Objective To establish a management information system for bacteria ( virus ) preservation in order to improve the management efficiency and quality of bacteria ( virus) preservation organizations to the largest possible extent . Methods Client/Server(C/S) structure was adopted to design the system with Visual Basic 6.0 and SQL Server2000 as the platform of development .A bacteria ( virus) information database was established with the E-R model and 3NF.The system was developed by face to object language program .Results Devices needed were supplied .Functional modules of bacteria ( virus ) information management , scientific research project management , user management and system maintenance were constructed .Visiting permission was installed on the platform and database to maximize the protection of bacteria ( virus ) information safety .Conclusion Bacteria ( virus ) information flow management , involving storage , classification, preservation, collection and record, is achieved.All the resources of the preservation organization are integrated.Bacteria (virus) management efficiency is increased and bacteria (virus) information safety is ensured.

Objective To evaluate the role of diagnostic and therapeutic endoscopic retrograde cholangiopancreatography(ERCP)in bile duct injury. Methods Thirty-one patients with bile duct injury from March 1999 to March 2004 were managed by ERCP. Patients with indications of endoscopic therapy were treated by endoscopic sphincterectomy(EST)and plastic stent drainage. Results Twenty-two of 31 patients with bile duct injury received endoscopic therapy . The patients included 3 cases of common bile duct transection or ligation, 13 cases of bile duct stricture, and 15 cases of bile leakage. Seven of 13 patiens with bile duct stricture received plastic stent therapy, followed for 8-20 months, 4 cases with satisfaction and 3 cases with poor outcome. Fifteen cases of bile leakage accepted EST and endoscopic nasalbiliary drainage(ENBD) , in the first time,13 cases resulted in healing of the leakage,2 cases were of no effect. Seven cases of bile duct stricture were treated with plastic stent, maintaining for 4-12 months. No procedure related complications. Conclclusions ERCP is helpful not only for diagnosing and localizing localizing bile duct injury, but also for treating it in most of patients with relatively good and safe results.

Objective To investigate the influence of Ondansetron on postoperative nausea and vomiting(PONV)in patient undergoing laparoscopic cholecystectomy with general anesthesia and the nursins care. Methods Ninety patients undergoing laparoscopic cholecystectomy with general anesthesia were randomly divided into 3 groups(n=30):low-dose Ondansetron group(O1 group)received 4 mg Ondansetron before section,high-dose Ondansetron group(O2 group)received 8 mg Ondansetron before section and the control group(C group)received normal saline.All injections were diluted to 4 ml.The number and degree of nausea and vomiting wag recorded during 24 h after operation.Results Number of patients with PONV in O1 group and O2 group were less than that of C group,P＜0.05,so was the PONV degree.No statistical difference was seen between O1 group and O2 group.Conclusion Intravenous injection of Ondansetron during operation can decrease the PONV in patient undergoing laparoscopic cholecystectomy with general anesthesia.

Objective To investigate the radiosensitizing effect of tetrandrine in human esophageal carcinoma cells (TE1) in vitro and its related mechanisms. Methods The cell proliferation was assessed by MTT assay. Colony formation was used to analyze radiosensitivity enhancement by tetrandrine in TE1cells. Western blotting was preformed to measure the cyclin B1 protein levels. Results Tetrandrine inhibited cell growth in a concentration and time depedant manner. The inhibition of proliferation was observed when cells were treated by 1.0, 5.0 and 10. 0 μg/ml tetrandrine for 24 h after irradiation ( P ＜0. 05;F= 3.09, 10.43 and 24. 00, respectively). The inhibition was more significant when cell were treated by 0. 1, 1.0, 5.0 and 10. 0 μg/ml tetrandrine for 48 h than 24 hours after irradiation (F =4. 12,12. 77, 44. 28, and 48.53 respectively ,all P ＜ 0. 01 ). The D0, Dq and SF2 decreased with the increase of the tetrandrine concentration. The maximal sensitizing enhancement ratio was 1.60 with 0. 5 μg/ml tetrandrine. Tetrandrine upregulated the expression of cyclin B1 and removed G2 / M arrest . Conclusions Tetrandrine can enhance radiosensitivity of TE1 cells. This effect may be associated with the increase of cyclin B1 expression to remove G2/M arrest.

Objective To explore the radiosensitization effect of thalidomide combined with X-ray on esophageal carcinoma TE1 cells.Methods Cell scratch assay Was used to detect the inhibition ability of different concentration of Thalidomide on cell invasion and metastasis.H3-TdR incorporation assay Was used to investigate the inhibition of DNA synthesis in TE1 cells by treated with Thalidomide singly or combination with X-rays.The colony formation assay Was used to analyze the radiosensitization of Thalidomide effect on TE1 cells.Results Thalidomide had obvious inhibition effect on TE1 cell metastasis.DNA synthesis and colony formation,which were correlated with drug concentration.The values D0,Dq and SF2 in TE1 cells were gradually decreased with thalidomide concentration increased.When the concentration of thalidomide was 100μg/ml,the SERD0 and SERDq were(1.4±0.2)and(1.5±0.1),respectively,While the concentration of thalidomide Was 1 50μg/ml,the SERD0 and SERDq were metastasis,DNA synthesis,and significantly enhance the radiosensitizing effect on esophageal carcinoma TE1 cells.

Objective To provide an experimental evidence for the clinical applications of glyceryl trinatrate(GTN)and isosorbide dinitrate(ISDN)against Coxsackievirus B(CVB)-related myocarditis.Methods Coxsackievirus B3 was propagated in HeLa cells.Virus yields were determinded by 50% tissue culture infective dosage(TCID50).BALB/c mice were attacked with 5000 TCID50 of CVB3,meanwhile,the mice were administrated with GTN and ISDN.All mice were killed at the 14th day.The myocardial tissues were harvested for histologic evaluation.Results The infection plaques in the myocardial tissues obtained from CVB3-infected BALB/c mice treated with GTN were siginificantly reduced(0.89?0.18 in GTN group and 1.25?0.22 in ISDN group)compared with that of the untreated CVB3-infected mice(P

Objective To recognize the characteristics and causes of the adverse drug reaction (ADR)of traditional Chinese patent medicine,and promote the clinical safe drug use.Methods By retrospective study,ADR reports about Chinese patent medicine collected by Shouguang adverse drug reaction monitoring center from January 1,2012 to December 31,2014 were analyzed statistically.Results A total of 479 reports in accordance with the inclusion criteria were collected,which comprised 479 patients.Of them,224 were male and 255 were female,often occurs in patients over 50 years old[53.0%(254 /479)].Chinese patent medicine involved in these reports were mainly blood -regulating formula,heat agent and formula for resuscitation,respectively 278 cases(58.0%),64 cases (13.4%),51 cases (10.6%).Intravenous administration was an important way to trigger ADR [78.5%(376 /479)],78.5% injection had adverse effects and suitability of medicine were 55.9%.Main clinical manifesta-tions were lesion of skin and its appendages[35.1%(183 /522)]and digestive system damage[18.4%(96 /522)]. Conclusion The application of traditional Chinese medicine syndrome differentiation as the primary principle,atten-tion to individual differences of patients,improve the level of rational use drugs,strengthen the proprietary Chinese medicine preparation process and improve the drug instructions can reduce the adverse reaction occurred to some extent of Chinese patent medicine.

Objective To investigate the effects of low dose radiation on dendritic growth of newborn neurons in the hippocampus of young rat.Methods One month-old male rats were randomized into radiation group aind sham control group.Radiation group received whole brain irradiation at a single dose of 2 Gy.Retrovirus expressing green fluorescent protein (GFP) was used to label newborn neurons in the hippocampus through stereotaxic intracranial infusion.Immunofluorescence assays were performed to detect dendritic architecture alterations induced by irradiation at different time points.Results Compared with control group,the lengths of total dendrite and the longest dendrite significantly decreased at 2 and 4 weeks after irradiation (t =3.10,2.07,2.94,4.02,P ＜ 0.05).The branching points of new born neurons were also decreased significantly at 2 weeks post irradiation (t =2.23,P ＜ 0.05).The number of new born neurons reduced at 4 weeks post irradiation (t =8.43,P ＜ 0.05).Conclusions Low dose radiation could inhibit newborn neuron growth in the hippocampus of young rat,which may be one of the most important mechanisms involved in radiation-induced cognitive impairment.

Objective To analyze the epidemiological characteristics of reported emergency public health events at schools from 2006 to 2013, and provide a scientific basis for better disposal of school-related events. Methods The reported emergency public health events at schools in Tianjin from 2006 to 2013 were collected, and descriptive epidemiological analysis was conducted on data. Results A total of 267 reported emergency public health events at school in Tianjin during 2006-2013, of which 257 infectious disease events, accounting for 96.25%. The incident occurred in primary schools and nurseries. The peak was from March to June and October to December for the incident. The top three species of disease onset were influenza (2 360 cases), chicken pox (2 032 cases) and mumps (1 813 cases). There was a correlation between the duration and interval from onset of the first case to report of emergency public health events(rs=0.522,P＜0.05). Conclusion In recent years, school public health emergency events are high momentum, mainly in rural areas, primary schools and nurseries. Timely detection and disposal of emergency public health events can reduce the harm generated by them.

Objective:To establish a method for the determination of tanshinoneⅡA and salvianolic acid B in Zhiyou capsules by HPLC. Methods:The chromatographic column was Waters Atlantis@T3(150 mm × 4. 6 mm,3 μm)using acetonitrile and aqueous ammonium acetate solution(adjusting pH to 2. 4 with formic acid)as the mobile phase with gradient elution,the flow rate was 1. 0 ml ·min-1 and the detection wavelength was 260 nm. Results:The contents of salvianolic acid B and tanshinoneⅡA showed good linear relationship within the range of 1. 20-60. 00μg·ml-1(r=0. 999 7)and 0. 40-20. 00 μg·ml-1(r=0. 999 8),respectively. The av-erage recovery was 101. 53%(RSD=1. 14%)and 95. 17%(RSD=4. 33%),respectively. Conclusion:The method is efficient,con-venient and accurate,which can be used in the quality control of Zhiyou capsules.