Objective To observe the protective effect of Mongolian medicine Carthamus tinctorius in acute liver injury induced by D-GlaN(D-galactosamine)in rat models. Methods The acute liver injury model was established by intragastric administration D-GlaN in rats. The levels of serum aspartate aminotransferase(AST),alanine aminotransferase(ALT),glutathione(GSH-Px),the content of tumor necrosis factor-α(TNF-α)in liver tissue and the apoptosis of hepatocytes were determined. Results Mongolian medicine safflower can significantly reduce the activity of serum ALT and AST and liver tissue GSH-Px,and the degree of cell apoptosis;however,no obvious change of the TNF-αcontent was observed. Conclusion The Mongolian medicine Carthamus tinctorius has protective effect in D-GlaN-induced acute liver injury rats probably via anti-oxi-dative and anti-apoptotic abilities.

Abnormal elevation and abnormal isoenzyme of non-specific esterase (?-NAE) are intimately associated with the genesis and development of cancer.Anti-GCBAP is a new kind of biological response modifier(BRM) which is separated from gastric cancer cells immunized animal spleen. As determined by clonogenic assay. Anti-GCBAP displayed highly-potent, specific cytotoxicity regarding to target cancer cells. In the present study, we have further investigated the effect of Anti-GCBAP on the ?-NAE isoenzymes in gastric cancer-bearing nude mice. The results demonstrated that in vivo adminstration of Anti-GCBAP can not only inhibit tumor growth in tumor-bearing mice, compared with control(p

Objective To explore the expressions of stromal cell derived factor 1 α (SDF-1α) and cysteine-X-cysteine chemokine receptor 4 (CXCR4) in the peripheral blood of patients with systemic lupus erythematosus (SLE).Methods Forty hospitalized SLE patients were recruited and twenty healthy volunteers were enrolled as normal controls.The percentage of CD3+CD4+CXCR4+,CD3+CD8+CXCR4+ and the plasma concentration level of SDF-1α in the control group and SLE patients were detected by flow cytometry and ELISA.The relationship between SDF-1α/CXCR4 and SLEDAI was explored.Kruskal-Wallis H,Pearson's and Mann-Whitney U test were used for statistical analysis.Results the expression of SDF-1α [329 (127,539) pg/ml] and CXCR4 [CD3+CD4+CXCR4+:4.20(2.01,6.35)％,CD3+CD4+CXCR4+:2.70(1.68,4.20)％] were significantly elevated in SLE patients when compared to the normal controls (Z=-6.277,-5.707,-4.885,respectively,all P=0.000),and were significantly increased in highly active SLE patients than less active SLE (Z=-5.414,-5.256,-5.312,P＜0.01).The expression of SDF-1α and CXCR4 in the butterfly erythema group,anemia group and proteinuria group were significantly higher than the normal group (P＜0.05).Both SDF-1αand CXCR4 were positively correlated with SLEDAI (r=0.857,0.830,0.861,respectively,all P＜0.01).Conclusion The expressions of SDF-1α and CXCR4 increase significantly in the peripheral blood of SLE patients and there is close relationship between SDF-1α/CXCR4 and disease activity,organ damage.The results of this study suggestthat SDF-1α/CXCR4 may play an important role in the pathogenesis of SLE.

Objective To design a new multifunctional surgical instrument case in order to improve the work efficiency and safety of operation in field battle. Methods Practices of operation in field battle were analyzed retrospectively and the characteristics of modern surgery were investigated. Results A new multifunctional surgical instrument case was designed and its main clinical function and operation methods were explained. Conclusion Multifunctional instrument case has advantages of advanced function and convenient operation. It has good perspective for clinical surgery applying.

Objective To explore the value of biomechanics parameter of rabbit abdominal aortic atheroma using velocity vector imaging(VVI).Methods Ten of 45 male New Zealand rabbits were chosen as normal control group randomly,the rest experimental rabbits were made atheromatous plaque model.The rabbits were examined by two-dimensional ultrasound and VVI respectively.The intima-media thickness(IMT) or thickness of plaques of abdominal aorta 1 cm from right renal artery branch were recorded.Maximum tangential velocity,strain and strain rate of IMT or plaques were measured using VVI.Then the rabbits were killed for pathological and immuno-histochemical examination.Results Based on pathology,the rabbites were divided into 4 groups:control group(group A,n=10),group of pathological endometrial thickening(group B,n=9),group of thick fibrous cap atheromatous plaques (group C,n=15) and group of thin fibrous cap atheromatous plaques (group D,n=11).The difference of plaques thickness and biochemical indicators had no statistically significant between group B and C(P>0.05),both bigger than group A and B (P<0.05).The difference of Vmax,Smax and SRmax had statistically significant each group(P<0.05).With Vmax>0.46×10-2 cm/s,Smax>0.37%,SRmax>1.415×10-2 s-1 to find the vulnerable plaques,the sensitivity were 75.0%,84.4%,84.4% respectively,specificity were 70.8%,91.7%,83.3% respectively.Conclusions VVI can identify plaque biomechanics parameter of different progression periods,which is expected to be a reliable method to find vulnerable plaques earlier in clinic.

Objective To explore the value of abdominal aorta strain reserve(SR) of rabbits during the period of early atherosclerosis using velocity vector imaging(VVI) techniques combined with nitroglycerin (NTG) vasodilation experiment.Methods Fifty-two male New Zealand White rabbits were used in this experiment.Group A(n=12) was the normal control group.Forty rabbits were made into atherosclerosis models.The SR of abdominal aortas of all rabbits were analyzed in short axis views using VVI and NTG vasodilation experiment at the forth week and the eighth week after feeding.The period of atherosclerosis into endothelial injury stage(group B) and intima thickening stage(group C) were divided.Results There were eighteen rabbits at the endothelial injury stage in group B and thirteen at the intima thickening stage in group C.Compared with control group,arterial SR values of group B and group C were significantly decreased in turn [4.13±1.21＞2.31±0.89＞1.22±0.77,Z=-2.978,P=0.003;Z=1.959,P=0.001],IMT of group C was significantly higher than that of group A(Z=-3.108,P=0.002),strain of group C was significantly lower than that of group A(Z=-2.415,P=0.016),the strain and IMT had no significant difference among three groups except group A and group C (P>0.05).A cut-off value was 3.67 of SR for diagnosing the endothelial injury aretary had a sensitivity of 70% and spectificity of 92.3% respectively obtained by the area under the ROC curve.The agreement of this diagnosis was very good.Conclusions The variable of the SR could stand for the variable of the vascular elasticity during the period of the early atherosclerosis.VVI and NTG vasodilation experiment could quantitatively assess SR of abdominal aortas of rabbits.

0.05). CONCLUSION: Domestic and foreign Rapamycin-eluting stents are safe and efficient for emergency PCI in elder patients with AMI, without biocompatibility and safety. There are no evident differences in two type stents.

OBJECTIVE To seek for an effective,rapid,economic,safety and practical cleaning method,so as to ensure the high quality of sterilization in hospital supply center.METHODS Six hundred moderately contaminated recyclable instruments were divided into three groups randomly,conventional group,experimental group A and experimental group B(two hundred sorts each).The instruments in experimental group A were put into warmed water(40 ℃) for ten minutes first,and that in experimental group B were put into multi-enzyme solution for five minutes first,and then all the instruments in three groups were put into automatic sprinkling cleaning machine to accomplish the cleaning process automatically.After cleaning process,cleaning degree of the instruments and remained blood residues were compared in three groups.RESULTS The cleaning quality in the groups A and B was significantly higher than that of the conventional group,both P

Objective To investigate the relationship between adhesion molecule CD44v6,intercellular adhesion molecule-1 (ICAM-1) and lymph node metastasis in early stage of cervical squamous cell carcinoma.Methods Seventy-four specimens of cervical cancer stage Ⅰ b1,20 specimens of normal cervical tissue and 20 specimens of cervical squamous cell in situ carcinoma were collected from Jiangxi Provincial Maternal and Child Health Care Hospital.The expression of CD44v6 and ICAM-1 in cervical tissue was detected by real-time PCR and immunohistochemistry.The lymphatic vessel density (LVD) labeled by D2-40 was detected by immunohistochemistry.The relationship of CD44v6,ICAM-1 and LVD with the differentiation degree and lymph mode metastasis was investigated.Results The positive expression rate of CD44v6 and ICAM-1 in normal cervix,cervical squamous cell carcinoma in situ,and cervical carcinoma tissues was gradually increased,which were 0,75.00％,87.84％ and 10.00％,45.00％,81.08％ respectively.Their mRNA expression amount was gradually increased,which were 0,0.24±0.02,1.02±0.11 and 0.10 ± 0.00,0.19±0.02,1.03 ± 0.10 respectively,the differences were statistically significant (P＜0.01).LVD was gradually increased in normal cervix,cervical squamous cell carcinoma in situ,and cervical carcinoma (P＜0.01).The expression of CD44v6,ICAM-1 and LVD in low differentiated cervical carcinoma tissue was higher than that in high and middle differentiated cervical carcinoma (P＜0.01).The expression of CD44v6,ICAM-1and LVD in lymph node metastasis was higher than that in non-lymph node metastasis (P＜0.01).The expression of CD44v6,ICAM-1 and LVD in cervical cancer tissue had each two positive correlation (P＜0.01).Conclusion CD44v6 plays a promoting role in the progression of cervical cancer,which with ICAM-1 and LVD synergically promote the cervical cancer development,and could be used as an effective indicator for judging lymph node metastasis and diagnosis of cervical cancer.

Objective To observe the relationship between clinical medication and the prognosis in elderly community-acquired pneumonia (CAP).Methods A prospective randomized controlled single-blind study in Tianjin Hospital Internal Medicine.A total of 160 elderly patients with CAP were selected from January 2011 and January 2015.According to the computer,patients were randomly divided into treatment group and control group,80 cases in each group.Treatment group adopted routine treatment and moderate amount of glucocorticoids in combination with a low-dose of diuretic,and the control group only received routine treatment.In two groups of patients,we observed the initial treatment effect,change in pulse oxygen saturation,the rate of invasive test,the incidence of adverse reactions,mortality rate,total hospital stay,etc.Results Compared with control group,the initial treatment was better,the improvement rate in SPO2 was increased,and the rate of invasive test was decreased in treatment group 72 hours after treatment (x2 =6.234,3.906 and 7.059,respectively,P=0.013,0.048 and 0.008).After the overall prognosis and outcome analysis,compared with control group,the percentage of patients transferred to the intensive care unit,the mortality rate was decreased (x2=4.783 and 4.737,P=0.029 and 0.030),hospitalization cost was declined,and the total hospital stay was shorted (x2 =-72.087 and-5.127,both P＜0.0001).There was no statistically significant difference in the incidence of adverse reactions such as electrolyte disorder and mental disorder(both P＞0.05).Conclusions Corticosteroids in combination with a low-dose of diuretic for the auxiliary treatment of elderly CAP can reduce the mortality rate,decrease the rate of invasive test,and shorten the duration of hospitalization,with no obvious increase of adverse events,and it help to improve the prognosis of CAP patients.