ObjectiveTo observe the effect of acupoint application withWu Zhu Yu(Fructus Evodiae) powder to Yongquan (KI1) on the sleep quality before orthopaedic operation.MethodAccording to the principle of randomization and voluntary participation, the participants were divided into an observation group and a controlgroup, 41 in each group. The observation group was intervened by acupoint application withWu Zhu Yu(Fructus Evodiae) powder to Yongquan, and thecontrolgroup was by regular nursing care. The Pittsburgh Sleep Quality Index (PSQI) and subjective sleep evaluation were compared between the two groups.ResultThe total effective rate was 95.1% in the observation group versus 53.7% in the control group, and the observation group was significantly better than the control group in comparing the total effectiverate (P<0.05).ConclusionApplication withWu Zhu Yu (Fructus Evodiae) powder to Yongquan can improve the sleep quality before orthopaedic operation, and it’s easy-to-operate and safe, with a good compliance.

In order to meet the health needs of people, China’s health service system needs continuous reforms and adjustments. Health needs, equity and efficiency, quality of service, and the development history and current situation of the health service system are the four main dimensions to be considered during the building process of the system. This paper argues that building a people-centered health service system is the direction, and it describes its connotation from five different aspects, including human care, interrelated interests, primary health care-oriented, continuous integration, and conditions for support. This paper proposes the strategies and paths to build a people-cen-tered health service system, including to promoting the quality of primary health care, integrating health service sys-tems, and strengthening government’s responsibility.

Objective To observe and investigate the effect of esomeprazol in the elderly patients with gastric ulcers and influence for the pepsinogen,cAMP and cGMP.Methods 86 elderly patients with gastric ulcers in our hospital from June 2015 to August 2016 were selected as the research object,and they were randomly divided into control group 43 cases and observation group 43 cases, the control group were treated with omeprazole,the observation group were treated with esomeprazol,the total effective rates,symptom score and pepsinogen, cAMP, cGMP levels before and after the treatment of two groups were compared.Results The total effective rates of observation group at different time after the treatment were higher than those of control group,the symptom scores after the treatment were all lower than those of control group ,the serum pepsinogen were all lower than those of control group,the plasma cAMP and cAMP /cGMP were lower than those of control group,the plasma cGMP were higher than those of control group, (P<0.05), the differences were all significant.Conclusion The clinical effect of esomeprazol in the elderly patients with gastric ulcers is better,and it can significantly improve the expression situation of pepsinogen, cAMP and cGMP of patients, so its application value in the elderly patients with gastric ulcers is higher.

Alternariol monomethyl ether(AME) was isolated, purified and crystallized from the extracts of the corn inoculated with Alternaria alternata which was separated from the contaminated rice in the high incidence area of esophageal cancer, Lixian County. AME is one of the major metabolic products of A. alternata. In this paper, the mutagenic potency of AME was detected with the V_(79) Chinese hamster cells. The experimental results showed that with or without rat liver microsomes (S-9), AME could induce 6-Thioguanine resistant (6-TG~r) mutation in V_(79) cells and their dose-effect curves were similar. The mutagenicity of AME without S-9 was stronger than that of AME with S-9. So it is considered that AME is a direct mutagen which doesn' t need to be activited metabolically by S-9. AME may play an important role in the etiology of esophageal cancer.

BACKGROUND:Degenerative lumbar scoliosis patients wil appear to have abnormal changes in various cytokines in the serum of the body, which is closely related to patient’s pathogenesis.
OBJECTIVE: To monitor the changes in the serum levels of tumor necrosis factor-α, interleukin-6 and interleukin-1β during the onset of degenerative lumbar scoliosis.
METHODS: Sixty-six patients with degenerative lumbar scoliosis were enrolled as observation group, and according to Schwab evaluation system, there were 16 cases of type I, 32 of type II, and 18 of type III. Additionally, 60 healthy persons undergoing homochronous physical examination were extracted as control group. Blood samples were taken at preoperative 1, 2, 3 weeks and at postoperative 1, 2, 3, 4, 5, 6 weeks to determine serum levels of tumor necrosis factor-α, interleukin-6 and interleukin-1β as well as to observe the relevant changes.
RESULTS AND CONCLUSION:In the observation group, the levels of tumor necrosis factor-α, interleukin-6 and interleukin-1β were increased gradualy at 1-3 weeks before treatment, and then showed a decrease trend at 6 weeks after treatment. Compared with the control group, in the observation group, the levels of tumor necrosis factor-α were significantly higher at 1-4 weeks after admission (P < 0.05); the levels of interleukin-6 and interleukin-1β were significantly higher at 1-5 weeks after admission (P < 0.05). These findings indicate that the levels of tumor necrosis factor-α, interleukin-6 and interleukin-1 reached the peak at 1 week before treatment, and then gradualy decreased. Changes in the levels of tumor necrosis factor-α, interleukin-6 and interleukin-1β have an important role to assess disease development, which can be used to determine the severity of inflammation based on which we can select the appropriate treatment.

Objective To explore the evaluation of tumor specific growth factor (TSGF) ,alpha‐fetoprotein (AFP) and vascular endothelial cell growth factor(VEGF) levels in therapeutic effect of primary hepatic carcinoma (PHC) .Methods Serum TSGF , AFP and VEGF levels were detected on 1 d before treatment ,4 ,28 d after treatment in 75 patients with PHC .The relation between serum VEGF with AFP on 1 d before treatment was analyzed .Results The levels of serum TSGF ,AFP and VEGF on 4 ,28 d after treatment in 75 cases of PHC were significantly lower than those on 1 d before treatment ,the difference was statistically significant (P< 0 .05) .The VEGF level had no statistical difference between the patients with AFP ≥ 400 ng/mL and the patients with AFP <400 ng/mL(P> 0 .05) .Conclusion TSGF ,AFP and VEGF can serve as the indicators for evaluating PHC prognosis .

Bronchiolitis is the most common lower respiratory infection disease in infants and children.The main pathogen of bronchiolitis is respiratory syncytial virus (RSV),which is closely related to the occurrence of asthma.By far,the pathogenesis of bronchiolitis has not yet been fully elucidated.There is also no specific treatment for bronchiolitis.Recent studies have found that mesenchymal stem cells has strong advantages in reducing airway inflammation,airway hyperresponsiveness and airway damage repair and other aspects.This review mainly summarizes the association between bronchiolitis immunological mechanisms and mesenchymal stem cells.

Through years of experience to design , build, debug and operate the automatic control system in laboratory animal facilities , the author expounds the detailed effect of such system , analyzes the problems and causes , and finally provides some advice to improve the system .

Objective To compare the clinical effects of glass ionomer cement and enamel binder therapy on malocclusion deformity.Methods 68 orthodontic patients were divided into observation group and the control group,34 cases in each group.The observation group was given glass ionomer cement bonding,the control group was given enamel adhesive bonding.All patients in the two groups received multiloop edgewise arch appliance orthodontic treatment.The clinical outcomes were compared between two groups.Results After treatment follow-up,the incidencerate of brackets off,demineralization and caries in observation group was significantly lower than the control group(x2 =3.486,2.938,3.486,all P ＜0.05).The satisfaction of patients in the observation group after treatment was significantly higher than control group (x2 =2.047,P ＜ 0.05).The incidence rate of complications and adverse reactions during treatment showed no significant difference between the two groups (P ＞ 0.05).Conclusion Glass ionomer cement has good bonding effect in orthodontic treatment,and can minimize the brackets off,demineralization,and easy to operate,safe and reliable,which worthy of clinical applications.

BACKGROUND: If the generative cell DNA of each grade is damaged or mutated, it is possible to transmit to the further generations by means of fertilized ova. As a traditional antiepileptic, the mutagenic effects of phenytoin sodium on so matic cells had been confirmed by many researches, but it is still unknown whether phenytoin sodium has the mutagenic effects on generative cells. OBJECTIVE: To detect the mutagenic effects of phenytoin sodium on human sperm chromosomes. DESIGN: In present study we measured human sperm chromosomes in vitro by means of randomized control observa tion. SETTING: Mental Health Center of the First Affiliated Hospital, Chongqing University of Medical Sciences. MATERIALS: Phenytoin sodium was purchased from Sigma Company. The human sperms were collected from the healthy males who had not contacted with any physicochemical mutagen within recent 6 months. Ova were collected from female golden hamsters of 6-8 weeks, which were purchased from Shanghai Institute of Family Planning Science. Medium for washing sperms and ova was the BWW solution containing 0.3% human serum albumin (HSA); Medium for capacitation was the BWW solution containing 3.5% HSA; Medium for post-fertilization was an oval one containing 10% hamster serum. METHODS: After washing, centrifugation and capacitation, the sperms were made into suspension and dispended into 5 centrifuge tubes (5 mL each): Bleomycin A5 (40 mg/L) was added in the first tube as positive control group, phenytoin sodium (10, 20 and 40 mg/L) were added in three tubes respectively, another tube did not contain any reagent as blank control. Hamster ova without pellucid zone were prepared, and equally divided into five portions, which were mixed with the above-mentioned sperms in the five groups respectively, so as to make the hamster ova fertilize, finally human sperm chromosomes were prepared with the fertilized hamster ova. The rate of chromosomal structural aberration (rate of aberrant sperm) and number of chromosomal breakages were examined. We examined the rate of chromosomal structural aberration (rate of aberrant sperm) and number of chromosomal breakages. The effects of phenytoin sodium of three different concentrations on human sperm chromosomes were detected in vitro, and the results were compared with those in the positive control group and blank control groups. MAIN OUTCOME MEASURES: The structural aberration of sperm chromosomes, rate of aberrant sperm and number of chromosomal breakages were observed. RESULTS: ① Chromosomal structural aberration: The structural aberrations of sperm chromosomes including the chro mosomal breakage, monome breakage, fragments, crossing-over aberration, double centromere and ring-like chromo somes were observed in the phenytoin sodium groups, positive control group and blank control group, especially in the phenytoin sodium 40 mg/L group and positive control group. ② Rate of aberrant sperms and number of chromosomal breakages: The rate of aberrant sperms and number of chromosomal breakages were higher in the phenytoin sodium groups and positive control group than in the blank control group, but there was the significant differences between phenytoin sodium 40 mg/L group and positive control group (P ＜ 0.005, P ＜ 0.05-0.01). CONCLUSION: Phenytoin sodium has obvious influence on the structural aberration of human sperm chromosomes and may have mutagenic potential to human sperm cells.