BACKGROUND: The clinical application of neural stem cells (NSCs) in combination with gene therapy could be used to treat a variety of nervous system hereditary and acquired diseases. However, nervous system diseases are varying. Nerve tissue internal environment of distinctive diseases is also different. All these factors affect the therapeutic effect of NSCs. Moreover, interaction and regulation of a variety of exogenous genes in NSC gene therapy would greatly promote continued regeneration of nerve tissue.OBJECTIVE: To review application of NSCs in the nervous system diseases.METHODS: A computer-based online search of Medline database (2000-01/2009-08) and Tongfang database (2000-01/2009-08) was performed for related articles with key words "neural stem cells, gene, nervous system diseases". RESULTS AND CONCLUSION: A total of 88 English articles were collected. By reading the title and summary, 20 unrelated articles and 28 repetitive articles were excluded. Finally, 40 were reviewed. NSC as a new-type treatment has been used for somatic cell or transgenic vector, and has achieved a certain effect for cerebrovascular disease, brain damage disease, spinal cord injuries, neurodegenerative diseases, brain tumors, and genetic metabolic diseases. However, many key issues such as NSC proliferation, differentiation, migration and control mechanism have not been resolved. In addition, the microenvironment in nerve tissues with different diseases also influences NSC therapy.

Objective:To evaluate the effect of miR-497 on regulating cell proliferation and apoptosis by targeting Bcl-2 in liver cancer cells. Methods:We tested liver cancer tissue and para-carcinoma tissue and used RT-PCR or Western blot to detect the expression of miR-497and Bcl-2 protein. We also tested the liver cancer cel HepG2 transfected with miR-497 mimics and mimic control. The expressions of miR-497and Bcl-2 protein were detected by RT-PCR or Western blot. Cell proliferation activity was detected by the MTT method, cell apoptosis was detected by flow cytometry, and cel luciferase activity was detected by the dual-luciferase reporter gene experiment. Results:1) Compared with para-carcinoma tissue, the miR-497 expression of liver cancer tissue significantly decreased (P<0.05), whereas the Bcl-2 protein expression of liver cancer tissue significantly increased (P<0.05). 2) Compared with transfection mimic control, transfection miR-497 mimics could increase the miR-497 expression of liver cancer cel HepG2 (P<0.05) and decrease the Bcl-2 protein expression of liver cancer cel HepG2 (P<0.05). 3) Compared with transfection mimic control, co-transfection with miR-497 mimics and Bcl-2-WT could significantly decrease the luciferase activity of liver cancer cell HepG2 (P<0.05). 4) Compared with transfection mimic control, the proliferative activity of liver cancer cell HepG2 significantly decreased after transfection with miR-497 mimics (P<0.05). Compared with transfection miR-497 mimics, the proliferative activity of liver cancer cell HepG2 significantly increased after transfection with miR-497 mimics+Bcl-2 (P<0.05). 5) Compared with transfection mimic control, the total apoptosis rate of liver cancer cell HepG2 significantly increased after transfection with miR-497 mimics (P<0.05). Compared with transfection miR-497 mimics, the total apoptosis rate of liver cancer cell HepG2 significantly decreased after transfection with miR-497 mimics+Bcl-2 (P<0.05). Conclusion:In liver cancer cel s, miR-497 could target Bcl-2 to inhibit cel proliferation and enhance cell apoptosis.

Objective To explore the preventive and therapeutic effect of recombinant human interleukin-11(rhIL-11) on thrombocytopenia in children with non-lymphocytic leukemia after chemotherapy.Methods Sixteen children who had non-lymphocytic leukemia were divided into 2 groups by randomization,including a therapeutic group and a control group.RhIL-11[50 ?g/(kg?d)] was injected subcutaneously 24 h after chemiotherapy in the therapeutic group,and applied consecutively 10-14 days,and the control group was treated without RhIL-11.Duration of the thrombocytopenia,infusion of blood platelet,diversity of blood platelets counts and adverse effect were observed of the 2 groups.Differences between groups were examined using statistics analysis.Results There were 16 case-times(59.3%) in the therapeutic group that of could be cured without platelet transfusion,but that of control group only had 3 case-times(14.3%);the diffe-rence between 2 groups was significant(P

Objective: To investigate the relationship between metastasis-associated gene 1 (MTA1) expression and the biological behaviors on human breast cancer. Methods: SP immunohistochemical technique was used to detect the expression of MTA1 protein among 116 human breast cancer samples and matched normal breast tissues. Results: (1) The expression of MTA1 was significantly higher in the breast cancer tissues than those of matched normal breast tissues (70.7% vs 10.3%, P < 0.05). (2) MTA1 protein had significantly higher expression in grade III-IV, low-differentiated, and axillary lymph node metastatic breast cancer tissues than those at grade I-II, high or middle differentiated, and without axillary lymph node metastasis (P < 0.05). Conclusion: There is a positive association between the expression of MTA1 and clinical stage, histological grade, and lymph node metastasis of breast cancer. MTA1 is a metastasis-facilitated protein and can be used as a prognostic marker for detection of recurrence and metastasis of breast cancer.

Objective To study the differential proteome of kidney between lupus nephritis mouse and normal mouse.Methods The proteins of kidney were separated by two-dimensional gel electrophoresis(2-DE).The gels stained by silver were scanned by ImageScanner and analyzed by PDQuest software.Results About 573?52 and 658?43 protein spots were found in the three maps of control group and LN group respectively;the match ratio was 83% and 87% respectively.One hundred and fourteen spots were found increased that showed a two fold increase as comparing to control group.Conclusion A significant difference in protein expression of LN mouse kidney was found and may be related to the pathogenesis of LN.

Objective To explore the clinical and MR imaging features of myxoid liposarcoma.Methods Clinical and MR imaging data of 7 patients with histologically confirmed myxoid liposarcomas on extremities were retrospectively analyzed.The age of the patients ranged from 41 to 59 years with a median age of 51 years.Results Three tumors occurred in thigh,two in calf,one in foot and one in shoulder.Six tumors were situated deeply,and one was superficial.On T_1-weighted images,all 7 tumors showed predominant isointense or slightly hypointense signals relative to muscle,with 6 having lacy,linear or amorphous loci of high signal intensity.The major portion of each tumor displayed hyperintense signals compared with fat on T_2-weighted images.Following the injection of Gd-DTPA,all tumors showed inhomogenous and strong enhancement.All tumors had septa and were well defined without obvious surrounding edema and invasion of the adjacent bones.Conclusion Myxoid liposarcomas usually show predominant isointense or slightly hypointense signals relative to muscle on T_1-weighted images and hyperintense signals relative to fat on T_2-weighted images.The fat components within the tumors may be identified as linear,lacy or amorphous foci of high signal intensity on T_1-weighted images.The contrast enhancement of the mvxoid liposarcomas is usually pronounced and heterogeneous.

Objective To evaluate the value of magnetic resonance imaging features in differentiating soft tissue lipoma from well-differentiated liposarcoma.Methods MR images of 30 patients with histologically verified fat-containing tumors(22 lipomas and 8 well-differentiated liposarcomas)were retrospectively reviewed.Well-differentiated liposarcomas and benign lipomas were compared in terms of the size of the lesion,percentage of adipose component,number of thick septa,nodular and(or)patchy nonadipose component,contrast enhancement patterns and the margin characters of the lesion.Results The mean size of lipomas[(64?35)mm]was significantly smaller(t=4.263,P

Aim To explore the proteomics mechanism of the differentiation induction effect of 4-amino-2-trif-luoromethyl-phenyl retinate(ATPR)on human leukemi-a K562 cells. Methods Human leukemia K562 cells were incubated with the same concentration (1 × 10 - 6 mol·L - 1 ) of ATPR or ATRA for 48 hours. The total cell proteins were collected, purified and digested by trypsin, solid phase extraction, and the peptides were detected by ESI-LC-MS / MS. The difference of the pro-tein expression between the cells treated with ATPR and ATRA was compared by using the Discoverer Pro-teome 1. 2 software, and the molecular function, the biological process and other information of those pro-teins were analyzed based on the DAVID, KEGG, STRING databases. Results 120 specific proteins were identified only in the ATPR group, 143 only in the ATRA group, and 422 other proteins in both groups. Results of DAVID analysis showed that ATPR-induced specific proteins were mainly involved in 39 biological processes of proteins and macromolecules metabolism, protein transport and localization and so on. Results of KEGG analysis revealed that ATPR-in-duced proteins participated in signal pathways, mainly metabolic pathways, PI3K-Akt signal pathway, TGF-beta signal pathway and other pathways in cancer. String protein interaction network analysis displayed that ATPR-induced proteins, like EIF3A, EIF6, RPL3, RPL8, RPL13, RPL7A, RPL21, RPS3, RPS14, NACA, BTF3, NHP2L1, PPP2CA proteins had direct interactions with more than or equal to 10 associated proteins. Conclusion The differentiation induction effect of ATPR on K562 cells might be as-cribed to the ATPR-induced proteins interaction net-work and the specific central proteins it induced, which are involved in the regulation of cell prolifera-tion, differentiation and apoptosis.

Peliosis hepatis is a rare benign hepatic vascular disease.There is the lack of specific clinical features and preoperative diagnosis.A patient with intermittent liver area pain was admitted to the Third Central Hospital of Tianjin in April 2014.The patient with space-occupying lessions of the right lobe of liver was preliminarily diagnosed as with hepatocellular tumor or vasogenic tumor by computed tomography and B ultrasound examinations and then received liver resection combined with cholecystectomy.The result of postoperative pathological examination confirmed peliosis hepatis with adenomatous hyperplasia of liver cells.The patient was followed up till October 20,2014 without recurrence.

0.05) . Conclusions The effect of early-stage of breast cancer treatment by breast-conserving therapy plus other adjunct therapies is satisfactory . It can be as the first choice for the treatment of patients with early-stage of breast cancer.