Objective To explore the clinical diagnostic valve of serum soluble human leukocyte antigen‐G (sHLA‐G) in cervical cancer and precancerous lesion cervical intraepithelial neoplasia(CIN) .Methods The serum sHLA‐G level was detected by using ELISA and serum TA‐4 and SCC‐Ag levels were detected by using the light‐emitting electrochemical immunoassay method detec‐ting in 230 cases of cervical carcinoma ,120 cases of CIN and 30 healthy volunteers .The differences among various groups and their relationship with the clinicopathological features of cervical cancer were analyzed .Results (1) The comparison of serum sHLA‐G , TA‐4 and SCC‐Ag levels :there were statistically significant differences in serum sHLA‐G level among various groups (P=0 .000);the serum sHLA‐G level in the cervical cancer group was significantly higher than that in the healthy control group ,CIN Ⅰgroup , CIN Ⅱ group and CIN Ⅲ group (P=0 .000 ,P=0 .000 ,P=0 .002 ,P=0 .006);which in the CIN Ⅲ group was significantly higher than that in the CIN Ⅰ group and the healthy control group (P=0 .001 ,P=0 .021) .There were statistically significant differences in serum TA‐4 level among various groups (P=0 .006);the serum TA‐4 level in the cervical cancer group was significantly higher than that in the healthy control group ,CIN Ⅰ group and CIN Ⅱ group (P=0 .003 ,P=0 .008 ,P=0 .018);which in the CIN Ⅲgroup was significantly higher than that in the healthy control group and the CIN Ⅰ group (P=0 .023 ,P=0 .031) .The differences of serum SCC‐Ag level among various groups had statistically significant differences (P=0 .000);which in the cervical cancer group was significantly higher than that in the healthy control group ,CIN Ⅰ group and CIN Ⅱ group (P=0 .000 ,P=0 .001 ,P=0 .007) , and which in the CIN Ⅲ group was significantly higher than that in the healthy control group and the CIN Ⅰ group (P=0 .013 , P=0 .021) .(2) The relationship between serum sHLA‐G and pathological features of cervical cancer :the serum sHLA‐G level had no significant correlation with the age ,tumor size and pathological type (P>0 .05) ,while serum sHLA‐G was closely related with the FIGO stages and lymph node metastasis (P=0 .008 ,P=0 .031) .The serum sHLA‐G level in the FIGO stage Ⅲ and Ⅳ was significantly higher than that in the FIGO stageⅠ and Ⅱ (U=7 .125 ,P=0 .008) ,and which in the patients with lymph node me‐tastasis was significantly higher than that without lymph node metastasis (U=4 .651 ,P=0 .031) .Conclusion The detection of ser‐um sHLA‐G level can contribute to the early diagnosis and disease condition evaluation of cervical cancer and CIN Ⅲ ,thus which is likely to become a new indicator of early diagnosis of cervical cancer .But its specificity with the occurrence of cervical cancer and precancerous lesion remains to be further investigated by related research .

Objective To study the clinical significance of D‐D ,NT‐proBNP and serum lipoprotein(a) level in patients with acute ischemic stroke .Methods 150 cases of patients with acute ischemic stroke from March 2013 to March 2015 were selected as the observation group .And 50 cases of healthy physical examination were selected as control group .The levels of D‐D ,NT‐proBNP and serum lipoprotein(a) were detected in D groups .According to the nerve function defect score ,the three indexes were analyzed and studied .Results The D‐D polymer ,NT‐proBNP and serum lipoprotein(a) levels in the observation group were significantly higher than those in the control group ,the D groups were statistically significant(P<0 .05) .The levels of D‐D ,NT‐proBNP and serum lip‐oprotein(a) in the patients with severe were significantly higher than those in light and medium ,and the level of the three indexes increased gradually(P<0 .05) with the severity of the disease .Lacunar stroke with the D‐dimer ,NT‐proBNP and serum lipid pro‐tein(a) level was significantly higher than that in non lacunar group patients(P<0 .05) .Conclusion D‐D ,NT‐proBNP and serum lipoprotein(a) in the diagnosis of acute ischemic stroke patients are high clinical value ,it is worth popularizing widely .

Objective To discussion and analysis of the significance of semen and serum anti‐sperm antibodies in the test in male infertility.Methods 95casesofinfertilemalepatientsassubjectsinthisstudyfromJune2012toMay2014tocometoourhospi‐tal ,others choose to our hospital after medical investigation and no normal healthy male infertility 30 cases as controls group ,meas‐ured in blood and semen of anti‐sperm antibodies content and semen examination ,analysis and comparison .Results Semen of pa‐tients in the observation group sperm viability ,semen liquefaction time and a + b grade sperm motility percentages were lower than in the control group healthy men ,the two groups was statistically significant (P<0 .05) .Sperm viability ,semen liquefaction time and a + b grade sperm motility percentage of patients AsAb positive group were lower than AsAb tested negative for infertile pa‐tients ,the two groups was statistically significant(P<0 .05) .Patients in the observation group IL‐2 ,IL‐6 ,and the content of TNF‐αcompared with the healthy control group of men increased significantly between the two groups was statistically significant (P<0 .05) .AsAb positive serum IL‐2 group ,and IL‐6 levels and AsAb negative groups are similar ,but TNF‐αcontent than AsAb tested negative patients was significantly increased ,the two groups was statistically significant (P<0 .05) .Conclusion Anti‐sperm anti‐bodies in the blood and semen parameters have some relevance ,semen and blood detection of anti‐sperm antibodies can be used as a means of diagnosis of infertility patients ,it is worth widely recommended .

OBJECTIVE To find the active principle in Radix Scutellariae,Radix Sophorae Flavescentis and Flos Lonicerae in Gansu inhibiting yeast-like fungi of deep infection.METHODS Radix Scutellariae,Radix Sophorae Flavescentis and Flos Lonicerae were separately cleaned,toasted and grinded to fine power.Five kinds of(8 strains) yeast-like fungi that were collected and spread into 90mm Sabouraud plate.On aseptic condition,bored holes on Sabouraud plate with the drilling instrument of 6mm diameter,and filled every holes with 25.73mg fine powder of Radix Scutellariae,Radix Sophorae Flavescentis and Flos Lonicerae,and added 3 to 5 drops of distilled water in the medicinal powder.Under 35℃,the bacteria were cultured for 24 to 48 hours,and the size of bacteriostatic ring was observed.RESULTS The diameters of the bacteriostatic ring of Radix Scutellariae powder inhibiting five kinds of yeast-like fungi were separately 16.0mm,13.5mm,13.0mm,13.0mm and 10.5mm,respectively,and the diameter of Radix Sophorae Flavescentis and Flos Lonicerae powder was 0.CONCLUSIONS There is the active principle inhibiting yeast-like fungi in Radix Scutellariae,but not that in Radix Sophorae Flavescentis and Flos Lonicerae.

Objective To explore the diagnostic value of serum creatine kinase isoenzyme MB (CM‐MB) and cardiac troponin I (cTnI) for myocardial injury in children with hand‐foot‐mouth disease (HFMD) .Methods A total of 80 children with HFMD (HFMD group) and 50 healthy children (control group) were enrolled from July 2012 to June 2013 .Serum levels of CK‐MB and cTnI were compared between the two groups .Results Serum levels of CK‐MB and cTnI were (38 .10 ± 19 .50)U/L and (0 .08 ± 0 .02)μg/L in HFMD group ,which were higher than control group (P<0 .05) .In HFMD group ,the positive rate of CK‐MB was 56 .3% ,higher than the 33 .8% of cTnI (P< 0 .05) .After therapy ,serum levels of CK‐MB and cTnI were both significantly de‐creased (P<0 .05) .Conclusion Combined detection of serum CK‐MB and cTnI might be with important significance for the early diagnosis of myocardial injury in children with HFMD .

Objective To investigate the effects of low dose radiation on dendritic growth of newborn neurons in the hippocampus of young rat.Methods One month-old male rats were randomized into radiation group aind sham control group.Radiation group received whole brain irradiation at a single dose of 2 Gy.Retrovirus expressing green fluorescent protein (GFP) was used to label newborn neurons in the hippocampus through stereotaxic intracranial infusion.Immunofluorescence assays were performed to detect dendritic architecture alterations induced by irradiation at different time points.Results Compared with control group,the lengths of total dendrite and the longest dendrite significantly decreased at 2 and 4 weeks after irradiation (t =3.10,2.07,2.94,4.02,P ＜ 0.05).The branching points of new born neurons were also decreased significantly at 2 weeks post irradiation (t =2.23,P ＜ 0.05).The number of new born neurons reduced at 4 weeks post irradiation (t =8.43,P ＜ 0.05).Conclusions Low dose radiation could inhibit newborn neuron growth in the hippocampus of young rat,which may be one of the most important mechanisms involved in radiation-induced cognitive impairment.

BACKGROUND:Clinical application of neural stem cells is under exploration.Currently,the indicative differentiation of neural stem cells into specific neurons to replace lost and degenerative neurons needs to solve.OBJECTIVE:To explore the effect of 83 ku protein in rat hippocempi on inducing neural stem cell differentiation into acetylcholine esterase (ACHE) positive neurons.DESIGN,TIME AND SETTING:In vitro controlled observation of cytology was performed at the Medical College of Nantong University between October 2003 and April 2008.MATERIALS:A total of 12 SD rats,of clean grade,and SD fetal rats,aged 17 days,were provided by the Experimental Animal Center of Nantong University.METHODS:The normal hippocampi and hippocampi on the 14th day after the hippocampal fimbria transection were prepared into homogenate used for 10% native-polyacrylamide gel electrophoresis,and the differential proteins of 83 ku were electroeluted.The protein concentration was adjusted to 300 mg/L.The forebrain tissues of fetal rats were harvested and neural stem cells were isolated and in vitro cultured:blank control cells were cultured in serum-free DMEM/F12 medium;83 ku normal and 83 ku transection groups were separately cultured in serum-free DMEM/F12 medium containing 10 mg/L 83 ku protein from normal and hippocampal fimbria transection rats for 12 days.MAIN OUTCOME MEASURES:AChE histochemical staining was used to detect the differentiation of neural stem cells into AChE positive neurons.RESULTS:After 12 days of culture,there was a large amount of AChE positive neurons in 83ku transection group and their bodies were very big and the processes were abundant;The AChE positive neurons in 83ku normal group were less than 83 transection group,and their bodies were small with short processes.A few of AChE positive neurons were seen in control group.There were significant differences in number of AChE positive neurons among three groups (P＜0.05).CONCLUSIONHippocampal 83 ku protein can induce neural stem cells to differentiate into AChE positive neurons.

Objective To investigate the relationship between the nerve growth factor ( NGF ) induced hippocampal neuroregeneration and homeobox gene Lhx 8.Methods Seventy-two SD rats were divided into control group , transected group, NGF group, transected combined with NGF group after right fimbria-fornix transection and NGF intracerebroventricular injection . Real-time PCR and Western blotting were applied to detect the gene and protein expression of Lhx8 in each group.The choline acetyltransferase ( ChAT)/Lhx8 double labeled cells in subgranular zone ( SGZ) of hippocampus in each group were detected by immunofluorescence .Results The expression of Lhx8 gene and protein in the transected , NGF group and especially in the transected combined with NGF group was obviously higher than in the control group .The number of ChAT/Lhx8 double labeled cells in the NGF group and the transected combined with NGF group was obviously more than in the control group and transected group . Conclusion The hippocampal neuroregeneration which induced by NGF intracerebroventricular injection was associated with the higher expression of Lhx8.

Objective To investigate the roles of TrkA and TrkB in radiation-induced hippocampal neurogenesis impairment.Methods Fifty-six rats were randomized into radiation group and sham control group.Radiation group received whole brain irradiation at a single dose of 10 Gy.The hippocampus were separated from rats in day 1,day 3,day 14 and 1 month after irradiation.Western blot and RT-PCR were applied to detect the protein levels and mRNA levels.Golgi staining was used to observe the dendritic spine of hippocampus.Immunofluorescence was performed to detect neural precursor's proliferation.Results Compared with control group,the numbers of dendritic spine significantly decreased after irradiation and its shape change obviously.Immunofluorescence showed a significant decrease in neural precursor's proliferation comparing with control group (t =6.49,P ＜ 0.05).Protein level of TrkA expression increased (t =2.64,3.06,4.80,2.64,P ＜ 0.05),while the levels of TrkB protein expression decreased significantly (t =4.59,3.06,2.81,2.57,P ＜ 0.05).The mRNA level of TrkA expressions increased (t =4.57,3.06,5.39,5.86,P ＜ 0.05),while the mRNA level of TrkB decreased (t =14.87,11.69,4.98,P ＜ 0.05).Conclusions As a signaling pathways downstream of NGF and BDNF,TrkA and TrkB may play an important role in radiation-induced neurogenesis impairment.

Objective Modified the medium that can increase single\|clone formed rate and confirmed the single clone spheres had the multipotential of differentation. Methods We modified the medium, that is, the medium contained half of primary culture medium and half of fresh culture medium. A great deal of neurospheres dervied from a single cell were plated averagely into 24 well plates and added into the DMEM differentiation medium (containing serum). After culturing for 14 days, cultures were stained with the neuronal\| ang glial\|specific markers (MAP\|2 for neurons, GFAP for astrocytes and CNP for oligodendrocytes). Results Each 96 well plate containing half of primary culture medium generated two to three single clone spheres, in control plate containing only fresh medium generated half to one single clone sphere. After differentiation, these cell clones expressed MAP\|2, GFAP and CNP positive respectively.Conclusion\ Using half of primary culture medium can increase single\|clone formed rate and these cell clones had the multipotential of differentiation.\;[