Objective To observe the therapetic effects of rotavirus enterocolitis by zedoary turmeric oil and glucose injection. Methods 120 cases were divided into therapatic group and control group at random,with 60 cases in each group. In the control group,ribavirin was used,while in the therapetic group,zedoary turmeric oil and glucose injection was added,without antibiotics in both groups. If dehydration were found,ORS or rehydration by vein would be used to rectify the disorder of water and electrolyte. Results In the therapetic group, it took much shorter time to stop diarrhea than the contrast group( P < 0.05 ). The difference between total disease processes of these two groups is extremely significant(P< 0.01 ). Conclusion Using zedoary turmeric oil and glucose injection with ribavirin to treat rotavirus enterocolitis has good therapetic effect.

OBJECTIVE To post correlation between urinary retention following caudal anesthesia operation and nursing countermeasures,and to supply credible gist for reducing intra-urethral cannula. METHODS A total of 146 patients with urinary retention following caudal anesthesia and anal canal operation from Jun to Oct 2003 in our hospital were analyzed. RESULTS It was obvious that patients with caudal anesthesia taken no intra-urethral cannula way to induce urination especially for adolescent.Nineteen patients taken intra-urethral cannula were with an average of(53.7 years),it was significant different between patients of different age with urinary retention taking physical methods to induce urination. CONCLUSIONS It must be thought much of taking physical methods without analgesia,irritation,and side-effect to help patient′s urination,reducing invasive operation,and avoiding iatrogenic infection.

OBJECTIVE To understand the situation about the hospital infection knowledge among externs.METHODS Using self-designed questionnaires to investigate the externs.RESULTS The cognition rate among externs about hospital infection knowledge was 56.89%,the highest rate was the answer about the best collecting time of pathogenic samples for subsequent use of antibacterials,which was 92.2%;the lowest rate was the answer about the disinfectant monitoring time limit,which was 24.4%.CONCLUSIONS Externs are seriously lack to the hospital infection knowledge.It′s necessary and urgent to strengthen the training about hospital infection knowledge among externs.

OBJECTIVE To know the situation of antimicrobial application during laparoscopic cholecystectomy in hospital and the role of surgical nurses in rational use of antibacterials to prevent the nosocomial infection.METHODS To train surgical and operating room nurses on basis of Zhejiang provincial guide scheme on clinical rational use of antibacterials and chooses the patients of department of general surgery after cholecystectomy during Jan to Jun 2006 to know their antibacterial application.RESULTS From 219 cases after laparoscopic cholecystectomy during this period,96% had rational use of antibacterials during perioperative period vs 56% in the same period of 2005 when were without surgical nurse intervention.CONCLUSIONS The active of surgical nurses is important to significantly elevate the efficacy of rational use of antibacterials during perioperative period.

OBJECTIVE:To provide reference for the timely and accurate judgment of drug fever induced by Piperacillin sodi-um and tazobactam sodium for injection and rational drug use in the clinic. METHODS:The medication,clinical manifestations, laboratory examination results and the physician treatment of 30 inpatients with drug fever induced by Piperacillin sodium and tazo-bactam sodium for injection from Sept. 2013 to Sept. 2014 were analyzed retrospectively and statistically. RESULTS:Most of the drug fever induced by Piperacillin sodium and tazobactam sodium for injection occurred in continuous 7 to 14 d medication,and the cumulative dosages were between 1.3 to 2.7 g/kg;73.3% of the 30 patients had fever during the intravenous drip,with body temperature mainly≥38.5 ℃;the elevation of eosinophil and slightly increase of serum C-reactive protein (CRP) and blood cell sedimentation rate (ESR) could be used as observation indexes of drug fever,but the leucocyte reduce could’t be suitable;the body temperature dropped to normal within 24 to 48 hours after stopped using it. CONCLUSIONS:The drug fever induced by Piperacillin sodium and tazobactam sodium for injection has no correlation with the patients’gender or age,and no special diagnos-tic criteria. But it has certain correlation with duration of medication,cumulative days and dosages and it can be used as reference of judgment with the combination of hematological examination index. Clinicians should improve the understanding and attention about drug fever to stop using suspicious drug in time.

Objective:To establish a method of organotypic cerebral culture.So as to pave the way for building some neurodegenerative disease models.Methods:Organotypic cerebral cultures were prepared from prefrontal brain of neonatal SD rats. After culturing 7 to 14 days, 3 weeks, 4 weeks and 8 weeks, respectively, cerebral slices were fixed, dehydrated and sectioned in cryostat. The sections proceeded with Nissl staining and neurofilament high molecular weight (NFH) immunohistochemical staining.The difference was observed between controls and cultured slices using normal rats as controls. Results:Nissl staining showed that pyramidal neurons in cultured slices were increased in volume and lightened in staining. The delaminating construction was clear from 1 to 4 weeks after culturing. In cultured slices, immunohistochemical staining showed that NFH positive pyramidal cells appeared on layer Ⅴ on the tenth day and on both layers Ⅴ and Ⅲ after culturing 12 days. In the control group, NFH positive pyramidal cells appeared on layer Ⅴin 5-day-old rats, and appeared on both layers Ⅴand Ⅲ in over 3-week-old rats. In cultured cerebral slices, the number of pyramidal neurons on layer Ⅴ in M1 area was invariable from 12 days to 2 months. Conclusion:Orgaotypic cerebral culture can be used to study postnatal development for neocortex and build some in vitro models for neurodegenerative diseases.

Objective To study the expression of extracellular matrix and polycystin-1 in ADPKD and their relation to cyst formation. Methods The expression of polycystin-1, fibronectin, laminin, type Ⅰ collagen, and type Ⅳ collagen were analysed in the normal kidney, fetal kidney and polycystic renal tissue by using immunohistochemical technique. Results The expression Of fibronectin, laminin, type Ⅰ collagen, and type Ⅳ collagen increased in polycystic renal tissue compared with normal kidney. The basement membrane lining cysts was markedly thickened. Type Ⅰ collagen was detected in the interstitium between cysts. Laminin, fibronectin and type Ⅳ collagen were localized in cyst basement membrane. The expression of polycystin-1 increased in polycystic renal tissue. The expression of extracellular matrix had significant correlation with the expression of polycystin-1. Conclusion The abnormal expressions of extracellular matrix and polycystin-1 exist in ADPKD. Abnormal expression of polycystin-1 may result in the alterations of extracellular matrix that is related to cyst formation.

OBJECTIVE: To study the effect of breviscapine on memory and antioxidation ability in A(342-induced Alzheimer's disease (AD) rat model. METHODS: AD rat model was established by intracerebroventricular injection of A (342. Besides, sham-operated mice served as control group. The learning and memory ability was determined by Morris water maze test. The activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) and the content of Malondialdehyde (MDA) in brain tissue were measured with spectrophotometer. RESULTS: Compared with control group, AD model significantly prolonged the latency of searching the hidden platform in directional swimming test, and decreased time spent in swimming in the target quadrant in probe test. Breviscapine dose-dependently shortened the latency of searching the hidden platform in directional swimming test, and increased the time spent swimming in the target quadrant in probe test. The activities of GSH-Px and SOD were decreased, and the MDA content was increased in AD model rats. In breviscapine treatment groups, the activities of GSH-Px and SOD heightened and MDA content decreased, and there was significant difference compared with AD model group. CONCLUSION: Breviscapine can dose-dependently ameliorate the impaired learning and memory function of AD model rats and improve antioxidation ability. Copyright 2012 by the Chinese Pharmaceutical Association.

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