Objective To investigate the clinical effect of prednisone and zinc preparation combined with 2％ minoxidil in the androgenetic alopecia. Methods Ninety-eight patients with the androgenetic alopecia were randomly divided into two groups, each group was 49 patients. The treatment group received prednisone and zinc preparation oral solution combined with 2％ minoxidil, the control group received only prednisone and zinc preparation oral solution. The each course of treatment was 3 months, the effect was observed after 1-4 courses of treatment. Results The curative rate of the treatment group and control group were 10.2％( 5/49 ) ,36.7％ (18/49) and 6.1％ (3/49) ,22.4％ (11/49) respectively in 3 and 6 months after treatment, there were no significant difference between two groups (P ＞ 0.05 ). The curative rate of the treatment group and control group were 77.6％ (38/49), 91.8％ (45/49) and 42.9％ (21/49 ), 59.2％(29/49) respectively in 9 and 12 months after treatment, there were significant difference between two groups (P＜ 0.05). Conclusion On treating the androgenetic alopecia, prednisone and zinc preparation combined with minoxidilis effective and safe.

BACKGROUND: If the generative cell DNA of each grade is damaged or mutated, it is possible to transmit to the further generations by means of fertilized ova. As a traditional antiepileptic, the mutagenic effects of phenytoin sodium on so matic cells had been confirmed by many researches, but it is still unknown whether phenytoin sodium has the mutagenic effects on generative cells. OBJECTIVE: To detect the mutagenic effects of phenytoin sodium on human sperm chromosomes. DESIGN: In present study we measured human sperm chromosomes in vitro by means of randomized control observa tion. SETTING: Mental Health Center of the First Affiliated Hospital, Chongqing University of Medical Sciences. MATERIALS: Phenytoin sodium was purchased from Sigma Company. The human sperms were collected from the healthy males who had not contacted with any physicochemical mutagen within recent 6 months. Ova were collected from female golden hamsters of 6-8 weeks, which were purchased from Shanghai Institute of Family Planning Science. Medium for washing sperms and ova was the BWW solution containing 0.3% human serum albumin (HSA); Medium for capacitation was the BWW solution containing 3.5% HSA; Medium for post-fertilization was an oval one containing 10% hamster serum. METHODS: After washing, centrifugation and capacitation, the sperms were made into suspension and dispended into 5 centrifuge tubes (5 mL each): Bleomycin A5 (40 mg/L) was added in the first tube as positive control group, phenytoin sodium (10, 20 and 40 mg/L) were added in three tubes respectively, another tube did not contain any reagent as blank control. Hamster ova without pellucid zone were prepared, and equally divided into five portions, which were mixed with the above-mentioned sperms in the five groups respectively, so as to make the hamster ova fertilize, finally human sperm chromosomes were prepared with the fertilized hamster ova. The rate of chromosomal structural aberration (rate of aberrant sperm) and number of chromosomal breakages were examined. We examined the rate of chromosomal structural aberration (rate of aberrant sperm) and number of chromosomal breakages. The effects of phenytoin sodium of three different concentrations on human sperm chromosomes were detected in vitro, and the results were compared with those in the positive control group and blank control groups. MAIN OUTCOME MEASURES: The structural aberration of sperm chromosomes, rate of aberrant sperm and number of chromosomal breakages were observed. RESULTS: ① Chromosomal structural aberration: The structural aberrations of sperm chromosomes including the chro mosomal breakage, monome breakage, fragments, crossing-over aberration, double centromere and ring-like chromo somes were observed in the phenytoin sodium groups, positive control group and blank control group, especially in the phenytoin sodium 40 mg/L group and positive control group. ② Rate of aberrant sperms and number of chromosomal breakages: The rate of aberrant sperms and number of chromosomal breakages were higher in the phenytoin sodium groups and positive control group than in the blank control group, but there was the significant differences between phenytoin sodium 40 mg/L group and positive control group (P ＜ 0.005, P ＜ 0.05-0.01). CONCLUSION: Phenytoin sodium has obvious influence on the structural aberration of human sperm chromosomes and may have mutagenic potential to human sperm cells.

Objective To compare the anti-inflammatory effects of different dosage forms of Xiaoerpingchuan. Methods Animal models such as mice ear-swelling induced by Dimethylbenzene, rat hindpaw edema elicited by Carrageenan, rat granuloma provoked by cotton-ball and animals were prepared for observing the anti-inflammatory effects of granules and boiling prescriptions of Xiaoerpingchuan. Results Granules prescription inhibited inflammation in all the models, boiling prescription inhibited the inflammatory action only in the model of rat hindpaw edema. Conclusion Both granules and boiling prescriptions of Xiaoerpingchuan had anti-inflammatory effects, and the former was better than the lattern in some experiments.

Alternariol monomethyl ether(AME) was isolated, purified and crystallized from the extracts of the corn inoculated with Alternaria alternata which was separated from the contaminated rice in the high incidence area of esophageal cancer, Lixian County. AME is one of the major metabolic products of A. alternata. In this paper, the mutagenic potency of AME was detected with the V_(79) Chinese hamster cells. The experimental results showed that with or without rat liver microsomes (S-9), AME could induce 6-Thioguanine resistant (6-TG~r) mutation in V_(79) cells and their dose-effect curves were similar. The mutagenicity of AME without S-9 was stronger than that of AME with S-9. So it is considered that AME is a direct mutagen which doesn' t need to be activited metabolically by S-9. AME may play an important role in the etiology of esophageal cancer.

The optimal contition of inducing the primary antibody by incubating sheep red blood cells(SRBC)with human tonsil cell was investigated in vitro. The proper cell density in culture was found to be 0.5—1.0 10~7 nuclear cells/ml. More antibody producing cells(plaque-forming cells,PFC)could be induced by add- ing both calf and horse serum in the culture than by adding only either one of them. Using proper dosage of insulin,lipopolysaccharide(LPS)or thymidine and hypoxan- thine could also increase the number of PFC.The rate of PFC against SRBC induced in the culture was estimated to be about 100～300 PFC/10~6 nuclear tonsil cells.

Serum concentrations of 25-OHD and PTH were determined in 71 normal subjects in Beijing both in December 1983 and in July 1984 using the methods of CPBA and RIA respectively. The results showed that the mean values of 25-OHD were 13.2?3.8 and 18.9?6.5ng/ml (M?SD, P

In this study, the results of 31 cases of hyperlipidemia treated as the study group by integrated and Western traditional medicine were compared with those of 30 cases treated as the control group by western medicine. The effect of decreasing the level of cholesterol and triglycerides in blood in the study group was significantly better than that in the control group. There fore, our therapy is useful for the clinical treatment of hyperlipidemia.

The article introduces a micro colorimetric method of iron nitrate-mercury Ⅱ thiocyanate to quanfitate chloric ion in serum.It is easy to operate, Its linear relationship, exactitude and developing colour stability are all very good. It is suitable for the application of the spectrometer and the auto analysis instrument. The experiment shows that the best wave length 535 nm can be used to dispel effectively the interfering of thc jaundice (hepatogenous) pigment of the hemocytolysis (hematogenous pigment This method makes use of less blood, and it is rapid, exact. So it is suitable for the batch examination examples in clinical biochemistry laboratory. To spread the method the efficacy can be raised effectively.

Chronic inflammation of intestine mucosal has been considered as a critical component of colorectal cancer development.Recently,the studies based on high throughput sequencing of colorectal cancer patients released a potential molecular subtype of colorectal cancer,characterized by wound healing and innate immune genes.It suggests that a probably mechanism described by a subset of cells that may be dependent on inflammatory cells and cytokines of tumor development,extremely expanding our insight of cell malignant transformation,and highlight the anti-inflammation treatment.This article will review the normal mechanism of intestine mucosal renewal,wound healing and malignant transformation.

Objective:To study the effect of hysteroscope electrotomy for treating infertile women with uterine septum.Methods: 64 patients with uterine septum were divided into observation group (25cases) and control group (39cases) according to different operation method. Patients of observation group received the combination mode of hysteroscope and laparoscope while the patients of control group received hysteroscope electrotomy. And thenthe effects of the two surgical methods for pregnancy outcome of the patients of the two groups were compared. Results: In 22 patients with complete uterine septum, the postoperative pregnancy rate was 59.09％ which was significantly higher than preoperative pregnancy rate (18.18％) (x2=4.539,P<0.05). The postoperative rate of normal childbirth in term was 31.82％ which was significantly higher than preoperative outcome (0％) (x2=8.324, P<0.004). In 42 patients which incomplete uterine septum, the postoperative pregnancy rate was 73.81％ which was significantly higher than preoperative pregnancy rate (33.33％) (x2=13,832,P<0.05). The postoperative rate of normal childbirth in term was 54.76％ which was significantly higher than preoperative outcome (0％)(x2=31.672, P<0.004).Conclusion: Hysteroscope electrotomy is significant in the treatment for patient with uterine septum, and it has series of advantages of minimally invasive surgery including small wound, less blooding, fast prognosis and others. Therefore, it is worthy in the clinical promoting.