Objective To investigate the effect of recombinant human erythropoietin (rhEPO) on the expression of glutamine synthetase (GS) of cultured rat retinal Müller cells in high glucose environment in vitro.Methods Mtüller cells were isolated from retinas of 10 Sprague-Dawley rats at postnatal day three to five by trypsin digestion,and were randomly divided into six groups,including normal control group,high glucose group,high glucose +5 U/ml rhEPO group,high glucose+ 10 U/ml rhEPO group,high glucose+ 20 U/ml rhEPO group,high glucose+40 U/ml rhEPO groups.After 48 hours,the apoptosis of retinal Müller cells were assayed by terminal transferase-mediated DNA end labelling assay,and the expression levels of GS protein were detected with semi-quantitative immunocytochemistry.Results Compared with the normal control group,the cell viability and GS protein were reduced while the cell death increased in Müller cells cultured in high glucose,the difference was statistically significant (t =27.4,P ＜ 0.01).Compared with the high glucose group,rhEPO treatment reduced the apoptotic Müller cells (t=857.2,2 374.6,2 473.2,2 537.7; P＜0.01),induced the expression of GS proteins (t=3.2,18.0,22.5,26.4; P＜0.05).Conclusions rhEPO can protect Müller cells from apoptosis under high glucose condition.The mechanism may be related to its function to up-regulate the GS protein expression,promote glutamic acid cycle,and reduce the excitotoxicity effects of high concentration of glutamate.

Intracellular calcium is one of the crucial signalings that modulate various physiological and pathological processes.Abnormal calcium signaling is closely associated with invasion and migration of neoplasms.Calcium regulates focal adhesion turnover,cytoskeletal reorganization and other processes about tumor cell movement through contacting with multiple downstream proteins,such as calpain,PYK2 and S100 protein family.Several calcium entry channels including store-operated calcium channels and transient receptor potential channels participate in the invasion and migration of tumor cells.Pathological calcium signaling may be a potential target in anti-invasion and anti-migration therapy.

This paper presents a development model of resource information institute from organic integration the institute of information and library.The all data of resources-information institute come from and serve for theoretical research,clinical practice,and scientific research of TCM.Comparing with usual research institute and library,the resource information institute has five dominant positions,which are subjects,scientific researches,resource,professionals and services.

EML4-ALK fusion oncogene represents a new molecular target which appears mainly in lung adenocarcinoma.EML4-ALK is detected more frequently in young non-small-cell lung cancer patients who never or light smoke.ALK inhibitors(crizotinib)for the treatment of the EML4-ALK-positive non-small cell lung cancer has a high response rate.This finding can improve the individual treatment of non-small cell lung cancer.

Informatics of traditional Chinese medicine is an emerging subject.This dissertation analyzes the connotation and scope of the study of traditional Chinese medicine informatics.Through investigating the demand for personnel talents of Chinese medicine hospital and Information Institute of TCM,we comment on with what theoretical basis and practical abilities the TCM information personnel talents should possess.It also proposes the training mode and optimization measures of TCM Informatics education.

Combined therapy consisting of radiotherapy,chemotherapy and palliative surgery is the primary management for locally advanced pancreatic cancer,with the purpose of prolonging survival and improving life quality.Gemcitabine is the standard chemotherapy at present.Gemcitabine-based combinations show a definite effect,and the combination with targeted drugs also has a certain degree of efficacy.The development of new chemotherapy drugs and targeted drugs provides more ways for the teatment of locally advanced pancreatic cancer.

As a new type of treatment for ovarian cancer,the fertility sparing surgery is put forward for patients who has fertility requirements and the surgical indication.But the indications and safety are still controversial at present on the international.The operation is based on the traditional surgery way,the retention of the uterus and at least one side of the ovary,and then the corresponding adjuvant chemotherapy and ovarian protection,and choosing the right pregnancy timing based on individual difference,to the excellent oncologic outcomes and the favorable reproductive outcomes.

The standard of traditional Chinese medicine (TCM) literature metadata is the technological basis and premise of the TCM resource administration and sharing. This paper studied the TCM literature metadata design condition by defining the object of description and discussing the necessity of TCM literature metadata preliminary. It put forward metadata framework structure and element extension principles so as to carry out the specific design work. In order to enhance the practicability of metadata, two means of expression including system table and metadata dictionary were designed with special provisions on description detail rules of metadata dictionary. The design principle of TCM literature metadata was summarized. The issue of balance between accuracy and entirety was considered in the metadata designing.

Objective To discuss the diagnostic values of transrectal ultrasound-guided puncture biopsy in prostate carcinoma(PCa).Methods A total of 238 patients with suspected PCa received tran-srectal ultrasound-guided puncture biopsy.Results Based on the results of biopsy pathology,88 cases (37.0%)were diagnosed as PCa,105 cases(44.1%)were benign prostate hyperplasia,15 cases (6.3%) were low-grade prostatic intraepithelial neoplasm,17 cases(7.1%)were atypical hyperplasia,8 cases(3. 4%)were adenomatoid tumor,and 5 cases(2.1%)were granulomatous prostatitis.Conclusion Tran-srectal ultrasound-guided puncture biopsy is accurate and safe.It is an effective method in diagnosing pros-tatic carcinoma.

Taking eugenics,family planning and reproductive human-assisted projects for instance,the article discusses the conflicts between modern biotechnology and the contemporary ethics.It distinguishes the concepts of life quality and that of health,and advocates that the modern medicine should conduct to be in pursuit of the high quality of life.Finally,it puts forward that we should upgrade the quality of individual's life in the circumstance of a stable and integrated communal ethics.Biotechnology may be developed immeasurably,but should be used restricted and morally.