Objective To investigate the influence of Fasudil on oxidative stress in patients with early-stage diabetic nephropathy. Methods 48 patients with earl-stage diabetic nephopathy were randomly divided into control group treated with Alpha-lipoic acid and treametn group with Fasudil. All patients were under strict control of blood glucose. Changes in the levels of serum cysc, urinal mALB and 8-OHdG were observed after two weeks treatment were compared between the two groups. Results Compared to pre-treatment, the content of serum cysc, urinal mALB and 8-OHdG were significantly decreased in both groups after treatment. There was no significant difference in the content of urine mAlb , CTGF and MCP-1 between the two groups before and after treatment (P > 0.05). Conclusion Fasudil may protect kidney through a mechanism by which the level of 8-OHdG in kidney is decreased and oxidative stress is alleviated.

Objective:To detect the expression of cyclin D1 protein and Rb protein in breast cancer and analyze its relationship to carcinogenesis and development.Methods:The expression of cyclin D1 and Rb protein were detected in 52 breast cancer and 20 benige breast tissues by S-P immunohistochemical method.Results:The positivity rate of cyclin D1 overexpression in the breast cancer was 34.6%(18/52),while there was a significant difference compared with that of benign breast tissues 10%(2/20),P

Objective:To determine the expansion and differentiation effect of Staurosporine on human megalaryoblastic cell line(CMK).Methods:With MTT and colony assaies.Results:Staurosporine could densitily inhibited the expansion of CMK cell,IC 50 was 50 nmol/L,Staurosporine could inhibite CMK cell at G 1 phase,then decreased the cells of S and G 2M phases,ST in 80 nmol/L improve the expression of CD41 antigen in CMK cell,induce its differentiation.Conclusion:ST as a new kind of anti-tumor facor has different mechanism with other anti-tumor drugs. [

Objective To probe the relationships between homocysteine (Hcy) and folic acid, VB6, VB12. Methods Ninety-eight diabetes mellitus (DM) patients and 31 normal persons were recruited. Fluorescence polarization immunoassay (FPIA) was used to detect Hcy, and double labelled radioimmunoassay to detect folic acid, and VB12. Results The Hcy in DM patients was decreased in folic acid(5 mg/d) group, VB12 (1.5 mg/d), group and folic acid combined with VB12 group. but not in VB6 group. Conclusion Folic acid, VB12, and folic acid combined with VB12 can reduce Hcy in DM patients,but VB6 cannot.

Objective To explore the expression and significance of MTSS1 ( metastasis suppressor 1) in hepatocellular carcinoma.Methods MTSS1 expression was detected by immunohistochemistry in hepatocellular carcinoma, liver cirrhosis and normal liver tissues.Single-factor analysis was used to study the relationship with clinicopathological factor.Correlations between MTSS1 expression and TNM stage were analyzed with Spearman rank correlation analysis.Postoperative 5-year survival was evaluated using Kaplan-Meier survival curve analysis.Results The expression of MTSS1 in hepatocellular carcinoma was higher than normal liver tissue ( U = 168.000, P ＜ 0.05), and liver cirrhotic tissue ( U = 106.000, P ＜ 0.05); MTSS1 expression was correlated with TNM stage of hepatocellular carcinoma patients, lymph vascular invasion and tumor capsule ( separately U = 259.000, 258.500, 202.000, all P ＜ 0.05).MTSS1 expression in hepatocellular carcinoma was not correlated with patients age, gender, tumor size, AFP level, and hepatitis B surface antigen.MTSS1 expression and TNM stage of liver cancer patients was negatively correlated ( rs = - 0.383 , P ＜ 0.05 ).Postoperative 5-year survival of hepatocellular carcinoma patients with MTSS1 positive expression was significantly poorer than patients with negative and weakly positive expression (respectively 34.1% and 52.3% , x2 =6.386, P ＜ 0.05).Conclusions MTSS1 high expression may play an important role in the early hepatocellular carcinoma progression, indicating a poor prognosis.

To elevate the teaching effect of clinical nutriology in adult education and be satisfied with clinic and society. The exploration is made of choosing textbook and refining teaching material,adjusting teaching methods,paying attention to cultivating practice skills and reform on exam. The results of scores of clinical nutriology and practice skills are good.

OBJECTIVE:To provide reference for the rational clinical prophylactic use of antibiotics. METHODS:The cases in whom the clinical prophylactic use of antibiotics between 2006 and 2007 in our hospital showed failure in efficacy were monitored to analyze the cause of failure,meanwhile the clinical samples were sent for culture,isolation,identification and drug susceptibility tests as well as drug resistance analysis. RESULTS:Cephalosporins,Quinolones,Penicillins,Cefhalosporins + enzyme inhibitor were more commonly used antibiotics in our hospital,with drug resistance rates at 57.21%,65.14%,68.63%,and 18.81% respectively. Among the total 240 cases monitored,the prophylactic use of antibiotics totaled 877 times,of which,388 (44.24%) were non-indicated drug use,459 (52.34%) showed drug resistance,286 (32.61%) involved improper drug choice,and 42 (17.50%) showed dual infection. CONCLUSION:The clinical prophylactic use of antibiotics in our hospital is far from perfect; therefore,it is urgent to tighten control on the standard prophylactic use of antibiotics.

Objective This study was conducted to describe job stressors of nurses employed in military hospitals and relationship between job stressors and demography characters.Method 296 employed nurses were investigated by nurse job stressor questionnaires.Result Professional and career issues were the most frequently encountered job stressor among employed nurses,the other stressors in descending order were workload and time pressure,patient care and interaction,resource and environmental problems,management issues.Job stressors of nurses could be influenced by their age,job title,et al.Conclusion The finding suggest that effective stress management strategies should be relieve job stress.

One hundred and fifty eight post-stroke patients in the recovering period were divided into intervention group (78 cases) and control group (80 cases).Patients in intervention group received home rehabilitation service provided by general practitioners (GP) for 6 months,while patients in control group received routine rehabilitation.After 6-months,the scores of self-rated health measurement scale (SRHMS) in intervention group were significantly higher than those of control group (P ＜0.01);the visiting time and frequency,medical costs and time of caregiving were decreased (P ＜ 0.01);and the satisfaction score of the patients in intervention group was 97％.The results show that home rehabilitation service can improve effectiveness of rehabilitation for post-stroke patient in recovering period.

Objective To study the protein expression of Tspan-1 gene in colorectal carcinoma,and investigate the relationship between the expression and biologic behavior of colorectal carcinoma. Methods Immunohistochemical ELIVISION two-step method was used to detect the protein expression of Tspan-1 gene in 80 cases of colorectal carcinoma,13 cases of colorectal adenoma and 27 cases of normal colorectal tissues. Results The positive expression rate of Tspan-1 in colorectal carcinoma was significantly higher than that in colorectal adenoma and normal colorectal tissues,there was significant difference in the expression among the three groups.The positive expression rate of Tspan-1 was 90% in colorectal carcinoma,23% in colorectal adenoma and 7% in normal colorectal tissues (P ＜ 0.01).The positive expression rate of Tspan-1 in poor and moderately differentiated carcinoma was significantly higher than that in well-differentiated carcinoma (P ＜ 0.01).The expression of Tspan-1 was significantly associated with lymph node metastasis (P＜0.01),and with the 5-year survival rate of colorectal carcinoma patients,with also TNM stage and the depth of tumor invasion (P ＜0.01),and while it was not associated with gender,age,location,tumor size,and type (P ＞ 0.05). Conclusion The expression of Tspan-1 gene could be as a marker predicting the prodnosis of colorectal adenocarcinoma patients,also it may play an important role in the pathogenesis of colorectal adenoma.