1.Effect of dexamethasone combined with clomiphene on serum sex hormone levels in polycystic ovary syndrome
Meifeng XU ; Qing WEI ; Ting HE
Chinese Journal of Biochemical Pharmaceutics 2017;37(1):235-238
Objective To investigate the effect of dexamethasone combined with clomiphene on serum sex hormone levels in patients with polycystic ovary syndrome. Methods 80 pregnancies with polycystic ovary syndrome treated in our hospital from January 2013 to December 2015 were selected and they were randomly divided into observation group and control group with 40 cases in each group. The observation group was given dexamethasone combined with clomiphene, while the control group was given simple clomiphene. Compared the curative effect and the change of the levels of progesterone and estradiol before and after treatment for three months. Results After treatment, the total effective rate of the observation group was 95.00%, higher than the total effective rate of the control group was 72.50%, the difference was statistically significant (P<0.05), compared the levels of progesterone(P), estradiol(E2), luteinizing hormone(LH) and LH/FSH in the observation group and the control group, the difference was statistically significant (P<0.05), after treatment, both levels were higher than before treatment. The total ovulation rate of the observation group was 90.00%, higher than the total ovulation rate of the control group was 72.50%, the difference was statistically significant (P<0.05). The level of progesterone and estradiol in serum of polycystic ovary syndrome treated by dexamethasone combined with clomiphene had a obvious positive correlation (r=0.746, 0.812, 0.731 and 0.796, P<0.05). Conclusion Dexamethasone combined with clomiphene treatment of polycystic ovary syndrome than using only clomiphene treatment is good, which can also improve serum hormone levels in patients, effectively improve the patient's endocrine abnormalities.
2.Investigation and Analyses on123Kinds of Eye Drops Specifications
Yu LI ; Yuejiao LIN ; Meifeng HE ; Yandong WANG
China Pharmacy 2001;0(09):-
OBJECTIVE:To provide suggestions for the improvement of eye drops specifications(EDS)so as to promote safe and effective use of eye drops.METHOD:123kinds of EDS in18categories in our hospital pharmacy were investigated and analyzed.RESULTS:Several important contents in the specifications like pharmacology and toxicology,pharmacokinetics,ad-verse reactions,contraindications,precautions,medications for pregnant and nursing women,and that for children and senile patients,drug interactions and overdose failed to fully meet the national criteria.The quality of the imported EDS was superior to that of the domestic EDS.CONCLUSIONS:The EDS were still far from being perfect and improvement of which is neces-sary so that more detailed drug information is available for physicians,pharmacists and patients,which is conducive to safe and effective use of drugs.
3.Study on the Stability of Phacolin Eye Drops
Wei WU ; Xilan TANG ; Meifeng HE ; Maoling CHEN
China Pharmacy 1991;0(01):-
OBJECTIVE:To observe the stability of phacolin eye drops.METHODS:The content and the stability of phacolin eye drops were determined by ultraviolet spectrophotometry and the initial average rate method respectively.RESULTS:The activation energy for pyrolytic reaction of phcolin eye drops was25.681kcal/mol,the shelflife of phacolin eye drops at room temperature(25℃)and10℃were88and878days respectively.CONCLUSION:The determination of the stability of phacolin eye drops with initial average rate method is useful and simple.
4.Effects of Acupuncture-moxibustion on Monocyte Th1/Th2 Cytokine in Peripheral Blood of Patients with Perennial Allergic Rhinitis
Meifeng ZHENG ; Cheng LIN ; Liangpu ZHENG ; Furong HE ; Xuejun CUI
Journal of Acupuncture and Tuina Science 2010;8(2):85-88
Objective:To observe the effects of acupuncture-moxibustion on symptoms,signs,and Th1/Th2 cytokine,and regulation on Th1/Th2 in patients with perennial allergic rhinitis(PAR) to provide experimental basis for acupuncture treating allergic rhinitis and investigate the mechanism.Methods:Thirty patients with allergic rhinitis were treated with acupuncture-moxibustion,and 30 healthy cases were the control.Clinical symptoms,signs,and nasal mucosa were observed,and contents of IL-4,IFN-γ,and granulocyte-macrophage colony stimulating factor (GM-CSF) in the supernatant of peripheral blood monocyte cultured with PHA by ELISA,before treatment,after one course of treatment,and after two courses of treatment.Results:Before the treatment,the contents of IL-4,and GM-CSF were higher (P<0.01) and IFN-γ was lower (P<0.01) in the PAR cases than in the control cases.In the PAR cases,the content of GM-CSF was positive correlation with that of IL-4 (P<0.01),and negative correlation with IFN-γ (P<0.01).After the treatment,contents of IL-4 and GM-CSF were lowed (P<0.01),content of IFN-γ was increased (P<0.05),and all IL-4,GM-CSF and IFN-γ had no difference compared with those in the control cases.Conclusion:Acupuncture moxibustion could improve the symptoms and signs in PAR patients,enhance the immune function.It may be achieved by regulating the expression of Th1 and Th2,correcting the imbalance of Th1/Th2.
5.Surgical effect of laparoscopic anterior resection of rectal carcinoma with high ligate or low ligate inferior mesenteric artery: A Meta-analysis
Jiankai SHEN ; Zhanwu JIANG ; Jie AN ; Meifeng HE ; Zhenjie MA
International Journal of Surgery 2017;44(12):841-847
Objective To evaluate the surgical effect of laparoscopic anterior resection of rectal carcinoma with or without the left colic artery.Methods Through computer searching PubMed,Cochrane Library,Embase,WanFang Date and CNIK Date.Handsearching the Gray literature simultaneously.The randomized controlled trails and clinical controlled trials comparing low ligation with high ligation the inferior mesenteric artery in laparoscopic anterior resection of rectal carcinoma were colected.Data were screened,extracted and evaluated by two reviewers independently.Meta-analyses were conducted using the sofeware STATE12.0.The standardized mean difference were calculated for continuous data and odds ratio for dichotomous and the point estimates of each effect quantity and 95% confidence interval were calculated.TheI2 test was used to test the heterogeneity of the inchuded literature:if the heterogeneity was small,the fixed effects model was adopted.otherwise,the random effects models was adopted and explored the heterogeneity by sensitivity analysis or subgroup analysis.Results Forteen articles including 15 researches were met the selection criteria.The Meta-analyses showed that the grope of low ligation had lower rate of anastomotic fistula (OR =0.394,95 % CI:0.251-0.616,P =0.000) and longer opration time (SMD =0.361,95%CI:0.242-0.480,P =0.000).There is no statistical differences in the number of lymph node dissection besides the root of IMA (SMD =-0.017,95% CI:-0.130-0.097,P =0.772) intraoperative biood loss (SMD =0.083,95 % CI:-0.048-0.214,P =0.213).Conclusions In spite of reserved the left colic artery had longer operation time it can reducedrate of anastomotic fistula effectively and there is no statistical differences in the number of lymph node dissection besides the root of IMA,intraoperative blood loss.It is possible to promote the use of reserved the left colic artery in laparoscopic anterior resection of rectal carcinoma.
6.Clinical research on the expression of X chromosome coupled ZFX expression in the serum and pathology of NSCLC patients
Dongtao WANG ; Lifei XING ; Meifeng SUN ; Gangfeng HE
Clinical Medicine of China 2017;33(12):1116-1121
Objective To discuss the expression of X chromosome coupled zinc finger protein(ZFX) in the serum and pathology of patients with advanced non-small cell lung cancer before and after treatment and its evaluation in the chemotherapy efficacy.Methods Forty cases(NSCLC group)with non-small cell lung cancer treated in Baotou Central Hospital from January 2013 to October 2014 were retrospectively analyzed.The control group included 40 normal people who may have tumor by normal physical examination.Based on the blood tests of research subjects before and after treatment,the peripheral blood ZFX content was detected by the quantitative detection, ZFX expression was detected by tissue morphological identification and immunohistochemical methods.Results The level of adenocarcinoma ZFX serum was(15.32± 2.01)μg/L, squamous cell carcinomas ZFX serum level was(11.65±4.12)μg/L,the difference between the two groups was statistically significant(t=3.216,P<0.05); the ZFX serum level of non-small cell lung cancer group was (17.55±0.37)μg/L before treatment,and was(6.35± 0.06)μg/L after treatment which was significantly lower,the difference was statistically significant(t=188.97,P<0.05); the serum level of non-small cell lung cancer group before treatment was(17.55±0.37)μg/L,after treatment was(6.35±0.06)μg/L,compared with (2.29± 0.01)μg/L,(2.29 ± 0.01)μg/L in the control group,the differences were statistically significant (before treatment:t=260.75,after treatment t=422.14,P<0.05); the expression of ZFX in adenocarcinoma was(15.32±2.01)ug / L,higher than that of squamous cell carcinoma((11.65±4.12)μg/L),the difference was statistically significant(t=3.216,P<0.05);the expression of ZFX in CR+PR group before treatment was (17.35±0.46)μg/L,higher than that after treatment((6.24±0.11)μg/L),the difference was statistically significant(t=142.88,P<0.05).Conclusion The expression of ZFX in peripheral blood serum and pathology may be a marker for the diagnosis of non-small cell lung cancer,and it has guiding significance for the diagnosis and curative effect evaluation of lung cancer.
7.Literature analysis on moxibustion at Yongquan (KI 1) for insomnia.
Furong HE ; Baixiao ZHAO ; Meifeng ZHENG
Chinese Acupuncture & Moxibustion 2018;38(5):5413-5414
OBJECTIVETo summarize and analyze the clinical literature regarding moxibustion at Yongquan (KI 1) for insomnia, and to provide clinical evidence of moxibustion at Yongquan (KI 1) for insomnia.
METHODSWith "moxibustion" "acupuncture-moxibustion" "Yongquan (KI 1)" "insomnia" and "sleep disorder", etc. as key terms, the clinical literature regarding moxibustion at Yongquan (KI 1) for insomnia was retrieved in CNKI, and VIP databases, and reviewed, summarized and analyzed.
RESULTSA total of 27 clinical papers were retrieved, including 14 randomized controlled trials. In recent years, the number of clinical papers had increased. Few papers selected moxibustion at Yongquan (KI 1) as independent treatment for insomnia; most papers combined moxibustion with acupuncture, massage, etc. Moxibustion was commonly manipulated by patients or family members, and few papers applied moxibustion instruments to make the manipulation easier and safer.
CONCLUSIONMoxibustion at Yongquan (KI 1) for insomnia has attracted more and more attention. It is suggested to apply convenient and safe moxibustion instruments in clinical treatment, which is benefit to clinical generalization, but also provides convenient manipulation for further study regarding its clinical effect and mechanism.
Acupuncture Therapy ; Humans ; Massage ; Moxibustion ; Randomized Controlled Trials as Topic ; Sleep Initiation and Maintenance Disorders ; therapy
8.Effect of nasal parasitism by Demodex mites on nasal skin microbiome in patients with rosacea
Caimei ZHONG ; Sihua HE ; Weifeng ZHAO ; Jianneng ZHONG ; Meifeng ZHOU ; Yuhua DENG
Chinese Journal of Dermatology 2020;53(5):345-351
Objective:To investigate relationships between nasal parasitism by Demodex mites and nasal skin microbiome in patients with rosacea. Methods:From May 2017 to June 2019, 14 patients with rosacea, including 8 with early-stage rosacea and 6 with intermediate-stage rosacea, and 14 human controls with healthy facial skin were collected from Department of Dermatology, Shunde District Center for Prevention and Cure of Chronic Disease of Foshan City. Microbial samples were collected from the nasal alar and nasolabial folds of the subjects. Then, DNA was extracted from the samples, and subjected to metagenomic sequencing and bioinformatics analysis. Relative abundance of strains was estimated by using composition ratios of Demodex mites and microbial reads. Shannon index was calculated to evaluate α diversity of microbiome, and principal component analysis (PCA) was performed to assess β diversity based on relative abundance of microbial species. Enumeration data were compared by using two-independent-sample t test, and relationships between nasal Demodex mites and skin microbiome were analyzed by Pearson correlation analysis. Results:The relative abundance of nasal Demodex mites was significantly higher in the rosacea group (1.647% ± 0.389%) than in the healthy group (0.448% ± 0.089%, t = 2.92, P = 0.007) . The relative abundance of Demodex mites was negatively correlated with the relative abundance of bacteria ( r = -0.95, P < 0.001) , and positively correlated with the relative abundance of fungi ( r = 0.76, P < 0.001) . The Shannon indices of nasal bacterial and fungal communities were significantly higher in the rosacea group (0.91 ± 0.17, 1.261 ± 0.045, respectively) than in the healthy group (0.47 ± 0.12, 0.549 ± 0.071, t = 2.17, 8.48, respectively, both P < 0.05) ; PCA showed that the β diversity of bacterial communities significantly differed between the rosacea group and healthy group ( t = 2.32, P = 0.029) , while no significant difference in the β diversity of fungal communities was observed between the two groups ( t = 0.82, P = 0.461) . In addition, the relative abundance of Demodex mites was significantly higher in the patients with intermediate-stage rosacea than in those with early-stage rosacea ( t = 6.56, P < 0.001) ; there was no significant difference in the Shannon indices of nasal bacterial or fungal communities between the two patient groups (both P > 0.05) ; PCA showed that the β diversities of bacterial and fungal communities significantly differed between the two patient groups (both P < 0.05) . Conclusion:Parasitism of Demodex mites on the nasal skin may affect nasal microbial community structure.
9. Diagnostic value of optical imaging combined with indocyanine green-guided sentinel lymph node biopsy in gastric cancer: a meta-analysis
Meifeng HE ; Zhanwu JIANG ; Zhiwei HAO ; Jie AN ; Jian ZHAI ; Jiankai SHEN
Chinese Journal of Gastrointestinal Surgery 2019;22(12):1196-1204
Objective:
To systematically evaluate the diagnostic value of optical imaging combined with indocyanine green (ICG)-guided sentinel lymph node (SLN) biopsy in gastric cancer, and to identify potential factors that would influence diagnostic accuracy.
Methods:
Study was carried out by searching the electronic database of PubMed, Embase, Medline, Web of Science, and the Cochrane Library with keywords as "gastric/stomach" and "cancer/carcinoma/tumor/tumour/adenocarcinoma/neoplasm" and "sentinel lymph node" and "near-infrared/NIR or fluorescent imaging" and "indocyanine green/ICG" . Literature inclusion criteria: (1) gastric cancer clinical stage was cT0-3; (2) clinical stage determined by at least 2 kinds of imaging modalities; (3) optical imaging (near-infrared or fluorescence imaging) combined with ICG-guided SLN biopsy; (4) prospective study to predict lymph node metastasis; (5) intraoperative or postoperative pathology for all lymph nodes removed; (6) patients number in the literature >10 cases. Exclusion criteria: (1) patients with a history of ICG allergy or chemoradiotherapy; (2) previous history of endoscopic mucosal resection or endoscopic submucosal dissection; (3) patients with a variety of gastrointestinal tumor; (4) case reports, conference abstracts, clinical guidelines, editorials, reviews, meta-analysis and correspondence letters; (5)
10.Theory and Analysis of Pharmacokinetic and Chromatokinetics Dose-time Characterization Methods in Traditional Chinese Medicine
Ru QIAO ; Peng HE ; Qijun HE ; Haiying LI ; Meifeng XIAO ; Kaiwen DENG ; Fuyuan HE
Chinese Journal of Experimental Traditional Medical Formulae 2024;30(22):178-186
ObjectiveTo establish a theoretical system of pharmacokinetic and spectrokinetic dose-time characterization of traditional Chinese medicine(TCM). By analyzing the pharmacokinetic and spectrokinetic behaviors of Lonicerae Flos, Houttuyniae Herba injection, Lonicerae Japonicae Flosand Buyang Huanwutang, this paper compared the similarities and differences of the three methods for characterizing the dose-time relationship, namely half-life, statistical moment and statistics, in order to find the most suitable method for characterizing the relationship. MethodTen mice were randomly selected from 100 Kunming mice as the blank group, and the remaining mice were coated with xylene in the auricle to establish the acute inflammation model of ear swelling. After successful modeling, the mice were gavaged with aqueous extract of Lonicerae Flos(30 g∙kg-1), and the blank group was gavaged with an equal volume of normal saline. The plasma of mice was collected at different time points to determine the content changes of components. At the same time, the pharmacokinetic results of Houttuyniae Herba injection, Lonicerae Japonicae Flos and Buyang Huanwutang were included, and the pharmacokinetic and spectrokinetic parameters were calculated. Then the difference in the time of calculating 95% total component content of metabolism by half-life method, statistical moment method and statistical method was compared. ResultOn the basis of the half-life method, the mathematical expressions of statistical moment method and statistical method suitable for the characterization of dose-time relationship of multi-component system of TCM were established. The results showed that the pharmacokinetic parameters of the individual components in Lonicerae Flos varied, with cryptochlorogenic acid and rutin showing a two-compartment model and the other components showing a one-compartment model. After calculation of spectrokinetic similarity, the metabolic patterns among the components contained in Houttuyniae Herba injection, Lonicerae Japonicae Flos, Lonicerae Flos and Buyang Huanwutang were different and varied greatly in vivo. The time to metabolize 95% of the total components of the four research subjects in vivo was calculated by the half-life method, statistical moment method and statistical method, and it was found that the difference between statistical moment method and half-life method was large, and the difference between statistical moment method and statistical method was small. ConclusionStatistical method not only reflects the characteristics of statistical moment method, characterizes the dispersion degree of each component, but also can be associated with fingerprint to form spectrokinetics, characterizing the dose-time relationship of 95% of drug components, which is a more desirable method to characterize the dose-time relationship of the component groups in TCM.