Objective To compare the allodynic effect of intrathecal nociceptin between Swiss albino and C57 BL mice. Methods The allodynic effect was evaluated by allodynic scores by light touching using paintbrush. The allodynic effect of intrathecal nociceptin was observed over 50 min after injection in two mouse strains in a double blind manner. Results The allodynia induced by nociceptin lasted for 50 min in Swiss albino and no dose-response relationship was observed from dose 5 fmol- 50 pmol per mouse. In C57 BL mice, allodynic effect lasted for 30 min and showed a bell-shaped dose-response curve from dose 10 frnol-1 nmol per mouse. Conclusion These results demonstrate that the allodynic eflect of intrathecal nociceptin is different between strains.

The purpose of this study was prospectively to research the relationship between EEG power spectrum and anesthesia depth. Twenty-four patients,aged 20 to 50 years , ASA grade ,scheduled for selected surgical operations, were randomly divided into two groups:enflurane and isoflurane groups. Anesthesia was induced by thiopental 5 mg/kg and atracurium 6-7 mg/kg intravenously and maintained by inhalation anesthetics. After intubation patients were ventilated mechanically to maintain the level of PETCO2 between 4. 27 and 4. 93 kpa. NIBP,HR,SpO2 , ECG and EEG were recorded during anesthesia. According to TOF stimulation test intravenous atracurium 10-15 mg was given intermittently to maintain the ratio of T4/T1 less than 25%. Two channels EEG monitor was applied with the filtration range from 0 to 30 Hz Anesthesia level, adjusted at 0. 5,0. 8, 1. 0, 1. 3, 1. 5, 1. 3, 1. 0, 0.8 or 0. 5 MAC in sequence ,was maintained for 10 to 15 minutes before SEF (spectral edge frequency)and MPF (median power frequency) were recorded. NIBP decreased and HR increased with the inhaled agent concentration (IAC) level rising. The lowest BPs were 13. 1 /9. 33 kPa and 12. 9/8. 1kpa in both groups. There were negative remarkable correlations between either SEF or MPF and IAC in both groups (=- 0. 94 and -0. 82,P

The effects of different concentrations of enflurane and enflurane/nitrous oxide on EEG in 20 patients (ASA grade Ⅰ) were studied during elective surgery. They were divided randomly into two groups:enflurane (E) group (n=10) and enflurane/nitrous oxide (EN) group (n= 10). Anesthesia was induced by inhaling enflurane or enflurane/60% nitrous oxide. After intubation patients were venti lated mechanically to maintain P_(ET)CO_2 4.27—4.93 kPa, Two-leads EEG monitor was used during operation. 95% spectral edge frequency (SEF),MAP and HR were recorded in group E at 0.5,0.8,1.0,1.3 and 1.5 MAC, and in group EN at 0.8,1, 0,1.3,1.5,1.8 and 2.0 MAC. SEF decreased in linear manner,but MAP insignificantly changed as enflurane level increased. SEF was smaller in group E than that in group EN under the same additive MAC value, In raw EEG,patients in group E had spike wave at more than Ⅰ.0 MAC,but there was no spike wave in group EN. So enflurane/nitrous oxide anesthesia is more proper because nitrous oxide diminishs the undesirable effects of enflurane on brain during deeper anesthesia. The simple additive MAC value may not reflect the exact anesthesia level when nitrous oxide is used in combination, but SRF can do it.