OBJECTIVE:To assess the stability of the quality control of FPIA for monitoring of CsA and MEIA for monitoring of FK506.METHODS:The quality control on the blood samples of patients receiving therapeutic drug monitoring of CsA and FK506 from June to August in 2007 were subjected to a continual assessment and analysis to investigate the stability and accuracy of the quality control.RESULTS:The mean recoveries of the low,middle and high concentrations of CsA and FK506 were 93.14%～97.65% and 97.70%～103.09% respectively,with RSD at 4.41%～5.37% and 10.44%～14.23% respectively,which accorded with the requirement for biological sample determination stated in the Chinese Pharmacopoeia.CONCLUSION:The methods of FPIA for monitoring of CsA and MEIA for monitoring of FK506 were accurate,and worth to be applied in clinical to guide individualized medication.

Objective:To explore the influencing factors of communication between pediatric junior nurses and parents of hospitalized children,and to provide basis for further improving the communication ability of junior nurses.Methods:A semi-structured interview was conducted among 10 pediatric junior nurses. Colaizzi phenomenological seven-step analysis method was used to analyze the data.NVivo10.0 software was used to manage,excavate and search text data.Results:Four themes were extracted,including self-factors,environmental factors,lack relevant training and lack of human resources.Conclusions:There are many influencing factors in the communication between nurses and parents of hospitalized children.Managers should pay attention to them by targeted training and improving communication environment, the communication ability of junior nurses can be improved,so as to improve the quality of nursing and family satisfaction.

Objective To investigate the trait coping styles, emotional intelligence, positive mental characters and the impact of event among Chinese recruits, and the relationship among them. Methods The Trait Coping Style Questionnaire (TCSQ), Schutte's Emotional Intelligence Scale (SSEIS), Positive Mental Characters Scale for Recruits (PMCS-R), and Impact of Event Scale-Revised (IES-R) were used to assess the coping styles, emotional intelligence, positive mental characters and the impact of event in 1 837 male recruits that were divided into two groups (stress group and control group) by considering the scores of the IES-R as the screening criteria (≥35). The data were analyzed by t test, correlation analysis and multiple stepwise regression analysis. Results Totally 148 (8.1%) male recruits with stress were screened, and 163 male recruits without stress were selected using stratified random sampling according to demographic characteristics of age, education level and so on. There were significant differences between stress and control groups in the scores of TCSQ, SSEIS and PMCS-R except utilization of emotions and wisdom. Multiple stepwise regression analyses showed that the negative coping, regulation of emotion and wisdom entered the regression equation of the total score of IES-R in the stress group (F=13.395, R2=0.218, P<0.05). However, in the control group, only negative coping came into the equation of the total score of IES-R (F=22.887, R2=0.155, P<0.05). Conclusion Trait coping styles, emotional intelligence and positive mental characters can predict the impact of stress event among recruits effectively, which could provide theoretical basis for the mental health service in recruits.

OBJECTIVE:To study the stability and to predicate the expiration date of betamethasone cream.METHODS:The HPLC method was used to determination the content of betamethasone,the stability test of cream was done by initial mean velocity method and strong light exposure experiment,respectively.RESULTS:The linear concentration range for betametha?sone cream was0.25～8?g/ml(r=0.9991),the average recovery rate was98.56%,RSD=0.78%;The content was highly correlated to temperature,whereas on which the light exposure did little effect.CONCLUSIONS:Betamethasone cream should be stored under room temperature and in a cool place,it is predicted that the expiration date of betamethasone cream is1year.

Objective: To evaluate the effects of milrinone on serum myocardial enzymes and malondialdehyed (MDA), a lipid peroxidation product, in the perioperative patients undergoing valve replacement. Methods: Twenty patients, scheduled for valve replacement were randomly divided into 2 groups: group A and group B. Before induction of general anesthesia , milrinone was administrated at a 30 ?g/kg bolus dose progressively in 10 min, followed by a continuous infusion of 0.5 ?g/(kg?min) in group A and the same volume of saline was administrated in group B. The mixed venous blood samples were obtained from pulmonary artery at the following time points: before induction of anesthesia (T 1), 30 min after anesthesia (T 2), 30 min after the cross clamping of aorta (T 3 ), 10 and 30 min after the removal of the aorta clamping (T 4,5 ), 30 min and 8 h after CPB(T 6,7 ), 24 and 72 h after operation (T 8,9 ). Creatine phosphokinase (CK) and creatine phosphokinase isoenzyme (CK MB) were measured with the auto biochemical analytical machine. MDA level in serum was estimated by the method of thiobarbituric acid. Results: The values of CK and CK MB were increased significantly in 2 groups from the point T 4 to T 9 ( P

Objective To explore the relationship between avoidant personality disorder and trait coping style,especially the mediating effect of positive mental characters. Methods A sample of 8 500 male recruits was assessed with personality diagnostic questionnaire?4+( PDQ?4+) , positive mental characters scale for recruits(PMCS?R) and trait coping style questionnaire(TCSQ). Results The scores of avoidant personality disorder group were lower than control group on wisdom((3.41±0.77) vs (3.76±0.74)),courage ((3.62±0.76) vs (4.05±0.78)),humanity((3.59±0.75) vs (3.98±0.72)),justice((3.64±0.75) vs (4.02 ±0.76)),temperance((3.58±0.85) vs (4.01±0.83)),transcendence((3.77±0.72) vs (4.13±0.71)),posi?tive mental characters((3.60±0.66) vs (3.99±0.62)) and trait coping style((32.74±6.71) vs (36.61± 6.23)),while negative coping style score was higher than control group((30.87±6.91) vs (22.79±6.96)) respectively,with statistical significance( all P<0.01) . Avoidant personality disorder had significantly negative correlation with positive mental characters and trait coping style( r=-0.323,-0.320,all P<0.01) ,and signif?icantly positive correlation with negative coping style ( r=0.579, P<0.01). Positive mental characters had significantly positive correlation with trait coping style( r=0.548, P<0.01) ,and significantly negative corre? lation with negative coping style( r=-0.289, P<0.01). Hierarchy regression analysis showed that positive mental characters mediate the relationship between trait coping style ( positive coping style,negative coping style) and avoidant personality disorder ( the value of mediating effect was 50.07%,6.36%) . Conclusion ?Recruits’ avoidant personality disorder affects trait coping style not only directly,but also indirectly through positive mental characters.

Objective To analyze the expression of peripheral blood CD13+CD4+CD25hi regulatory T cells (Treg cells) in patients with diffuse large B-cell lymphoma (DLBCL) and its clinical significance. Methods The expression of peripheral blood CD13+CD4+CD25hi Treg cells in 58 newly diagnosed patients with DLBCL and 30 healthy adults was detected by flow cytometry, and the relationship between its expression and the clinical indicators were analyzed statistically. Results The levels of peripheral blood CD13+CD4+CD25hi Treg cells in newly diagnosed DLBCL and healthy adults were different, with statistically significant difference [(36.37 ±11.89) % vs. (9.03 ±2.10) %, t = 7.168, P < 0.001]. The level of peripheral blood CD13+CD4+CD25hi Treg cells was significantly higher in patients with IPI score 3ˉ5 than that in patients with IPI score 0ˉ2[(44.28±10.10)%vs. (21.51±6.23)%, t=ˉ9.347, P=0.03]. The expression of peripheral blood CD13+ CD4+ CD25hi Treg cells in stages Ⅱ, Ⅲ and Ⅳ patients were (19.48 ±1.34) %, (33.98 ±8.03) % and (47.89±8.25) %respectively, and there were significant differences among three groups (F= 38.363, P<0.001). The levels of peripheral blood CD13+CD4+CD25hi Treg cells had no relationship with age, sex or LDH level (all P>0.05). Conclusion The levels of peripheral blood CD13+CD4+CD25hi Treg cells are higher in DLBCL patients, which has a close relationship between the expression of CD13+CD4+CD25hi Treg cells and clinical stage and prognosis.

Objective To explore the feasibility of the digital grading scale applied for assessment of anxiety by nurses. Methods Firstly, the digital grading scale was made, and then it was applied for patients guided by nurses to evaluate their anxiety level by themselves within 24 hours and at 3 weeks after admission. Anxiety level of the same crowd guided by doctors was also evaluated independently with Hamilton Anxiety Scale ( HAMA) by blind method. Results The correlation coefficent of the score between the digital grading scale and HAMA was 0. 794 (P<0. 01), and the score between them was positively related. Scores of the digital grading scale were compared with HAMA whose scores were divided into 5 groups according to severity level with significant differences (P <0. 01). Conclusions The digital grading scale has an obvious correlation with HAMA and it can be used to evaluate anxiety level in patients with generalized anxiety disorder.

Objective To discuss the influence of epidural labor analgesia on the initial time of lactation.Methods From August 2015 to February 2016, a total of 155 puerperas were enrolled in the study , which were divided into labor analgesia group and non-intervention group .Self-made questionnaire was used to collect the general information of puerperas and neonates and breastfeeding data .Peripheral vein blood of puerperas was sampled to detect the serum levels of prolactin and oxytocin at three points in time ( 3-4 cm of cervical dilatation , 1 h after fetal delivery and 12 h after delivery ) .Results The serum level of oxytocin in labor analgesia group was lower than that in non-intervention group at 1 h after fetal delivery (P <0.01). Compared with non-intervention group , the initial time of lactation in labor analgesia group was delayed by 5-6 h(P<0.05).The frequency and amount of formula milk feeding in labor analgesia group were more than those in non-intervention group (P<0.05).Conclusions Epidural labor analgesia delays the initial time of lactation.

Objective:To explore the organizational identity, sense of coherence, perceived social support, and work engagement of surgical nurses in a traditional Chinese medicine hospital and the relationships between them.Methods:From March to May 2022, 450 surgical nurses were selected by convenience sampling from Guangdong Provincial Hospital of Traditional Chinese Medicine for a survey with a general information questionnaire, the Organizational Identification Scale, the Sense of Coherence Scale, the Perceived Social Support Scale, and the Utrecht Work Engagement Scale-9. SPSS 24.0 was used to perform Pearson correlation analysis. AMOS 24.0 was used for structural equation modeling.Results:The nurses' scores of organizational identity, sense of coherence, perceived social support, and work engagement were (26.00±5.00), (53.58±7.50), (47.47±7.50), and (30.06±1.00), respectively. The correlation analysis revealed positive correlations between organizational identity, sense of coherence, perceived social support, and work engagement ( r=0.369-0.741, all P<0.001). Sense of coherence played a partial mediating role between perceived social support and work engagement, and the mediating effect was 0.07, accounting for 12.07% of the total effect. Organizational identity and sense of coherence produced a chain mediating effect between perceived social support and work engagement, in which the mediating effect was 0.11, accounting for 18.97% of the total effect. Conclusions:Organizational identity and sense of coherence produce a chain mediating effect between perceived social support and work engagement in the surgical nurses of the traditional Chinese medicine hospital.