1.The survey about health knowledge condition in HBV-positive pregnant women
Meijuan BAO ; Yan LU ; Xiaoling WANG ; Juan ZHANG ; Lin ZHANG
Chinese Journal of Practical Nursing 2009;25(23):34-36
Objective To investigate the HBV- positive pregnant women about the knowledge of the route of hepatitis B transmission, hepatitis B hazards, health care during pregnancy, announcements of child- care to explore targeted and effective health education model with self- designed question-naires. Methods Nurses in obstetrics department carried out this questionnaire investigation in the outpa-tients of HBV- positive pregnant women, after that the results underwent statistic analysis. Results The overall knowledge of HBV- positive pregnant women in announcements of child- care, health care during pregnancy and hepatitis B hazards was not very well, and the announcements of child- care was the lowest, followed by health care during pregnancy and hepatitis B hazards. The route of hepatitis B transmission was better, but still not satisfactory. The education should highlight these areas. Well- educated women were better than those who lack education. The age between 20 to 30 years old was better. Conclusions The HBV-positive pregnant women is lack of heath knowledge, so we should gave health intervention in a multi- level, multi- stage standardized and a variety of ways.
2. A path analysis of impacts of social support and rumination on posttraumatic growth of patients with human immunodeficiency virus
Shenmin WAN ; Hongzhou LU ; Meijuan BAO ; Lin ZHANG ; Meiyan SUN
Chinese Journal of Practical Nursing 2019;35(16):1207-1214
Objective:
To explore the effect of social support and rumination on posttraumatic growth of patients with human immunodeficiency virus (HIV).
Methods:
A total of 1 152 patients with HIV from Shanghai Public Clinical Center were investigated using General questionnaire, Perceived Social Support Scale, Event Related Rumination Inventory and Posttraumatic Growth Inventory by cross-sectional survey method from January 2018 to October 2018. The path of social support and rumination on post-traumatic growth was established by correlation analysis and structural equation model.
Results:
The total score of posttraumatic growth in patients with HIV was (47.93±23.55) points, which was at the low-middle level. Correlation analysis showed that posttraumatic growth was positively correlated with comprehension of social support (
3.Development of clinical practice guidelines for the management of adherence to highly active antiretroviral therapy
Liang FU ; Yan HU ; Hongzhou LU ; Meijuan BAO ; Lin ZHANG ; Yinzhong SHEN ; Lijun ZHA ; Cheng′en PAN ; Huiwen LI ; Zheng ZHU ; Ning DONG ; Yanjuan GAN
Chinese Journal of Practical Nursing 2016;32(19):1497-1501
Objective To develop the clinical practice guidelines for the management of medication adherence to highly active antiretroviral therapy (HAART) in China. Methods The development methods included qualitative interview of 31 stakeholders, questionnaire survey of 423 PLHIV, adaptation of 30 clinical practice guidelines related to AIDS care, and overviews of reviews of 44 systematic reviews/Meta-analysis. Results 10 clinical practice guidelines and 10 systematic reviews/Meta-analysis were included. The clinical practice guidelines for the management of HAART were formed. Conclusions The formed clinical practice guidelines showed better applicability and higher general quality. It is recommended to use the guidelines in AIDS care.
4.Chromomycin A(2) induces apoptosis of HepG2 cells in vitro.
Yan WANG ; Yuanyuan LU ; Weiwei BAO ; Meijuan XIE ; Zhenning DU
Journal of Southern Medical University 2014;34(10):1449-1453
OBJECTIVETo study the effect of chromomycin A(2) in inducing apoptosis of HepG2 cells and explore the molecular mechanism.
METHODSHepG2, MCF-7, A549, and 7901 cells were exposed to chromomycin A(2) and the changes in the cell viability were detected using MTT assay. The changes in the chromatins were observed with laser scanning confocal microscope after incubation of the cells with chromomycin A(2) (60 nmol/L) for 24 h. The changes in cell morphology were examined with a phase-contrast microscope, and the apoptotic cell populations, fluorescent intensity of reactive oxygen species (ROS) and mitochondrial membrane potential were determined using flow cytometry.
RESULTSChromomycin A(2) significantly inhibited the proliferation of the cells in a time- and dose-dependent manner, and caused changes in the cell morphology and cell apoptosis. Exposure of the cells to chromomycin A(2) resulted in chromatin condensation, ROS generation, and reduction of the mitochondrial membrane potential.
CONCLUSIONIncreased ROS and mitochondria damage may importantly contribute to chromomycin A(2)-induced apoptosis in HepG2 cells.
Apoptosis ; drug effects ; Cell Survival ; Hep G2 Cells ; drug effects ; Humans ; Membrane Potential, Mitochondrial ; Mitochondria ; pathology ; Plicamycin ; analogs & derivatives ; pharmacology ; Reactive Oxygen Species ; metabolism