OBJECTIVE: To establish a HPLC method for the determination of serum concentration of caffeic acid in rats and which was applied to the pharmacokinetic study of caffeic acid. METHODS: The concentrations of caffeic acid in rats were determined after intragastric (ig) administration or intravenous (iv) administration of 50 mg?kg-1 caffeic acid and the pharmacokinetic parameters of caffeic acid analyzed by Topfit 2.0. RESULTS: The main pharmacokinetic parameters of caffeic acid after ig administration of caffeic acid were as follows: Cmax: (0.56?0.12) ?g?mL-1; tmax: (0.18?0.04) h; t1/2: (0.67?0.12) h; ke: (1.05?0.20) h-1; AUC(0～t): (0.34?0.05) ?g?h?mL-1. The main pharmacokinetic parameters of caffeic acid via iv administration were as follows: t1/2: (0.45?0.05) h; ke: (1.55?0.18) h-1; AUC(0～t): (9.07?2.24) ?g?h?mL-1. CONCLUSION: Administered intragastrically, caffeic acid was characterized by rapid absorption, short elimination half life (t1/2) and low absolute bioavailability in rats.

Objective To investigate the influence of family support on the psychology and life quality of patients with diabetic retinopathy. Methods To survey the family support status, psychological status and quality of life by the questionnaire among 86 cases of diabetic retinopathy (DR) patients and to do the correlation analysis. Results Most of the dimension scores and total score of CLVQOL of the group of low score of family support (33 cases) were significantly lower than the group of high score of family support (53 cases). The score of somatization, compulsion, interpersonal sensitivity, depression, anxiety, paranoia, psychosis and the SCL-90 total score of family support group with low scores was significantly higher than the family support group with high scores. Conclusions The family support can help maintain and improve the quality of life and the status of mental health of patients. Health care workers should pay more attentions to the guidance and education of the DR family members of patients to enhance the awareness of timely improved family support, to ensure the provision of high quality family support and promote the quality of life of patients.

OBJECTIVE:To determinate the metabolites of ranolazine in rats by LC-MSn.METHODS:Rats were given 80 mg?kg-1 ranolazine by i.g.During 0～24 h after intragastrical administration,the sample of urine was collected and extracted by solid phase column.Extracts were determined by LC-MSn.The mobile phase consisted of acetonitrile,10 mmol?L-1ammonium acetate and glacial acetic acid at a flow rate of 0.5 mL?min-1.Fragmentation ions of ranolazine and its metabolites were determined in positive electrospray ionization by using MS,MS2 and MS3 full scans.RESULTS:Twelve phaseⅠ metabolites (O-demethylation,O-dearylation,hydroxylation,N-dealkylation and amide hydrolysis) and nine phase Ⅱ metabolites(O-glucuronidation and sulfation) were found in the sample.CONCLUSION:Ranolazine is extensively metabolized in rats.

Objective To investigate the associations between single nucleotide polymorphisms in the gene encoding human leukocyte antigen-G ( HLA-G) and the genetic susceptibility to high risk human papillomavirus ( HPV) type 18 infection in the subjects from Taizhou, Zhejiang province. Methods The genetic polymorphisms of HLA-G gene (14 bp In/Del and +3142C/G) in cervical samples collected from HPV 18-positive and healthy women were analyzed by PCR and gene sequencing technology. Statistical anal-ysis was performed by using SPSS version 16. 0. Chi-squared test was used to analyze the differences with HLA-G gene allele and genotype frequencies between healthy subjects and patients. Results Compared with healthy subjects, women with oncogenic HPV18 infection showed lower frequencies of -14 bp allele,-14 bp/-14 bp genotype and -14 bp/+3142C haplotype (P<0. 05). Moreover, lower frequencies of+3142C/C genotype were detected in HPV18-infected women with pathologically normal cervix (6. 3% vs 21. 1%, OR=0. 25, P<0. 05) and higher percentages of +3142G/G genotype were detected in women with CIN2/3 stage HPV18 infection as compared with those of the control group (68. 8% vs 35. 1%, OR=4. 06, P<0. 05). Conclusion The HLA-G gene 3′ UTR polymorphisms were closely associated with HPV18 in-fection and cervical intraepithelial neoplasia.

Objectives:To investigate the pathogenesis of Chlamydia pneumoniae (C.pneumoniae) pneumoniae with immunohistochemical staining of tissue biopsies. Methods:The Icr mice were inoculated with C.pneumoniae, strain CWL 029, by the intranasal or intravenous routes. After a single inoculation, mice were killed on the 1st, 3rd, 7th, 14th, 21st, 28th and 60th day separately. Lung specimens were obtained from the acute stage of C.pneumoniae pneumonitis and stained using a C.pneumoniae specific monoclonal antibody. Results:In the intranasal inoculation of mice, the immunoperoxidase staining of C.pneumoniae in lung tissue was positive on day 3,7,14. The positive staining of inflammatory lung tissue was not even but local. The positive expressions of C.pneumoniaewas were mainly in the alveolar macrophages, the interstitial cells and the lymphoid tissues surrounding the bronchi. After iv inoculation, a similarly changes were found but the degree was lighter than that of intranasal inoculation group. The positive expressions of C.pneumoniaewas were mainly in the alveolar macrophages and the interstitial cells. Conclusions:Immunohistochemistry is beneficial to the diagnosis of the acute stage of C.pneumoniae pneumonitis in the mice, and the pathogenesis of infection in intranasal inoculation group was more serious than that of iv inoculation group.

Objective To investigate the trait coping styles, emotional intelligence, positive mental characters and the impact of event among Chinese recruits, and the relationship among them. Methods The Trait Coping Style Questionnaire (TCSQ), Schutte's Emotional Intelligence Scale (SSEIS), Positive Mental Characters Scale for Recruits (PMCS-R), and Impact of Event Scale-Revised (IES-R) were used to assess the coping styles, emotional intelligence, positive mental characters and the impact of event in 1 837 male recruits that were divided into two groups (stress group and control group) by considering the scores of the IES-R as the screening criteria (≥35). The data were analyzed by t test, correlation analysis and multiple stepwise regression analysis. Results Totally 148 (8.1%) male recruits with stress were screened, and 163 male recruits without stress were selected using stratified random sampling according to demographic characteristics of age, education level and so on. There were significant differences between stress and control groups in the scores of TCSQ, SSEIS and PMCS-R except utilization of emotions and wisdom. Multiple stepwise regression analyses showed that the negative coping, regulation of emotion and wisdom entered the regression equation of the total score of IES-R in the stress group (F=13.395, R2=0.218, P<0.05). However, in the control group, only negative coping came into the equation of the total score of IES-R (F=22.887, R2=0.155, P<0.05). Conclusion Trait coping styles, emotional intelligence and positive mental characters can predict the impact of stress event among recruits effectively, which could provide theoretical basis for the mental health service in recruits.

OBJECTIVE:To study the metabolite and excretion of polydatin (Trans-resveratrol-3-O-glucoside,TRG) and its metabolites in Beagle dog feces,and provide experimental basis for the new drug development of TRG. METHODS:4 Beagle dogs were intragastrically administrated TRG 20％ polyethylene glycol 400 solution 50 mg/kg. LC/MS was used to identify the me-tabolites of TRG in dog feces after 12-24 h of administration,and HPLC was adopted to determine the cumulative excretion rate and excretion speed rate of the major metabolite resveratrol(TR)in feces samples after 0-12,12-24,24-36,36-48,48-72,72-96 h of administration. RESULTS:TR and two sulfate conjugates of TR were found in dog feces,and TR was a major metabolite, while TRG was undetermined. The excretion speed rate of TR in dog feces was the rapidest after 12-24 h of administration,reach-ing 9.65 mg/h. Its cumulative excretion rate was 39.3％,equivalent to 67.2％ of TRG. CONCLUSIONS:After TRG is intragastri-cally administrated to dogs,TRG was mainly excreted in the form of TR via dog feces.

Objective To explore the mediating effect and moderating effect of positive mental characters in the relationship between childhood abuse and depression among recruits.Methods 1925 recruits aged from 16 ～24 were investigated using the Childhood Trauma Questionnaire-28 item Short Form (CTQ-SF),Self-Rating Depression Scale (SDS) and Positive Mental Characters Scale for Recruits (PMCS-R) through random cluster sampling.Results The scores of emotional abuse,physical abuse,sex abuse,emotional neglect,physical neglect,depression,positive mental characters were 6.11±1.69,5.49±1.32,5.61±1.45,9.15±3.66,9.57±2.93,0.45±0.11,3.80±0.64,respectively.There were correlations among childhood abuse,depression and positive mental characters (P＜0.05).Emotional neglect,physical neglect and emotional abuse could explain 28.6％ of the total variance of depression (F=256.72,P＜0.05).The positive mental characters partially mediated the relationship between emotional neglect,physical neglect,emotional abuse and depression (mediating effect were 0.19,0.15 and 0.09,respectively),and it could only moderate the relationship between emotional neglect and depression (F=24.73,P＜0.05).Conclusion Childhood abuse not only directly but also indirectly affects depression through the positive mental characters;meanwhile the positive mental characters can change the relationship between childhood abuse and depression.

Objective To examine the impact of stressful training on recruits and relationships with adult attachment,neuroticism and negative coping style.Methods 1 962 recruits were assessed with aduh attachment scale (AAS),impact of event scale-revised (IES-R),Eysenck personality questionnaire (EPQ) and trait coping style questionnaire (TCSQ).Results (1) 67.84％ of recruits reported the varying degrees of stress,and the total average of IES-R was 1.25±0.53.The impact of training of only-child recruits (1.28±0.56) was higher than that of none-only-child (1.22±0.51) (P＜0.05).The total average of attachment close was 3.73±0.51,attachment depend was 3.24±0.53,and attachment anxiety was 2.40±0.77.(2) Attachment anxiety,neuroticism and negative coping style were all positively correlated with impact of training (r =0.384,0.570,0.543,all P＜ 0.01).Attachment close and depend were both negatively correlated with impact of training (r=-0.221,-0.251,all P＜ 0.01).(3) Neuroticism and negative coping style played a completely intermediary role between adult attachment and impact of training.(4) The multiple group analysis showed that the mediation model was adapted to the only-child or none-onlychild recruits.Conclusion Adult attachment can predict impact of training to some extent in the intermediary roles of neuroticism and negative coping style.

Objective To explore sleep quality and related psychological factors among young army cadres to provide theoretical basis for health interventions. Method A total of 1 100 young army cadres aged 18 to 34 were assessed with Pittsburgh sleep quality index (PSQI), Symptom Checklist-90 (SCL-90) and Center for Epidemiologic Studies Depression Scale by cluster random sampling. Based on CES-C scores, cadres were divided into two groups named depression group and non-depression group;850 sergeants were served as the control group. T test, chi-square test and multiple regressions were used in data analysis. Result Sleep problems were found in 13.5% of the cadres and 12.1% sergeants. General status of sleep quality of cadres was worse than sergeants' and norm's. Exercise less, physical disease and psychological problems were the risk factors. Multiple stepwise regression analysis showed that depression factors of SCL-90 had the most significantly predictive effect on sleep quality (r=0.552, Beta=0.387), followed by somatization (r=0.552, Beta=0.223), two factors could explain 32.7% of PSQI scores variation. Correlation analysis showed that depression is closely related to subjective sleep quality, sleep duration, sleep disorder and daytime dysfunction (all r>0.3). Cadres' depression (11.11 ± 6.62) was significantly lower than norm's (12.30 ± 10.39) and higher than sergeants'(8.11 ± 6.56). PSQI scores of depression group (6.05 ± 3.13) were significantly higher than non-depression group's (3.56 ± 2.69) and norm's (3.23 ± 3.12). Conclusion Depression is a major correlation factor of the sleep quality. Regular exercise, improvement of the physical and psychological health quality, and reducing depression are effective ways to improve sleep quality.