Objective To study the expression of Gal?-1,4-GlcNAc group in human normal brains and astrocytomas. Methods The protein extracts from human normal brains and astrocytomas were subjected to sodium dodecyl sulfate polyacrylamide gel electropheresis(SDS-PAGE),then were stained with Coomassie Brilliant blue(CBB).In addition,the expression of Gal?-1,4-GlcNAc group was studied by ricinus communis agglutinin-I(RCA-I) lectin blot and lectin histochemistry with RCA-I respectively. Results By comparison,CBB staining showed that the protein components from normal brains were similar to those from astrocytomas.However,by RCA-I lectin blot and histochemistry assay,it was found that the expression of Gal?-1,4-GlcNAc was more extensive in astrocytomas than normal brains.It was surprising that a major 61?kD protein was galactosylated in astrocytomas but not in normal brains.Ratio of RCA-I stained cells was 8% in human normal brains,while 15%,21%,28% and 41% in astrocytomas Ⅰ,Ⅱ,Ⅲ and Ⅳ respectively.Conclusion The expressions of Gal?-1,4-GlcNAc groups in astrocytomas were more than those in normal brains,which suggested that Gal?-1,4-GlcNAc group might be related to the malignant degree of astrocytomas.

Objective To investigate the protective effect of Xinan granule on viral myocarditis in mice. Methods Viral myocarditis model was made by intraperitoneal inoculation with Coxsackie virus B3 in BALB/C mice. Mice were randomly devided into six groups:the normal control group, the model control group, the Xinan granule10、20、40 g/(kg?d) groups and the Binduzuo 0.1 g/(kg?d) group.After CVB3 infection 2 h, one set of mice were given drugs for 7 d.Blood and heart samples were obtained on the 8th day after CVB3 infection.The myocardial histopathologic changes were observed. The serum cardiac enzyme (LDH) were measured.The others were given drugs for 2 d before CVB3 infection, and for 7ds after CVB3 infection. The survival time were recorded for 20 ds. Results The myocardial histopathologic damage, LDH, cardiac index, punctate hemorrhage on heart surface in Xinan group reduced more significantly than that of the model control group. The survival time in Xinan group were longer than that of the model control group. Conclusion Xinan granule may have the preventional and therapeutic effect on viral myocarditis in mice.

Objective To investigate the effect of curcumin on the expression of nuclear transcription factor (NF-kappa B) and its inhibitory protein (Iκ-Bα) in liver cancer cells (Hepg-2) induced by TNF-α, and explore its effect on non-alcoholic steatohepatitis. Methods The cells were divided into normal group, TNF-alpha group, and different concentrations of curcumin treatment group. MTT and Western blotting were used to assay curcumin’s effect on Hepg-2 proliferation activity and changes of NF-κB and Iκ-Bα’s in Hepg-2. Results Compared with the normal group, TNF-αgroup enhanced NF-κB and Iκ-Bαexpression, but the increase of Iκ-Bα had no statistical significance (P>0.05). Curcumin treatment group’s NF-κB expression was significantly weakened than TNF-α group, while Iκ-Bα was significantly enhanced than that of TNF-α group. Conclusion Curcumin can antagonize NF-κB inflammatory signaling pathway activation induced by TNF-α, thus reduce the inflammatory injury of the liver cells.

Objective To explore the treatment for patients with advanced gastric cancer, the use of xiaoaiping injection combined with FOLFOX6 chemotherapy intervention. Methods 40 cases of patients with advanced gastric cancer for the implementation of FOLFOX6 chemotherapy intervention, at the same time as the control group, the other 40 patients in the control group based on the use of xiaoaiping injection, and classified as the observation group, two groups of patients were in our hospital from March 2015 to January 2017 . Results Comparing the two groups of patients with the treatment efficiency, the patients in the observation group were higher obviously; the observation group patients nursing satisfaction rate was significantly higher (P<0.05) shows significant differences. Compared two groups of patients with adverse reactions, found in the two groups were different, but the difference was not obvious. Conclusion The visible analysis of the effect of xiaoaiping injection combined with FOLFOX6 chemotherapy in the treatment of advanced gastric cancer, compared with chemotherapy and intervention The utility model has the advantages that the patient can be improved in time, and the clinical treatment effect can be improved, so that the utility model is worthy of clinical reference.

Objective: To study the effect of HaikunShenxi on expression of connective tissue growth factor(CTGF) in renal tubules of STZ-induced diabetic nephropathy rats.Methods: SD rats were randomly divided into two groups: normal control and diabetic nephropathy.Diabetes was induced by intraperitoneal injection of STZ.The diabetic models were affirmed upon blood glucose ≥16.5 mmol/L,urinary glucose ≥ ++++ 72h after the injection.And the diabetic rats were randomly divided into four groups: model group,irbesartan treatment group,low and high dose HaikunShenxi treatment group.24-hour urine volume,24-hour urinary protein excretion were examined at week 4,8,and 12 respectively.All the rats were sacrificed at week 12;body weight,kidney weight,and serum excretion,serum creatinine,blood urea nitroge were detected.Kidney were taken for pathological examination(HE,Mallory stain),The expressions of CTGF in rat renal were detected respectively by immunohistochemistry and in situ hybridization.Result: 24-hour urine volume,24-hour urinary protein excretion were examined at week 4,8,and 12 respectively.All the rats were sacrificed at week 12;body weight,kidney weight,and serum excretion,serum creatinine,blood urea nitroge was improved by HaikunShenxi preparation.Haikunshenxi can alleviate severe pathological changes of kidney structure in rats with DN,The expression of CTGF in the HaikunShenxi treatment group was depressed greatly.Conclusions: The overexpression of CTGF in renal tissue of diabetic rats may be one of the mechanisms of diabetic nephropathy,haikunshenxi had certainly protective effects on it.

Objective To study the expression of tansforming growth factor(TGF)-?1 and mRNA in the renal tubules of Streptozotocin(STZ)-induced diabetic nephropathy rats.Methods SD rats were randomly divided into two groups: normal control and diabetic nephropathy.Diabetes was induced by intraperitoneal injection of STZ.And the diabetic rats were randomly divided into four groups: model group,irbesartan treatment group,Haikunshenxi low and high dose group.Twenty-four-hour urine volume and urinary protein excretion were examined at week 4,8,and 12 respectively.All the rats were sacrificed at week 12,and body weight,kidney weight,and serum excretion,serum creatinine,blood urea nitrogen were examined.The expression of TGF-?1and mRNA in rat renal tissue were detected respectively by immunohistochemistry and in situ hybridization.The graphic analysis system and SPSS 11.5 statistics software were used to treat the results.Results The expression of TGF-?1and mRNA in the Haikunshenxi group was depressed greatly(P

For unresectable advanced hepatocellular carcinoma (HCC),besides sorafenib,alternative drugs and treatment modalities are required.Clinical studies of hepatic arterial infusion chemotherapy (HAIC),transcatheter arterial chemoembolization (TACE),and system chemotherapy have shown favorable efficacy and tolerance in advanced HCC patients.In addition,the potential efficacy of sorafenib combined with focal treatment is also an interesting issue.As more therapies become available,decision-making for treating advanced HCC becomes increasingly complex.In our opinion,diverse treatment modalities should be utilized for the best interest of patients.Based on predictive biomarkers,we should develop a precise patient stratification system to select suitable candidates for each treatment modality in future studies,as is useful for improving prognosis of patients with advanced HCC.

Bacillus amyloliquefaciens B10-127 was used to produce 2,3-butanediol (2,3-BD) from residual glycerol obtained from biodiesel synthesis. Important variables for 2,3-BD fermentation, pH and dissolved oxygen, were studied. When pH was maintained constant, the yield of 2,3-BD was inhibited. The highest 2,3-BD yields were achieved by fermentation without any pH control with an optimized initial pH 6.5. Batch fermentative production of 2,3-BD by B. amyloliquefaciens was investigated using various oxygen supply methods by changing agitation speed. Based on the analysis of three kinetic parameters including specific cell growth rate (micro), specific glucose consumption rate (q(s)) and specific 2,3-BD formation rate (q(p)), a three-stage agitation speed control strategy was proposed, aimed at achieving high concentration, high yield and high productivity of 2,3-BD. Maximum concentration of 2,3-BD reached 38.1 g/L, with the productivity of 1.06 g/(L x h), which were 14.8% and 63.1% over the best results from constant agitation speeds. In a pulse fed-batch fermentation, 2,3-BD concentration and productivity were significantly improved to 71.2 g/L and 0.99 g/(L x h), respectively. To our knowledge, these results were the highest for 2,3-BD production from biodiesel-derived glycerol.
Bacillus ; classification ; metabolism ; Biofuels ; analysis ; Bioreactors ; Butylene Glycols ; metabolism ; Fermentation ; Glycerol ; metabolism ; Hydrogen-Ion Concentration ; Industrial Microbiology ; Oxygen ; analysis

Objective To investigate the effect of gender factors on the reduction of ischemia-reperfusion (I/R) injury by sevoflurane postconditioning in isolated rat cardiomyocytes. Methods Sixty 2-month-old SD rats (30 male, 30 female) were used in this study. Male rats were randomly assigned into 2 groups (n = 15 each):control group (group MC) and sevoflurane postconditioning group (group MS). Female rats were also randomly assigned into 2 groups (n= 15 each): control group (group FC) and sevoflurane postconditioning group (group FS).The rats were anesthetized with intraperitoneal pentobarbital 60 mg/kg. Their hearts were excised and perfused in a Langendorff apparatus with K-H solution saturated with 95% O2-5% CO2 at 37 ℃. I/R was produced by 40 min of global ischemia followed by 120 min of reperfusion. Control groups received perfusion with K-H solution saturated with O2 . Sevofiurane postconditioning groups received 10 min of perfusion with K-H solution saturated with 3%sevoflurane and O2 and then 110 min of perfusion with K-H solution saturated with O2 . HR, left ventricular enddiastolic pressure (LVEDP) and left ventricular developed pressure (LVDP) were measured before ischemia and during reperfusion. Coronary effluent was collected at 5 min of reperfusion for determination of LDH activity and infarct size. The total Akt (t-Akt) and phosphorylated Akt (p-Akt) expression in cardiomyocytes was detected. The ratio of p-Akt to t-Akt (p-Akt/t-Akt) was calculated. Results LVDP, p-Akt expression and p-Akt/t-Akt were significantly higher, LVEDP and LDH activity were significantly lower, and the infarct size was smaller in group MS and FC than in group MC (P ＜ 0.05). LVDP was significantly lower, LVEDP and LDH activity were significantly higher, and the infarct size was larger in group FS than in group MS. There was no significant difference in LVDP and LVEDP between group FC and FS (P ＞ 0.05). Conclusion There are gender differences in the reduction of I/R injury by sevoflurane postconditioning in isolated rat cardiomyocyes, the protective effect is stronger in male rats than in female rats, and the differences may be related to the activation of Akt.

Objective To evaluate the effects of the right stellate ganglion block on the expression of β3adrenoceptor (β3-AR) in rabbits with heart failure.Methods Forty-eight Japanese white rabbits of both sexes,weighing 2.5-3.0 kg,were randomly divided into 3 groups (n =16 each):sham operation group (group S),heart failure group (group HF) and right stellate ganglion block group (group RSGB).Heart failure was induced by occlusion of left anterior descending branch of coronary artery and confirmed by ultrasonic cardiography 4 weeks later.A PE-10 catheter was inserted into the right stellate ganglion for administration of drugs.0.25％ bupivacaine 2 ml was injected through the catheter once a day for 2 weeks in group RSGB,while the equal volume of normal saline was injected instead of bupivacaine in S and HF groups.The left ventricular end-diastolic diameter (LVEDD),left ventricular end-systolic diameter (LVESD),ejection fraction (EF) and left ventricular fractional shortening (LVFS) were measured at 1 day before ligation (T0),before catheter insertion (T1),before 8th administration (T2),and 1 day after the last administration (T3).Eight rabbits were sacrificed at T1 and T3 in each group and myocardial specimens were obtained from the apex of the left ventricle for determination of the expression of β3-AR by Western blot.Results Compared with group S,the LVEDD and LVESD were significantly enlarged and LVEF and LVFS were decreased at T1-3,and the expression of β3-AR was up-regulated at T1,3 in groups HF and RSGB (P ＜ 0.05).Compared with group HF,the LVEDD and LVESD were significantly decreased,LVEF and LVFS were increased,and the expression of β3-AR was significantly down-regulated at T3 in group RSGB (P ＜ 0.05).Conclusion The right stellate ganglion block can improve the cardiac function of rabbits with heart failure through down-regulating the expression of β3-AR in myocardium.