Objective To analyze the effect of Huashi Xingyu Qingre decoction therapy through identification of the differentially expressed saliva proteins of oral lichen planus. Method The saliva of OLP patients before and after treatment were collected. Total saliva proteins were extracted. The differentially expressed saliva proteins were screened by two-dimensional fluorescence difference gel electrophoresis and identified by liquid chromatography-mass spectrometry. The differentially expressed proteins were analyzed by Western-blot. Results Six differentially expressed proteins were identified as salivary amylase, serum albumin, IgM, carbonic anhydrase VI, zinc-α2- glycoprote and sIgA. The expression level of serum albumin, IgM, carbonic anhydrase VI and zinc-α2-glycoprotein after treatment were lower than that before. However, the expression level of sIgA was higher. The differences were statistically significant. Conclusions Some differentially expressed saliva proteins of OLP before and after Huashi Xingyu Qingre decoction therapy are characterized, and they may play a vital part in the occurrence and development of OLP.

Objective Oral lichen planus ( OLP) is a common complaint in the oral mucosa , for which there is no definite therapy hitherto.This article aimed to investigate the possible effect of Huashi Xingyu Qingre Decoction (HXQD) on OLP. Methods This study included 30 randomly selected cases of OLP treated with HXQD .Fasting venous blood and total serum protein were obtained before and after medication for screening and identification of OLP-related differential proteins by two-dimensional fluorescence differ-ence gel electrophoresis (2D DIGE) and liquid chromatography-mass spectrometry (LC-MS), followed by Western blot validation . Results Haptoglobin , antithrombin Ⅲ, C1 complement , and vitamin D binding protein were differentially expressed in the serum of the OLP patients before and after treated with HXQD .Compared with the baseline , the expression of haptoglobin was significantly in-creased (103.086 ±27.536 vs 159.704 ±24.228, P<0.05) while that of antithrombin Ⅲ remarkably decreased after treatment (150.00 ±54.04 vs 98.00 ±28.04, P<0.05). Conclusion Haptoglobin, antithrombin Ⅲ, C1 complement, and vitamin D binding protein are differentially expressed in the serum of OLP patients before and after HXQD medication , which may be associated with the development and progression of OLP .

To construct and validate a deep learning-based bone age prediction model for children living in both plain and highland regions.

To establish a rat model of rheumatoid arthritis (RA) with kidney deficiency syndrome.

Objective To investigate the cognitive status of the risk of formula milk in rooming-in pregnant women .Methods Two hundred and ten pregnant women in rooming-in of tertiary A maternity hospital were surveyed by the self-designed questionnaire on the cognitive status of the risk of formula milk .Results The cognition of the risk of formula milk in rooming-in 210 pregnant women was at low level , and the percent of pregnant women who did not know the risk of the formula milk including the increase of the otitis media and ear infections, asthma and cardiovascular diseases in infants and children were respectively 68.6%, 67.3% and 64.3%.The percent of pregnant women who did not know that the formula milk increased the risks of rheumatoid arthritis in pregnant women , maternal diabetes , mothers ’ tension and anxiety were respectively 67.1%, 57.8%and 57.2%.The percent of pregnant women who knew that the formula milk increased the risk of nutritional deficiencies and breast cancer in the pregnant women were respectively 43.4% and 41.9%. Univariate analysis showed that attending to the breast-feeding lesson and understanding the breast-feeding benefits could improve the awareness of the risk of formula milk in pregnant women .Conclusions The cognitive degree of the risk of formula milk in rooming-in pregnant women is at low level at present .We should establish the standardized training , and increase the related courses of health education during the pregnant , and propagate the risk of formula milk through multi-channel, and improve the awareness of the risk of formula milk in pregnant women , and establish the informed consent system before the use of formula milk .

The development of medical technology has prolonged the life of patients, but in the face of incurable but imminent death, hospice care is needed to help patients die comfortably, peacefully and with dignity. As important recipients of hospice care, minors need special attention because of their particularity in physiology, psychology and social roles. Suggestions on the Standard of Hospice Care Procedure for Minors makes up for the deficiency that the existing standards do not target minors as a special group, standardizes the process of hospice care, and plays a role in protecting the rights and interests of minors.

Objective:To summarize and elucidate the impact of ambient air pollution on biological aging among middle-aged and older adults.Methods:"Air pollution""Biological age""Epigenetic age""Biological aging"and"Epigenetic aging", as well as specific names of air pollutants and biological age were used as search keywords. This study searched the databases of PubMed and Web of Science for eligible English articles and CNKI, CQVIP, Wanfang, CBM, CSTP and other Chinese databases for eligible Chinese articles from inception until June 30, 2023. The language was limited to Chinese and English.Results:Among the 14 included articles, five studies investigated the impact of air pollution on DNA methylation age using different algorithms, while six studies explored the relationship between air pollutants and telomere length. Six studies focused on frailty as an outcome, and an additional study revealed the relationship between fine particulate matter (PM 2.5) and its components with composite indicator age (KDM age). The results indicated that, although different forms of biological ages were susceptible to different ambient air pollutants at different degrees, previous studies had consistently found that the increased levels of PM 2.5 and one of its major components, black carbon (BC), could significantly accelerate the biological aging of middle-aged and older adults. Similar trends were observed with nitrogen oxides (NO x) and ozone (O 3) but with relatively limited evidence. Conclusion:Major air pollutants could accelerate the biological aging of middle-aged and older adults.

Objective:To summarize and elucidate the impact of ambient air pollution on biological aging among middle-aged and older adults.Methods:"Air pollution""Biological age""Epigenetic age""Biological aging"and"Epigenetic aging", as well as specific names of air pollutants and biological age were used as search keywords. This study searched the databases of PubMed and Web of Science for eligible English articles and CNKI, CQVIP, Wanfang, CBM, CSTP and other Chinese databases for eligible Chinese articles from inception until June 30, 2023. The language was limited to Chinese and English.Results:Among the 14 included articles, five studies investigated the impact of air pollution on DNA methylation age using different algorithms, while six studies explored the relationship between air pollutants and telomere length. Six studies focused on frailty as an outcome, and an additional study revealed the relationship between fine particulate matter (PM 2.5) and its components with composite indicator age (KDM age). The results indicated that, although different forms of biological ages were susceptible to different ambient air pollutants at different degrees, previous studies had consistently found that the increased levels of PM 2.5 and one of its major components, black carbon (BC), could significantly accelerate the biological aging of middle-aged and older adults. Similar trends were observed with nitrogen oxides (NO x) and ozone (O 3) but with relatively limited evidence. Conclusion:Major air pollutants could accelerate the biological aging of middle-aged and older adults.

Tinea of vellus hair is caused by dermatophyte infection of vellus hairs, and commonly affects children. It usually occurs on the face, and clinically manifests as annular or semi-annular erythema gradually spreading to the surrounding area, with central clearing and a slightly elevating border covered with papules and papulovesicles. Intense inflammation, which may manifest as pustules, erosions, exudation, scales and crusts, can be observed in patients with severe tinea of vellus hair. Direct microscopy of fungi showed abundant hyphae and/or spores on vellus hairs. Topical antifungal therapy is usually ineffective, and systemic antifungal therapy should be considered. In order to reduce the high rate of missed diagnosis and misdiagnosis, and to improve clinicians′ understanding of this disease, this review summarizes the incidence, clinical manifestations, diagnosis and treatment of tinea of vellus hair.

Chronic visceral hypersensitivity is an important type of chronic pain with unknown etiology and pathophysiology. Recent studies have shown that epigenetic regulation plays an important role in the development of chronic pain conditions. However, the role of miRNA-325-5p in chronic visceral pain remains unknown. The present study was designed to determine the roles and mechanism of miRNA-325-5p in a rat model of chronic visceral pain. This model was induced by neonatal colonic inflammation (NCI). In adulthood, NCI led to a significant reduction in the expression of miRNA-325-5p in colon-related dorsal root ganglia (DRGs), starting to decrease at the age of 4 weeks and being maintained to 8 weeks. Intrathecal administration of miRNA-325-5p agomir significantly enhanced the colorectal distention (CRD) threshold in a time-dependent manner. NCI also markedly increased the expression of CCL2 (C-C motif chemokine ligand 2) in colon-related DRGs at the mRNA and protein levels relative to age-matched control rats. The expression of CXCL12, IL33, SFRS7, and LGI1 was not significantly altered in NCI rats. CCL2 was co-expressed in NeuN-positive DRG neurons but not in glutamine synthetase-positive glial cells. Furthermore, CCL2 was mainly expressed in isolectin B4-binding- and calcitonin gene-related peptide-positive DRG neurons but in few NF-200-positive cells. More importantly, CCL2 was expressed in miR-325-5p-positive DRG neurons. Intrathecal injection of miRNA-325-5p agomir remarkably reduced the upregulation of CCL2 in NCI rats. Administration of Bindarit, an inhibitor of CCL2, markedly raised the CRD threshold in NCI rats in a dose- and time-dependent manner. These data suggest that NCI suppresses miRNA-325-5p expression and enhances CCL2 expression, thus contributing to visceral hypersensitivity in adult rats.