Purpose:To study the effect of interferon-? on the growth of human cervical cancer transplanted subcutaneously in nude mice.Methods:Twenty-eight nude mice transplanted subcutaneously with human cervical cancer were divided into 4 groups: control group,positive control group(DDP group),IFN-? low dose group(1.5?10 7 u/kg) and IFN-? high dose group(3.0?10 7 u/kg). Different drugs were used from day 14 after transplantation and all mice were sacrificed after 49 days. The weight of the mice and the volume of tumor were measured. FCM was used for measuring the proportion of cell cycle S+G 2 -M(%) in tumor cells. Results:The growth inhibiting rates in DDP group,IFN-? low dose group,IFN-? high dose group were 92.8%,73.8% and 93.1% respectively. Except for mice in DDP group,there was obvious increase in the weight of mice in other three groups. As for the proportion of S+G 2 -M(%),there was no difference in control group,IFN-? low dose group and IFN-? high dose group. Conclusions:IFN-? can inhibit markedly the growth of transplanted cervical cancer,which is correlated with the IFN-? dose. The mechanism of IFN-? inhibiting cervical cancer was not due to the suppression of cancer cell proliferation.

Background and purpose:Paclitaxel (Taxol,TXL) combined with cisplatin (DDP) has become the common chemotherapeutic regimen for cervical cancer. However, DDP has higher toxicities. Nedaplatin (NDP), a new platinum analogue,has been shown to have a higher antitumor efficiency against cervical cancer and lower toxicity. This study was done to investigate the antitumor effect of TXL+NDP on HeLa cells transplanted tumor compared with TXL+DDP. Methods:HeLa cells were transplanted subcutaneously in nude mice. These nude mice were randomly divided into 6 groups by sorting when tumor diameter reached 5-6 mm: Control, TXL, NDP,DDP, TXL+NDP,TXL+DDP. Tumor volume and body weight were measured during experiment. The volume ratio (T/ C) was the volume of single agent treatment group and control group and used to assess the antitumor effect, and the combination ratio (CR) was used for the combined therapy. More than 20% weight loss was defined as reaching toxicity level. Results:TXL+NDP regimen had a synergistic antitumor effect (CR=0.76) on HeLa cell transplanted tumor, with a maximum weight loss 8.3%. The tumor volume of TXL+NDP was 172?60 mm3, which was significantly smaller than that of TXL(304?55 mm3) group or NDP(356?67 mm3) group(P0.05), but had a higher toxicity with 4 mice death. Conclusion: TXL+NDP regimen has a synergistic inhibitory effect on HeLa cells and lower toxicity. The efficacy of TXL+NDP regimen needs to be confirmed in clinical trials.

Objective To analyse the prevalence, etiology, presentation, preserving ovary and progonosis of cervical cancer in women under 35 years old. Methods The clinical information of 174 patients were retrospectively analysed.Results The percentage of new cervical cancer cases from 1991 to 2001 was 1.2%, 1.2%, 4.3%, 4.2%, 4.6%, 4.5%, 7.3%, 9.0%, 10.7%, 9.4%, 10.8%, respectively( P 0.05).For patients with two or more risk factors, it was 68%, 1/7, respectively ( P

Objective To evaluate the role of lutein in preventing and slowing down age-related macular degeneration (AMD). Methods Literatures concerning lutein and AMD were collected by computerized search, manual search, expert consultations. The quality of included studies was evaluated. RevMan software 4.2was used for meta-analysis. Results Totally 9 studies were included, with a total OR of 0. 81 (range: 0. 71-0.92). Conclusion Lutein can, to some extent, prevent or slow down AMD.

Background and purpose:Cisplatin-based concurrent chemoradiotherapy(CCRT) has become the standard treatment for locally advanced cervical cancer(LACC) . But no marked survival benefi t was found for stage Ⅲ-ⅣA patients,and the high toxicity of cisplatin warrants evaluation of other chemotherapeutic agents to increase effi cacy and decrease toxicity. This study was done to evaluate the effi cacy and toxicity of paclitaxel(TXL) and nedaplatin(NDP) administered concurrently with radiotherapy followed by consolidation chemotherapy in the treatment of locally advanced squamous cell cervical carcinoma(LASCC) . Methods:29 Patients with LASCC(FIGO stage ⅡB-ⅢB) were enrolled into this study from February 5,2007 through December 10,2007. The median age was 48 years old(35-64) . Radiotherapy included external beam radiotherapy to pelvic(stageⅡB 45Gy,stage Ⅲ 50 Gy) and 192Ir brachytherapy(stageⅡB 50 Gy,stage Ⅲ 35Gy) . TXL 35 mg/m2 and NDP 20 mg/m2 were given every week for 6 weeks concurrently with radiotherapy. After one month of CCRT,TXL 135 mg/m2 and NDP 60mg/m2 were administered every 3 weeks for 4 cycles. Results:All patients completed CCRT. For the consolidation chemotherapy,25 patients received 4 cycles,2 patients 3 cycles and 2 patients 2 cycles. The clinical complete response rate was 89.7%(95%CI,78.6%-100%) ,partial response rate 10.3%(95%CI,0-21.4%) After a median follow-up of 14 months(range,8.7-18.8 months) ,the disease-free and overall survival rates were 86.2%(95% CI,73.7%-98.7%) and 96.7%(95% CI 90.2%-100%) ,respectively. One patient died due to disease progression.During CCRT,Grade 3 leukopenia occurred in 3.6%(6/169) of the cycles,Grade 3 diarrhea 0.6%(1/169) ;During consolidation chemotherapy,Grade 3 leukopenia and neutropenia occurred in 8.7%(9/104) of the cycles. There were 2 patients who developed Grade 3 late rectum toxicity. Conclusion:CCRT with paclitaxel and nedaplatin followed by consolidation chemotherapy is well tolerated and effective in patients with LASCC. A prospective randomized trial to compare this treatment strategy with standard CCRT seems to be worthwhile.

Objective To observe the effect of insulin glargine combined with methimazole treatment of senile diabetes mellitus complicated with hyperthyroidism.MethodsThe clinical data of 80 cases in People's Hospital of Haiyan from May 2014 to May 2016 were elderly diabetic patients with hyperthyroidism were analyzed.ResultsThe combined treatment group of patients with fasting blood glucose, 2h postprandial blood glucose was significantly lower than the average water alone treatment group (P<0.05), TSH level was significantly higher than that of single treatment group (P<0.05), FT3, FT4, TGAb, TMAb levels were significantly lower than the single treatment group (P<0.05), the total efficiency of 85% treatment (34/40) was significantly higher than that of single the treatment group of 40% (16/40) (P<0.05), insulin was significantly less than single treatment group (P<0.05), blood glucose time, hospitalization time were significantly shorter than single treatment group (P<0.05), the incidence of adverse reactions was 12.5% (5/40) was significantly lower than that of single treatment group 47.5% (19/40) (P<0.05).ConclusionInsulin glargine combined with methimazole in treatment of elderly diabetes mellitus complicated with hyperthyroidism was better than insulin glargine treatment alone.

Objective To investigate the involvement of mitogen-activated protein kinase(MAPK)system and hypertrophy related genes in myocardial remodeling mechanism of patients with congestive heart failure(CHF).Methods Thirty-nine patients of mitral valve disease with CHF were randomly selected and 30 cases of healthy persons were included as controls.Cardiac function parameters were measured by echocardiography.Concentration of AngⅡ in plasma and myocardial tissues were determined by radio immunoassay.Immunoprecipitation was used to assay the protein expression and phosphorylation of c-jun NH2-terminal kinase(JNK),extracellular signal regulated kinase(ERK),P 38- mitogen-activated protein kinase(P 38-MAPK)in myocardial tissues;protein expression of c-myc,c-myb,c-jun was also determined by immunoprecipitation.Results Compared to the control group,the phosphorylation of ERK1/2 was obvious in heart function class Ⅱ group(P0.05);phosphorylation of JNK was obvious in CHF groups(P0.05);there was no expression in control group.Conclusion MAPK activated by renin angiotensin system may play an important role by causing the protein expression of c-myc,c-myb,c-jun in myocardial remodeling in patients with CHF.

Objective To explore the clinical competence of nursing master of professional degree and give some suggestions for their postgraduate education. Methods A total of 19 samples of nursing masters of professional degree, nursing managers, nursing experts and clinical teachers were selected by using purpose sampling method, and semi- structured interview was adopted and the results were finally described and classified. Results The competence of the masters of professional degree came from the following five aspects: professional quality, practical ability, research ability, education ability and professional development ability. Conclusions A good professional quality is the premise of qualified for clinical work, flexible ability of practice is the basis of clinical work, solid scientific research background and the education ability is the core competitiveness, multi- dimensional professional development ability is the key to self-improvement, nursing professional degree graduate student should enhance their own comprehensive quality from the above five aspects, to be better qualified for clinical nursing work.

Objective To evaluate the impact of low dietary glycemic index on the risk of coronaryheart disease (CHD).Methods We searched the Cochrane Database of Systematic Reviews,JBI Database of Systematic Reviews,MEDLINE,EMbase,CBMdisk,and CNKI to collect cohort studies from 2000 to 2011.The quality of cohort studies was critically appraised and data were extracted by 4 reviewers independently.Meta-analysis were conducted for the eligible cohort studies using RevMan 5.0.Results Five cohort studies were included,and 128 911 samples were researched.The result of meta analysis indicated that low dietary glycemic index was a protective factor of CHD,RR value was 1.25,95％ CI was (1.13,2.15).Further more,the result was only effective to female,RR value was 1.35,95％ CI was (1.16,1.57),but not to male,RR value was 0.96,95％CI was (0.76,1.31).Conclusions Low dietary glycemic index can decrease the risk of CHD,but it is still to be proved whether it is suitable to male.

Objective To analyze the MRI data of misdiagnosed and missed diagnosed of breast lesions and their histopathological features.Methods Data from 241 breast lesions within 121 patients were recruited in this study.The data included MRI images,uhrasounds and X-ray images were retrospectively interpreted by two radiologist and each lesion was assessed according to the BI-RADS classification.The pathologic features of miss or error diagnosed lesions on MRI were analyzed.Results In 241 breast lesions (malignance 120,bcnign 121),4 lcsions were miss diagnosed on MRI.Thcy were 2 intraductal papillomatosis and 2 fibroadenoma.All was benign.Twenty three lesions were misdiagnosed on MRI.Sixteen were overestimation,including 3 chronic inflammations,3 sclerosing adenosis,2 fibroadenoma,4 fibrocystic changes with or without atypical ductal hyperplasia (ADH),2 intraductal papilloma,1 infiltration of pectoralis major muscle and 1 axillary lymphnode metastasis.Meanwhile,there were 7 lesions were underestimation.These lesions included 2 invasive ductal carcinomas,1 mucinous adenocarcinoma,2 DCIS and 1 blunt duct adenosis with ADH and focal cancerous,1 inflammatory breast cancer underwent chemotherapy.The sensitivity and specificity and accuracy of breast MRI were 95.83 ％ (115/120),72.73 ％ (88/121),84.23 ％ (203/241),respectively.MRI findings had no difference with respect to mammogram or ultrasound was 75.10 ％ (181/241).Conclusion MRI misdiagnosis and missed often occurs in smaller breast lesions,morphologic and hemodynamic malignant manifestation atypical,especially intraductal lesions.MRI diagnosis should be combined with physical examination,X-ray mammogram and ultrasound to improve diagnostic accuracy and reduce missed diagnosis.