By observing the changes in LDH、AST、CHE、K、Na+ contents in supernatant of cultured rat pituitary cells after electromagnetic pulse (EMP) irradiation the injury mechanism to pituitary cells was explored. Pituitary cells of Wistar rat was cultured in 6-hole plates. The cells in five plates were irradiated with high field strength EMP ( electromagnetic pulse) for 5 times within 2 minutes. The electromagnetic pulse simulator provided a high electric field intensity of 60 KV/m, with rise time 20-nsec and pulse wide 30/?S. Contents of LDH, AST, CHE, K+ and Na+ in the supernatant were measured using reagent boxes (Beijing Zhongsheng High-tech Bioengineering Company) at Oh, 1h, 6h, 12h and 24h following irradiation, respeetively. All data were analyzed by statistical software Spss 8. 0. The results showed that LDH and CHE contents in the supernatant increased significantly at Oh after irradiation with EMP (P 0. 05). Our conclusion is EMP could injure membranes of pituitary cells.

By observing apoptosis and morphological changes in primary culture of neurons from cerebellar granular layer, the neurons were irradiated with EMP (electromagnetic pulses), the possible injury mechanism was explored. The changes in death and apoptosis of the neurons were detected by MTT and flow cytometry, respectively. The slides stained with HE and TUNNEL were examined with light and fluorescence microscopy. The results showed that not only death of the neurons occurred immediately, but also apoptosis appeared after irradiation with EMP. Our conclusion is EMP can promote necrosis and apoptosis of neurons from cerebellar granular layer at early stage, which may result from DNA injury induced by EMP.

Objective To assess the value of ultrasound in evaluating clinical effect of treatment of hysteromyoma uterine by selective uterine artery embolization ( UAE ) .Methods 42 hysteromyoma uterine in 30 cases were examed by color doppler ultrasound befort and after uterine artery embolization with one year monthly.The size , morphology,internal echotexture and doppler signal of the uterus and myoma were observed , and the doppler signal of uterine artery , peripheral and interal vascular in myoma were measured . Results There was significant difference of the average colume of the uterus and its myoma between preoperation and postoperation ( 491.37 cm3 vs 236.75 cm3,102.33 cm3 vs 48.87 cm3 respectively,?

目的探讨药物注射配合强化训练对颅脑损伤患者运动功能的影响。方法将60例住院颅脑损伤患者随机分为治疗组(30例)和对照组(30例)。治疗组在常规综合康复治疗基础上加用针对性药物注射治疗及强化训练,而对照组单纯采用康复治疗。两个疗程(20天/疗程)后,采用Fugl- Meyer运动功能量表评定两组的治疗效果。结果两组患者的运动功能均有改善 ,但治疗组的改善程度明显优于对照组(P<0.01)。结论药物注射配合强化训练能够明显改善颅脑损伤患者的运动功能。

Objective To analyze the consistency of transcutaneous perianal ultrasonography (TPUS) and pelvic magnetic resonance imaging (MRI) in diagnosing perianal lesions of Crohn's disease (CD), and to evaluate the value of transcutaneous perianal ultrasonography in detecting perianal lesions of CD. Methods A cohort of 102 patients diagnosed as Corhn's disease were enrolled from August 2008 to August 2010. Perianal abscess and fistula of these CD patients was diagnosed by ultrasonography and MRI system. Statistics was performed with SPSS 11.5 software for X2 test. The consistency was analyzed with Kappa test. Results The mean onset time of perianal lesions in CD was -0.443 year (95％CI:-1.659～0.773 year) before typical symptoms showed up. There was no significant difference in detecting perianal lesions of CD between transcutaneous perianal ultrasonography and pelvic magnetic resonance imaging (P ＝ 0.706, Kappa ＝ 0.541). If pelvic magnetic resonance imaging was considered as the golden standard in detecting perianal lesions of CD,the sensitivity (Sen), specificity (Spe), Youden's index, positive predictive value (PPV) and negative predictive value (NPV) of TPUS were 72.73％, 82.61％, 0.55, 66.67％ and 86.36％ respectively.Furthermore, there was no significant difference between transcutaneous perianal ultrasonography and pelvic magnetic resonance imaging in detecting perianal abscess ( P ＝ 0.706, Kappa ＝ 0.496) and fistula (P＝0.655, Kappa＝0.546) of CD. Conclusions Perianal lesions occur in the entire course of CD. There was favorable consistency between transcutaneous perianal ultrasonography and pelvic magnetic resonance imaging in detecting perianal abscess and fistula of CD. Transcutaneous perianal ultrasonography can be used as an additional method in detecting and evaluting perianal lesions of CD.

Objective To investigate the protective effect of somatostatin (SS)combined with growth hormone (GH) in treatment of intestinal mucosal barrier injury in rabbits with severe acute pancreatitis (SAP), as well as its clinical significance. Methods Seventy-two rabbits were equally assigned into model group (SAP group), SS treated group (SS group) and SS combined with GH treated group (SS + GH group). SAP models were induced by retro-injection of 5% sodium taurocholate into the pancreatic duct. After modeling, all rabbits were given 5 % glucose saline daily.The rabbits in SS group and SS+GH group were continuously Given SS (3.5μg·kg-1·h-1)for 48 hours. Besides, the rabbits in SS+GH group were subcutaneously injected with 0.15 IU/kg of GH at the 1st and the 24th hours after modeling. The levels of serum amylase, serum tumor necrosis factor-α (TNF-α) and plasma diamine oxidase were measured at the 6th, 12th, 24th and 48th hours after modeling. The pathological changes of pancreatic tissue and ileal mucosa were observed. Survival rate was calculated. Data were analyzed using SPSS 16.0 software. The univariate analysis was used to compare the difference among groups. Results In SS+GH group, the levels of serum TNF-α and plasma diamine oxidase were (2. 43 ± 0. 14) pg/ml and (4. 61 ± 0. 45) U/L at the 24th hour respectively, and were (2.08±0.23) pg/ml and (3.75±0.47) U/L at the 48th hour, respectively,which were lower than those in SAP group and SS group [(2.80 0.30) pg/ml and (8.74 ± 1.77)U/L, respectively, at the 24th hour; (2. 45±0.12) pg/ml and (5. 02±0.95) U/L, respectively, at the 48th hour)]with significant difference (P＜0.05). The inflammation in pancreas and ileal mucosa was alleviated, and the integrity of bowel mucosa was improved. Survival rate of SS+GH group was significantly higher than SAP group and SS group. There was no significant difference in level of serum amylase between SS+GH group and SS group. Conclusion The combination of SS with GH may enhance the function of intestinal mucosa barrier and improve the prognosis of SAP in rabbits.

Objective To study the feasibility and security about misoprostol combined with fentanyl and propofol applied in visualization of artificial abortion.Methods 1270 cases of pregnant women who requested termination of pregnancy and voluntarily accepted abortion were selected.In the observe group,634 cases of pregnant women received oral misoprostol about 600 mg 90 minutes before surgery.After anesthetized by fentanyl and propofol,painless artificial abortion using visual abortion instrument was carried out.In the control group,636 cases of pregnant women anesthetized by fentanyl and propofol via the venous were carried out artificial abortion.The degree of cervical relaxation,loss of blood,total dose of propofol,surgical time,recovery time,the incidence of abortion complications and so on were observed.Results In the observation group and the control group,the average dosage of propofol are( 14.15 ± 2.37) ml and (16.90 ± 4.52) ml respectively;The average time required for surgery are ( 146.1 ± 38.2) s and ( 199.7 ± 37.3 ) s respectively;the average time required for awakening are ( 6.01 ± 1.90 ) min and ( 8.81 ± 2.02 )in;In the observation group,there are 405 ( 63.9% ) cases of cervical laxity markedly,in the control group,including 145 ( 22.8% ) cases of cervical laxity markedly,there is statistical significance ( P ＜ 0.05 ) between the observation group and the control group.There are no abortion syndrome in both groups.Conclusion Misoprostol combined with fentanyl and propofol was the first choice for painless artificial abortion using ultrasound visualization.

Objective To evaluate normal maturation of the fetal brain with half-Fourier rapid acquisition with relaxation enhancement(RARE) MRI.Methods The normal brains of 25 fetuses of 12-38 weeks gestational age were examined in utero with half-Fourier RARE imaging.Gyrus maturation,gray and white matter differentiation,ventricle-to-brain diameter ratio,and subarachnoid space size were evaluated with respect to gestational age.Results At 12-23 weeks,the brain had a smooth surface,and two or three layers were differentiated in the cerebral cortex.At 24-26 weeks,only a few shallow grooves were seen in the central sulcus,and three layers,including the immature cortex,intermediate zone,and germinal matrix,were differentiated in all fetuses.At 27-29 weeks,sulcus formation was observed in various regions of the brain parenchyma,and the germinal matrix became invisible.Sulcation was seen in the whole cerebral cortex from 30 weeks on.However,the cortex did not undergo infolding,and opercular formation was not seen before 33 weeks.At 23 weeks and earlier,the cerebral ventricles were large;thereafter,they gradually became smaller.The subarachnoid space overlying the cortical convexities was slightly dilated at all gestational ages,most markedly at 21-26 weeks.Conclusion Changes in brain maturation proceed through stages in an orderly and predictable fashion and can be evaluated reliably with half-Fourier RARE MRI.

Objective To explore the value of application of choledochofiberscopy in the diagnosis and treatment of the extrahepatic bile duct disease, and the effect on reducing the incidence of the postoperative residual stone in biliary ducts. Methods According to the case history and ultrasonography,if the common bile duct(CBD) diseases suspected,the CBD was explored by intraoperative choledochofiberscope(IOCF). During the procedure,a biliary passage mirror inducer apparatus and biliary tract probe which were manufactured by ourselves were used. Results During LC,IOCF was performed on 385 cases of the 10 396 LC cases,and possitive findings were dicovered in 102 cases(26.49%). Among those positive patients, 67 cases belonged to stricture of the lower biliary tract; 5 cases were Mirizzi syndrome; 2 cases were carcinoma of the periampulla; 1 case was primarily carcinoma of the bile duct; 1case was ascarisis of the biliary system. Conclusions IOCF is a good inspect technique with high success rate and clear image of bile duct, it can discover the common duct diseases which are difficult to be diagnosed through the routine examination.At the same time, it can provide the locative and qualitive diagnosis, determine reasonable methods of operation,and effectively provent postoperative complications.

The pathological changes of pulmonary fat emdoiksm were studied in ratsby means of an intravenous injection with autosnbcutaneous fat.The resultsshowed that most fat ernboli appeared in pulmonary arterioles and capillariesafter 24 hours.By 4 days,large number of sudan Ⅲ-positive emboli were seenthroughout the lungs.The inflammatory cellular infiltration including the histoc-ytes,macrophages,polymorphonuclear leucocytes and occasional eosinophils,pulmonary edema and focal hemorrhage were observed.After 7 days,most fatemboli disappeared.No fat embolism in other organs was found.