1.Analysis of 89 amniotic samples using fluorescent in situ hybridization.
Ping WEI ; Yun-xing LI ; Lan ZENG ; Chun CHEN ; Sheng-fang QIN ; Xue-yan WANG ; Na XI ; Shu-qin TANG ; Mei LENG ; Chun-lan LIU
Chinese Journal of Medical Genetics 2013;30(2):214-217
OBJECTIVETo assess the value of fluorescent in situ hybridization (FISH) for detecting common chromosome aneuploidies in interphase nuclei of amniotic fluid cells.
METHODSEighty two uncultured amniotic fluid samples and supernatants from 2 successfully and 5 unsuccessfully cultured amniotic fluid samples were analyzed with FISH. Results from standard cytogenetic analysis of 79 uncultured amniotic fluid samples and 2 successfully cultured amniotic fluid samples were compared with FISH results.
RESULTSAll of the 89 samples were succeeded analyzed with FISH. Positive findings included 3 cases with trisomy 21, 1 case with 47, XYY and 1 case with 69, XXX, which were consistent with results of karyotype analysis.
CONCLUSIONFISH is a rapid and accurate method for prenatal diagnosis, and can also provide a remedy to failed amniotic fluid cells culture.
Adult ; Amniotic Fluid ; cytology ; Cell Culture Techniques ; Female ; Humans ; In Situ Hybridization, Fluorescence ; methods ; Karyotyping ; Pregnancy
2.Establishment and evaluation of classification criteria for early rheumatoid arthritis
Jinxia ZHAO ; Yin SU ; Xiangyuan LIU ; Hua YE ; Ru LI ; Qinghua ZOU ; Yongfei FANG ; Huaxiang LIU ; Xingfu LI ; Jialong GUO ; Liqi BI ; Fei GU ; Lingyun SUN ; Yifang MEI ; Zhiyi ZHANG ; Lina CHEN ; Ping ZHU ; Guangtao LI ; Zhuoli ZHANG ; Xiaomei LENG ; Yan ZHAO ; Li JIANG ; Hejian ZOU ; Yi ZHAO ; Yi LIU ; Lei LI ; Hongyuan WANG ; Zhanguo LI
Chinese Journal of Rheumatology 2012;16(10):651-656
Objective To establish new classification criteria for early rheumatoid arthritis (E-RA) based on large samples of early inflammatory arthritis patients and to evaluate the value of this criteria in China.Methods Patients who had arthritic complaints with disease duration less than one year were enrolled.They were divided into RA group and non-RA group according to the clinical diagnosis by experienced rheumatologists.The clinical and laboratory parameters were analyzed and those with high sensitivity or specificity were selected as the new classification criteria.Statistical analysis was carried out by using t test,x2 test and Logistic regression.Results ① A total of 803 patients with early inflammatory arthritis were included in this study.Five hundreds and fourteen patients were diagnosed as early RA and 251 were diagnosed as other rheumatic diseases,and the diagnosis of 38 patients remained unestablished by the end of follow-up.② New E-RA classification criteria were established based on the parameters with high sensitivity and/or specificity.The sensitivity of the new E-RA criteria was 84.4%,which was higher than 1987 ACR criteria (58.0%),while the corresponding specificities were similar,which were 87.4% and 93.6% respectively.③ Compared with the complex scoring system of 2010 ACR/EULAR criteria,the E-RA criteria was more simple and practical.The diagnostic sensitivity and specificity of E-RA criteria were higher than those of 2010 ACR/EULAR criteria reported in the literatures.④ New classification criteria based on scoring system using Logistic regression analysis was established.The sensitivity of this criteria was 86.4%,which was higher than 1987 ACR criteria (58.0%).Conclusion The diagnostic value of the E-RA criteria developed in this study for early RA is better than 1987 ACR criteria,and is more simple than 2010 ACR/EULAR criteria.It may be used as a new classification criteria for early RA diagnosis.
3.Study on preventive and therapeutic effects of Erzhi Pills on mice with Parkinson's disease induced by MPTP.
Xin-Fang WU ; Leng-Xin DUAN ; Xiao-le GAO ; Man-Lin GUO ; Dong-Mei WANG
China Journal of Chinese Materia Medica 2019;44(19):4219-4224
The aim of this study was to investigate the preventive and therapeutic effects of Erzhi Pills on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine( MPTP)-induced Parkinson's disease( PD) in mice,and explore its possible mechanism of action. Mice were intraperitoneally injected with MPTP( 30 mg·kg-1,0. 01 m L·g-1) once daily to induce PD for 8 days. In the treatment group,Erzhi Pills were given by intragastric administration( 2. 5 g·kg-1,once daily for 30 days). The normal group received an equal volume of normalsaline. In terms of behavior,the limb movement coordination ability of the mice was detected by climbing,hanging and swimming experiments. The spatial learning and memory ability of the mice was detected by Morris water maze test. The content of MDA,as well as the activity of GSH-PX and SOD were determined in mice serum. Western blot was used to detect the protein expression levels of TH,MAOB and apoptosis-related factors CHOP and caspase-12 in brain tissues. Immunohistochemistry was used to detect the expression of TH in section of brain tissues in mice. The results showed that in behavioral aspects,as compared with the model group,the scores of limb movement ability as well as scores of spatial learning and memory ability were significantly improved in the treatment groups( P<0. 05). In terms of serological indicators,as compared with the model group,the activities of SOD and GSH-PX were significantly increased in the serum of treatment groups,and the content of MDA was significantly decreased( P<0. 05). The results of Western blot showed that as compared with the model group,the protein levels of TH in the brain tissues of the mice in treatment group were significantly up-regulated,while the protein levels of MAOB and apoptosis-related factors CHOP and caspase-12 were significantly down-regulated( P<0. 05). The results of immunohistochemistry showed that the number of TH positive cells in the brain tissues of the mice in the treatment group was significantly increased as compared with the model group( P<0. 05). In summary,Erzhi Pills have a certain preventive and therapeutic effect on MPTP-induced PD mice,which can significantly improve the limb motor coordination ability and spatial learning and memory ability of PD mice. Its mechanism may be related to down-regulating the expression of apoptosis-related factors CHOP and caspase-12,reducing the dopaminergic neuron damage and inhibiting dopaminergic neuronal apoptosis.
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
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Animals
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Disease Models, Animal
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Drugs, Chinese Herbal/pharmacology*
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Mice
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Mice, Inbred C57BL
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Neuroprotective Agents/pharmacology*
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Parkinson Disease
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Substantia Nigra
4.Aortic Dissection Mimicking Acute Myocardial Infarction
Jun LIU ; Yong-cong HE ; Xiao-xue ZHANG ; Jia CHEN ; Mei-fang LENG ; Zuo-yi DU
Journal of Sun Yat-sen University(Medical Sciences) 2019;40(1):110-116
【Objective】To explore the clinical characteristics of aortic dissection involved in coronary artery in order to avoid misdiagnosis,mistreatment,and take correct treatment methods in time.【Methods】Twelve cases of aortic dissection(AD)manifesting as acute myocardial infarction(AMI)were analyzed retrospectively and followed up.【Results】 A total of 12 cases were enrolled from 288 cases of AD patients,of which 2 cases(16.7%)were inferior wall myocardial infarction,1 cases(8.3%)were anterior wall myocardial infarction,9 cases(75%)were non ST segment elevation myocardial infarction,male patients was significantly predominant over female,leading to the male/female ratio of 3∶1,the age was 43~76(56.9±12.2)years. Up to 66.7% of patients had a history of hypertension. 11 patients(91.7%)were admitted to hospital for different degrees of chest pain,with back pain in 9 cases(75%),syncope in 4 cases(33.3%),hypotension in 6 cases(50%),and irritability in 7 cases(58.3%). All the myocardial enzymes were positive in the laboratory and significantly elevated D-dimer;3 cases were performed coronary arteriography but not found the opening of coronary artery. AD was confirmed by transthoracic echocardiography and chest CTA. 3 cases of surgery were performed,1 cases failed and died within 24 hours,and 2 cases were successful. The average follow-up 5 hours to 12 months,2 cases survived and the remaining 10 cases died.【Conclusions】Myocardial infarction caused by aortic dissection is rare in clinic ,but it has critical and high mortality rate,and it is easily misdiagnosed,with poor prognosis. Early diagnosis and timely operation can improve the prognosis. In the case of AMI with back pain,irritability,and abnormal rise of D two polymer,the disease should be highly suspected.
5.A comparative analysis of the clinical symptoms of benign paroxysmal positional vertigo between older and young and middle-aged patients.
Rui Le FANG ; Qi LENG ; Yan WANG ; Mei Mei CHEN ; Yu CUI ; Xiao bing WU ; Yi JU
Chinese Journal of Internal Medicine 2023;62(7):802-807
Objective: To compare the differences in clinical symptoms and the time required for diagnosis of benign paroxysmal positional vertigo (BPPV) between older patients and young and middle-aged patients in the structured inquiry of dizziness history. Methods: The medical records of 6 807 patients diagnosed with BPPV from the Vertigo Database of Vertigo Clinical Diagnosis, Treatment, and Research Center of Beijing Tiantan Hospital, Capital Medical University, between January 2019 and October 2021 were retrospectively analyzed. The data included basic demographic information, clinical symptoms in a structured medical history questionnaire, and the time interval from the appearance of BPPV symptoms to diagnosis consultation. The patients were divided into the young and middle-aged group (<65 years old) and the older group (≥65 years old). The differences in clinical symptoms and consultation time were compared between these two groups. Categorical variables were represented by numbers (%), and compared using Chi-squared tests or Fisher's exact probability test for analysis; whereas, continuous variables conforming to normal distribution were represented by mean±standard deviation. Both data groups were compared and analyzed by Student's t-test. Results: The mean age of the older group was 65-92 (71±5) years, while the mean age of the middle-aged group was 18-64 (49±12) years. The incidence of vertigo (42.5% vs. 49.1%, χ2=23.69, P<0.001); vertigo triggered by changes in position of the head or body (52.4% vs. 58.7%, χ2=22.31, P<0.001); and autonomic symptoms (10.1% vs. 12.4%, χ2=7.09, P=0.008) were lower, but hearing loss (11.8% vs. 7.8%, χ2=27.36, P<0.001) and sleep disorders (18.5% vs. 15.2%, χ2=11.13, P=0.001) were higher in the older group than in the young and middle-aged group. The time from the appearance of dizziness to diagnosis was commonly longer in the older patient group than the other group (55.0% vs. 38.5%, χ2=55.95, P<0.001). Conclusions: Older patients with BPPV have more atypical symptoms and complex concomitant symptoms than young and middle-aged patients. For older patients with dizziness, positional testing is needed to confirm the possibility of BPPV even if the clinical symptoms are atypical.
Middle Aged
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Humans
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Aged
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Aged, 80 and over
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Adolescent
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Young Adult
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Adult
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Benign Paroxysmal Positional Vertigo/therapy*
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Dizziness/diagnosis*
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Retrospective Studies
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Patients
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Surveys and Questionnaires