0.05). The maximum color differences ( ?E) in the selected firing cycles were 2.279 8 and 1.389 4 for GI Ⅱand Vita In Ceram Alumina groups respectively, the maximum ?E between the two groups was 2.295 6, which fell into the clinically acceptable threshold for color differences. Conclusion: Color stability of all ceramic restorations with GI Ⅱand Vita In Ceram Alumina as substructure can be guaranteed after repeated firing of veneering porcelain up to 10 times.

Objective To investigate the safety and feasibility of early removal of chest tubes after video-assisted thoracoscopic (VAST) lobectomy for lung cancer. Methods A retrospective study was performed based on the clinical data of sixty consecutive patients who underwent VATS lobectomy plus mediastinal lymph nodes dissection for lung cancer from October 2013 to September 2014 in a single center. Thirty patients were enrolled into the early removal management group (chest tubes removal when the drainage volume is less than 300 mL/d), while the other thirty patients were enrolled into the traditional management group (chest tubes removal at the drainage volume less than 100 mL/d ). Results Patients who underwent early removal management had a shorter time of chest tubes removal and postoperative hospital stay compared to the patients in the traditional management group [(2.10 ± 0.99) d vs. (3.83 ± 1.41) d, t = 5.485, P = 0.000; (7.97 ± 1.54) d vs. (9.20 ± 2.01)d, t = 2.669, P = 0.010)]. No statistically significant differences were observed among the drainage volume, postoperative complications and symptoms on fifteen days post-operation. No complication occurred on thirty days post-operation. Conclusions Early removal of the chest tubes after VATS lobectomy ,when the drainage volume is less than 300 mL/d,is safe and feasible. This leads to a shorter length of hospital stay without addtional risk of early postoperative complications.

Objective To investigate the therapeutic effect of acupuncture combined with umbilical ring point ginger-partition moxibustion for ulcerative colitis of spleen-kidney yang deficiency type, and to observe its influence on the related inflammatory factors. Methods Sixty qualified cases were randomly divided into treatment group and control group, 30 in each group. Both groups were given oral use of live combined bifidobacterium, lactobacillus and enterococcus capsules, and the treatment group received acupuncture and umbilical ring point ginger-partition moxibustion together. After one treatment course ( four weeks) , the therapeutic effect was evaluated, and the changes of serum levels of related inflammatory factors of tumor necrosis alpha (TNF-α) and interleukin 8 (IL-8) were observed. Moreover, the safety was monitored. Results(1) The total effective rate was 86.67% in the treatment group and 66.67% in the control group, the difference being significant between the two groups (P<0.05). (2) The serum TNF-α and IL-8 levels were decreased in both groups after treatment (P<0.05 or P<0.01), and the decrease in the treatment group was superior to that in the control group ( P<0.05). ( 3) No obvious reverse action was found in the two groups. Conclusion Acupuncture combined with umbilical ring point ginger-partition moxibustion is effective and safe for the treatment of ulcerative colitis of spleen-kidney yang deficiency type.

Objective To explore the expression of growth associated protein-43(GAP-43)in the inferior colliculus in rats following bilateral cochlear ablation.Methods 35 healthy adult SD rats were randomly divided into 7 groups:3,7,14,21,28,90 days following bilateral cochlear ablation and the sham operation group as the control group.The immunohistochemistry was used to observe the expression of GAP-43 after bilateral cochlear ablation in each group.Results GAP-43 was expressed in each group.The GAP-43 expression in bilateral cochlear ablation group were higher than that in the control group.The expression increased at 3 days,followed by a constant increase at 7 to 14 days with the peak on day 14.On day 21 the GAP-43 expression began to decrease.The mean gray level of GAP-43 in all the staining of groups had statistic significance compared to the control group except the group of day 90.However,in all the 7 groups,the mean gray level between the left and right ears had no statistic significance.Conclusion The change of GAP-43 expression in the inferior colliculus following bilateral cochlear ablation reflects synaptic plasticity in inferior colliculus the neurons.

Objective To investigate the effect of remifentanil pretreatment on lipid peroxidation following acute myocardial ischemia/reperfusion (1/R) in rabbits. MethodsForty healthy adult rabbits of both sexes weighing 1.5-2.5 kg were randomly divided into 5 groups (n = 8 each): group control (group Ⅰ ); group I/R(group Ⅱ ); group morphine pretreatment + I/R (group Ⅲ ); group remifentanil (group Ⅳ ) and group remifentanil pretreatment + I/R (group Ⅴ ). The animals were anesthetized with intraperitoneal 2％ pentobarbital 45 mg/kg and were mechanically ventilated after tracheal intubation. PET CO2 was maintained between 35-45 mm Hg. Myocardial I/R was induced by iv pituitrin 2.5 U/kg in group Ⅱ , Ⅲ and Ⅴ. In group Ⅰ and Ⅳ normal saline 0.3 ml/kg was injected iv instead of pituitrin. In group Ⅲ morphine 3.3 mg/kg was injected iv at 30 min before iv pituitrin. In group Ⅳ and V remifentanil was infused at 3.3 μg· kg-1 ·min-1 for 30 min before iv normal saline and pituitrin.Venous blood samples were taken before (baseline) and at 24 h and 48 h after iv pituitrin for determination of serum cTnI concentration. The myocardial specimens were taken at T3 after blood sampling for microscopic examination and determination of SOD activity and MDA content. ResultsIntravenous pituitrin 2.5 U/kg significantly increased serum cTnI concentration and myocardial MDA content and decreased myocardial SOD activity in group Ⅱas compared with group Ⅰ . Morphine or remifentanil preatment significantly attenuated the myocardial I/R-induced changes mentioned above. Microscopic examination showed that myocardial tissue damages were ameliorated in group V as compared with group Ⅱ . ConclusionRemifentanil pretreament can attenuate acute myocardial ischemic injury by inhibiting lipid peroxidation.

BACKGROUND: The middle-school stage is the key period of personality figuring and taking shape.The mentation in this period is filled with a lot of special psychological contradictions and conflicts. Long-term psychological worry will affect the formation of perfect personality.OBJECTIVE: To understand the psychological health status in the middle-school students of Zhuang nationality and the main factors of influence.DESIGN: Randomized cluster sampling and questionnaire investigation based on middle-school students.SETTING: Department of Preventive Medicine, Youjiang Medical College of Nationalities.PARTICIPANTS: 2 000 middle-school students of 34 classes from 3 middle schools in Baise City and Longlin County were selected to attend the investigation with randomized cluster sampling method in November 2003.METHODS: The psychological health statuses of middle-school students were evaluated with symptom checklist (SCL).The list contained 90 items,including 9 factors of psychological symptom such as somatization,compulsion,sensitivity to human relations,anxiety,depression,hostility,dread,paranoia and psychosis.The grading system from 1 to 5 grades was used and the standard of the adult norm of the whole country was refered to:Factor score＜1.8 was normal;Factor score from 1.8 to 2.0 was mild abnormality;Factor score from 2.1 to 3.0 was moderate abnormality;Factor score＞3.0 was severe abnormality. Instructive words were unified.The investigative method and behavior were normalized.The questionnaires were filled in without recording the names and taken back from the spot and then compared with the norm of the whole country.MAIN OUTCOME MEASURES: The evaluated result of psychological health in middle-school students.RESULTS: 2 000 questionnaires were provided and 1 855 were valid after return with the effective rate 92.75%. ①The average scores of each factor of SCL were all higher in the middle-school students of Zhuang nationality than in the norm of the whole country. The significance of difference existed(P=0.000).②The scores of somatization,depression,anxiety,dread and psychosis were all higher in the schoolgirls than in the schoolboys.The significance of difference existed (P=0.000).③Except hostility factor,the scores of other factors were higher in the senior middle-school students than in the junior middle-school students.The difference was statistically significant(P=0.000).④The arranged sequences(from bigness to smallness) of each factor that affected psychological health of middle-school students of Zhuang nationality in Baise were depression,somatization,compulsion,anxiety,sensitivity to human relations,psychosis,paranoia,hostility and dread.The sensitivity to human relations ranked 4th and the anxiety ranked 6th in the schoolboys,but the orders of the 2 factors were just the opposite in the schoolgirls and the orders of other factors were the same in both the schoolboys and the schoolgirls.The first 3 factors all were:depression,somatization, compulsion.CONCLUNSION: The psychological health status was lower in the middle-school students of Zhuang nationality than in the norm of the whole country and the first 3 factors that affected their psychological health were:depression,somatization and compulsion. The levels of psychological health on somatization,depression,anxiety,dread and psychosis were lower in the schoolgirls than in the schoolboys. The psychological problems were more serious in the senior middle-school students than in the junior middleschool students. The levels of psychological health in middle-school students of Zhuang nationality were relatively lower and the psychological education and intervention study should be provided aiming at psychological characteristics of different population.

Objective To evaluate the role of delta and kappa opioid receptors in sufentanil preconditioning (SPC)-induced attenuation of myocardial ischemia-reperfusion (I/R) injury in rats.Methods Forty adult male Sprague-Dawley rats,aged 2-3 months,weighing 250-330 g,were randomly divided into 5 groups (n =8 each) using a random number table:sham operation group (group S),group I/R,group SPC,δ receptor antagonist naltrindole + SPC group (NTD + SPC group),and κ receptor antagonist nor-binaltorphimine (nor-BNI) + SPC group (BNI + SPC group).Myocardial I/R was induced by 30 min occlusion of left anterior descending branch of coronary artery followed by 120 min reperfusion.In group SPC,5 min infusion of sufentanil 3μg/kg was repeated 3 times at 5 min interval before myocardial ischemia.In NTD + SPC group,naltrindole 5 mg/kg was intravenously injected,at 10 before SPC.In BNI + SPC group,nor-BNI 5 mg/kg was injected intravenously at 15 min before SPC.Arterial blood samples were collected at 120 min of reperfusion for measurement of the serum cardiac troponin I (cTnI) and creatine kinase isoenzyme-MB (CK-MB) concentrations.The rats were then sacrificed and hearts removed for determination of myocardial infarct size (IS) and area at risk (AAR).IS/AAR ratio was calculated.Results Compared with S group,the serum cTnI and CK-MB concentrations were significantly increased in I/R,NTD + SPC and BNI + SPC groups,and the serum CK-MB concentrations were increased in SPC group,and IS/AAR ratio was increased in I/R group (P ＜ 0.05 or 0.01).The serum cTnI and CK-MB concentrations were significantly lower in SPC,NTD + SPC and BNI + SPC groups and IS/AAR ratio was lower in SPC and BNI + SPC groups than in I/R group,and higher in NTD + SPC and BNI + SPC groups than in SPC group (P ＜ 0.05 or 0.01).Conclusion Both κ and δ opioid receptors mediate SPC-induced attenuation of myocardial I/R injury in rats.

The main pollution of coastal sea is heavy metal and eutrophication,which resulted in degeneration of its eco-function.Pollution of heavy metal is accompanied with eutrophication in sea water.This article studied on copper absorption capacity of several bacteria strains which were isolated from the surface of macroalga Gracilaria lemaneaformis that was cultivated in copper polluted sea.We got 6 strains of epiphytic bacteria from the seaweed,Ochrobactrum anthropi,Aeromonas salmonicida,Micrococcus lylae,Corynebacterium ulcerans,Pseudoalteromonas spp,Vibrio fluvialis.Attained from the experiment of copper biosorption,A.salmonicida had a maximal biosorption capacity among them,and Vibrio minimal.Based on this trail,A.salmonicida,C.ulcerans and Vibrio were selected for next trails included optimal biosorption time,optimal pH value and effect of pre-disposal by several chemical reagents on biosorption capacity.The results showed:optimal biosorption time was between 40 minutes and 60 minutes,and optical pH value 4～5.Pre-disposal by HCl leaded to lost the absorption capacity of A.salmonicida,but was opposite by NaOH and ethanol.For C.ulcerans and Vibrio,the copper absorption capacities were increased by them.

Objective To evaluate the effect of relaxation training on sudden deafness patients with mild or moderate grade.Methods 78 patients were divided into the observation group (38 cases) and the control group (40 cases).Besides usual care and nursing,the observation group was treated with relaxation training daily in the morning and evening.The pure tone audiometry scores before and after nursing intervention and treatment was compared between two groups.Results There was significant difference in threshold level between two groups.Conclusions Relaxation training was an effective nursing intervention for sudden deafness patients with mild-to-moderate grade.

BACKGROUND:Urothelial cells are important seeding cells for urinary tissue engineering, but they are difficult to proliferate in vitro. Several studies have shown that bone marrow mesenchymal stem cells can differentiate into urothelial cells, but how these cells functions in vivo in epithelium generation after implantation, and the application of these cells in tissue engineering, are rarely studied. OBJECTIVE:To explore the isolation and proliferation of rabbit bone marrow mesenchymal stem cells that are induced into urothelial cells in combination with rabbit bladder acellular matrix to construct tissue-engineered grafts, and to assess the effect of the induced cells as seeding cells. METHODS:Twelve 8-week-old male New Zealand white rabbits were chosen to obtain bone marrow samples through tibia puncture, and to isolate bone marrow mesenchymal stem cells by density gradient centrifugation. Then the fourth or fifth generation of bone marrow mesenchymal stem cells were cultured in conditioned medium for 2 weeks, and then identified by PCR and immunofluorescence. After that, the induced cells were seeded on rabbit bladder acellular matrix to construct tissue-engineered grafts for bladder repairing. Another 12 rabbits served as control group, and urothelial cells combined with bladder acellular matrix was used for bladder repairing. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cells were successful y cultured and proliferated in vitro. After induction, PCR detection suggested that stem cellmarker (CD44) expression decreased, and epithelial cellmarker (UP1a) expression increased in the induced cells. Immunofluorescence staining demonstrated that the induced cells rather than bone marrow mesenchymal stem cells were positive for specific urothelial marker, UP1a. A stable continuous epithelial layer was observed on tissue-engineered grafts constructed by induced cells after 2 weeks, similar to the grafts built by urothelial cells. Induced bone marrow mesenchymal stem cells can differentiate into urothelial cells that can be used as seeding cells for urinary tissue engineering, which may be another choice out of urothelial cells.