1.The Investigation on Treatment Status of Sewage Discharged From Hospitals in Jinan
Meili XIAO ; Xianna LIU ; Jun SUN ;
Journal of Environment and Health 1992;0(05):-
Objective To investigate the status of wastewater treatment of hospital sewage in Jinan. Methods The indices of COD, NH3,N and PO43- in sewage discharged from 16 hospitals before and after treatment and the content of residual chloride and fecal coliform counts in treated sewage were monitored in 2002 in Jinan. The monitoring results were evaluated according to the Criteria for Wastewater Discharge (GB/T8978-1996) and compared with the analytic results obtained in the year 2001. Results The COD, NH3,N and PO43- of the sewage of 16 hospitals before treatment were 253.8, 47.76 and 3.134 mg/L respectively with the over standard rate 81%, 88% and 100% respectively. The COD, NH3,N, PO43-, content of residual chloride and fecal coliform counts in treated sewage of 16 hospitals were 40.5, 6.30, 0.580, 26.7 mg/L and
2.Investigation on Occupational Hazards and Electric Welding Operation
Jinyan SUN ; Jing LIU ; Meili LI
Journal of Environment and Health 2007;0(09):-
Objective To study occupational hazards effects of electric welding,and evaluate protective measurements for different occupational hazards. Methods During 2008,occupational health services for 11 factories with shield carbon-dioxide arc welding work were provided,including surveillance of the work environment and surveillance of workers’health. Surveys of workers’health through various types of health examinations were carried out. Four hundred and thirty-three welding workers of 11 factories were included. Results The concentrations of 17 welding fume samples and 6 manganese dioxide samples were over the released limits of national standard,the maximum was 5.5,1.6 times of the limit respectively. The exceeding standard rate. of welding fume had positive correlation with the density of welding equipment(r= 0.72,P=0.01) and negative correlation with the ventilation area(r =-0.63,P =0.04) .The examination results showed that nobody suffered occupational diseases or occupational contraindications. Conclusion For the environmental risk factors existed in the electric welding operation site,more attention should be paid to the potentially occupational adverse effects of electric welding.
3.The dynamic change of galectin-1 in in mice with viral myocarditis
Jin SUN ; Bo HAN ; Yongfen CHEN ; Meili WEI
Journal of Clinical Pediatrics 2013;(10):949-952
Objectives To investigate the dynamic change of galectin-1 in myocardium of mice with viral myocarditis (VMC). Methods A total of 58 male BALB/c mice were selected and randomly divided into CVB3 group (n=50) and control group (n=8). Mice in CVB3 group were infected with 0.10 ml 10-5/L CVB3 through intraperitoneal inoculation, and 8 mice were killed on day 7, 10, 14 and 28 respectively after inoculation. Mice in control group inoculated with 0.10 ml eagle reagent was killed on day 28. The myocardial pathological changes were observed using light microscope. In addition, expressions of serum galectin-1 were detected by ELISA and expressions of myocardial galectin-1 were detected by real-time quantitative-polymerase chain reaction (RQ-PCR). Results Compared with control group, the myocardial expression of galectin-1 mRNA in CVB3 group was obviously increased on day 7 and day 10 (all P<0.05);the serum concentration of galectin-1 in CVB3 group was obviously increased on day 7, day 10 and day 14 (all P<0.05). Conclusion The expressions of galectin-1 in myocardium of mice with viral myocarditis were in dynamic changes. Galectin-1 may play a role in the pathogenesis and development of viral myocarditis.
4.Effect of cIAP1 down-regulation mediated by shRNA on biological behavior of human ovarian cancer cells
Hong JIN ; Kai XIE ; Meili ZHANG ; Jing SUN ; Youyuan DONG
Practical Oncology Journal 2015;(1):17-21
[Abstratc] Objective The function of cIAP1 in the progression of ovarian cancer has not been clarified . This study is to explore the involvement of cIAP 1 in regulating biological behaviors of ovarian cancer cells by u-sing RNA interference(RNAi)technology.Mte hods The short hairpin RNA plasmid targeting cIAP1 was con-structed and transfected into Skov 3 cells.The levels of cIAP1 mRNA and protein were investigated by RT -PCR and Western Blot respectively .MTT assay and flow cytometry were used to evaluate cell proliferation and apopto-sis.R esults The rate of cIAP1 transfection was 74.7%performed by flow cytometric analysis .cIAP1 expression was significantly down -regulated at both mRNA and protein levels ,which resulted in a decrease of cell prolifera-tion and invasion capability in vitro .Conclusion This study implies that cIAP 1 might play an important role in the progression of ovarian cancer ,and it could be a potential target for therapeutic anti -cancer drugs .
5.APPLICATION OF LAPAROSCOPIC ULTRASONOGRAPHY IN ADNEXAL OPERATIONS
Yunxiang NI ; Jing SUN ; Xiaofei SHI ; Meili LIN ; Fang YIN ;
China Journal of Endoscopy 2002;8(2):4-6
Objective:To explore the clinical value on application of laparoscopic ultrasonography (Lap US) in the adnexal operation. Methods:Eleven patients including 7 cases of tubal pregnancy, 3 cases of teratoma of ovary and 1 case of endometrial cyst of ovary were examined by LapUS, then operated with laparoscopy.Results:The results showed that the modality presented is a big progress over the traditional operative management for adnexal diseas. Conclusions:It is a good approach for micro-surgery in the adnexal operation by laparoscopy.
6.GC-MS analysis of ether extracts from three species of Chaenomeles fruits
Yongfu HONG ; Lianna SUN ; Xuemin GUO ; Meili GUO ; Yiming LI ;
Academic Journal of Second Military Medical University 2000;0(08):-
Objective:To analyze the chemical constituents of the ether extracts from the fruits of 3 species of Chaenomeles . Methods: The chemical constituents were analyzed by GC MS. Results: Sixty one constituents were firstly isolated and identified from the fruits of 3 species of Chaenomeles . Forty compounds in Chaenomeles lagenaria , thirty four compounds in Xuan mugua ( C.lagenaria from Xuanzhou) and 23 compounds in C.sinensis were isolated and identified respectively. There were 10 compounds in all 3 species of Chaenomeles , but their contents were very much different. They were acetic acid, benzaldehyde, n caproic acid, 14 methyl pentadecanoic methyl ester, glycerin, benzoic acid, 6,9 octadecadienoic methyl ester, squalane, stearic acid and oleic eicosyl ester. Conclusion: The composition and content vary with different species and district of Chaenomeles . The result may be useful for the evaluation of Chaenomeles fruits quality. [
7.Imaging diagnosis of chondromyxoid fibroma
Jinghong YU ; Meili TAO ; Yunfeng ZHANG ; Wei LI ; Ruifen SUN
Chinese Journal of Postgraduates of Medicine 2015;38(2):105-108
Objective To analyze the imaging manifestations of chondromyxoid fibroma,so as to improve diagnostic accuracy.Methods The X-ray,CT and MRI manifestations of 8 cases with chondromyxoid fibroma confirmed by surgical pathology were analyzed retrospectively.Results All of 8 cases were in the long tubular bones.X-ray showed oval,well defined,eccentric,radiolucent lesion with surrounding sclerosis.The thick dense bony or coarse reticular septation were seen in 6 cases.CT scan showed osteolytic,surrounding sclerosis,and 1 case with foci of calcification.MRI images showed a low or medium signal on T1WI,high signal on T2WI,1 case with a equal T1WI and slight high T2WI signal intensity peripheral rim around.Two cases with diffused enhancement on T1WI after intravenous injection of gapentetate acid meglumine injection and a peripheral liner enhancement in 1 case.Conclusion Chondromyxoid fibroma has certain imaging characteristics,but should be combined with X-ray,CT and MRI to differentiate from other bone tumors with similar imaging manifestations.
8.Exploring in management of women with atypical squamous cells of undetermined significance
Lingling SUN ; Meili WANG ; Huiqin WANG ; Lina WANG
The Journal of Practical Medicine 2014;(20):3296-3298
Objective Exploring in processing method to triage of women with a cytologic smear showing atypical squamous cells of undetermined significance. Methods All 130 cases were diagnosed with ASCUS received high-risk HPV (hr-HPV)test, colposcopic inspections, reformeded Reid's colposcopic index score and cervical biopsies for pathological examination. Resluts The pathology of 130 ASCUS patients included 31cases of normal and inflammation (23.90%), 29 cases of CINⅠ(22.30%), 14 cases of CINⅡ(10.80%), 55 cases of CINⅢ or cervical carcinoma in situ (42.30%), and 1 case of invasive cervical carcinoma (0.80%). The reformed Reid's colposcopic index score of 130 ASCUS patients included 32 cases of 0 score (24.60%), 36 cases of 1~2 scores (27.70%), 47 cases of 3~4 scores(36.10%), 15 cases of 5~6 scores(11.60%). 2. The colposcopy used to detect the CIN of ASCUS, its sensitivity was 98.99%(98/99), specificity was 51.61%(16/31). The hr-HPV used to detect the CIN of ASCUS, its sensitivity was 96.97%(96/99), specificity was 32.26%(10/31). Conclusion Both the reformeded Reid′s colposcopic index score and hr-HPV testing as the clinical triage methods of ASCUS. The former has good sensitivity and specificity ,which is superior to hr-HPV testing.
9.Highly Fluorescent Carbon Nanoparticles for Sensitive Detection of IronⅢ
Pingping YIN ; Kelong AI ; Meili LI ; Guoying SUN
Chinese Journal of Analytical Chemistry 2014;(10):1427-1433
A simple preparative strategy was proposed for the preparation of water-soluble, bright blue-emitting carbon nanoparticles ( CNPs ) at different temperatures by hydrothermal treatment using bean curd residue as carbon source for the first time. When the temperature reaches 240 ℃, quantum yield ( QY) can be up to 15. 24%. Most importantly, such fluorescent CNPs can be served as an effective sensing platform for label-free sensitive and selective detection of Fe3+ions with a detection of limit as low as 50 nmol/L, which is nearly 2 orders of magnitude lower than the maximum level of Fe3+ions in the drinking water permitted by the European Community. These outstanding properties give promising potential for reliable detection of Fe3+ in intracellular fluid and water samples, even bioimaging and optical imaging without being hazard in the future.
10.Pathophysiological role of angiotensin converting enzyme 2
Jing HONG ; Ming NIE ; Meili SUN ; Dawang WANG
Basic & Clinical Medicine 2006;0(09):-
The rennin angiotensin system has been recognized for many years as a key regulator in systemic blood pressure and the metabolism of water and salt. Angiotensin converting enzyme 2(ACE2) is the first human homolog of angiotensin converting enzyme which efficiently hydrolyze the angiotensin Ⅱ to vasodilator angiotensin 1-7.ACE2 is now implicated in cardiovascular,renal, lung disease and serves as a receptor for Severe Acute Respiratory Syndrome coronavirus.