05, P<0.01) after hepatic ischemia-reperfusion. Conclusion:Celastrol can alleviate hepatic ischemia-reperfusion injury, and its mechanism is closely related to the reduction of inflammatory factors such as IL-6 and TNF-α and the alleviation of hepatic inflammatory injury.

Hospitals without disciplines have no performance performance,the focus of performance reform lies in mechanism transformation.Hospital performance management and discipline construction are interdependent relationships.Performance management provides guidance and motivation for the process of discipline construction,the construction of disciplines provides a value basis for the business connotation of performance management.Guiding discipline construction by clarifying the disease efficacy system.Clarify discipline construction by refining the technical system.Quantify discipline construction by organizing departmental projects.Transform financial and personnel performance assessment into technical value management.The conjugate mechanism can trigger and drive the deployment,support,and fulfillment of business.Performance management plans are only effective when they are publicly available.Only solutions that comply with business logic can be made public.By mining valuable data,we tap into potential business value.

Objective:To explore eye movement characteristics in newly diagnosed, drug-naive Parkinson′s disease (PD) patients and their correlation with motor and non-motor symptoms.Methods:Seventy-five newly diagnosed, drug-naive PD patients and 46 healthy controls (HCs) were included in this cross-sectional study. Patients were recruited from the Department of Neurology, Shanghai Ninth People′s Hospital, Shanghai Jiao Tong University School of Medicine from November 2017 to December 2021, while HCs were recruited from the local community during the same period. For PD patients, motor severity was measured with the modified Hoehn and Yahr stage, Movement Disorder Society Unified Parkinson′s Disease Rating Scale part Ⅲ and the Freezing of Gait questionnaire. Non-motor symptoms were evaluated by serial scales such as Non-Motor Symptoms Questionnaire, 16-item odor identification test from Sniffin Sticks, 17-item Hamilton Rating Scale for Depression, Chinese version of Mini-Mental State Examination, Montreal Cognitive Assessment Basic and REM Behavior Disorder Screening Questionnaire. All subjects underwent oculomotor test including pro-saccade task and smooth pursuit eye movement (SPEM) task in the horizontal direction via videonystagmography. Visually guided saccade latency, saccadic accuracy and gain in SPEM at three frequencies (0.1, 0.2, 0.4 Hz) of the horizontal axis were compared between the 2 groups. The association between key oculomotor parameters and clinical phenotypes was explored in PD patients. The receiver operating characteristic (ROC) analyses of eye movement parameters as independent factors were also performed for detecting PD from HCs, then combining the saccadic latency, saccadic accuracy and the most significant SPEM gain (0.4 Hz) as the model to distinguish PD from HCs.Results:Relative to HCs, newly diagnosed, drug-naive PD patients showed prolonged saccadic latency [(210.4±41.3) ms vs (191.3±18.9) ms, t=-3.445, P=0.001] and decreased saccadic accuracy (88.4%±6.8% vs 92.2%±6.1%, t=3.064, P=0.003). SPEM gain in PD was uniformly reduced at each frequency(0.1 Hz: 0.68±0.15 vs 0.74±0.14, t=2.261, P=0.026; 0.2 Hz: 0.72±0.16 vs 0.79±0.16, t=2.704, P=0.008; 0.4 Hz: 0.67±0.19 vs 0.78±0.19, t=2.937, P=0.004). The ROC analyses of saccade latency, saccadic accuracy and gain in SPEM at 0.1, 0.2, 0.4 Hz as independent factors for detecting PD from HCs showed that the area under the curve (AUC) of each parameter was lower than 0.7: the AUC of saccade latency was 0.641 ( P=0.010), the AUC of saccadic accuracy was 0.681 ( P=0.001), the AUC of gain in SPEM at 0.1 Hz was 0.616 ( P=0.032), at 0.2 Hz was 0.652 ( P=0.005), at 0.4 Hz was 0.660 ( P=0.003). Combining the saccadic latency, saccadic accuracy and the most significant SPEM gain (0.4 Hz) revealed that the model could significantly distinguish PD from HCs with an 80.4% sensitivity and a 73.3% specificity (AUC=0.780, P<0.001). Prolonged saccadic latency was correlated with long disease duration ( β=0.334, 95% CI 0.014-0.654, P=0.041), whereas decreased SPEM gain was associated with severe motor symptoms in newly diagnosed drug-naive PD patients (0.1 Hz: β=-0.004, 95% CI -0.008--0.001, P=0.036; 0.4 Hz: β=-0.006, 95% CI -0.011--0.001, P=0.012). Conclusions:Ocular movements are impaired in newly diagnosed, drug-naive PD patients. These changes could be indicators for disease progression in PD.

Numerous studies on cancers, biopharmaceuticals, and clinical trials have necessitated comprehensive and precise analysis of protein O-glycosylation. However, the lack of updated and convenient databases deters the storage of and reference to emerging O-glycoprotein data. To resolve this issue, an O-glycoprotein repository named OGP was established in this work. It was constructed with a collection of O-glycoprotein data from different sources. OGP contains 9354 O-glycosylation sites and 11,633 site-specific O-glycans mapping to 2133 O-glycoproteins, and it is the largest O-glycoprotein repository thus far. Based on the recorded O-glycosylation sites, an O-glycosylation site prediction tool was developed. Moreover, an OGP-based website is already available (http://www.oglyp.org/). The website comprises four specially designed and user-friendly modules:statistical analysis, database search, site prediction, and data submission. The first version of OGP repository and the website allow users to obtain various O-glycoprotein-related information, such as protein accession Nos., O-glycosylation sites, O-glycopeptide sequences, site-specific O-glycan structures, experimental methods, and potential O-glycosylation sites. O-glycosylation data mining can be performed efficiently on this website, which will greatly facilitate related studies. In addition, the database is accessible from OGP website (http://www.oglyp.org/download.php).