Objective To make early diagnosis of occupational bladder cancer in benzine-exposured worker in China. Methods An epidemicological investigation was made for a total of 1 796 benzidine-exposured worker.All the urine sample were tested for cytology,DNA G-Actin and M344 tumor associated antigen by PaP and QFIA methods.Clinical studies were then carried out for the high risk workers. Results 30 cases of bladder cancer and 1 pelvic cancer were found(1.73%),all of which were transitional cell carcinoma.There were 3 grade Ⅰ tumors,10 grade Ⅱ,18 grade Ⅲ.For the high risk workers,the time elapsed for the occurrence of clinical cancer was 18 months in average and the average patent time of occupational bladder cancer was 21.2 years (5～30 years). Conclusions Pap cytology and QFIA are beneficial to the early diagnosis and screening of occupational bladder cancer.